Amino acid dipepetide frequency for Panteoa phage Phynn

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.765AlaAla: 5.765 ± 0.386
0.559AlaCys: 0.559 ± 0.103
4.828AlaAsp: 4.828 ± 0.331
5.099AlaGlu: 5.099 ± 0.329
2.432AlaPhe: 2.432 ± 0.228
4.955AlaGly: 4.955 ± 0.373
1.117AlaHis: 1.117 ± 0.141
4.846AlaIle: 4.846 ± 0.304
5.225AlaLys: 5.225 ± 0.307
6.198AlaLeu: 6.198 ± 0.353
1.802AlaMet: 1.802 ± 0.201
3.315AlaAsn: 3.315 ± 0.282
2.198AlaPro: 2.198 ± 0.215
2.36AlaGln: 2.36 ± 0.205
3.063AlaArg: 3.063 ± 0.215
3.982AlaSer: 3.982 ± 0.306
4.558AlaThr: 4.558 ± 0.464
5.261AlaVal: 5.261 ± 0.32
0.703AlaTrp: 0.703 ± 0.105
2.558AlaTyr: 2.558 ± 0.172
0.0AlaXaa: 0.0 ± 0.0
Cys
0.667CysAla: 0.667 ± 0.13
0.198CysCys: 0.198 ± 0.057
0.667CysAsp: 0.667 ± 0.122
0.577CysGlu: 0.577 ± 0.095
0.54CysPhe: 0.54 ± 0.106
0.649CysGly: 0.649 ± 0.114
0.324CysHis: 0.324 ± 0.075
0.685CysIle: 0.685 ± 0.11
0.883CysLys: 0.883 ± 0.14
0.721CysLeu: 0.721 ± 0.123
0.288CysMet: 0.288 ± 0.068
0.595CysAsn: 0.595 ± 0.112
0.486CysPro: 0.486 ± 0.093
0.216CysGln: 0.216 ± 0.064
0.613CysArg: 0.613 ± 0.126
0.775CysSer: 0.775 ± 0.137
0.559CysThr: 0.559 ± 0.102
0.793CysVal: 0.793 ± 0.126
0.126CysTrp: 0.126 ± 0.042
0.342CysTyr: 0.342 ± 0.075
0.0CysXaa: 0.0 ± 0.0
Asp
4.396AspAla: 4.396 ± 0.263
0.559AspCys: 0.559 ± 0.102
3.657AspAsp: 3.657 ± 0.273
3.585AspGlu: 3.585 ± 0.309
2.973AspPhe: 2.973 ± 0.222
4.576AspGly: 4.576 ± 0.339
1.135AspHis: 1.135 ± 0.131
4.594AspIle: 4.594 ± 0.311
4.054AspLys: 4.054 ± 0.319
5.333AspLeu: 5.333 ± 0.404
1.766AspMet: 1.766 ± 0.194
2.919AspAsn: 2.919 ± 0.214
2.937AspPro: 2.937 ± 0.218
2.162AspGln: 2.162 ± 0.203
3.171AspArg: 3.171 ± 0.25
4.576AspSer: 4.576 ± 0.289
3.819AspThr: 3.819 ± 0.258
4.216AspVal: 4.216 ± 0.326
1.171AspTrp: 1.171 ± 0.151
3.315AspTyr: 3.315 ± 0.226
0.0AspXaa: 0.0 ± 0.0
Glu
4.558GluAla: 4.558 ± 0.275
0.865GluCys: 0.865 ± 0.134
3.765GluAsp: 3.765 ± 0.269
4.684GluGlu: 4.684 ± 0.32
2.414GluPhe: 2.414 ± 0.218
3.765GluGly: 3.765 ± 0.239
1.063GluHis: 1.063 ± 0.12
5.423GluIle: 5.423 ± 0.335
5.207GluLys: 5.207 ± 0.308
5.945GluLeu: 5.945 ± 0.277
2.144GluMet: 2.144 ± 0.186
3.711GluAsn: 3.711 ± 0.272
1.676GluPro: 1.676 ± 0.204
2.09GluGln: 2.09 ± 0.249
3.099GluArg: 3.099 ± 0.242
4.162GluSer: 4.162 ± 0.263
3.964GluThr: 3.964 ± 0.271
4.54GluVal: 4.54 ± 0.309
1.153GluTrp: 1.153 ± 0.138
2.648GluTyr: 2.648 ± 0.228
0.0GluXaa: 0.0 ± 0.0
Phe
2.775PheAla: 2.775 ± 0.251
0.486PheCys: 0.486 ± 0.086
3.477PheAsp: 3.477 ± 0.215
2.666PheGlu: 2.666 ± 0.244
1.351PhePhe: 1.351 ± 0.174
2.847PheGly: 2.847 ± 0.227
0.595PheHis: 0.595 ± 0.102
2.955PheIle: 2.955 ± 0.24
2.576PheLys: 2.576 ± 0.247
2.847PheLeu: 2.847 ± 0.239
1.189PheMet: 1.189 ± 0.157
2.162PheAsn: 2.162 ± 0.18
1.189PhePro: 1.189 ± 0.154
1.189PheGln: 1.189 ± 0.145
1.748PheArg: 1.748 ± 0.175
2.991PheSer: 2.991 ± 0.237
2.468PheThr: 2.468 ± 0.219
3.225PheVal: 3.225 ± 0.226
0.721PheTrp: 0.721 ± 0.106
1.73PheTyr: 1.73 ± 0.226
0.0PheXaa: 0.0 ± 0.0
Gly
4.324GlyAla: 4.324 ± 0.353
0.793GlyCys: 0.793 ± 0.111
3.928GlyAsp: 3.928 ± 0.232
3.892GlyGlu: 3.892 ± 0.348
3.027GlyPhe: 3.027 ± 0.249
4.126GlyGly: 4.126 ± 0.365
0.991GlyHis: 0.991 ± 0.131
4.234GlyIle: 4.234 ± 0.263
4.72GlyLys: 4.72 ± 0.311
4.684GlyLeu: 4.684 ± 0.322
1.712GlyMet: 1.712 ± 0.16
3.639GlyAsn: 3.639 ± 0.385
0.973GlyPro: 0.973 ± 0.137
2.144GlyGln: 2.144 ± 0.219
2.883GlyArg: 2.883 ± 0.239
5.135GlySer: 5.135 ± 0.401
3.765GlyThr: 3.765 ± 0.388
4.792GlyVal: 4.792 ± 0.317
0.901GlyTrp: 0.901 ± 0.128
3.027GlyTyr: 3.027 ± 0.258
0.0GlyXaa: 0.0 ± 0.0
His
1.189HisAla: 1.189 ± 0.157
0.288HisCys: 0.288 ± 0.088
1.207HisAsp: 1.207 ± 0.181
1.189HisGlu: 1.189 ± 0.134
0.667HisPhe: 0.667 ± 0.105
0.937HisGly: 0.937 ± 0.132
0.468HisHis: 0.468 ± 0.113
1.243HisIle: 1.243 ± 0.141
0.991HisLys: 0.991 ± 0.132
1.243HisLeu: 1.243 ± 0.16
0.324HisMet: 0.324 ± 0.092
0.811HisAsn: 0.811 ± 0.127
0.973HisPro: 0.973 ± 0.14
0.468HisGln: 0.468 ± 0.099
0.883HisArg: 0.883 ± 0.123
1.027HisSer: 1.027 ± 0.113
0.739HisThr: 0.739 ± 0.12
1.189HisVal: 1.189 ± 0.139
0.252HisTrp: 0.252 ± 0.067
0.811HisTyr: 0.811 ± 0.125
0.0HisXaa: 0.0 ± 0.0
Ile
4.738IleAla: 4.738 ± 0.322
0.757IleCys: 0.757 ± 0.105
5.189IleAsp: 5.189 ± 0.263
4.522IleGlu: 4.522 ± 0.261
2.36IlePhe: 2.36 ± 0.193
3.693IleGly: 3.693 ± 0.247
1.081IleHis: 1.081 ± 0.159
3.783IleIle: 3.783 ± 0.326
4.36IleLys: 4.36 ± 0.24
4.162IleLeu: 4.162 ± 0.26
2.162IleMet: 2.162 ± 0.183
3.711IleAsn: 3.711 ± 0.246
2.829IlePro: 2.829 ± 0.204
2.252IleGln: 2.252 ± 0.22
3.711IleArg: 3.711 ± 0.282
4.198IleSer: 4.198 ± 0.257
4.036IleThr: 4.036 ± 0.236
4.594IleVal: 4.594 ± 0.325
0.631IleTrp: 0.631 ± 0.108
2.342IleTyr: 2.342 ± 0.216
0.0IleXaa: 0.0 ± 0.0
Lys
5.315LysAla: 5.315 ± 0.333
0.577LysCys: 0.577 ± 0.116
4.576LysAsp: 4.576 ± 0.274
5.207LysGlu: 5.207 ± 0.312
3.189LysPhe: 3.189 ± 0.244
4.09LysGly: 4.09 ± 0.284
1.405LysHis: 1.405 ± 0.174
4.288LysIle: 4.288 ± 0.223
4.774LysLys: 4.774 ± 0.309
5.855LysLeu: 5.855 ± 0.315
2.54LysMet: 2.54 ± 0.226
3.567LysAsn: 3.567 ± 0.245
2.63LysPro: 2.63 ± 0.2
2.919LysGln: 2.919 ± 0.234
3.153LysArg: 3.153 ± 0.272
4.18LysSer: 4.18 ± 0.277
4.234LysThr: 4.234 ± 0.323
4.288LysVal: 4.288 ± 0.269
1.045LysTrp: 1.045 ± 0.12
2.955LysTyr: 2.955 ± 0.22
0.0LysXaa: 0.0 ± 0.0
Leu
5.351LeuAla: 5.351 ± 0.318
0.865LeuCys: 0.865 ± 0.151
4.792LeuAsp: 4.792 ± 0.28
4.973LeuGlu: 4.973 ± 0.323
3.369LeuPhe: 3.369 ± 0.26
3.946LeuGly: 3.946 ± 0.259
1.423LeuHis: 1.423 ± 0.139
4.324LeuIle: 4.324 ± 0.29
5.423LeuLys: 5.423 ± 0.301
4.936LeuLeu: 4.936 ± 0.36
2.216LeuMet: 2.216 ± 0.222
4.486LeuAsn: 4.486 ± 0.269
3.297LeuPro: 3.297 ± 0.218
3.099LeuGln: 3.099 ± 0.252
4.126LeuArg: 4.126 ± 0.272
6.144LeuSer: 6.144 ± 0.289
4.486LeuThr: 4.486 ± 0.336
5.405LeuVal: 5.405 ± 0.334
0.775LeuTrp: 0.775 ± 0.113
2.955LeuTyr: 2.955 ± 0.245
0.0LeuXaa: 0.0 ± 0.0
Met
1.694MetAla: 1.694 ± 0.183
0.18MetCys: 0.18 ± 0.051
1.513MetAsp: 1.513 ± 0.172
1.892MetGlu: 1.892 ± 0.201
1.189MetPhe: 1.189 ± 0.147
1.477MetGly: 1.477 ± 0.153
0.504MetHis: 0.504 ± 0.102
1.928MetIle: 1.928 ± 0.202
3.081MetLys: 3.081 ± 0.258
1.964MetLeu: 1.964 ± 0.179
0.901MetMet: 0.901 ± 0.133
1.748MetAsn: 1.748 ± 0.183
0.919MetPro: 0.919 ± 0.114
1.045MetGln: 1.045 ± 0.15
1.405MetArg: 1.405 ± 0.155
2.504MetSer: 2.504 ± 0.196
1.531MetThr: 1.531 ± 0.174
1.639MetVal: 1.639 ± 0.192
0.27MetTrp: 0.27 ± 0.078
1.099MetTyr: 1.099 ± 0.158
0.0MetXaa: 0.0 ± 0.0
Asn
3.892AsnAla: 3.892 ± 0.32
0.432AsnCys: 0.432 ± 0.086
2.973AsnAsp: 2.973 ± 0.231
3.261AsnGlu: 3.261 ± 0.254
2.162AsnPhe: 2.162 ± 0.235
3.693AsnGly: 3.693 ± 0.255
0.919AsnHis: 0.919 ± 0.128
3.117AsnIle: 3.117 ± 0.217
3.495AsnLys: 3.495 ± 0.259
4.162AsnLeu: 4.162 ± 0.295
1.423AsnMet: 1.423 ± 0.158
2.63AsnAsn: 2.63 ± 0.232
2.63AsnPro: 2.63 ± 0.201
2.0AsnGln: 2.0 ± 0.205
2.702AsnArg: 2.702 ± 0.219
3.603AsnSer: 3.603 ± 0.274
3.729AsnThr: 3.729 ± 0.309
3.207AsnVal: 3.207 ± 0.235
0.45AsnTrp: 0.45 ± 0.085
1.639AsnTyr: 1.639 ± 0.159
0.0AsnXaa: 0.0 ± 0.0
Pro
2.576ProAla: 2.576 ± 0.251
0.378ProCys: 0.378 ± 0.082
2.702ProAsp: 2.702 ± 0.236
3.207ProGlu: 3.207 ± 0.285
1.784ProPhe: 1.784 ± 0.17
2.18ProGly: 2.18 ± 0.243
0.54ProHis: 0.54 ± 0.092
1.784ProIle: 1.784 ± 0.177
1.946ProLys: 1.946 ± 0.183
2.775ProLeu: 2.775 ± 0.268
0.595ProMet: 0.595 ± 0.098
1.676ProAsn: 1.676 ± 0.189
1.225ProPro: 1.225 ± 0.204
0.721ProGln: 0.721 ± 0.117
1.639ProArg: 1.639 ± 0.202
2.991ProSer: 2.991 ± 0.21
2.306ProThr: 2.306 ± 0.216
3.279ProVal: 3.279 ± 0.309
0.468ProTrp: 0.468 ± 0.091
1.639ProTyr: 1.639 ± 0.152
0.0ProXaa: 0.0 ± 0.0
Gln
2.648GlnAla: 2.648 ± 0.263
0.306GlnCys: 0.306 ± 0.077
1.639GlnAsp: 1.639 ± 0.178
2.396GlnGlu: 2.396 ± 0.213
1.441GlnPhe: 1.441 ± 0.168
1.369GlnGly: 1.369 ± 0.16
0.396GlnHis: 0.396 ± 0.084
2.342GlnIle: 2.342 ± 0.212
2.576GlnLys: 2.576 ± 0.213
2.576GlnLeu: 2.576 ± 0.24
1.099GlnMet: 1.099 ± 0.141
1.676GlnAsn: 1.676 ± 0.181
1.279GlnPro: 1.279 ± 0.178
1.748GlnGln: 1.748 ± 0.234
1.82GlnArg: 1.82 ± 0.204
2.0GlnSer: 2.0 ± 0.196
1.982GlnThr: 1.982 ± 0.196
2.793GlnVal: 2.793 ± 0.284
0.486GlnTrp: 0.486 ± 0.112
1.513GlnTyr: 1.513 ± 0.159
0.0GlnXaa: 0.0 ± 0.0
Arg
3.189ArgAla: 3.189 ± 0.248
0.468ArgCys: 0.468 ± 0.083
3.387ArgAsp: 3.387 ± 0.217
3.369ArgGlu: 3.369 ± 0.244
2.306ArgPhe: 2.306 ± 0.225
3.261ArgGly: 3.261 ± 0.228
0.631ArgHis: 0.631 ± 0.116
3.513ArgIle: 3.513 ± 0.299
3.783ArgLys: 3.783 ± 0.314
3.946ArgLeu: 3.946 ± 0.22
1.495ArgMet: 1.495 ± 0.167
2.973ArgAsn: 2.973 ± 0.231
1.621ArgPro: 1.621 ± 0.208
1.567ArgGln: 1.567 ± 0.2
2.378ArgArg: 2.378 ± 0.198
2.847ArgSer: 2.847 ± 0.205
2.684ArgThr: 2.684 ± 0.234
3.459ArgVal: 3.459 ± 0.252
0.793ArgTrp: 0.793 ± 0.104
1.946ArgTyr: 1.946 ± 0.203
0.0ArgXaa: 0.0 ± 0.0
Ser
4.432SerAla: 4.432 ± 0.263
0.504SerCys: 0.504 ± 0.1
4.324SerAsp: 4.324 ± 0.306
4.09SerGlu: 4.09 ± 0.25
2.847SerPhe: 2.847 ± 0.247
5.819SerGly: 5.819 ± 0.404
0.937SerHis: 0.937 ± 0.115
4.396SerIle: 4.396 ± 0.331
4.288SerLys: 4.288 ± 0.256
5.585SerLeu: 5.585 ± 0.324
1.946SerMet: 1.946 ± 0.199
3.189SerAsn: 3.189 ± 0.286
2.811SerPro: 2.811 ± 0.218
2.09SerGln: 2.09 ± 0.267
3.423SerArg: 3.423 ± 0.258
4.414SerSer: 4.414 ± 0.431
3.657SerThr: 3.657 ± 0.289
4.918SerVal: 4.918 ± 0.314
0.829SerTrp: 0.829 ± 0.128
2.829SerTyr: 2.829 ± 0.228
0.0SerXaa: 0.0 ± 0.0
Thr
4.396ThrAla: 4.396 ± 0.332
0.613ThrCys: 0.613 ± 0.105
3.639ThrAsp: 3.639 ± 0.246
3.982ThrGlu: 3.982 ± 0.275
2.072ThrPhe: 2.072 ± 0.252
4.973ThrGly: 4.973 ± 0.375
1.045ThrHis: 1.045 ± 0.129
3.964ThrIle: 3.964 ± 0.318
4.0ThrLys: 4.0 ± 0.284
4.936ThrLeu: 4.936 ± 0.314
1.549ThrMet: 1.549 ± 0.168
2.468ThrAsn: 2.468 ± 0.241
2.793ThrPro: 2.793 ± 0.235
1.838ThrGln: 1.838 ± 0.217
2.937ThrArg: 2.937 ± 0.201
3.639ThrSer: 3.639 ± 0.282
3.441ThrThr: 3.441 ± 0.309
4.342ThrVal: 4.342 ± 0.284
0.775ThrTrp: 0.775 ± 0.117
2.27ThrTyr: 2.27 ± 0.222
0.0ThrXaa: 0.0 ± 0.0
Val
5.297ValAla: 5.297 ± 0.317
1.225ValCys: 1.225 ± 0.168
4.918ValAsp: 4.918 ± 0.292
5.027ValGlu: 5.027 ± 0.327
2.865ValPhe: 2.865 ± 0.19
4.09ValGly: 4.09 ± 0.252
1.135ValHis: 1.135 ± 0.149
4.612ValIle: 4.612 ± 0.289
5.477ValLys: 5.477 ± 0.301
4.666ValLeu: 4.666 ± 0.351
1.621ValMet: 1.621 ± 0.148
3.747ValAsn: 3.747 ± 0.259
2.216ValPro: 2.216 ± 0.26
1.964ValGln: 1.964 ± 0.221
3.819ValArg: 3.819 ± 0.239
4.9ValSer: 4.9 ± 0.348
4.342ValThr: 4.342 ± 0.327
5.243ValVal: 5.243 ± 0.336
0.847ValTrp: 0.847 ± 0.133
3.009ValTyr: 3.009 ± 0.214
0.0ValXaa: 0.0 ± 0.0
Trp
0.739TrpAla: 0.739 ± 0.105
0.198TrpCys: 0.198 ± 0.063
0.955TrpAsp: 0.955 ± 0.133
0.865TrpGlu: 0.865 ± 0.14
0.685TrpPhe: 0.685 ± 0.112
0.595TrpGly: 0.595 ± 0.121
0.27TrpHis: 0.27 ± 0.063
0.721TrpIle: 0.721 ± 0.129
1.189TrpLys: 1.189 ± 0.138
0.937TrpLeu: 0.937 ± 0.136
0.432TrpMet: 0.432 ± 0.09
0.703TrpAsn: 0.703 ± 0.119
0.288TrpPro: 0.288 ± 0.064
0.504TrpGln: 0.504 ± 0.095
0.703TrpArg: 0.703 ± 0.108
0.793TrpSer: 0.793 ± 0.112
0.559TrpThr: 0.559 ± 0.088
1.045TrpVal: 1.045 ± 0.16
0.09TrpTrp: 0.09 ± 0.041
0.703TrpTyr: 0.703 ± 0.116
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.883TyrAla: 2.883 ± 0.219
0.45TyrCys: 0.45 ± 0.081
2.775TyrAsp: 2.775 ± 0.203
2.396TyrGlu: 2.396 ± 0.225
1.513TyrPhe: 1.513 ± 0.167
2.666TyrGly: 2.666 ± 0.24
0.919TyrHis: 0.919 ± 0.154
2.36TyrIle: 2.36 ± 0.179
2.738TyrLys: 2.738 ± 0.238
2.919TyrLeu: 2.919 ± 0.253
1.261TyrMet: 1.261 ± 0.14
2.396TyrAsn: 2.396 ± 0.209
1.441TyrPro: 1.441 ± 0.143
1.712TyrGln: 1.712 ± 0.196
2.342TyrArg: 2.342 ± 0.222
2.468TyrSer: 2.468 ± 0.23
2.811TyrThr: 2.811 ± 0.214
2.793TyrVal: 2.793 ± 0.242
0.486TyrTrp: 0.486 ± 0.097
1.585TyrTyr: 1.585 ± 0.216
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 266 proteins (55506 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski