Amino acid dipepetide frequency for Bacillus phage Moonbeam

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.742AlaAla: 4.742 ± 0.352
0.48AlaCys: 0.48 ± 0.094
4.053AlaAsp: 4.053 ± 0.31
4.262AlaGlu: 4.262 ± 0.35
2.486AlaPhe: 2.486 ± 0.205
4.617AlaGly: 4.617 ± 0.35
0.836AlaHis: 0.836 ± 0.136
4.888AlaIle: 4.888 ± 0.339
5.076AlaLys: 5.076 ± 0.319
5.223AlaLeu: 5.223 ± 0.35
1.379AlaMet: 1.379 ± 0.161
3.593AlaAsn: 3.593 ± 0.279
2.11AlaPro: 2.11 ± 0.358
2.319AlaGln: 2.319 ± 0.287
2.486AlaArg: 2.486 ± 0.221
4.157AlaSer: 4.157 ± 0.331
4.784AlaThr: 4.784 ± 0.344
3.781AlaVal: 3.781 ± 0.31
0.648AlaTrp: 0.648 ± 0.13
2.987AlaTyr: 2.987 ± 0.284
0.0AlaXaa: 0.0 ± 0.0
Cys
0.376CysAla: 0.376 ± 0.087
0.188CysCys: 0.188 ± 0.064
0.439CysAsp: 0.439 ± 0.125
0.397CysGlu: 0.397 ± 0.097
0.397CysPhe: 0.397 ± 0.117
0.522CysGly: 0.522 ± 0.118
0.167CysHis: 0.167 ± 0.065
0.397CysIle: 0.397 ± 0.111
0.731CysLys: 0.731 ± 0.144
0.689CysLeu: 0.689 ± 0.117
0.084CysMet: 0.084 ± 0.045
0.46CysAsn: 0.46 ± 0.11
0.334CysPro: 0.334 ± 0.096
0.23CysGln: 0.23 ± 0.067
0.48CysArg: 0.48 ± 0.098
0.48CysSer: 0.48 ± 0.092
0.439CysThr: 0.439 ± 0.09
0.564CysVal: 0.564 ± 0.111
0.167CysTrp: 0.167 ± 0.065
0.606CysTyr: 0.606 ± 0.126
0.0CysXaa: 0.0 ± 0.0
Asp
3.739AspAla: 3.739 ± 0.317
0.689AspCys: 0.689 ± 0.126
2.862AspAsp: 2.862 ± 0.283
4.324AspGlu: 4.324 ± 0.329
3.008AspPhe: 3.008 ± 0.237
4.053AspGly: 4.053 ± 0.33
0.752AspHis: 0.752 ± 0.128
4.575AspIle: 4.575 ± 0.28
5.056AspLys: 5.056 ± 0.404
5.035AspLeu: 5.035 ± 0.348
1.817AspMet: 1.817 ± 0.184
3.531AspAsn: 3.531 ± 0.249
2.298AspPro: 2.298 ± 0.215
1.797AspGln: 1.797 ± 0.19
2.758AspArg: 2.758 ± 0.23
3.05AspSer: 3.05 ± 0.267
3.969AspThr: 3.969 ± 0.293
4.387AspVal: 4.387 ± 0.246
0.794AspTrp: 0.794 ± 0.146
3.217AspTyr: 3.217 ± 0.236
0.0AspXaa: 0.0 ± 0.0
Glu
5.181GluAla: 5.181 ± 0.349
0.543GluCys: 0.543 ± 0.117
4.847GluAsp: 4.847 ± 0.368
8.356GluGlu: 8.356 ± 0.747
2.34GluPhe: 2.34 ± 0.233
5.014GluGly: 5.014 ± 0.364
1.295GluHis: 1.295 ± 0.154
4.68GluIle: 4.68 ± 0.361
6.037GluLys: 6.037 ± 0.554
6.998GluLeu: 6.998 ± 0.418
2.361GluMet: 2.361 ± 0.233
3.28GluAsn: 3.28 ± 0.236
2.047GluPro: 2.047 ± 0.256
3.343GluGln: 3.343 ± 0.324
3.28GluArg: 3.28 ± 0.251
3.175GluSer: 3.175 ± 0.273
3.719GluThr: 3.719 ± 0.262
5.661GluVal: 5.661 ± 0.366
0.982GluTrp: 0.982 ± 0.144
3.155GluTyr: 3.155 ± 0.364
0.0GluXaa: 0.0 ± 0.0
Phe
2.006PheAla: 2.006 ± 0.167
0.397PheCys: 0.397 ± 0.082
2.82PheAsp: 2.82 ± 0.27
2.611PheGlu: 2.611 ± 0.217
1.337PhePhe: 1.337 ± 0.169
2.549PheGly: 2.549 ± 0.22
0.689PheHis: 0.689 ± 0.127
2.841PheIle: 2.841 ± 0.247
2.904PheLys: 2.904 ± 0.244
3.134PheLeu: 3.134 ± 0.249
0.982PheMet: 0.982 ± 0.152
2.382PheAsn: 2.382 ± 0.214
1.045PhePro: 1.045 ± 0.166
1.274PheGln: 1.274 ± 0.165
1.4PheArg: 1.4 ± 0.173
2.716PheSer: 2.716 ± 0.216
2.987PheThr: 2.987 ± 0.267
2.758PheVal: 2.758 ± 0.242
0.272PheTrp: 0.272 ± 0.077
1.671PheTyr: 1.671 ± 0.204
0.0PheXaa: 0.0 ± 0.0
Gly
3.948GlyAla: 3.948 ± 0.361
0.606GlyCys: 0.606 ± 0.124
4.136GlyAsp: 4.136 ± 0.305
4.366GlyGlu: 4.366 ± 0.294
2.57GlyPhe: 2.57 ± 0.262
5.223GlyGly: 5.223 ± 0.746
1.065GlyHis: 1.065 ± 0.153
3.551GlyIle: 3.551 ± 0.33
5.411GlyLys: 5.411 ± 0.345
5.745GlyLeu: 5.745 ± 0.347
1.692GlyMet: 1.692 ± 0.182
3.76GlyAsn: 3.76 ± 0.28
0.689GlyPro: 0.689 ± 0.099
2.152GlyGln: 2.152 ± 0.232
2.402GlyArg: 2.402 ± 0.21
4.721GlySer: 4.721 ± 0.344
4.888GlyThr: 4.888 ± 0.537
4.888GlyVal: 4.888 ± 0.249
0.94GlyTrp: 0.94 ± 0.143
3.551GlyTyr: 3.551 ± 0.299
0.0GlyXaa: 0.0 ± 0.0
His
1.003HisAla: 1.003 ± 0.138
0.167HisCys: 0.167 ± 0.063
1.065HisAsp: 1.065 ± 0.138
1.191HisGlu: 1.191 ± 0.155
0.773HisPhe: 0.773 ± 0.137
0.898HisGly: 0.898 ± 0.157
0.439HisHis: 0.439 ± 0.094
1.4HisIle: 1.4 ± 0.15
1.253HisLys: 1.253 ± 0.149
1.525HisLeu: 1.525 ± 0.187
0.46HisMet: 0.46 ± 0.109
1.191HisAsn: 1.191 ± 0.15
0.585HisPro: 0.585 ± 0.114
0.48HisGln: 0.48 ± 0.112
1.086HisArg: 1.086 ± 0.157
1.024HisSer: 1.024 ± 0.165
0.919HisThr: 0.919 ± 0.122
1.483HisVal: 1.483 ± 0.173
0.146HisTrp: 0.146 ± 0.051
0.919HisTyr: 0.919 ± 0.139
0.0HisXaa: 0.0 ± 0.0
Ile
4.45IleAla: 4.45 ± 0.293
0.48IleCys: 0.48 ± 0.123
5.014IleAsp: 5.014 ± 0.274
5.452IleGlu: 5.452 ± 0.359
2.068IlePhe: 2.068 ± 0.207
3.698IleGly: 3.698 ± 0.264
1.17IleHis: 1.17 ± 0.156
3.635IleIle: 3.635 ± 0.288
4.993IleLys: 4.993 ± 0.325
4.074IleLeu: 4.074 ± 0.346
1.253IleMet: 1.253 ± 0.165
3.614IleAsn: 3.614 ± 0.242
2.549IlePro: 2.549 ± 0.233
2.57IleGln: 2.57 ± 0.211
2.778IleArg: 2.778 ± 0.219
3.886IleSer: 3.886 ± 0.323
4.492IleThr: 4.492 ± 0.411
4.241IleVal: 4.241 ± 0.325
0.46IleTrp: 0.46 ± 0.107
1.985IleTyr: 1.985 ± 0.203
0.0IleXaa: 0.0 ± 0.0
Lys
5.118LysAla: 5.118 ± 0.368
0.648LysCys: 0.648 ± 0.123
4.909LysAsp: 4.909 ± 0.319
7.834LysGlu: 7.834 ± 0.579
2.799LysPhe: 2.799 ± 0.236
5.348LysGly: 5.348 ± 0.45
1.483LysHis: 1.483 ± 0.169
3.865LysIle: 3.865 ± 0.296
5.933LysLys: 5.933 ± 0.496
6.476LysLeu: 6.476 ± 0.349
2.59LysMet: 2.59 ± 0.256
3.426LysAsn: 3.426 ± 0.315
2.883LysPro: 2.883 ± 0.23
3.551LysGln: 3.551 ± 0.307
3.76LysArg: 3.76 ± 0.319
3.886LysSer: 3.886 ± 0.336
4.283LysThr: 4.283 ± 0.285
5.285LysVal: 5.285 ± 0.365
0.606LysTrp: 0.606 ± 0.1
2.987LysTyr: 2.987 ± 0.298
0.0LysXaa: 0.0 ± 0.0
Leu
5.808LeuAla: 5.808 ± 0.325
0.648LeuCys: 0.648 ± 0.129
5.724LeuAsp: 5.724 ± 0.398
6.121LeuGlu: 6.121 ± 0.45
3.175LeuPhe: 3.175 ± 0.285
5.181LeuGly: 5.181 ± 0.393
1.713LeuHis: 1.713 ± 0.191
4.93LeuIle: 4.93 ± 0.308
5.933LeuLys: 5.933 ± 0.321
5.87LeuLeu: 5.87 ± 0.453
1.964LeuMet: 1.964 ± 0.205
4.993LeuAsn: 4.993 ± 0.291
3.029LeuPro: 3.029 ± 0.267
3.322LeuGln: 3.322 ± 0.26
3.489LeuArg: 3.489 ± 0.296
5.264LeuSer: 5.264 ± 0.359
5.39LeuThr: 5.39 ± 0.328
5.202LeuVal: 5.202 ± 0.367
0.877LeuTrp: 0.877 ± 0.138
3.301LeuTyr: 3.301 ± 0.248
0.0LeuXaa: 0.0 ± 0.0
Met
2.006MetAla: 2.006 ± 0.186
0.146MetCys: 0.146 ± 0.05
1.274MetAsp: 1.274 ± 0.16
1.755MetGlu: 1.755 ± 0.224
1.233MetPhe: 1.233 ± 0.157
1.191MetGly: 1.191 ± 0.143
0.501MetHis: 0.501 ± 0.104
1.504MetIle: 1.504 ± 0.189
2.611MetLys: 2.611 ± 0.237
1.838MetLeu: 1.838 ± 0.211
0.543MetMet: 0.543 ± 0.107
1.379MetAsn: 1.379 ± 0.164
0.522MetPro: 0.522 ± 0.114
0.982MetGln: 0.982 ± 0.163
1.274MetArg: 1.274 ± 0.168
1.964MetSer: 1.964 ± 0.228
1.922MetThr: 1.922 ± 0.165
1.421MetVal: 1.421 ± 0.164
0.125MetTrp: 0.125 ± 0.05
1.295MetTyr: 1.295 ± 0.171
0.0MetXaa: 0.0 ± 0.0
Asn
3.092AsnAla: 3.092 ± 0.266
0.439AsnCys: 0.439 ± 0.105
2.737AsnAsp: 2.737 ± 0.21
3.134AsnGlu: 3.134 ± 0.292
2.152AsnPhe: 2.152 ± 0.18
4.366AsnGly: 4.366 ± 0.307
0.94AsnHis: 0.94 ± 0.119
3.363AsnIle: 3.363 ± 0.296
4.157AsnLys: 4.157 ± 0.284
4.199AsnLeu: 4.199 ± 0.315
1.567AsnMet: 1.567 ± 0.174
2.966AsnAsn: 2.966 ± 0.311
2.528AsnPro: 2.528 ± 0.251
1.817AsnGln: 1.817 ± 0.226
2.507AsnArg: 2.507 ± 0.249
3.572AsnSer: 3.572 ± 0.358
3.886AsnThr: 3.886 ± 0.27
3.447AsnVal: 3.447 ± 0.284
0.564AsnTrp: 0.564 ± 0.103
2.716AsnTyr: 2.716 ± 0.229
0.0AsnXaa: 0.0 ± 0.0
Pro
2.402ProAla: 2.402 ± 0.287
0.313ProCys: 0.313 ± 0.095
2.047ProAsp: 2.047 ± 0.203
2.507ProGlu: 2.507 ± 0.258
1.149ProPhe: 1.149 ± 0.182
1.504ProGly: 1.504 ± 0.187
0.585ProHis: 0.585 ± 0.108
2.194ProIle: 2.194 ± 0.201
2.778ProLys: 2.778 ± 0.233
2.59ProLeu: 2.59 ± 0.27
0.752ProMet: 0.752 ± 0.147
2.11ProAsn: 2.11 ± 0.213
1.128ProPro: 1.128 ± 0.304
0.857ProGln: 0.857 ± 0.139
1.065ProArg: 1.065 ± 0.144
2.006ProSer: 2.006 ± 0.207
2.799ProThr: 2.799 ± 0.32
2.57ProVal: 2.57 ± 0.216
0.292ProTrp: 0.292 ± 0.063
1.504ProTyr: 1.504 ± 0.186
0.0ProXaa: 0.0 ± 0.0
Gln
2.904GlnAla: 2.904 ± 0.275
0.146GlnCys: 0.146 ± 0.05
2.068GlnAsp: 2.068 ± 0.216
3.489GlnGlu: 3.489 ± 0.32
1.588GlnPhe: 1.588 ± 0.176
2.528GlnGly: 2.528 ± 0.242
1.045GlnHis: 1.045 ± 0.152
1.734GlnIle: 1.734 ± 0.196
2.361GlnLys: 2.361 ± 0.243
3.447GlnLeu: 3.447 ± 0.339
1.024GlnMet: 1.024 ± 0.172
1.546GlnAsn: 1.546 ± 0.199
1.316GlnPro: 1.316 ± 0.181
1.629GlnGln: 1.629 ± 0.241
1.734GlnArg: 1.734 ± 0.184
1.922GlnSer: 1.922 ± 0.263
2.11GlnThr: 2.11 ± 0.203
2.465GlnVal: 2.465 ± 0.216
0.522GlnTrp: 0.522 ± 0.118
1.734GlnTyr: 1.734 ± 0.183
0.0GlnXaa: 0.0 ± 0.0
Arg
2.298ArgAla: 2.298 ± 0.229
0.501ArgCys: 0.501 ± 0.138
2.737ArgAsp: 2.737 ± 0.219
3.51ArgGlu: 3.51 ± 0.293
1.901ArgPhe: 1.901 ± 0.193
2.862ArgGly: 2.862 ± 0.246
0.71ArgHis: 0.71 ± 0.132
3.05ArgIle: 3.05 ± 0.245
3.468ArgLys: 3.468 ± 0.29
4.074ArgLeu: 4.074 ± 0.288
1.274ArgMet: 1.274 ± 0.161
1.964ArgAsn: 1.964 ± 0.215
1.045ArgPro: 1.045 ± 0.159
1.588ArgGln: 1.588 ± 0.18
2.026ArgArg: 2.026 ± 0.251
2.068ArgSer: 2.068 ± 0.169
2.695ArgThr: 2.695 ± 0.23
2.82ArgVal: 2.82 ± 0.23
0.522ArgTrp: 0.522 ± 0.103
2.298ArgTyr: 2.298 ± 0.191
0.0ArgXaa: 0.0 ± 0.0
Ser
3.447SerAla: 3.447 ± 0.306
0.292SerCys: 0.292 ± 0.087
3.363SerAsp: 3.363 ± 0.257
3.823SerGlu: 3.823 ± 0.29
2.925SerPhe: 2.925 ± 0.224
4.053SerGly: 4.053 ± 0.357
0.857SerHis: 0.857 ± 0.126
3.948SerIle: 3.948 ± 0.302
4.366SerLys: 4.366 ± 0.335
5.097SerLeu: 5.097 ± 0.355
1.671SerMet: 1.671 ± 0.186
3.071SerAsn: 3.071 ± 0.236
2.006SerPro: 2.006 ± 0.221
1.985SerGln: 1.985 ± 0.211
2.57SerArg: 2.57 ± 0.214
4.053SerSer: 4.053 ± 0.335
3.802SerThr: 3.802 ± 0.37
4.178SerVal: 4.178 ± 0.336
0.71SerTrp: 0.71 ± 0.12
2.507SerTyr: 2.507 ± 0.212
0.0SerXaa: 0.0 ± 0.0
Thr
4.45ThrAla: 4.45 ± 0.441
0.46ThrCys: 0.46 ± 0.112
3.781ThrAsp: 3.781 ± 0.314
4.262ThrGlu: 4.262 ± 0.342
2.423ThrPhe: 2.423 ± 0.245
5.285ThrGly: 5.285 ± 0.451
1.212ThrHis: 1.212 ± 0.165
4.429ThrIle: 4.429 ± 0.347
4.366ThrLys: 4.366 ± 0.296
5.787ThrLeu: 5.787 ± 0.399
1.212ThrMet: 1.212 ± 0.147
3.719ThrAsn: 3.719 ± 0.311
3.05ThrPro: 3.05 ± 0.291
2.486ThrGln: 2.486 ± 0.254
2.549ThrArg: 2.549 ± 0.226
3.259ThrSer: 3.259 ± 0.286
4.554ThrThr: 4.554 ± 0.356
5.494ThrVal: 5.494 ± 0.425
0.71ThrTrp: 0.71 ± 0.099
3.238ThrTyr: 3.238 ± 0.334
0.0ThrXaa: 0.0 ± 0.0
Val
4.596ValAla: 4.596 ± 0.272
0.522ValCys: 0.522 ± 0.122
4.366ValAsp: 4.366 ± 0.249
4.972ValGlu: 4.972 ± 0.4
2.402ValPhe: 2.402 ± 0.22
3.781ValGly: 3.781 ± 0.288
1.546ValHis: 1.546 ± 0.149
4.638ValIle: 4.638 ± 0.302
5.808ValLys: 5.808 ± 0.38
5.682ValLeu: 5.682 ± 0.344
1.567ValMet: 1.567 ± 0.199
3.844ValAsn: 3.844 ± 0.283
2.716ValPro: 2.716 ± 0.23
2.716ValGln: 2.716 ± 0.303
3.029ValArg: 3.029 ± 0.245
4.095ValSer: 4.095 ± 0.293
5.014ValThr: 5.014 ± 0.396
4.492ValVal: 4.492 ± 0.347
0.627ValTrp: 0.627 ± 0.111
2.862ValTyr: 2.862 ± 0.252
0.0ValXaa: 0.0 ± 0.0
Trp
0.815TrpAla: 0.815 ± 0.108
0.146TrpCys: 0.146 ± 0.054
0.773TrpAsp: 0.773 ± 0.132
0.648TrpGlu: 0.648 ± 0.137
0.501TrpPhe: 0.501 ± 0.106
0.669TrpGly: 0.669 ± 0.148
0.167TrpHis: 0.167 ± 0.059
0.376TrpIle: 0.376 ± 0.078
0.919TrpLys: 0.919 ± 0.145
0.961TrpLeu: 0.961 ± 0.127
0.23TrpMet: 0.23 ± 0.07
0.522TrpAsn: 0.522 ± 0.12
0.084TrpPro: 0.084 ± 0.039
0.501TrpGln: 0.501 ± 0.112
0.376TrpArg: 0.376 ± 0.086
0.689TrpSer: 0.689 ± 0.15
0.501TrpThr: 0.501 ± 0.096
0.794TrpVal: 0.794 ± 0.135
0.167TrpTrp: 0.167 ± 0.06
0.648TrpTyr: 0.648 ± 0.114
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.528TyrAla: 2.528 ± 0.273
0.334TyrCys: 0.334 ± 0.085
2.695TyrAsp: 2.695 ± 0.279
3.28TyrGlu: 3.28 ± 0.296
1.546TyrPhe: 1.546 ± 0.203
2.966TyrGly: 2.966 ± 0.243
0.836TyrHis: 0.836 ± 0.135
2.862TyrIle: 2.862 ± 0.255
3.51TyrLys: 3.51 ± 0.291
3.51TyrLeu: 3.51 ± 0.279
1.045TyrMet: 1.045 ± 0.163
2.799TyrAsn: 2.799 ± 0.225
1.191TyrPro: 1.191 ± 0.153
1.713TyrGln: 1.713 ± 0.186
2.361TyrArg: 2.361 ± 0.222
2.737TyrSer: 2.737 ± 0.286
3.572TyrThr: 3.572 ± 0.338
3.343TyrVal: 3.343 ± 0.251
0.355TyrTrp: 0.355 ± 0.084
1.483TyrTyr: 1.483 ± 0.165
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 231 proteins (47869 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski