Roseovarius halotolerans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseovarius

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3635 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X6ZIT9|A0A1X6ZIT9_9RHOB Uncharacterized protein OS=Roseovarius halotolerans OX=505353 GN=ROH8110_02901 PE=4 SV=1
MM1 pKa = 7.64LKK3 pKa = 9.78QGSMIAATIAMATGPAFAGSASAPAPDD30 pKa = 4.1PVVSAPAPAPAPAPASPDD48 pKa = 2.83WTGGYY53 pKa = 10.08VGGQLGYY60 pKa = 11.61GNVDD64 pKa = 3.32TNAPGVDD71 pKa = 3.32GDD73 pKa = 4.21GAIGGLVMGYY83 pKa = 10.41DD84 pKa = 3.67YY85 pKa = 11.74DD86 pKa = 4.03FGQWVAGAGLDD97 pKa = 4.48YY98 pKa = 11.11DD99 pKa = 3.82WADD102 pKa = 3.07IGLGAGNPTIEE113 pKa = 3.95NVLRR117 pKa = 11.84VKK119 pKa = 10.76LRR121 pKa = 11.84GGYY124 pKa = 10.08KK125 pKa = 9.57IGNGLLYY132 pKa = 9.53GTGGWAQADD141 pKa = 4.14TDD143 pKa = 4.3TLGDD147 pKa = 3.77DD148 pKa = 3.7DD149 pKa = 6.19GYY151 pKa = 11.31FIGAGYY157 pKa = 10.42EE158 pKa = 4.05HH159 pKa = 7.66MITQNFAMGGEE170 pKa = 4.23VLYY173 pKa = 11.16HH174 pKa = 6.42EE175 pKa = 5.26FDD177 pKa = 3.83NFNSTNTDD185 pKa = 2.95VEE187 pKa = 4.31ATTVQVRR194 pKa = 11.84GTFRR198 pKa = 11.84FF199 pKa = 3.6

Molecular weight: 20.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X6YQB9|A0A1X6YQB9_9RHOB Uridylate kinase OS=Roseovarius halotolerans OX=505353 GN=pyrH PE=3 SV=1
MM1 pKa = 6.98NANRR5 pKa = 11.84IVTMIIRR12 pKa = 11.84MVMRR16 pKa = 11.84QLMSRR21 pKa = 11.84GVNAGISAMGNRR33 pKa = 11.84KK34 pKa = 9.34GKK36 pKa = 9.87SQGQGQTQRR45 pKa = 11.84GQQQAGQGQGPDD57 pKa = 3.22TRR59 pKa = 11.84KK60 pKa = 6.94TQQRR64 pKa = 11.84ARR66 pKa = 11.84QAMRR70 pKa = 11.84LGRR73 pKa = 11.84RR74 pKa = 11.84FGKK77 pKa = 10.4FF78 pKa = 2.76

Molecular weight: 8.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3635

0

3635

1122804

30

1750

308.9

33.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.358 ± 0.056

0.932 ± 0.013

6.12 ± 0.037

6.097 ± 0.044

3.644 ± 0.029

8.826 ± 0.04

2.122 ± 0.021

5.184 ± 0.025

2.946 ± 0.035

10.121 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.894 ± 0.019

2.452 ± 0.021

5.111 ± 0.027

3.084 ± 0.022

7.103 ± 0.043

5.144 ± 0.025

5.169 ± 0.024

7.105 ± 0.03

1.397 ± 0.017

2.191 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski