Mycobacterium Phage Niklas

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482JG54|A0A482JG54_9CAUD Uncharacterized protein OS=Mycobacterium Phage Niklas OX=2517936 GN=16 PE=4 SV=1
MM1 pKa = 7.84RR2 pKa = 11.84APATAEE8 pKa = 4.38VPCPACGEE16 pKa = 4.67PITLALGFALDD27 pKa = 4.06EE28 pKa = 4.64PEE30 pKa = 5.18PGASSAPCRR39 pKa = 11.84VIPLDD44 pKa = 3.12ITEE47 pKa = 4.51RR48 pKa = 11.84AQEE51 pKa = 4.17HH52 pKa = 6.63GEE54 pKa = 4.13VCPVLTGGGCDD65 pKa = 3.48GG66 pKa = 4.47

Molecular weight: 6.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482JCQ3|A0A482JCQ3_9CAUD Head-to-tail stopper OS=Mycobacterium Phage Niklas OX=2517936 GN=15 PE=4 SV=1
MM1 pKa = 7.85SEE3 pKa = 3.73GRR5 pKa = 11.84NTARR9 pKa = 11.84RR10 pKa = 11.84NRR12 pKa = 11.84FRR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 3.63WLRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84EE22 pKa = 3.97DD23 pKa = 3.76CAVCGAPIDD32 pKa = 3.9YY33 pKa = 8.44DD34 pKa = 3.74APHH37 pKa = 7.08DD38 pKa = 4.41HH39 pKa = 7.14PLSFQVDD46 pKa = 4.83HH47 pKa = 6.55ITPLARR53 pKa = 11.84GGTDD57 pKa = 2.93TLDD60 pKa = 3.25NTQPVHH66 pKa = 6.63RR67 pKa = 11.84KK68 pKa = 9.31CNRR71 pKa = 11.84DD72 pKa = 3.21KK73 pKa = 11.19SDD75 pKa = 4.3KK76 pKa = 10.16LPEE79 pKa = 4.51MISGAVRR86 pKa = 11.84FVTHH90 pKa = 6.96RR91 pKa = 11.84MWRR94 pKa = 11.84PP95 pKa = 2.81

Molecular weight: 11.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

18901

39

1384

194.9

21.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.645 ± 0.549

1.069 ± 0.13

6.672 ± 0.301

5.793 ± 0.345

2.603 ± 0.138

8.709 ± 0.455

2.233 ± 0.171

3.809 ± 0.155

2.968 ± 0.191

8.312 ± 0.198

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.286 ± 0.102

2.439 ± 0.167

5.809 ± 0.261

3.227 ± 0.131

7.534 ± 0.323

4.883 ± 0.191

5.73 ± 0.197

7.862 ± 0.262

2.085 ± 0.102

2.333 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski