Amino acid dipepetide frequency for Mycobacterium Phage Niklas

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.857AlaAla: 22.857 ± 1.835
1.27AlaCys: 1.27 ± 0.273
9.788AlaAsp: 9.788 ± 0.794
10.688AlaGlu: 10.688 ± 1.139
3.069AlaPhe: 3.069 ± 0.567
8.519AlaGly: 8.519 ± 0.797
2.857AlaHis: 2.857 ± 0.392
5.185AlaIle: 5.185 ± 0.539
3.598AlaLys: 3.598 ± 0.524
12.857AlaLeu: 12.857 ± 0.962
4.021AlaMet: 4.021 ± 0.518
2.434AlaAsn: 2.434 ± 0.374
8.36AlaPro: 8.36 ± 0.77
4.921AlaGln: 4.921 ± 0.649
8.519AlaArg: 8.519 ± 0.685
5.714AlaSer: 5.714 ± 0.631
6.455AlaThr: 6.455 ± 0.814
10.423AlaVal: 10.423 ± 0.971
2.275AlaTrp: 2.275 ± 0.316
2.646AlaTyr: 2.646 ± 0.366
0.0AlaXaa: 0.0 ± 0.0
Cys
1.27CysAla: 1.27 ± 0.282
0.159CysCys: 0.159 ± 0.096
0.635CysAsp: 0.635 ± 0.218
0.794CysGlu: 0.794 ± 0.231
0.159CysPhe: 0.159 ± 0.086
1.27CysGly: 1.27 ± 0.321
0.212CysHis: 0.212 ± 0.102
0.265CysIle: 0.265 ± 0.099
0.476CysLys: 0.476 ± 0.172
0.529CysLeu: 0.529 ± 0.172
0.159CysMet: 0.159 ± 0.1
0.317CysAsn: 0.317 ± 0.118
1.164CysPro: 1.164 ± 0.28
0.265CysGln: 0.265 ± 0.118
0.582CysArg: 0.582 ± 0.182
0.794CysSer: 0.794 ± 0.198
0.423CysThr: 0.423 ± 0.145
0.741CysVal: 0.741 ± 0.196
0.423CysTrp: 0.423 ± 0.149
0.053CysTyr: 0.053 ± 0.045
0.0CysXaa: 0.0 ± 0.0
Asp
9.524AspAla: 9.524 ± 0.837
0.688AspCys: 0.688 ± 0.209
5.926AspAsp: 5.926 ± 0.673
6.19AspGlu: 6.19 ± 0.669
1.164AspPhe: 1.164 ± 0.242
6.455AspGly: 6.455 ± 0.495
1.217AspHis: 1.217 ± 0.245
1.64AspIle: 1.64 ± 0.309
1.905AspLys: 1.905 ± 0.327
5.503AspLeu: 5.503 ± 0.632
1.746AspMet: 1.746 ± 0.393
1.429AspAsn: 1.429 ± 0.228
3.757AspPro: 3.757 ± 0.434
2.54AspGln: 2.54 ± 0.386
5.397AspArg: 5.397 ± 0.633
2.698AspSer: 2.698 ± 0.371
3.016AspThr: 3.016 ± 0.424
3.651AspVal: 3.651 ± 0.291
0.952AspTrp: 0.952 ± 0.213
1.323AspTyr: 1.323 ± 0.237
0.0AspXaa: 0.0 ± 0.0
Glu
7.831GluAla: 7.831 ± 0.936
0.741GluCys: 0.741 ± 0.189
3.016GluAsp: 3.016 ± 0.473
0.741GluGlu: 0.741 ± 0.182
1.958GluPhe: 1.958 ± 0.313
4.603GluGly: 4.603 ± 0.509
1.905GluHis: 1.905 ± 0.336
2.54GluIle: 2.54 ± 0.353
1.746GluLys: 1.746 ± 0.381
6.085GluLeu: 6.085 ± 0.6
1.799GluMet: 1.799 ± 0.292
1.164GluAsn: 1.164 ± 0.265
2.646GluPro: 2.646 ± 0.412
2.381GluGln: 2.381 ± 0.411
5.079GluArg: 5.079 ± 0.56
2.434GluSer: 2.434 ± 0.322
2.698GluThr: 2.698 ± 0.361
5.556GluVal: 5.556 ± 0.679
0.899GluTrp: 0.899 ± 0.274
2.116GluTyr: 2.116 ± 0.448
0.0GluXaa: 0.0 ± 0.0
Phe
3.069PheAla: 3.069 ± 0.517
0.317PheCys: 0.317 ± 0.147
3.175PheAsp: 3.175 ± 0.386
1.27PheGlu: 1.27 ± 0.237
0.635PhePhe: 0.635 ± 0.179
2.751PheGly: 2.751 ± 0.452
0.317PheHis: 0.317 ± 0.133
0.952PheIle: 0.952 ± 0.195
0.899PheLys: 0.899 ± 0.207
1.799PheLeu: 1.799 ± 0.291
0.529PheMet: 0.529 ± 0.157
1.058PheAsn: 1.058 ± 0.304
1.058PhePro: 1.058 ± 0.315
0.794PheGln: 0.794 ± 0.223
1.429PheArg: 1.429 ± 0.253
1.217PheSer: 1.217 ± 0.239
1.64PheThr: 1.64 ± 0.304
2.169PheVal: 2.169 ± 0.299
0.423PheTrp: 0.423 ± 0.158
0.529PheTyr: 0.529 ± 0.205
0.0PheXaa: 0.0 ± 0.0
Gly
11.058GlyAla: 11.058 ± 1.061
0.952GlyCys: 0.952 ± 0.221
4.921GlyAsp: 4.921 ± 0.569
5.079GlyGlu: 5.079 ± 0.471
2.275GlyPhe: 2.275 ± 0.437
8.889GlyGly: 8.889 ± 1.265
1.587GlyHis: 1.587 ± 0.337
2.275GlyIle: 2.275 ± 0.515
3.598GlyLys: 3.598 ± 0.553
7.46GlyLeu: 7.46 ± 0.721
1.958GlyMet: 1.958 ± 0.387
2.434GlyAsn: 2.434 ± 0.324
3.757GlyPro: 3.757 ± 0.553
2.487GlyGln: 2.487 ± 0.541
6.085GlyArg: 6.085 ± 0.614
5.344GlySer: 5.344 ± 0.615
5.132GlyThr: 5.132 ± 0.436
7.037GlyVal: 7.037 ± 0.597
2.434GlyTrp: 2.434 ± 0.308
2.328GlyTyr: 2.328 ± 0.394
0.0GlyXaa: 0.0 ± 0.0
His
2.169HisAla: 2.169 ± 0.356
0.265HisCys: 0.265 ± 0.129
1.323HisAsp: 1.323 ± 0.323
1.323HisGlu: 1.323 ± 0.316
0.741HisPhe: 0.741 ± 0.212
2.222HisGly: 2.222 ± 0.36
0.635HisHis: 0.635 ± 0.206
0.794HisIle: 0.794 ± 0.21
0.423HisLys: 0.423 ± 0.136
1.905HisLeu: 1.905 ± 0.287
0.476HisMet: 0.476 ± 0.16
0.688HisAsn: 0.688 ± 0.215
1.481HisPro: 1.481 ± 0.278
0.317HisGln: 0.317 ± 0.123
2.011HisArg: 2.011 ± 0.368
0.899HisSer: 0.899 ± 0.206
1.27HisThr: 1.27 ± 0.228
2.275HisVal: 2.275 ± 0.412
0.635HisTrp: 0.635 ± 0.184
0.476HisTyr: 0.476 ± 0.16
0.0HisXaa: 0.0 ± 0.0
Ile
5.82IleAla: 5.82 ± 0.66
0.053IleCys: 0.053 ± 0.062
3.333IleAsp: 3.333 ± 0.598
3.016IleGlu: 3.016 ± 0.467
0.741IlePhe: 0.741 ± 0.241
3.704IleGly: 3.704 ± 0.759
0.529IleHis: 0.529 ± 0.176
0.582IleIle: 0.582 ± 0.141
1.164IleLys: 1.164 ± 0.248
2.381IleLeu: 2.381 ± 0.356
0.476IleMet: 0.476 ± 0.153
1.217IleAsn: 1.217 ± 0.261
2.275IlePro: 2.275 ± 0.489
0.899IleGln: 0.899 ± 0.184
2.593IleArg: 2.593 ± 0.363
1.587IleSer: 1.587 ± 0.261
2.646IleThr: 2.646 ± 0.331
3.862IleVal: 3.862 ± 0.534
0.688IleTrp: 0.688 ± 0.204
0.529IleTyr: 0.529 ± 0.173
0.0IleXaa: 0.0 ± 0.0
Lys
3.598LysAla: 3.598 ± 0.483
0.423LysCys: 0.423 ± 0.142
1.217LysAsp: 1.217 ± 0.232
0.476LysGlu: 0.476 ± 0.152
0.847LysPhe: 0.847 ± 0.218
2.804LysGly: 2.804 ± 0.484
0.688LysHis: 0.688 ± 0.194
1.534LysIle: 1.534 ± 0.326
0.688LysLys: 0.688 ± 0.231
2.804LysLeu: 2.804 ± 0.359
0.741LysMet: 0.741 ± 0.21
0.476LysAsn: 0.476 ± 0.176
2.646LysPro: 2.646 ± 0.439
0.688LysGln: 0.688 ± 0.188
3.069LysArg: 3.069 ± 0.425
1.111LysSer: 1.111 ± 0.236
1.64LysThr: 1.64 ± 0.358
2.646LysVal: 2.646 ± 0.427
0.582LysTrp: 0.582 ± 0.176
1.005LysTyr: 1.005 ± 0.266
0.0LysXaa: 0.0 ± 0.0
Leu
12.857LeuAla: 12.857 ± 0.761
0.688LeuCys: 0.688 ± 0.209
8.413LeuAsp: 8.413 ± 0.709
2.54LeuGlu: 2.54 ± 0.329
2.011LeuPhe: 2.011 ± 0.322
7.143LeuGly: 7.143 ± 0.68
1.481LeuHis: 1.481 ± 0.29
3.757LeuIle: 3.757 ± 0.451
2.381LeuLys: 2.381 ± 0.285
5.608LeuLeu: 5.608 ± 0.575
1.587LeuMet: 1.587 ± 0.245
2.116LeuAsn: 2.116 ± 0.289
4.233LeuPro: 4.233 ± 0.605
2.434LeuGln: 2.434 ± 0.413
6.772LeuArg: 6.772 ± 0.598
4.868LeuSer: 4.868 ± 0.482
5.556LeuThr: 5.556 ± 0.501
5.767LeuVal: 5.767 ± 0.446
1.481LeuTrp: 1.481 ± 0.253
1.429LeuTyr: 1.429 ± 0.286
0.0LeuXaa: 0.0 ± 0.0
Met
3.069MetAla: 3.069 ± 0.433
0.159MetCys: 0.159 ± 0.103
0.529MetAsp: 0.529 ± 0.183
0.741MetGlu: 0.741 ± 0.232
0.688MetPhe: 0.688 ± 0.217
1.852MetGly: 1.852 ± 0.403
0.635MetHis: 0.635 ± 0.165
1.323MetIle: 1.323 ± 0.277
0.582MetLys: 0.582 ± 0.186
1.376MetLeu: 1.376 ± 0.282
0.265MetMet: 0.265 ± 0.124
0.688MetAsn: 0.688 ± 0.194
1.376MetPro: 1.376 ± 0.236
0.847MetGln: 0.847 ± 0.24
1.693MetArg: 1.693 ± 0.328
2.169MetSer: 2.169 ± 0.313
2.328MetThr: 2.328 ± 0.403
1.852MetVal: 1.852 ± 0.363
0.265MetTrp: 0.265 ± 0.112
0.423MetTyr: 0.423 ± 0.155
0.0MetXaa: 0.0 ± 0.0
Asn
2.963AsnAla: 2.963 ± 0.423
0.265AsnCys: 0.265 ± 0.115
1.429AsnAsp: 1.429 ± 0.248
1.164AsnGlu: 1.164 ± 0.26
0.688AsnPhe: 0.688 ± 0.191
3.545AsnGly: 3.545 ± 0.49
0.476AsnHis: 0.476 ± 0.166
0.635AsnIle: 0.635 ± 0.173
0.741AsnLys: 0.741 ± 0.193
2.116AsnLeu: 2.116 ± 0.391
0.423AsnMet: 0.423 ± 0.146
0.476AsnAsn: 0.476 ± 0.16
2.381AsnPro: 2.381 ± 0.341
0.423AsnGln: 0.423 ± 0.141
1.376AsnArg: 1.376 ± 0.223
1.005AsnSer: 1.005 ± 0.265
1.534AsnThr: 1.534 ± 0.252
1.905AsnVal: 1.905 ± 0.379
0.317AsnTrp: 0.317 ± 0.126
0.529AsnTyr: 0.529 ± 0.142
0.0AsnXaa: 0.0 ± 0.0
Pro
8.783ProAla: 8.783 ± 0.782
0.688ProCys: 0.688 ± 0.195
3.122ProAsp: 3.122 ± 0.398
4.656ProGlu: 4.656 ± 0.557
1.693ProPhe: 1.693 ± 0.26
5.926ProGly: 5.926 ± 0.451
1.164ProHis: 1.164 ± 0.314
2.011ProIle: 2.011 ± 0.338
1.693ProLys: 1.693 ± 0.273
4.127ProLeu: 4.127 ± 0.451
0.952ProMet: 0.952 ± 0.205
1.005ProAsn: 1.005 ± 0.333
2.963ProPro: 2.963 ± 0.446
1.693ProGln: 1.693 ± 0.379
3.545ProArg: 3.545 ± 0.56
2.804ProSer: 2.804 ± 0.41
3.122ProThr: 3.122 ± 0.407
5.556ProVal: 5.556 ± 0.636
1.058ProTrp: 1.058 ± 0.236
1.534ProTyr: 1.534 ± 0.268
0.0ProXaa: 0.0 ± 0.0
Gln
4.656GlnAla: 4.656 ± 0.497
0.212GlnCys: 0.212 ± 0.102
0.952GlnAsp: 0.952 ± 0.222
1.111GlnGlu: 1.111 ± 0.233
0.635GlnPhe: 0.635 ± 0.177
2.381GlnGly: 2.381 ± 0.365
1.217GlnHis: 1.217 ± 0.249
1.746GlnIle: 1.746 ± 0.27
0.899GlnLys: 0.899 ± 0.198
2.804GlnLeu: 2.804 ± 0.346
0.952GlnMet: 0.952 ± 0.198
0.529GlnAsn: 0.529 ± 0.177
2.275GlnPro: 2.275 ± 0.383
1.64GlnGln: 1.64 ± 0.314
2.54GlnArg: 2.54 ± 0.314
1.058GlnSer: 1.058 ± 0.246
1.746GlnThr: 1.746 ± 0.294
2.698GlnVal: 2.698 ± 0.356
0.794GlnTrp: 0.794 ± 0.208
1.429GlnTyr: 1.429 ± 0.263
0.0GlnXaa: 0.0 ± 0.0
Arg
7.354ArgAla: 7.354 ± 0.656
1.058ArgCys: 1.058 ± 0.274
4.233ArgAsp: 4.233 ± 0.394
5.079ArgGlu: 5.079 ± 0.549
2.698ArgPhe: 2.698 ± 0.363
5.344ArgGly: 5.344 ± 0.472
2.011ArgHis: 2.011 ± 0.442
3.545ArgIle: 3.545 ± 0.569
3.069ArgLys: 3.069 ± 0.447
6.19ArgLeu: 6.19 ± 0.619
2.487ArgMet: 2.487 ± 0.382
2.011ArgAsn: 2.011 ± 0.34
3.598ArgPro: 3.598 ± 0.544
3.016ArgGln: 3.016 ± 0.33
6.561ArgArg: 6.561 ± 0.817
3.598ArgSer: 3.598 ± 0.441
4.392ArgThr: 4.392 ± 0.521
4.868ArgVal: 4.868 ± 0.518
1.958ArgTrp: 1.958 ± 0.318
2.222ArgTyr: 2.222 ± 0.385
0.0ArgXaa: 0.0 ± 0.0
Ser
6.614SerAla: 6.614 ± 0.645
0.423SerCys: 0.423 ± 0.135
3.122SerAsp: 3.122 ± 0.358
2.434SerGlu: 2.434 ± 0.402
1.429SerPhe: 1.429 ± 0.261
4.762SerGly: 4.762 ± 0.518
1.164SerHis: 1.164 ± 0.238
2.222SerIle: 2.222 ± 0.269
1.164SerLys: 1.164 ± 0.2
3.651SerLeu: 3.651 ± 0.385
1.429SerMet: 1.429 ± 0.294
1.534SerAsn: 1.534 ± 0.267
3.228SerPro: 3.228 ± 0.401
1.323SerGln: 1.323 ± 0.232
3.016SerArg: 3.016 ± 0.372
2.963SerSer: 2.963 ± 0.419
3.439SerThr: 3.439 ± 0.546
3.228SerVal: 3.228 ± 0.468
0.794SerTrp: 0.794 ± 0.174
0.899SerTyr: 0.899 ± 0.185
0.0SerXaa: 0.0 ± 0.0
Thr
7.725ThrAla: 7.725 ± 0.714
0.582ThrCys: 0.582 ± 0.136
3.333ThrAsp: 3.333 ± 0.465
3.28ThrGlu: 3.28 ± 0.366
2.169ThrPhe: 2.169 ± 0.382
4.868ThrGly: 4.868 ± 0.45
1.429ThrHis: 1.429 ± 0.336
2.963ThrIle: 2.963 ± 0.436
1.217ThrLys: 1.217 ± 0.251
4.286ThrLeu: 4.286 ± 0.523
0.635ThrMet: 0.635 ± 0.155
1.27ThrAsn: 1.27 ± 0.241
4.021ThrPro: 4.021 ± 0.465
1.746ThrGln: 1.746 ± 0.343
3.862ThrArg: 3.862 ± 0.45
3.069ThrSer: 3.069 ± 0.455
3.122ThrThr: 3.122 ± 0.446
4.921ThrVal: 4.921 ± 0.51
1.64ThrTrp: 1.64 ± 0.242
1.164ThrTyr: 1.164 ± 0.232
0.0ThrXaa: 0.0 ± 0.0
Val
10.37ValAla: 10.37 ± 0.877
1.058ValCys: 1.058 ± 0.259
5.344ValAsp: 5.344 ± 0.606
5.238ValGlu: 5.238 ± 0.553
1.64ValPhe: 1.64 ± 0.328
6.138ValGly: 6.138 ± 0.588
2.063ValHis: 2.063 ± 0.458
2.804ValIle: 2.804 ± 0.386
2.646ValLys: 2.646 ± 0.368
6.878ValLeu: 6.878 ± 0.599
1.376ValMet: 1.376 ± 0.224
2.063ValAsn: 2.063 ± 0.363
5.608ValPro: 5.608 ± 0.702
1.429ValGln: 1.429 ± 0.262
6.296ValArg: 6.296 ± 0.668
3.333ValSer: 3.333 ± 0.377
4.497ValThr: 4.497 ± 0.474
6.085ValVal: 6.085 ± 0.832
2.275ValTrp: 2.275 ± 0.31
1.481ValTyr: 1.481 ± 0.256
0.0ValXaa: 0.0 ± 0.0
Trp
2.328TrpAla: 2.328 ± 0.386
0.317TrpCys: 0.317 ± 0.119
0.688TrpAsp: 0.688 ± 0.168
0.741TrpGlu: 0.741 ± 0.206
0.476TrpPhe: 0.476 ± 0.173
1.64TrpGly: 1.64 ± 0.259
0.423TrpHis: 0.423 ± 0.16
0.952TrpIle: 0.952 ± 0.192
0.159TrpLys: 0.159 ± 0.093
2.91TrpLeu: 2.91 ± 0.399
0.423TrpMet: 0.423 ± 0.167
0.794TrpAsn: 0.794 ± 0.234
0.529TrpPro: 0.529 ± 0.161
1.481TrpGln: 1.481 ± 0.253
2.857TrpArg: 2.857 ± 0.394
0.899TrpSer: 0.899 ± 0.241
0.899TrpThr: 0.899 ± 0.202
1.323TrpVal: 1.323 ± 0.255
0.423TrpTrp: 0.423 ± 0.139
0.582TrpTyr: 0.582 ± 0.156
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.54TyrAla: 2.54 ± 0.302
0.37TyrCys: 0.37 ± 0.137
2.222TyrAsp: 2.222 ± 0.374
1.376TyrGlu: 1.376 ± 0.252
0.317TyrPhe: 0.317 ± 0.127
2.275TyrGly: 2.275 ± 0.35
0.317TyrHis: 0.317 ± 0.121
0.37TyrIle: 0.37 ± 0.119
0.635TyrLys: 0.635 ± 0.2
1.852TyrLeu: 1.852 ± 0.401
0.37TyrMet: 0.37 ± 0.117
0.688TyrAsn: 0.688 ± 0.193
0.741TyrPro: 0.741 ± 0.165
0.952TyrGln: 0.952 ± 0.272
2.063TyrArg: 2.063 ± 0.346
1.27TyrSer: 1.27 ± 0.253
1.746TyrThr: 1.746 ± 0.315
2.063TyrVal: 2.063 ± 0.347
0.529TyrTrp: 0.529 ± 0.175
0.635TyrTyr: 0.635 ± 0.207
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 97 proteins (18901 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski