Arthrobacter sp. PAMC25564

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Arthrobacter; unclassified Arthrobacter

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3600 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4P7STE6|A0A4P7STE6_9MICC Endoribonuclease YbeY OS=Arthrobacter sp. PAMC25564 OX=2565366 GN=ybeY PE=3 SV=1
MM1 pKa = 7.35TMRR4 pKa = 11.84TGFTEE9 pKa = 6.3GEE11 pKa = 4.51VCWTDD16 pKa = 3.67LQTGDD21 pKa = 3.35VDD23 pKa = 3.72AAKK26 pKa = 10.57AFYY29 pKa = 10.35AAVFGWRR36 pKa = 11.84YY37 pKa = 9.9EE38 pKa = 4.1DD39 pKa = 5.31LPTPDD44 pKa = 3.16GRR46 pKa = 11.84SYY48 pKa = 11.58AQAFLGDD55 pKa = 3.72GLVTVIAPQPPQQEE69 pKa = 4.01AAAGRR74 pKa = 11.84WNVYY78 pKa = 9.39FATDD82 pKa = 3.7DD83 pKa = 3.73ARR85 pKa = 11.84EE86 pKa = 3.94LAAEE90 pKa = 4.51LAHH93 pKa = 6.06STGTLEE99 pKa = 4.78SGPEE103 pKa = 4.11SVGGAGVMVFFAPPGGGSTGAWQAGSHH130 pKa = 6.5FGAARR135 pKa = 11.84IQEE138 pKa = 4.31PGALAWAEE146 pKa = 4.13LLTPEE151 pKa = 4.16PQAAVGCFQQLFGHH165 pKa = 6.44EE166 pKa = 4.27VTEE169 pKa = 4.25YY170 pKa = 10.09PQDD173 pKa = 3.98DD174 pKa = 3.98GGTYY178 pKa = 7.37TTLTVNGTEE187 pKa = 4.33VAGIAPLPADD197 pKa = 4.88DD198 pKa = 5.06GGTRR202 pKa = 11.84QAGWQVYY209 pKa = 9.43FGVPNVADD217 pKa = 3.48AVEE220 pKa = 4.08AAVAAGAVVLVAPEE234 pKa = 4.06DD235 pKa = 3.81AGDD238 pKa = 4.0AGALATLQDD247 pKa = 3.67PQGGVFSLVEE257 pKa = 3.92VV258 pKa = 3.9

Molecular weight:
26.56 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4P7STK5|A0A4P7STK5_9MICC GntR family transcriptional regulator OS=Arthrobacter sp. PAMC25564 OX=2565366 GN=E5206_12460 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight:
4.08 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3600

0

3600

1191758

32

1728

331.0

35.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.273 ± 0.063

0.624 ± 0.009

5.511 ± 0.032

5.481 ± 0.039

3.259 ± 0.023

9.313 ± 0.032

2.093 ± 0.018

4.47 ± 0.028

2.791 ± 0.03

10.306 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.993 ± 0.016

2.422 ± 0.022

5.485 ± 0.031

3.063 ± 0.021

6.624 ± 0.036

5.804 ± 0.029

5.759 ± 0.032

8.24 ± 0.032

1.402 ± 0.017

2.086 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski