Amino acid dipepetide frequency for Arthrobacter sp. PAMC25564

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.697AlaAla: 21.697 ± 0.234
0.8AlaCys: 0.8 ± 0.028
7.115AlaAsp: 7.115 ± 0.095
7.99AlaGlu: 7.99 ± 0.107
3.748AlaPhe: 3.748 ± 0.057
14.193AlaGly: 14.193 ± 0.139
2.243AlaHis: 2.243 ± 0.038
5.069AlaIle: 5.069 ± 0.08
3.68AlaLys: 3.68 ± 0.069
13.015AlaLeu: 13.015 ± 0.124
2.767AlaMet: 2.767 ± 0.047
2.657AlaAsn: 2.657 ± 0.049
6.427AlaPro: 6.427 ± 0.109
3.82AlaGln: 3.82 ± 0.056
8.115AlaArg: 8.115 ± 0.095
7.055AlaSer: 7.055 ± 0.092
6.717AlaThr: 6.717 ± 0.082
11.686AlaVal: 11.686 ± 0.12
1.634AlaTrp: 1.634 ± 0.038
2.304AlaTyr: 2.304 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
0.726CysAla: 0.726 ± 0.025
0.067CysCys: 0.067 ± 0.009
0.36CysAsp: 0.36 ± 0.019
0.313CysGlu: 0.313 ± 0.017
0.198CysPhe: 0.198 ± 0.012
0.735CysGly: 0.735 ± 0.024
0.148CysHis: 0.148 ± 0.01
0.26CysIle: 0.26 ± 0.014
0.124CysLys: 0.124 ± 0.01
0.587CysLeu: 0.587 ± 0.021
0.107CysMet: 0.107 ± 0.009
0.149CysAsn: 0.149 ± 0.012
0.368CysPro: 0.368 ± 0.019
0.159CysGln: 0.159 ± 0.011
0.46CysArg: 0.46 ± 0.019
0.395CysSer: 0.395 ± 0.019
0.384CysThr: 0.384 ± 0.019
0.459CysVal: 0.459 ± 0.023
0.099CysTrp: 0.099 ± 0.01
0.141CysTyr: 0.141 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
7.436AspAla: 7.436 ± 0.087
0.325AspCys: 0.325 ± 0.017
2.906AspAsp: 2.906 ± 0.059
3.183AspGlu: 3.183 ± 0.056
1.969AspPhe: 1.969 ± 0.047
5.532AspGly: 5.532 ± 0.077
1.193AspHis: 1.193 ± 0.034
2.496AspIle: 2.496 ± 0.053
1.449AspLys: 1.449 ± 0.045
5.286AspLeu: 5.286 ± 0.072
0.895AspMet: 0.895 ± 0.026
1.103AspAsn: 1.103 ± 0.034
3.736AspPro: 3.736 ± 0.061
1.601AspGln: 1.601 ± 0.033
3.526AspArg: 3.526 ± 0.06
2.772AspSer: 2.772 ± 0.048
2.716AspThr: 2.716 ± 0.06
4.84AspVal: 4.84 ± 0.078
0.87AspTrp: 0.87 ± 0.026
1.275AspTyr: 1.275 ± 0.033
0.0AspXaa: 0.0 ± 0.0
Glu
7.004GluAla: 7.004 ± 0.097
0.31GluCys: 0.31 ± 0.016
2.885GluAsp: 2.885 ± 0.049
3.215GluGlu: 3.215 ± 0.063
1.794GluPhe: 1.794 ± 0.048
3.929GluGly: 3.929 ± 0.066
1.439GluHis: 1.439 ± 0.03
2.635GluIle: 2.635 ± 0.053
1.655GluLys: 1.655 ± 0.04
7.012GluLeu: 7.012 ± 0.091
0.961GluMet: 0.961 ± 0.03
1.438GluAsn: 1.438 ± 0.035
2.722GluPro: 2.722 ± 0.056
2.145GluGln: 2.145 ± 0.044
3.983GluArg: 3.983 ± 0.072
2.85GluSer: 2.85 ± 0.051
2.957GluThr: 2.957 ± 0.055
4.029GluVal: 4.029 ± 0.067
0.743GluTrp: 0.743 ± 0.026
1.103GluTyr: 1.103 ± 0.037
0.0GluXaa: 0.0 ± 0.0
Phe
4.038PheAla: 4.038 ± 0.058
0.238PheCys: 0.238 ± 0.015
2.183PheAsp: 2.183 ± 0.046
1.703PheGlu: 1.703 ± 0.041
1.188PhePhe: 1.188 ± 0.036
3.562PheGly: 3.562 ± 0.061
0.691PheHis: 0.691 ± 0.024
1.354PheIle: 1.354 ± 0.034
0.835PheLys: 0.835 ± 0.024
3.092PheLeu: 3.092 ± 0.068
0.613PheMet: 0.613 ± 0.024
0.918PheAsn: 0.918 ± 0.03
1.547PhePro: 1.547 ± 0.036
0.863PheGln: 0.863 ± 0.028
1.932PheArg: 1.932 ± 0.04
2.018PheSer: 2.018 ± 0.046
2.096PheThr: 2.096 ± 0.044
2.49PheVal: 2.49 ± 0.049
0.473PheTrp: 0.473 ± 0.024
0.755PheTyr: 0.755 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
10.454GlyAla: 10.454 ± 0.119
0.649GlyCys: 0.649 ± 0.028
4.212GlyAsp: 4.212 ± 0.071
4.407GlyGlu: 4.407 ± 0.067
3.386GlyPhe: 3.386 ± 0.057
8.012GlyGly: 8.012 ± 0.115
2.161GlyHis: 2.161 ± 0.054
5.019GlyIle: 5.019 ± 0.073
3.095GlyLys: 3.095 ± 0.06
9.536GlyLeu: 9.536 ± 0.093
2.13GlyMet: 2.13 ± 0.045
2.339GlyAsn: 2.339 ± 0.05
4.437GlyPro: 4.437 ± 0.069
3.179GlyGln: 3.179 ± 0.058
6.365GlyArg: 6.365 ± 0.077
6.052GlySer: 6.052 ± 0.081
6.337GlyThr: 6.337 ± 0.086
7.317GlyVal: 7.317 ± 0.081
1.635GlyTrp: 1.635 ± 0.04
2.407GlyTyr: 2.407 ± 0.048
0.0GlyXaa: 0.0 ± 0.0
His
2.339HisAla: 2.339 ± 0.046
0.149HisCys: 0.149 ± 0.011
1.149HisAsp: 1.149 ± 0.034
1.193HisGlu: 1.193 ± 0.034
0.719HisPhe: 0.719 ± 0.026
2.15HisGly: 2.15 ± 0.041
0.635HisHis: 0.635 ± 0.025
0.889HisIle: 0.889 ± 0.026
0.489HisLys: 0.489 ± 0.022
2.061HisLeu: 2.061 ± 0.048
0.363HisMet: 0.363 ± 0.017
0.513HisAsn: 0.513 ± 0.021
1.507HisPro: 1.507 ± 0.035
0.622HisGln: 0.622 ± 0.024
1.555HisArg: 1.555 ± 0.036
1.196HisSer: 1.196 ± 0.034
1.085HisThr: 1.085 ± 0.032
1.527HisVal: 1.527 ± 0.036
0.323HisTrp: 0.323 ± 0.018
0.466HisTyr: 0.466 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
5.925IleAla: 5.925 ± 0.075
0.369IleCys: 0.369 ± 0.017
2.74IleAsp: 2.74 ± 0.055
2.433IleGlu: 2.433 ± 0.045
1.405IlePhe: 1.405 ± 0.043
4.466IleGly: 4.466 ± 0.07
0.874IleHis: 0.874 ± 0.029
2.016IleIle: 2.016 ± 0.046
1.191IleLys: 1.191 ± 0.038
3.929IleLeu: 3.929 ± 0.065
0.821IleMet: 0.821 ± 0.026
1.298IleAsn: 1.298 ± 0.038
2.46IlePro: 2.46 ± 0.047
1.155IleGln: 1.155 ± 0.032
2.904IleArg: 2.904 ± 0.052
2.735IleSer: 2.735 ± 0.041
2.813IleThr: 2.813 ± 0.053
3.793IleVal: 3.793 ± 0.065
0.497IleTrp: 0.497 ± 0.022
0.876IleTyr: 0.876 ± 0.027
0.0IleXaa: 0.0 ± 0.0
Lys
3.665LysAla: 3.665 ± 0.062
0.104LysCys: 0.104 ± 0.01
1.756LysAsp: 1.756 ± 0.045
1.471LysGlu: 1.471 ± 0.04
0.811LysPhe: 0.811 ± 0.026
2.077LysGly: 2.077 ± 0.04
0.58LysHis: 0.58 ± 0.019
1.359LysIle: 1.359 ± 0.035
1.018LysLys: 1.018 ± 0.038
2.789LysLeu: 2.789 ± 0.051
0.63LysMet: 0.63 ± 0.023
0.78LysAsn: 0.78 ± 0.026
1.684LysPro: 1.684 ± 0.041
0.858LysGln: 0.858 ± 0.029
1.724LysArg: 1.724 ± 0.043
1.647LysSer: 1.647 ± 0.04
1.609LysThr: 1.609 ± 0.038
2.353LysVal: 2.353 ± 0.047
0.329LysTrp: 0.329 ± 0.019
0.664LysTyr: 0.664 ± 0.025
0.0LysXaa: 0.0 ± 0.0
Leu
14.844LeuAla: 14.844 ± 0.155
0.629LeuCys: 0.629 ± 0.023
6.315LeuAsp: 6.315 ± 0.081
5.369LeuGlu: 5.369 ± 0.076
2.95LeuPhe: 2.95 ± 0.062
9.447LeuGly: 9.447 ± 0.109
2.056LeuHis: 2.056 ± 0.04
4.408LeuIle: 4.408 ± 0.075
2.824LeuLys: 2.824 ± 0.053
10.712LeuLeu: 10.712 ± 0.154
2.0LeuMet: 2.0 ± 0.045
2.506LeuAsn: 2.506 ± 0.047
5.8LeuPro: 5.8 ± 0.076
2.946LeuGln: 2.946 ± 0.046
6.996LeuArg: 6.996 ± 0.089
6.01LeuSer: 6.01 ± 0.075
5.968LeuThr: 5.968 ± 0.073
8.293LeuVal: 8.293 ± 0.093
1.251LeuTrp: 1.251 ± 0.038
1.735LeuTyr: 1.735 ± 0.037
0.0LeuXaa: 0.0 ± 0.0
Met
2.641MetAla: 2.641 ± 0.05
0.121MetCys: 0.121 ± 0.009
1.058MetAsp: 1.058 ± 0.031
0.925MetGlu: 0.925 ± 0.026
0.617MetPhe: 0.617 ± 0.023
1.578MetGly: 1.578 ± 0.041
0.36MetHis: 0.36 ± 0.02
0.973MetIle: 0.973 ± 0.032
0.632MetLys: 0.632 ± 0.023
1.961MetLeu: 1.961 ± 0.045
0.406MetMet: 0.406 ± 0.018
0.562MetAsn: 0.562 ± 0.023
1.132MetPro: 1.132 ± 0.026
0.576MetGln: 0.576 ± 0.021
1.167MetArg: 1.167 ± 0.032
1.507MetSer: 1.507 ± 0.039
1.649MetThr: 1.649 ± 0.036
1.552MetVal: 1.552 ± 0.041
0.206MetTrp: 0.206 ± 0.014
0.307MetTyr: 0.307 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
2.866AsnAla: 2.866 ± 0.061
0.162AsnCys: 0.162 ± 0.012
1.24AsnAsp: 1.24 ± 0.034
1.182AsnGlu: 1.182 ± 0.03
0.841AsnPhe: 0.841 ± 0.026
2.37AsnGly: 2.37 ± 0.046
0.524AsnHis: 0.524 ± 0.019
1.087AsnIle: 1.087 ± 0.033
0.698AsnLys: 0.698 ± 0.025
2.303AsnLeu: 2.303 ± 0.048
0.481AsnMet: 0.481 ± 0.022
0.681AsnAsn: 0.681 ± 0.028
1.866AsnPro: 1.866 ± 0.04
0.768AsnGln: 0.768 ± 0.03
1.536AsnArg: 1.536 ± 0.039
1.331AsnSer: 1.331 ± 0.042
1.408AsnThr: 1.408 ± 0.035
1.906AsnVal: 1.906 ± 0.042
0.378AsnTrp: 0.378 ± 0.02
0.596AsnTyr: 0.596 ± 0.023
0.0AsnXaa: 0.0 ± 0.0
Pro
8.405ProAla: 8.405 ± 0.119
0.242ProCys: 0.242 ± 0.015
3.643ProAsp: 3.643 ± 0.061
4.052ProGlu: 4.052 ± 0.065
1.7ProPhe: 1.7 ± 0.04
6.066ProGly: 6.066 ± 0.074
1.068ProHis: 1.068 ± 0.034
1.703ProIle: 1.703 ± 0.041
1.345ProLys: 1.345 ± 0.036
5.062ProLeu: 5.062 ± 0.064
1.022ProMet: 1.022 ± 0.026
1.137ProAsn: 1.137 ± 0.029
2.22ProPro: 2.22 ± 0.054
1.583ProGln: 1.583 ± 0.04
2.98ProArg: 2.98 ± 0.059
3.221ProSer: 3.221 ± 0.053
2.637ProThr: 2.637 ± 0.054
4.852ProVal: 4.852 ± 0.068
0.798ProTrp: 0.798 ± 0.026
1.112ProTyr: 1.112 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
4.026GlnAla: 4.026 ± 0.065
0.159GlnCys: 0.159 ± 0.011
1.61GlnAsp: 1.61 ± 0.044
1.584GlnGlu: 1.584 ± 0.045
0.899GlnPhe: 0.899 ± 0.036
2.374GlnGly: 2.374 ± 0.047
0.727GlnHis: 0.727 ± 0.026
1.406GlnIle: 1.406 ± 0.034
0.821GlnLys: 0.821 ± 0.029
3.896GlnLeu: 3.896 ± 0.07
0.594GlnMet: 0.594 ± 0.027
0.742GlnAsn: 0.742 ± 0.026
1.724GlnPro: 1.724 ± 0.043
1.29GlnGln: 1.29 ± 0.034
2.287GlnArg: 2.287 ± 0.047
1.602GlnSer: 1.602 ± 0.039
1.499GlnThr: 1.499 ± 0.038
2.229GlnVal: 2.229 ± 0.048
0.477GlnTrp: 0.477 ± 0.018
0.686GlnTyr: 0.686 ± 0.023
0.0GlnXaa: 0.0 ± 0.0
Arg
7.184ArgAla: 7.184 ± 0.083
0.417ArgCys: 0.417 ± 0.02
3.444ArgAsp: 3.444 ± 0.059
3.752ArgGlu: 3.752 ± 0.058
2.259ArgPhe: 2.259 ± 0.041
4.918ArgGly: 4.918 ± 0.069
1.631ArgHis: 1.631 ± 0.037
3.491ArgIle: 3.491 ± 0.054
2.065ArgLys: 2.065 ± 0.052
7.166ArgLeu: 7.166 ± 0.088
1.521ArgMet: 1.521 ± 0.036
1.802ArgAsn: 1.802 ± 0.039
3.618ArgPro: 3.618 ± 0.065
2.323ArgGln: 2.323 ± 0.044
5.526ArgArg: 5.526 ± 0.083
3.869ArgSer: 3.869 ± 0.068
3.955ArgThr: 3.955 ± 0.065
4.633ArgVal: 4.633 ± 0.069
1.103ArgTrp: 1.103 ± 0.029
1.565ArgTyr: 1.565 ± 0.037
0.0ArgXaa: 0.0 ± 0.0
Ser
7.283SerAla: 7.283 ± 0.087
0.375SerCys: 0.375 ± 0.019
2.73SerAsp: 2.73 ± 0.047
2.738SerGlu: 2.738 ± 0.056
2.058SerPhe: 2.058 ± 0.044
6.266SerGly: 6.266 ± 0.07
1.145SerHis: 1.145 ± 0.031
2.511SerIle: 2.511 ± 0.047
1.53SerLys: 1.53 ± 0.037
5.818SerLeu: 5.818 ± 0.078
1.296SerMet: 1.296 ± 0.033
1.381SerAsn: 1.381 ± 0.037
3.28SerPro: 3.28 ± 0.058
1.687SerGln: 1.687 ± 0.039
3.959SerArg: 3.959 ± 0.059
3.543SerSer: 3.543 ± 0.061
3.383SerThr: 3.383 ± 0.062
4.649SerVal: 4.649 ± 0.077
0.943SerTrp: 0.943 ± 0.031
1.465SerTyr: 1.465 ± 0.033
0.0SerXaa: 0.0 ± 0.0
Thr
8.122ThrAla: 8.122 ± 0.095
0.281ThrCys: 0.281 ± 0.016
3.087ThrAsp: 3.087 ± 0.053
2.96ThrGlu: 2.96 ± 0.049
1.87ThrPhe: 1.87 ± 0.039
6.055ThrGly: 6.055 ± 0.076
1.109ThrHis: 1.109 ± 0.029
2.49ThrIle: 2.49 ± 0.051
1.378ThrLys: 1.378 ± 0.034
5.573ThrLeu: 5.573 ± 0.066
1.053ThrMet: 1.053 ± 0.03
1.219ThrAsn: 1.219 ± 0.034
3.716ThrPro: 3.716 ± 0.058
1.422ThrGln: 1.422 ± 0.038
3.308ThrArg: 3.308 ± 0.053
3.23ThrSer: 3.23 ± 0.057
3.362ThrThr: 3.362 ± 0.056
5.614ThrVal: 5.614 ± 0.072
0.665ThrTrp: 0.665 ± 0.022
1.075ThrTyr: 1.075 ± 0.03
0.0ThrXaa: 0.0 ± 0.0
Val
10.227ValAla: 10.227 ± 0.108
0.571ValCys: 0.571 ± 0.021
4.766ValAsp: 4.766 ± 0.077
4.568ValGlu: 4.568 ± 0.062
2.786ValPhe: 2.786 ± 0.055
6.301ValGly: 6.301 ± 0.071
1.631ValHis: 1.631 ± 0.041
4.08ValIle: 4.08 ± 0.058
2.124ValLys: 2.124 ± 0.052
9.19ValLeu: 9.19 ± 0.107
1.619ValMet: 1.619 ± 0.037
2.018ValAsn: 2.018 ± 0.042
4.805ValPro: 4.805 ± 0.064
2.409ValGln: 2.409 ± 0.045
5.349ValArg: 5.349 ± 0.079
4.905ValSer: 4.905 ± 0.065
4.998ValThr: 4.998 ± 0.073
7.598ValVal: 7.598 ± 0.089
0.986ValTrp: 0.986 ± 0.032
1.471ValTyr: 1.471 ± 0.038
0.0ValXaa: 0.0 ± 0.0
Trp
1.487TrpAla: 1.487 ± 0.038
0.105TrpCys: 0.105 ± 0.009
0.733TrpAsp: 0.733 ± 0.024
0.645TrpGlu: 0.645 ± 0.022
0.538TrpPhe: 0.538 ± 0.025
1.011TrpGly: 1.011 ± 0.025
0.339TrpHis: 0.339 ± 0.016
0.688TrpIle: 0.688 ± 0.025
0.415TrpLys: 0.415 ± 0.019
1.796TrpLeu: 1.796 ± 0.044
0.307TrpMet: 0.307 ± 0.015
0.441TrpAsn: 0.441 ± 0.018
0.664TrpPro: 0.664 ± 0.024
0.572TrpGln: 0.572 ± 0.023
0.993TrpArg: 0.993 ± 0.03
0.832TrpSer: 0.832 ± 0.029
0.891TrpThr: 0.891 ± 0.029
1.001TrpVal: 1.001 ± 0.031
0.281TrpTrp: 0.281 ± 0.017
0.285TrpTyr: 0.285 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.363TyrAla: 2.363 ± 0.044
0.164TyrCys: 0.164 ± 0.012
1.177TyrAsp: 1.177 ± 0.035
1.125TyrGlu: 1.125 ± 0.035
0.853TyrPhe: 0.853 ± 0.026
2.089TyrGly: 2.089 ± 0.048
0.376TyrHis: 0.376 ± 0.018
0.764TyrIle: 0.764 ± 0.023
0.541TyrLys: 0.541 ± 0.022
2.275TyrLeu: 2.275 ± 0.046
0.344TyrMet: 0.344 ± 0.016
0.54TyrAsn: 0.54 ± 0.023
1.136TyrPro: 1.136 ± 0.029
0.654TyrGln: 0.654 ± 0.023
1.58TyrArg: 1.58 ± 0.037
1.268TyrSer: 1.268 ± 0.029
1.127TyrThr: 1.127 ± 0.037
1.578TyrVal: 1.578 ± 0.035
0.332TyrTrp: 0.332 ± 0.016
0.572TyrTyr: 0.572 ± 0.024
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3600 proteins (1191758 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski