Amino acid dipepetide frequency for Azotobacter chroococcum NCIMB 8003

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.82AlaAla: 14.82 ± 0.157	1.467AlaCys: 1.467 ± 0.036	5.994AlaAsp: 5.994 ± 0.066	8.491AlaGlu: 8.491 ± 0.1	3.898AlaPhe: 3.898 ± 0.055	10.473AlaGly: 10.473 ± 0.102	2.419AlaHis: 2.419 ± 0.04	4.919AlaIle: 4.919 ± 0.061	3.134AlaLys: 3.134 ± 0.054	15.09AlaLeu: 15.09 ± 0.134	2.747AlaMet: 2.747 ± 0.045	2.497AlaAsn: 2.497 ± 0.043	5.337AlaPro: 5.337 ± 0.081	4.704AlaGln: 4.704 ± 0.074	9.261AlaArg: 9.261 ± 0.089	6.062AlaSer: 6.062 ± 0.067	4.573AlaThr: 4.573 ± 0.063	7.505AlaVal: 7.505 ± 0.083	1.799AlaTrp: 1.799 ± 0.038	2.388AlaTyr: 2.388 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	1.26CysAla: 1.26 ± 0.035	0.178CysCys: 0.178 ± 0.01	0.562CysAsp: 0.562 ± 0.021	0.608CysGlu: 0.608 ± 0.02	0.364CysPhe: 0.364 ± 0.017	1.058CysGly: 1.058 ± 0.035	0.307CysHis: 0.307 ± 0.015	0.41CysIle: 0.41 ± 0.018	0.267CysLys: 0.267 ± 0.013	1.213CysLeu: 1.213 ± 0.034	0.192CysMet: 0.192 ± 0.013	0.245CysAsn: 0.245 ± 0.011	0.613CysPro: 0.613 ± 0.022	0.395CysGln: 0.395 ± 0.018	0.867CysArg: 0.867 ± 0.027	0.617CysSer: 0.617 ± 0.022	0.488CysThr: 0.488 ± 0.018	0.661CysVal: 0.661 ± 0.024	0.156CysTrp: 0.156 ± 0.01	0.252CysTyr: 0.252 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	5.595AspAla: 5.595 ± 0.055	0.629AspCys: 0.629 ± 0.023	2.752AspAsp: 2.752 ± 0.058	3.646AspGlu: 3.646 ± 0.052	2.181AspPhe: 2.181 ± 0.043	4.749AspGly: 4.749 ± 0.076	1.111AspHis: 1.111 ± 0.028	2.422AspIle: 2.422 ± 0.041	1.656AspLys: 1.656 ± 0.036	6.01AspLeu: 6.01 ± 0.065	1.077AspMet: 1.077 ± 0.026	1.407AspAsn: 1.407 ± 0.043	3.001AspPro: 3.001 ± 0.049	1.705AspGln: 1.705 ± 0.038	3.489AspArg: 3.489 ± 0.046	2.84AspSer: 2.84 ± 0.052	2.271AspThr: 2.271 ± 0.056	3.016AspVal: 3.016 ± 0.048	1.054AspTrp: 1.054 ± 0.031	1.706AspTyr: 1.706 ± 0.034	0.0AspXaa: 0.0 ± 0.0
Glu	7.952GluAla: 7.952 ± 0.097	0.516GluCys: 0.516 ± 0.022	2.686GluAsp: 2.686 ± 0.042	3.774GluGlu: 3.774 ± 0.08	1.849GluPhe: 1.849 ± 0.038	4.485GluGly: 4.485 ± 0.062	1.698GluHis: 1.698 ± 0.033	2.917GluIle: 2.917 ± 0.047	2.077GluLys: 2.077 ± 0.048	7.819GluLeu: 7.819 ± 0.091	1.314GluMet: 1.314 ± 0.032	1.497GluAsn: 1.497 ± 0.038	3.01GluPro: 3.01 ± 0.057	3.626GluGln: 3.626 ± 0.066	6.419GluArg: 6.419 ± 0.082	2.759GluSer: 2.759 ± 0.039	2.724GluThr: 2.724 ± 0.043	4.391GluVal: 4.391 ± 0.058	0.788GluTrp: 0.788 ± 0.025	1.239GluTyr: 1.239 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	4.314PheAla: 4.314 ± 0.051	0.447PheCys: 0.447 ± 0.018	2.385PheAsp: 2.385 ± 0.044	2.194PheGlu: 2.194 ± 0.038	1.401PhePhe: 1.401 ± 0.037	3.266PheGly: 3.266 ± 0.051	0.793PheHis: 0.793 ± 0.024	1.528PheIle: 1.528 ± 0.038	1.048PheLys: 1.048 ± 0.033	3.339PheLeu: 3.339 ± 0.052	0.698PheMet: 0.698 ± 0.024	1.075PheAsn: 1.075 ± 0.031	1.486PhePro: 1.486 ± 0.03	1.125PheGln: 1.125 ± 0.026	2.189PheArg: 2.189 ± 0.039	2.167PheSer: 2.167 ± 0.044	1.607PheThr: 1.607 ± 0.035	2.573PheVal: 2.573 ± 0.044	0.555PheTrp: 0.555 ± 0.021	0.979PheTyr: 0.979 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	7.974GlyAla: 7.974 ± 0.091	1.056GlyCys: 1.056 ± 0.027	4.061GlyAsp: 4.061 ± 0.06	5.664GlyGlu: 5.664 ± 0.07	3.298GlyPhe: 3.298 ± 0.05	6.531GlyGly: 6.531 ± 0.087	1.982GlyHis: 1.982 ± 0.035	4.002GlyIle: 4.002 ± 0.064	3.146GlyLys: 3.146 ± 0.053	9.847GlyLeu: 9.847 ± 0.104	2.08GlyMet: 2.08 ± 0.042	2.301GlyAsn: 2.301 ± 0.091	2.915GlyPro: 2.915 ± 0.047	3.548GlyGln: 3.548 ± 0.048	6.282GlyArg: 6.282 ± 0.072	4.65GlySer: 4.65 ± 0.068	3.66GlyThr: 3.66 ± 0.055	5.813GlyVal: 5.813 ± 0.067	1.487GlyTrp: 1.487 ± 0.033	2.397GlyTyr: 2.397 ± 0.041	0.0GlyXaa: 0.0 ± 0.0
His	2.508HisAla: 2.508 ± 0.04	0.364HisCys: 0.364 ± 0.015	1.24HisAsp: 1.24 ± 0.028	1.287HisGlu: 1.287 ± 0.031	1.004HisPhe: 1.004 ± 0.027	2.182HisGly: 2.182 ± 0.043	0.672HisHis: 0.672 ± 0.025	0.926HisIle: 0.926 ± 0.026	0.624HisLys: 0.624 ± 0.021	2.748HisLeu: 2.748 ± 0.041	0.469HisMet: 0.469 ± 0.019	0.591HisAsn: 0.591 ± 0.021	1.66HisPro: 1.66 ± 0.041	0.839HisGln: 0.839 ± 0.026	1.649HisArg: 1.649 ± 0.039	1.216HisSer: 1.216 ± 0.032	0.925HisThr: 0.925 ± 0.027	1.358HisVal: 1.358 ± 0.028	0.48HisTrp: 0.48 ± 0.018	0.81HisTyr: 0.81 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.573IleAla: 5.573 ± 0.073	0.441IleCys: 0.441 ± 0.017	3.043IleAsp: 3.043 ± 0.055	3.248IleGlu: 3.248 ± 0.053	1.267IlePhe: 1.267 ± 0.033	4.146IleGly: 4.146 ± 0.06	1.044IleHis: 1.044 ± 0.024	1.448IleIle: 1.448 ± 0.041	1.412IleLys: 1.412 ± 0.031	4.07IleLeu: 4.07 ± 0.056	0.698IleMet: 0.698 ± 0.023	1.256IleAsn: 1.256 ± 0.032	2.175IlePro: 2.175 ± 0.041	1.421IleGln: 1.421 ± 0.031	3.151IleArg: 3.151 ± 0.049	2.184IleSer: 2.184 ± 0.048	1.946IleThr: 1.946 ± 0.036	3.032IleVal: 3.032 ± 0.049	0.426IleTrp: 0.426 ± 0.016	0.976IleTyr: 0.976 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	3.764LysAla: 3.764 ± 0.065	0.181LysCys: 0.181 ± 0.011	1.54LysAsp: 1.54 ± 0.035	1.619LysGlu: 1.619 ± 0.04	0.745LysPhe: 0.745 ± 0.026	2.407LysGly: 2.407 ± 0.047	0.663LysHis: 0.663 ± 0.022	1.347LysIle: 1.347 ± 0.032	1.249LysLys: 1.249 ± 0.04	3.21LysLeu: 3.21 ± 0.056	0.572LysMet: 0.572 ± 0.022	0.816LysAsn: 0.816 ± 0.027	1.836LysPro: 1.836 ± 0.041	1.247LysGln: 1.247 ± 0.035	2.349LysArg: 2.349 ± 0.04	1.71LysSer: 1.71 ± 0.041	1.626LysThr: 1.626 ± 0.043	2.33LysVal: 2.33 ± 0.042	0.323LysTrp: 0.323 ± 0.016	0.602LysTyr: 0.602 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	16.483LeuAla: 16.483 ± 0.132	1.295LeuCys: 1.295 ± 0.035	7.129LeuAsp: 7.129 ± 0.085	7.759LeuGlu: 7.759 ± 0.093	4.232LeuPhe: 4.232 ± 0.059	9.993LeuGly: 9.993 ± 0.096	2.655LeuHis: 2.655 ± 0.043	5.101LeuIle: 5.101 ± 0.059	3.913LeuLys: 3.913 ± 0.057	15.001LeuLeu: 15.001 ± 0.179	2.153LeuMet: 2.153 ± 0.039	3.087LeuAsn: 3.087 ± 0.049	7.053LeuPro: 7.053 ± 0.079	4.688LeuGln: 4.688 ± 0.064	8.609LeuArg: 8.609 ± 0.085	6.646LeuSer: 6.646 ± 0.072	5.118LeuThr: 5.118 ± 0.065	7.903LeuVal: 7.903 ± 0.082	1.496LeuTrp: 1.496 ± 0.035	2.67LeuTyr: 2.67 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	2.447MetAla: 2.447 ± 0.041	0.14MetCys: 0.14 ± 0.009	0.955MetAsp: 0.955 ± 0.025	0.974MetGlu: 0.974 ± 0.032	0.568MetPhe: 0.568 ± 0.021	1.488MetGly: 1.488 ± 0.034	0.493MetHis: 0.493 ± 0.019	0.898MetIle: 0.898 ± 0.027	0.801MetLys: 0.801 ± 0.025	2.567MetLeu: 2.567 ± 0.039	0.412MetMet: 0.412 ± 0.017	0.692MetAsn: 0.692 ± 0.022	1.302MetPro: 1.302 ± 0.028	0.951MetGln: 0.951 ± 0.027	1.448MetArg: 1.448 ± 0.031	1.428MetSer: 1.428 ± 0.032	1.244MetThr: 1.244 ± 0.032	1.337MetVal: 1.337 ± 0.026	0.147MetTrp: 0.147 ± 0.011	0.298MetTyr: 0.298 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.745AsnAla: 2.745 ± 0.051	0.292AsnCys: 0.292 ± 0.014	1.278AsnAsp: 1.278 ± 0.05	1.237AsnGlu: 1.237 ± 0.032	0.949AsnPhe: 0.949 ± 0.027	2.183AsnGly: 2.183 ± 0.051	0.593AsnHis: 0.593 ± 0.022	1.11AsnIle: 1.11 ± 0.033	0.717AsnLys: 0.717 ± 0.025	3.089AsnLeu: 3.089 ± 0.054	0.488AsnMet: 0.488 ± 0.018	0.699AsnAsn: 0.699 ± 0.032	1.886AsnPro: 1.886 ± 0.042	0.933AsnGln: 0.933 ± 0.027	1.875AsnArg: 1.875 ± 0.042	1.264AsnSer: 1.264 ± 0.035	1.18AsnThr: 1.18 ± 0.032	1.641AsnVal: 1.641 ± 0.041	0.388AsnTrp: 0.388 ± 0.018	0.69AsnTyr: 0.69 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	6.862ProAla: 6.862 ± 0.099	0.431ProCys: 0.431 ± 0.019	2.86ProAsp: 2.86 ± 0.045	4.013ProGlu: 4.013 ± 0.058	1.806ProPhe: 1.806 ± 0.033	4.598ProGly: 4.598 ± 0.062	1.122ProHis: 1.122 ± 0.032	1.872ProIle: 1.872 ± 0.034	1.416ProLys: 1.416 ± 0.038	6.399ProLeu: 6.399 ± 0.077	1.084ProMet: 1.084 ± 0.027	1.207ProAsn: 1.207 ± 0.033	2.567ProPro: 2.567 ± 0.054	2.302ProGln: 2.302 ± 0.037	3.228ProArg: 3.228 ± 0.043	2.527ProSer: 2.527 ± 0.041	2.038ProThr: 2.038 ± 0.043	3.854ProVal: 3.854 ± 0.061	0.817ProTrp: 0.817 ± 0.023	1.213ProTyr: 1.213 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	5.927GlnAla: 5.927 ± 0.08	0.341GlnCys: 0.341 ± 0.015	1.787GlnAsp: 1.787 ± 0.035	2.067GlnGlu: 2.067 ± 0.044	1.173GlnPhe: 1.173 ± 0.027	3.273GlnGly: 3.273 ± 0.048	0.975GlnHis: 0.975 ± 0.027	1.748GlnIle: 1.748 ± 0.035	1.085GlnLys: 1.085 ± 0.032	5.11GlnLeu: 5.11 ± 0.076	0.833GlnMet: 0.833 ± 0.023	0.891GlnAsn: 0.891 ± 0.026	2.466GlnPro: 2.466 ± 0.05	2.179GlnGln: 2.179 ± 0.052	3.603GlnArg: 3.603 ± 0.054	1.914GlnSer: 1.914 ± 0.035	1.681GlnThr: 1.681 ± 0.035	3.179GlnVal: 3.179 ± 0.044	0.598GlnTrp: 0.598 ± 0.021	0.908GlnTyr: 0.908 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	7.375ArgAla: 7.375 ± 0.083	0.722ArgCys: 0.722 ± 0.023	3.772ArgAsp: 3.772 ± 0.054	5.234ArgGlu: 5.234 ± 0.07	3.092ArgPhe: 3.092 ± 0.051	4.749ArgGly: 4.749 ± 0.063	2.256ArgHis: 2.256 ± 0.046	3.761ArgIle: 3.761 ± 0.048	2.094ArgLys: 2.094 ± 0.044	10.838ArgLeu: 10.838 ± 0.105	1.626ArgMet: 1.626 ± 0.033	1.925ArgAsn: 1.925 ± 0.042	3.714ArgPro: 3.714 ± 0.049	4.221ArgGln: 4.221 ± 0.065	6.206ArgArg: 6.206 ± 0.087	3.62ArgSer: 3.62 ± 0.05	2.924ArgThr: 2.924 ± 0.047	4.852ArgVal: 4.852 ± 0.056	1.23ArgTrp: 1.23 ± 0.027	2.221ArgTyr: 2.221 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	5.851SerAla: 5.851 ± 0.061	0.552SerCys: 0.552 ± 0.02	2.51SerAsp: 2.51 ± 0.051	2.905SerGlu: 2.905 ± 0.042	1.956SerPhe: 1.956 ± 0.037	5.125SerGly: 5.125 ± 0.076	1.335SerHis: 1.335 ± 0.031	2.274SerIle: 2.274 ± 0.044	1.423SerLys: 1.423 ± 0.038	6.824SerLeu: 6.824 ± 0.079	1.196SerMet: 1.196 ± 0.029	1.278SerAsn: 1.278 ± 0.033	2.8SerPro: 2.8 ± 0.049	1.92SerGln: 1.92 ± 0.043	3.917SerArg: 3.917 ± 0.054	2.932SerSer: 2.932 ± 0.059	2.444SerThr: 2.444 ± 0.043	3.405SerVal: 3.405 ± 0.053	0.801SerTrp: 0.801 ± 0.026	1.268SerTyr: 1.268 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	4.682ThrAla: 4.682 ± 0.062	0.467ThrCys: 0.467 ± 0.021	2.08ThrAsp: 2.08 ± 0.045	2.242ThrGlu: 2.242 ± 0.041	1.537ThrPhe: 1.537 ± 0.035	3.875ThrGly: 3.875 ± 0.065	1.026ThrHis: 1.026 ± 0.028	1.682ThrIle: 1.682 ± 0.035	0.904ThrLys: 0.904 ± 0.026	6.299ThrLeu: 6.299 ± 0.082	0.722ThrMet: 0.722 ± 0.025	0.91ThrAsn: 0.91 ± 0.026	3.008ThrPro: 3.008 ± 0.048	1.49ThrGln: 1.49 ± 0.036	3.197ThrArg: 3.197 ± 0.052	2.277ThrSer: 2.277 ± 0.05	2.004ThrThr: 2.004 ± 0.048	3.029ThrVal: 3.029 ± 0.053	0.625ThrTrp: 0.625 ± 0.02	1.081ThrTyr: 1.081 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	7.772ValAla: 7.772 ± 0.083	0.741ValCys: 0.741 ± 0.023	3.795ValAsp: 3.795 ± 0.054	4.708ValGlu: 4.708 ± 0.058	2.404ValPhe: 2.404 ± 0.048	5.141ValGly: 5.141 ± 0.064	1.459ValHis: 1.459 ± 0.028	3.134ValIle: 3.134 ± 0.055	2.073ValLys: 2.073 ± 0.045	8.056ValLeu: 8.056 ± 0.081	1.469ValMet: 1.469 ± 0.034	1.777ValAsn: 1.777 ± 0.032	3.351ValPro: 3.351 ± 0.057	2.407ValGln: 2.407 ± 0.046	4.891ValArg: 4.891 ± 0.058	3.66ValSer: 3.66 ± 0.056	3.092ValThr: 3.092 ± 0.048	5.082ValVal: 5.082 ± 0.072	0.839ValTrp: 0.839 ± 0.024	1.52ValTyr: 1.52 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.16TrpAla: 1.16 ± 0.028	0.163TrpCys: 0.163 ± 0.01	0.607TrpAsp: 0.607 ± 0.021	0.649TrpGlu: 0.649 ± 0.021	0.538TrpPhe: 0.538 ± 0.019	0.906TrpGly: 0.906 ± 0.029	0.393TrpHis: 0.393 ± 0.017	0.6TrpIle: 0.6 ± 0.019	0.412TrpLys: 0.412 ± 0.017	2.382TrpLeu: 2.382 ± 0.048	0.349TrpMet: 0.349 ± 0.017	0.431TrpAsn: 0.431 ± 0.017	0.757TrpPro: 0.757 ± 0.025	0.959TrpGln: 0.959 ± 0.03	1.405TrpArg: 1.405 ± 0.039	0.902TrpSer: 0.902 ± 0.025	0.594TrpThr: 0.594 ± 0.02	0.865TrpVal: 0.865 ± 0.025	0.247TrpTrp: 0.247 ± 0.015	0.345TrpTyr: 0.345 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.514TyrAla: 2.514 ± 0.049	0.29TyrCys: 0.29 ± 0.014	1.278TyrAsp: 1.278 ± 0.033	1.219TyrGlu: 1.219 ± 0.035	0.909TyrPhe: 0.909 ± 0.026	2.066TyrGly: 2.066 ± 0.042	0.558TyrHis: 0.558 ± 0.017	0.858TyrIle: 0.858 ± 0.026	0.684TyrLys: 0.684 ± 0.021	3.075TyrLeu: 3.075 ± 0.046	0.409TyrMet: 0.409 ± 0.017	0.654TyrAsn: 0.654 ± 0.02	1.28TyrPro: 1.28 ± 0.035	1.08TyrGln: 1.08 ± 0.029	2.397TyrArg: 2.397 ± 0.045	1.344TyrSer: 1.344 ± 0.033	0.995TyrThr: 0.995 ± 0.028	1.545TyrVal: 1.545 ± 0.034	0.408TyrTrp: 0.408 ± 0.02	0.691TyrTyr: 0.691 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4353 proteins (1419050 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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