Changjiang tombus-like virus 5

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG85|A0A1L3KG85_9VIRU RNA-directed RNA polymerase OS=Changjiang tombus-like virus 5 OX=1922819 PE=4 SV=1
MM1 pKa = 6.4YY2 pKa = 10.08QGRR5 pKa = 11.84RR6 pKa = 11.84KK7 pKa = 9.85EE8 pKa = 4.03IYY10 pKa = 9.67QRR12 pKa = 11.84AADD15 pKa = 3.96SLASRR20 pKa = 11.84PLVKK24 pKa = 10.18SDD26 pKa = 3.45AFMSTFLKK34 pKa = 10.71CEE36 pKa = 4.4KK37 pKa = 9.78IDD39 pKa = 4.01FSSKK43 pKa = 9.73PDD45 pKa = 3.62PAPRR49 pKa = 11.84VIQPRR54 pKa = 11.84TPRR57 pKa = 11.84YY58 pKa = 7.96NVEE61 pKa = 3.55VGRR64 pKa = 11.84YY65 pKa = 7.84LKK67 pKa = 10.35PLEE70 pKa = 3.89KK71 pKa = 10.0RR72 pKa = 11.84ICRR75 pKa = 11.84GIAEE79 pKa = 4.12IWGGDD84 pKa = 3.66TILKK88 pKa = 9.73GKK90 pKa = 9.68NAEE93 pKa = 3.95QSAVSLRR100 pKa = 11.84QMWDD104 pKa = 2.98QFDD107 pKa = 3.75EE108 pKa = 4.58PVAVGIDD115 pKa = 3.21ATRR118 pKa = 11.84FDD120 pKa = 3.33QHH122 pKa = 7.02VSYY125 pKa = 10.62EE126 pKa = 4.05ALEE129 pKa = 4.47WEE131 pKa = 4.3HH132 pKa = 7.06SVYY135 pKa = 10.1MLCFPPHH142 pKa = 5.89TRR144 pKa = 11.84PKK146 pKa = 10.63LEE148 pKa = 4.77RR149 pKa = 11.84LLKK152 pKa = 8.72MQLVNRR158 pKa = 11.84GYY160 pKa = 11.34ARR162 pKa = 11.84LEE164 pKa = 4.05DD165 pKa = 4.14LEE167 pKa = 4.94LMYY170 pKa = 10.41EE171 pKa = 4.06VRR173 pKa = 11.84GRR175 pKa = 11.84RR176 pKa = 11.84MSGDD180 pKa = 3.3MNTGMGNCLLMCAMIHH196 pKa = 6.94WITAEE201 pKa = 3.7MGVRR205 pKa = 11.84CRR207 pKa = 11.84LANNGDD213 pKa = 3.75DD214 pKa = 4.14CVLILEE220 pKa = 4.64KK221 pKa = 10.34RR222 pKa = 11.84DD223 pKa = 3.47LGKK226 pKa = 10.58LGRR229 pKa = 11.84LGPLALDD236 pKa = 3.85FGFVLEE242 pKa = 5.02IEE244 pKa = 4.53PEE246 pKa = 3.85VDD248 pKa = 3.52VFEE251 pKa = 5.33QICFCQNHH259 pKa = 5.93PVWGGHH265 pKa = 4.52SWVMCRR271 pKa = 11.84DD272 pKa = 3.24PRR274 pKa = 11.84KK275 pKa = 10.24CVDD278 pKa = 3.34KK279 pKa = 11.36DD280 pKa = 3.57LVTVLDD286 pKa = 4.21LGNIKK291 pKa = 9.81SARR294 pKa = 11.84KK295 pKa = 6.53WCHH298 pKa = 6.13AIGTGGLAMAGGLPVLGSFYY318 pKa = 11.49GMLLRR323 pKa = 11.84HH324 pKa = 5.73ATTGKK329 pKa = 10.23VDD331 pKa = 3.36AHH333 pKa = 6.38PWLEE337 pKa = 3.89NGFAMMAKK345 pKa = 10.02GMARR349 pKa = 11.84HH350 pKa = 6.09SDD352 pKa = 3.34VVTPEE357 pKa = 3.67SRR359 pKa = 11.84ASFFKK364 pKa = 11.03AFDD367 pKa = 3.44ITPDD371 pKa = 3.32QQEE374 pKa = 4.67SIEE377 pKa = 4.17SSYY380 pKa = 11.27ATMLLNLSAGVSEE393 pKa = 5.57SPLACYY399 pKa = 10.08GKK401 pKa = 9.55IWQQ404 pKa = 4.11

Molecular weight: 45.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFT1|A0A1L3KFT1_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 5 OX=1922819 PE=4 SV=1
MM1 pKa = 7.82AKK3 pKa = 9.19TPKK6 pKa = 10.45NKK8 pKa = 10.03GKK10 pKa = 9.04SQSVRR15 pKa = 11.84VLVTGGRR22 pKa = 11.84KK23 pKa = 9.26KK24 pKa = 10.29IKK26 pKa = 10.24RR27 pKa = 11.84KK28 pKa = 8.29ATAGNRR34 pKa = 11.84MGMSTLPARR43 pKa = 11.84WMRR46 pKa = 11.84LLTDD50 pKa = 3.86PCLADD55 pKa = 3.58LTYY58 pKa = 10.29PCYY61 pKa = 10.63GGTDD65 pKa = 2.67AGYY68 pKa = 10.59LVRR71 pKa = 11.84TVDD74 pKa = 3.53TFSPTIGGSFTVGSQVIADD93 pKa = 5.36GIYY96 pKa = 8.96TWSPWNLSATTGTRR110 pKa = 11.84SSFWTSGDD118 pKa = 3.37PLTPSSAGFNGNFISTAAVRR138 pKa = 11.84SYY140 pKa = 11.18RR141 pKa = 11.84PVASCLKK148 pKa = 8.63WVPIGPIGGRR158 pKa = 11.84SGLIASGYY166 pKa = 9.05SPGVVATPSVGVGNYY181 pKa = 6.81VTLAQRR187 pKa = 11.84KK188 pKa = 6.04ATNGTEE194 pKa = 3.65MHH196 pKa = 7.14EE197 pKa = 4.54INWLPTSHH205 pKa = 7.46DD206 pKa = 3.63EE207 pKa = 4.28EE208 pKa = 4.72FTTVEE213 pKa = 4.19QANSTSVGTVFMALKK228 pKa = 10.58GVDD231 pKa = 3.7AVAKK235 pKa = 9.93SSTSATLNGYY245 pKa = 8.86FEE247 pKa = 4.57ITTVWEE253 pKa = 3.99WTPATTTGLCVDD265 pKa = 4.46PRR267 pKa = 11.84TPSPYY272 pKa = 9.36TSQTVLSMFGDD283 pKa = 3.66IKK285 pKa = 11.07NALFAGAHH293 pKa = 4.69STAVGAAGQFGRR305 pKa = 11.84KK306 pKa = 8.01AVGMIGGVASSAAMAAIDD324 pKa = 3.7YY325 pKa = 10.1AGRR328 pKa = 11.84SFMGVANNPPRR339 pKa = 11.84YY340 pKa = 9.21RR341 pKa = 11.84GNSVPLIGYY350 pKa = 8.49

Molecular weight: 36.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

990

236

404

330.0

36.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.495 ± 1.092

2.222 ± 0.534

4.949 ± 0.743

4.747 ± 1.126

3.434 ± 0.169

9.091 ± 1.086

1.616 ± 0.438

3.838 ± 0.43

4.747 ± 0.311

8.283 ± 1.178

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.242 ± 0.576

3.03 ± 0.405

4.949 ± 0.523

2.121 ± 0.581

6.768 ± 0.833

6.97 ± 1.12

6.768 ± 1.947

7.98 ± 0.941

1.919 ± 0.33

2.828 ± 0.267
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski