Oryzias javanicus (Javanese ricefish) (Aplocheilus javanicus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri;

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21400 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A437CXI4|A0A437CXI4_ORYJA Uncharacterized protein OS=Oryzias javanicus OX=123683 GN=OJAV_G00101990 PE=3 SV=1
MM1 pKa = 7.08QPSALRR7 pKa = 11.84ALCSALLLLTVVPSVCSQSDD27 pKa = 3.66CSQADD32 pKa = 3.52SCDD35 pKa = 3.65LCVGDD40 pKa = 5.83SMLNLTGCVWRR51 pKa = 11.84LCPNGNDD58 pKa = 3.04TGMCVTDD65 pKa = 4.75GGDD68 pKa = 4.16SNDD71 pKa = 3.38TGMNCSWTRR80 pKa = 11.84VSEE83 pKa = 4.16LCTVVEE89 pKa = 4.51NVATGGEE96 pKa = 4.68GDD98 pKa = 4.03TGDD101 pKa = 4.78ASDD104 pKa = 3.9TTSSPEE110 pKa = 3.53FSQAKK115 pKa = 9.54FDD117 pKa = 3.55MSSFIGGIILVLSLQAGGFFAMRR140 pKa = 11.84FLKK143 pKa = 10.72SKK145 pKa = 9.46EE146 pKa = 3.68QSNYY150 pKa = 10.84DD151 pKa = 4.46PIEE154 pKa = 3.99QPQQ157 pKa = 3.0

Molecular weight: 16.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A437CM98|A0A437CM98_ORYJA Uncharacterized protein OS=Oryzias javanicus OX=123683 GN=OJAV_G00163900 PE=4 SV=1
MM1 pKa = 7.32EE2 pKa = 4.9FSYY5 pKa = 10.99GRR7 pKa = 11.84SFLTPTTNQRR17 pKa = 11.84LLKK20 pKa = 8.34EE21 pKa = 3.92THH23 pKa = 6.09LRR25 pKa = 11.84RR26 pKa = 11.84SGPLKK31 pKa = 9.74EE32 pKa = 4.31SPRR35 pKa = 11.84ARR37 pKa = 11.84WLISRR42 pKa = 11.84GTLNLSRR49 pKa = 11.84PPPPHH54 pKa = 6.49EE55 pKa = 4.23LPRR58 pKa = 11.84AQEE61 pKa = 4.06RR62 pKa = 11.84RR63 pKa = 11.84HH64 pKa = 5.43GAALYY69 pKa = 10.31ARR71 pKa = 11.84SAPAPEE77 pKa = 5.82LEE79 pKa = 4.27LQSNASLNRR88 pKa = 11.84ARR90 pKa = 11.84AGGRR94 pKa = 11.84GAGGSAHH101 pKa = 6.16EE102 pKa = 4.21RR103 pKa = 11.84AEE105 pKa = 4.08RR106 pKa = 11.84SQVGCRR112 pKa = 11.84EE113 pKa = 3.99LRR115 pKa = 11.84STKK118 pKa = 10.35YY119 pKa = 10.43ISDD122 pKa = 3.73GRR124 pKa = 11.84CTSINPIKK132 pKa = 10.47EE133 pKa = 4.01LVCAGEE139 pKa = 4.34CLPAQMFEE147 pKa = 3.61NWIGGRR153 pKa = 11.84RR154 pKa = 11.84YY155 pKa = 7.81WARR158 pKa = 11.84RR159 pKa = 11.84SGNDD163 pKa = 2.96DD164 pKa = 3.17WRR166 pKa = 11.84CVNDD170 pKa = 3.23KK171 pKa = 10.15TRR173 pKa = 11.84TQRR176 pKa = 11.84IQLQCQDD183 pKa = 3.43GSSRR187 pKa = 11.84TYY189 pKa = 10.45KK190 pKa = 8.86ITVVTSCKK198 pKa = 9.38CKK200 pKa = 10.21RR201 pKa = 11.84YY202 pKa = 9.78SRR204 pKa = 11.84QHH206 pKa = 5.8NEE208 pKa = 3.62SGGKK212 pKa = 9.71FEE214 pKa = 5.14EE215 pKa = 4.82PAAVPPKK222 pKa = 10.17LLHH225 pKa = 5.33KK226 pKa = 9.98HH227 pKa = 5.33KK228 pKa = 10.48SKK230 pKa = 10.56SRR232 pKa = 11.84RR233 pKa = 11.84RR234 pKa = 11.84LGKK237 pKa = 10.04SRR239 pKa = 11.84TRR241 pKa = 11.84EE242 pKa = 3.7NWHH245 pKa = 6.12DD246 pKa = 3.71TEE248 pKa = 4.38PP249 pKa = 3.93

Molecular weight: 28.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21345

55

21400

11474022

51

13616

536.2

59.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.747 ± 0.015

2.254 ± 0.014

5.151 ± 0.012

6.963 ± 0.021

3.633 ± 0.01

6.376 ± 0.022

2.584 ± 0.009

4.15 ± 0.012

5.612 ± 0.021

9.563 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.315 ± 0.008

3.73 ± 0.01

5.859 ± 0.022

4.761 ± 0.018

5.842 ± 0.014

8.979 ± 0.022

5.487 ± 0.015

6.267 ± 0.014

1.172 ± 0.005

2.553 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski