Amino acid dipepetide frequency for Streptomyces phage Rowa

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.044AlaAla: 13.044 ± 1.133
1.093AlaCys: 1.093 ± 0.345
9.138AlaAsp: 9.138 ± 0.734
8.436AlaGlu: 8.436 ± 1.156
3.28AlaPhe: 3.28 ± 0.629
9.217AlaGly: 9.217 ± 1.023
1.25AlaHis: 1.25 ± 0.267
4.452AlaIle: 4.452 ± 0.634
5.624AlaLys: 5.624 ± 0.904
11.482AlaLeu: 11.482 ± 0.838
3.046AlaMet: 3.046 ± 0.46
3.437AlaAsn: 3.437 ± 0.572
4.608AlaPro: 4.608 ± 0.819
3.827AlaGln: 3.827 ± 0.55
7.42AlaArg: 7.42 ± 0.754
5.624AlaSer: 5.624 ± 0.605
7.186AlaThr: 7.186 ± 0.983
9.373AlaVal: 9.373 ± 0.877
2.109AlaTrp: 2.109 ± 0.33
3.202AlaTyr: 3.202 ± 0.452
0.0AlaXaa: 0.0 ± 0.0
Cys
1.093CysAla: 1.093 ± 0.404
0.0CysCys: 0.0 ± 0.0
0.703CysAsp: 0.703 ± 0.225
0.703CysGlu: 0.703 ± 0.21
0.156CysPhe: 0.156 ± 0.097
0.937CysGly: 0.937 ± 0.271
0.156CysHis: 0.156 ± 0.118
0.391CysIle: 0.391 ± 0.159
0.547CysLys: 0.547 ± 0.269
0.781CysLeu: 0.781 ± 0.279
0.156CysMet: 0.156 ± 0.106
0.234CysAsn: 0.234 ± 0.145
0.859CysPro: 0.859 ± 0.34
0.391CysGln: 0.391 ± 0.159
0.859CysArg: 0.859 ± 0.272
0.703CysSer: 0.703 ± 0.195
0.781CysThr: 0.781 ± 0.297
0.703CysVal: 0.703 ± 0.233
0.312CysTrp: 0.312 ± 0.199
0.391CysTyr: 0.391 ± 0.176
0.0CysXaa: 0.0 ± 0.0
Asp
8.904AspAla: 8.904 ± 0.852
0.937AspCys: 0.937 ± 0.234
3.437AspAsp: 3.437 ± 0.504
3.905AspGlu: 3.905 ± 0.628
1.406AspPhe: 1.406 ± 0.29
6.014AspGly: 6.014 ± 0.567
1.172AspHis: 1.172 ± 0.284
2.499AspIle: 2.499 ± 0.425
2.656AspLys: 2.656 ± 0.455
5.467AspLeu: 5.467 ± 0.704
1.172AspMet: 1.172 ± 0.327
1.25AspAsn: 1.25 ± 0.351
4.53AspPro: 4.53 ± 0.624
2.421AspGln: 2.421 ± 0.358
3.593AspArg: 3.593 ± 0.476
2.656AspSer: 2.656 ± 0.385
3.202AspThr: 3.202 ± 0.448
4.53AspVal: 4.53 ± 0.483
1.328AspTrp: 1.328 ± 0.344
2.265AspTyr: 2.265 ± 0.426
0.0AspXaa: 0.0 ± 0.0
Glu
8.279GluAla: 8.279 ± 0.923
1.015GluCys: 1.015 ± 0.275
3.983GluAsp: 3.983 ± 0.73
3.905GluGlu: 3.905 ± 0.631
2.968GluPhe: 2.968 ± 0.462
6.092GluGly: 6.092 ± 0.894
1.328GluHis: 1.328 ± 0.338
1.953GluIle: 1.953 ± 0.348
1.953GluLys: 1.953 ± 0.377
5.389GluLeu: 5.389 ± 0.691
0.625GluMet: 0.625 ± 0.193
1.718GluAsn: 1.718 ± 0.418
2.187GluPro: 2.187 ± 0.395
1.093GluGln: 1.093 ± 0.237
5.311GluArg: 5.311 ± 0.779
2.421GluSer: 2.421 ± 0.424
4.686GluThr: 4.686 ± 0.709
4.296GluVal: 4.296 ± 0.734
1.64GluTrp: 1.64 ± 0.33
1.64GluTyr: 1.64 ± 0.357
0.0GluXaa: 0.0 ± 0.0
Phe
3.28PheAla: 3.28 ± 0.578
0.312PheCys: 0.312 ± 0.148
2.421PheAsp: 2.421 ± 0.392
1.796PheGlu: 1.796 ± 0.322
0.703PhePhe: 0.703 ± 0.198
3.437PheGly: 3.437 ± 0.522
0.391PheHis: 0.391 ± 0.151
1.093PheIle: 1.093 ± 0.288
1.328PheLys: 1.328 ± 0.316
1.796PheLeu: 1.796 ± 0.352
0.703PheMet: 0.703 ± 0.264
1.093PheAsn: 1.093 ± 0.286
1.484PhePro: 1.484 ± 0.395
1.015PheGln: 1.015 ± 0.279
2.656PheArg: 2.656 ± 0.47
1.172PheSer: 1.172 ± 0.302
1.406PheThr: 1.406 ± 0.464
2.031PheVal: 2.031 ± 0.419
0.391PheTrp: 0.391 ± 0.181
0.937PheTyr: 0.937 ± 0.293
0.0PheXaa: 0.0 ± 0.0
Gly
9.138GlyAla: 9.138 ± 1.222
0.703GlyCys: 0.703 ± 0.205
5.467GlyAsp: 5.467 ± 0.644
5.467GlyGlu: 5.467 ± 0.856
3.359GlyPhe: 3.359 ± 0.682
6.717GlyGly: 6.717 ± 0.963
1.25GlyHis: 1.25 ± 0.317
3.593GlyIle: 3.593 ± 0.656
6.092GlyLys: 6.092 ± 0.734
6.561GlyLeu: 6.561 ± 0.887
2.968GlyMet: 2.968 ± 0.425
3.593GlyAsn: 3.593 ± 0.428
3.437GlyPro: 3.437 ± 0.604
2.343GlyGln: 2.343 ± 0.452
4.608GlyArg: 4.608 ± 0.698
4.296GlySer: 4.296 ± 0.625
6.17GlyThr: 6.17 ± 0.829
7.03GlyVal: 7.03 ± 0.883
2.187GlyTrp: 2.187 ± 0.4
3.437GlyTyr: 3.437 ± 0.758
0.0GlyXaa: 0.0 ± 0.0
His
1.718HisAla: 1.718 ± 0.32
0.156HisCys: 0.156 ± 0.097
1.25HisAsp: 1.25 ± 0.33
0.937HisGlu: 0.937 ± 0.234
0.469HisPhe: 0.469 ± 0.184
1.64HisGly: 1.64 ± 0.437
0.234HisHis: 0.234 ± 0.118
0.547HisIle: 0.547 ± 0.196
0.859HisLys: 0.859 ± 0.228
1.25HisLeu: 1.25 ± 0.306
0.234HisMet: 0.234 ± 0.127
0.547HisAsn: 0.547 ± 0.216
0.859HisPro: 0.859 ± 0.283
0.625HisGln: 0.625 ± 0.226
0.625HisArg: 0.625 ± 0.221
0.625HisSer: 0.625 ± 0.186
1.64HisThr: 1.64 ± 0.314
1.64HisVal: 1.64 ± 0.325
0.156HisTrp: 0.156 ± 0.112
0.391HisTyr: 0.391 ± 0.164
0.0HisXaa: 0.0 ± 0.0
Ile
4.14IleAla: 4.14 ± 0.658
0.156IleCys: 0.156 ± 0.102
2.187IleAsp: 2.187 ± 0.479
2.578IleGlu: 2.578 ± 0.511
1.015IlePhe: 1.015 ± 0.311
2.499IleGly: 2.499 ± 0.454
0.859IleHis: 0.859 ± 0.259
1.562IleIle: 1.562 ± 0.326
2.109IleLys: 2.109 ± 0.545
2.031IleLeu: 2.031 ± 0.34
0.469IleMet: 0.469 ± 0.214
1.015IleAsn: 1.015 ± 0.187
3.202IlePro: 3.202 ± 0.504
1.796IleGln: 1.796 ± 0.291
2.734IleArg: 2.734 ± 0.519
1.484IleSer: 1.484 ± 0.293
3.515IleThr: 3.515 ± 0.595
2.187IleVal: 2.187 ± 0.445
0.781IleTrp: 0.781 ± 0.206
0.781IleTyr: 0.781 ± 0.257
0.0IleXaa: 0.0 ± 0.0
Lys
7.264LysAla: 7.264 ± 0.832
0.469LysCys: 0.469 ± 0.169
2.109LysAsp: 2.109 ± 0.397
2.812LysGlu: 2.812 ± 0.552
1.328LysPhe: 1.328 ± 0.25
3.905LysGly: 3.905 ± 0.785
0.781LysHis: 0.781 ± 0.255
1.484LysIle: 1.484 ± 0.397
2.734LysLys: 2.734 ± 0.637
2.656LysLeu: 2.656 ± 0.398
0.937LysMet: 0.937 ± 0.297
0.781LysAsn: 0.781 ± 0.24
4.765LysPro: 4.765 ± 0.698
0.234LysGln: 0.234 ± 0.164
4.374LysArg: 4.374 ± 0.659
2.187LysSer: 2.187 ± 0.406
3.124LysThr: 3.124 ± 0.424
3.827LysVal: 3.827 ± 0.628
0.625LysTrp: 0.625 ± 0.195
1.093LysTyr: 1.093 ± 0.27
0.0LysXaa: 0.0 ± 0.0
Leu
8.904LeuAla: 8.904 ± 0.786
1.093LeuCys: 1.093 ± 0.346
5.936LeuAsp: 5.936 ± 0.785
4.765LeuGlu: 4.765 ± 0.678
2.499LeuPhe: 2.499 ± 0.389
5.624LeuGly: 5.624 ± 0.745
1.25LeuHis: 1.25 ± 0.395
3.124LeuIle: 3.124 ± 0.513
4.218LeuLys: 4.218 ± 0.432
4.686LeuLeu: 4.686 ± 0.741
2.109LeuMet: 2.109 ± 0.403
2.031LeuAsn: 2.031 ± 0.383
3.905LeuPro: 3.905 ± 0.609
2.343LeuGln: 2.343 ± 0.427
5.936LeuArg: 5.936 ± 0.725
5.311LeuSer: 5.311 ± 0.82
5.311LeuThr: 5.311 ± 0.545
6.014LeuVal: 6.014 ± 0.762
2.109LeuTrp: 2.109 ± 0.492
1.64LeuTyr: 1.64 ± 0.335
0.0LeuXaa: 0.0 ± 0.0
Met
3.28MetAla: 3.28 ± 0.384
0.234MetCys: 0.234 ± 0.128
1.25MetAsp: 1.25 ± 0.265
0.625MetGlu: 0.625 ± 0.205
0.469MetPhe: 0.469 ± 0.164
1.796MetGly: 1.796 ± 0.338
0.312MetHis: 0.312 ± 0.139
0.859MetIle: 0.859 ± 0.274
0.469MetLys: 0.469 ± 0.172
1.328MetLeu: 1.328 ± 0.335
0.156MetMet: 0.156 ± 0.098
0.703MetAsn: 0.703 ± 0.217
1.64MetPro: 1.64 ± 0.326
1.172MetGln: 1.172 ± 0.29
1.718MetArg: 1.718 ± 0.357
1.796MetSer: 1.796 ± 0.342
1.562MetThr: 1.562 ± 0.286
2.109MetVal: 2.109 ± 0.301
0.391MetTrp: 0.391 ± 0.15
0.078MetTyr: 0.078 ± 0.084
0.0MetXaa: 0.0 ± 0.0
Asn
4.843AsnAla: 4.843 ± 0.784
0.156AsnCys: 0.156 ± 0.108
1.406AsnAsp: 1.406 ± 0.317
1.562AsnGlu: 1.562 ± 0.282
0.547AsnPhe: 0.547 ± 0.193
4.218AsnGly: 4.218 ± 0.776
0.391AsnHis: 0.391 ± 0.153
1.093AsnIle: 1.093 ± 0.267
1.406AsnLys: 1.406 ± 0.464
1.953AsnLeu: 1.953 ± 0.535
0.391AsnMet: 0.391 ± 0.168
0.547AsnAsn: 0.547 ± 0.248
2.656AsnPro: 2.656 ± 0.364
0.625AsnGln: 0.625 ± 0.243
2.187AsnArg: 2.187 ± 0.328
0.781AsnSer: 0.781 ± 0.253
1.953AsnThr: 1.953 ± 0.375
2.343AsnVal: 2.343 ± 0.421
0.469AsnTrp: 0.469 ± 0.155
0.859AsnTyr: 0.859 ± 0.237
0.0AsnXaa: 0.0 ± 0.0
Pro
5.78ProAla: 5.78 ± 0.794
0.625ProCys: 0.625 ± 0.295
3.827ProAsp: 3.827 ± 0.615
4.921ProGlu: 4.921 ± 0.621
1.562ProPhe: 1.562 ± 0.373
5.389ProGly: 5.389 ± 0.601
0.937ProHis: 0.937 ± 0.306
1.562ProIle: 1.562 ± 0.401
3.046ProLys: 3.046 ± 0.647
4.374ProLeu: 4.374 ± 0.734
1.406ProMet: 1.406 ± 0.266
2.499ProAsn: 2.499 ± 0.49
1.796ProPro: 1.796 ± 0.502
1.093ProGln: 1.093 ± 0.246
2.578ProArg: 2.578 ± 0.475
2.187ProSer: 2.187 ± 0.414
2.656ProThr: 2.656 ± 0.528
4.452ProVal: 4.452 ± 0.734
0.625ProTrp: 0.625 ± 0.202
1.875ProTyr: 1.875 ± 0.403
0.0ProXaa: 0.0 ± 0.0
Gln
3.749GlnAla: 3.749 ± 0.543
0.312GlnCys: 0.312 ± 0.134
1.406GlnAsp: 1.406 ± 0.301
1.25GlnGlu: 1.25 ± 0.302
0.547GlnPhe: 0.547 ± 0.162
3.124GlnGly: 3.124 ± 0.615
0.391GlnHis: 0.391 ± 0.183
0.781GlnIle: 0.781 ± 0.317
0.625GlnLys: 0.625 ± 0.251
2.031GlnLeu: 2.031 ± 0.346
0.547GlnMet: 0.547 ± 0.199
0.781GlnAsn: 0.781 ± 0.195
1.406GlnPro: 1.406 ± 0.372
0.469GlnGln: 0.469 ± 0.218
3.046GlnArg: 3.046 ± 0.443
1.093GlnSer: 1.093 ± 0.294
2.89GlnThr: 2.89 ± 0.4
1.875GlnVal: 1.875 ± 0.337
0.781GlnTrp: 0.781 ± 0.208
0.781GlnTyr: 0.781 ± 0.227
0.0GlnXaa: 0.0 ± 0.0
Arg
6.483ArgAla: 6.483 ± 0.749
1.172ArgCys: 1.172 ± 0.372
3.359ArgAsp: 3.359 ± 0.38
4.374ArgGlu: 4.374 ± 0.689
1.796ArgPhe: 1.796 ± 0.391
6.092ArgGly: 6.092 ± 0.669
1.718ArgHis: 1.718 ± 0.392
2.734ArgIle: 2.734 ± 0.458
3.046ArgLys: 3.046 ± 0.43
7.342ArgLeu: 7.342 ± 0.949
2.499ArgMet: 2.499 ± 0.388
1.328ArgAsn: 1.328 ± 0.318
2.656ArgPro: 2.656 ± 0.498
2.89ArgGln: 2.89 ± 0.439
5.389ArgArg: 5.389 ± 0.981
3.515ArgSer: 3.515 ± 0.523
3.749ArgThr: 3.749 ± 0.585
5.936ArgVal: 5.936 ± 0.644
1.64ArgTrp: 1.64 ± 0.341
1.953ArgTyr: 1.953 ± 0.396
0.0ArgXaa: 0.0 ± 0.0
Ser
6.717SerAla: 6.717 ± 0.663
0.547SerCys: 0.547 ± 0.199
2.578SerAsp: 2.578 ± 0.386
3.905SerGlu: 3.905 ± 0.647
1.406SerPhe: 1.406 ± 0.431
4.843SerGly: 4.843 ± 0.691
0.312SerHis: 0.312 ± 0.141
1.953SerIle: 1.953 ± 0.361
2.187SerLys: 2.187 ± 0.342
4.062SerLeu: 4.062 ± 0.497
0.937SerMet: 0.937 ± 0.277
1.796SerAsn: 1.796 ± 0.37
2.031SerPro: 2.031 ± 0.396
1.25SerGln: 1.25 ± 0.311
2.968SerArg: 2.968 ± 0.325
2.968SerSer: 2.968 ± 0.653
3.359SerThr: 3.359 ± 0.543
5.077SerVal: 5.077 ± 0.803
1.015SerTrp: 1.015 ± 0.293
1.172SerTyr: 1.172 ± 0.298
0.0SerXaa: 0.0 ± 0.0
Thr
7.733ThrAla: 7.733 ± 0.846
0.703ThrCys: 0.703 ± 0.239
4.062ThrAsp: 4.062 ± 0.604
4.296ThrGlu: 4.296 ± 0.721
2.343ThrPhe: 2.343 ± 0.43
6.483ThrGly: 6.483 ± 0.655
1.328ThrHis: 1.328 ± 0.353
2.812ThrIle: 2.812 ± 0.521
2.968ThrLys: 2.968 ± 0.424
5.233ThrLeu: 5.233 ± 0.726
1.015ThrMet: 1.015 ± 0.238
2.343ThrAsn: 2.343 ± 0.415
4.218ThrPro: 4.218 ± 0.743
1.328ThrGln: 1.328 ± 0.274
3.983ThrArg: 3.983 ± 0.55
3.359ThrSer: 3.359 ± 0.756
5.077ThrThr: 5.077 ± 0.809
5.311ThrVal: 5.311 ± 0.8
1.562ThrTrp: 1.562 ± 0.364
1.875ThrTyr: 1.875 ± 0.298
0.0ThrXaa: 0.0 ± 0.0
Val
6.873ValAla: 6.873 ± 0.706
0.391ValCys: 0.391 ± 0.166
5.624ValAsp: 5.624 ± 0.816
4.218ValGlu: 4.218 ± 0.543
2.031ValPhe: 2.031 ± 0.41
5.546ValGly: 5.546 ± 0.855
1.25ValHis: 1.25 ± 0.305
3.124ValIle: 3.124 ± 0.481
3.671ValLys: 3.671 ± 0.495
6.561ValLeu: 6.561 ± 0.568
1.562ValMet: 1.562 ± 0.367
2.734ValAsn: 2.734 ± 0.37
4.843ValPro: 4.843 ± 0.649
1.64ValGln: 1.64 ± 0.338
6.17ValArg: 6.17 ± 0.837
5.702ValSer: 5.702 ± 0.839
6.639ValThr: 6.639 ± 0.94
4.999ValVal: 4.999 ± 0.659
1.796ValTrp: 1.796 ± 0.319
2.812ValTyr: 2.812 ± 0.475
0.0ValXaa: 0.0 ± 0.0
Trp
1.953TrpAla: 1.953 ± 0.301
0.391TrpCys: 0.391 ± 0.203
1.406TrpAsp: 1.406 ± 0.329
0.703TrpGlu: 0.703 ± 0.189
0.781TrpPhe: 0.781 ± 0.257
1.093TrpGly: 1.093 ± 0.251
0.781TrpHis: 0.781 ± 0.252
0.703TrpIle: 0.703 ± 0.272
0.937TrpLys: 0.937 ± 0.219
1.953TrpLeu: 1.953 ± 0.379
0.312TrpMet: 0.312 ± 0.148
0.625TrpAsn: 0.625 ± 0.252
0.391TrpPro: 0.391 ± 0.167
0.781TrpGln: 0.781 ± 0.309
1.64TrpArg: 1.64 ± 0.313
1.562TrpSer: 1.562 ± 0.28
1.484TrpThr: 1.484 ± 0.353
1.953TrpVal: 1.953 ± 0.415
0.156TrpTrp: 0.156 ± 0.154
0.625TrpTyr: 0.625 ± 0.318
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.671TyrAla: 3.671 ± 0.601
0.391TyrCys: 0.391 ± 0.171
1.796TyrAsp: 1.796 ± 0.354
1.328TyrGlu: 1.328 ± 0.315
0.781TyrPhe: 0.781 ± 0.178
3.749TyrGly: 3.749 ± 0.554
0.312TyrHis: 0.312 ± 0.152
0.781TyrIle: 0.781 ± 0.227
1.015TyrLys: 1.015 ± 0.254
1.875TyrLeu: 1.875 ± 0.353
0.625TyrMet: 0.625 ± 0.211
1.406TyrAsn: 1.406 ± 0.301
1.718TyrPro: 1.718 ± 0.538
0.547TyrGln: 0.547 ± 0.182
1.875TyrArg: 1.875 ± 0.31
1.64TyrSer: 1.64 ± 0.317
1.64TyrThr: 1.64 ± 0.325
2.499TyrVal: 2.499 ± 0.432
0.156TyrTrp: 0.156 ± 0.116
0.391TyrTyr: 0.391 ± 0.149
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (12804 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski