Amino acid dipepetide frequency for Gordonia phage Wizard

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.556AlaAla: 17.556 ± 1.718
1.08AlaCys: 1.08 ± 0.249
8.751AlaAsp: 8.751 ± 0.739
8.751AlaGlu: 8.751 ± 0.804
3.079AlaPhe: 3.079 ± 0.4
9.777AlaGly: 9.777 ± 0.871
2.377AlaHis: 2.377 ± 0.478
5.726AlaIle: 5.726 ± 0.524
3.727AlaLys: 3.727 ± 0.531
10.804AlaLeu: 10.804 ± 1.087
3.241AlaMet: 3.241 ± 0.299
3.349AlaAsn: 3.349 ± 0.486
6.32AlaPro: 6.32 ± 0.751
4.051AlaGln: 4.051 ± 0.673
8.859AlaArg: 8.859 ± 0.718
5.402AlaSer: 5.402 ± 0.612
7.509AlaThr: 7.509 ± 0.885
8.157AlaVal: 8.157 ± 0.757
2.701AlaTrp: 2.701 ± 0.329
2.485AlaTyr: 2.485 ± 0.352
0.0AlaXaa: 0.0 ± 0.0
Cys
0.972CysAla: 0.972 ± 0.226
0.162CysCys: 0.162 ± 0.101
0.864CysAsp: 0.864 ± 0.274
0.432CysGlu: 0.432 ± 0.163
0.162CysPhe: 0.162 ± 0.09
1.134CysGly: 1.134 ± 0.309
0.216CysHis: 0.216 ± 0.103
0.0CysIle: 0.0 ± 0.0
0.108CysLys: 0.108 ± 0.078
0.216CysLeu: 0.216 ± 0.107
0.054CysMet: 0.054 ± 0.062
0.27CysAsn: 0.27 ± 0.117
0.972CysPro: 0.972 ± 0.262
0.108CysGln: 0.108 ± 0.077
0.81CysArg: 0.81 ± 0.237
0.702CysSer: 0.702 ± 0.214
0.648CysThr: 0.648 ± 0.191
0.378CysVal: 0.378 ± 0.145
0.0CysTrp: 0.0 ± 0.0
0.108CysTyr: 0.108 ± 0.091
0.0CysXaa: 0.0 ± 0.0
Asp
8.265AspAla: 8.265 ± 0.71
0.486AspCys: 0.486 ± 0.173
5.51AspAsp: 5.51 ± 0.763
5.132AspGlu: 5.132 ± 0.757
1.675AspPhe: 1.675 ± 0.278
5.888AspGly: 5.888 ± 0.611
1.891AspHis: 1.891 ± 0.364
1.945AspIle: 1.945 ± 0.413
1.134AspLys: 1.134 ± 0.275
6.428AspLeu: 6.428 ± 0.506
1.242AspMet: 1.242 ± 0.267
1.945AspAsn: 1.945 ± 0.418
5.996AspPro: 5.996 ± 0.644
2.431AspGln: 2.431 ± 0.341
4.646AspArg: 4.646 ± 0.79
3.187AspSer: 3.187 ± 0.466
4.43AspThr: 4.43 ± 0.542
5.726AspVal: 5.726 ± 0.604
1.621AspTrp: 1.621 ± 0.277
2.053AspTyr: 2.053 ± 0.409
0.0AspXaa: 0.0 ± 0.0
Glu
6.482GluAla: 6.482 ± 0.7
0.702GluCys: 0.702 ± 0.235
3.025GluAsp: 3.025 ± 0.398
2.755GluGlu: 2.755 ± 0.468
1.729GluPhe: 1.729 ± 0.335
3.943GluGly: 3.943 ± 0.793
1.026GluHis: 1.026 ± 0.257
2.863GluIle: 2.863 ± 0.427
1.999GluLys: 1.999 ± 0.373
5.834GluLeu: 5.834 ± 0.889
0.81GluMet: 0.81 ± 0.186
1.567GluAsn: 1.567 ± 0.288
3.727GluPro: 3.727 ± 0.671
2.701GluGln: 2.701 ± 0.468
3.673GluArg: 3.673 ± 0.509
2.809GluSer: 2.809 ± 0.324
2.809GluThr: 2.809 ± 0.436
4.538GluVal: 4.538 ± 0.445
2.053GluTrp: 2.053 ± 0.408
1.296GluTyr: 1.296 ± 0.242
0.0GluXaa: 0.0 ± 0.0
Phe
3.025PheAla: 3.025 ± 0.361
0.108PheCys: 0.108 ± 0.065
2.107PheAsp: 2.107 ± 0.298
1.621PheGlu: 1.621 ± 0.33
1.08PhePhe: 1.08 ± 0.318
1.837PheGly: 1.837 ± 0.357
0.702PheHis: 0.702 ± 0.251
0.972PheIle: 0.972 ± 0.23
0.594PheLys: 0.594 ± 0.2
1.404PheLeu: 1.404 ± 0.276
0.378PheMet: 0.378 ± 0.138
0.918PheAsn: 0.918 ± 0.388
1.134PhePro: 1.134 ± 0.268
0.81PheGln: 0.81 ± 0.223
2.107PheArg: 2.107 ± 0.307
1.513PheSer: 1.513 ± 0.316
2.053PheThr: 2.053 ± 0.383
1.837PheVal: 1.837 ± 0.32
0.432PheTrp: 0.432 ± 0.136
0.216PheTyr: 0.216 ± 0.114
0.0PheXaa: 0.0 ± 0.0
Gly
8.427GlyAla: 8.427 ± 0.921
0.756GlyCys: 0.756 ± 0.248
5.834GlyAsp: 5.834 ± 0.573
4.322GlyGlu: 4.322 ± 0.496
2.539GlyPhe: 2.539 ± 0.402
9.507GlyGly: 9.507 ± 1.709
2.053GlyHis: 2.053 ± 0.411
4.484GlyIle: 4.484 ± 0.404
3.187GlyLys: 3.187 ± 0.508
5.942GlyLeu: 5.942 ± 0.579
2.053GlyMet: 2.053 ± 0.346
3.079GlyAsn: 3.079 ± 0.487
4.376GlyPro: 4.376 ± 0.439
3.403GlyGln: 3.403 ± 0.475
5.996GlyArg: 5.996 ± 0.478
4.376GlySer: 4.376 ± 0.755
5.294GlyThr: 5.294 ± 0.94
7.617GlyVal: 7.617 ± 0.588
1.35GlyTrp: 1.35 ± 0.234
1.837GlyTyr: 1.837 ± 0.311
0.0GlyXaa: 0.0 ± 0.0
His
1.999HisAla: 1.999 ± 0.321
0.216HisCys: 0.216 ± 0.108
1.567HisAsp: 1.567 ± 0.363
1.08HisGlu: 1.08 ± 0.223
0.432HisPhe: 0.432 ± 0.146
1.513HisGly: 1.513 ± 0.253
0.81HisHis: 0.81 ± 0.277
1.188HisIle: 1.188 ± 0.229
0.27HisLys: 0.27 ± 0.105
1.891HisLeu: 1.891 ± 0.337
0.27HisMet: 0.27 ± 0.116
0.648HisAsn: 0.648 ± 0.219
1.891HisPro: 1.891 ± 0.403
0.918HisGln: 0.918 ± 0.287
1.999HisArg: 1.999 ± 0.431
0.81HisSer: 0.81 ± 0.215
1.621HisThr: 1.621 ± 0.31
1.134HisVal: 1.134 ± 0.299
0.702HisTrp: 0.702 ± 0.177
0.54HisTyr: 0.54 ± 0.195
0.0HisXaa: 0.0 ± 0.0
Ile
5.618IleAla: 5.618 ± 0.585
0.216IleCys: 0.216 ± 0.105
3.673IleAsp: 3.673 ± 0.499
2.809IleGlu: 2.809 ± 0.509
0.81IlePhe: 0.81 ± 0.245
4.268IleGly: 4.268 ± 0.654
0.702IleHis: 0.702 ± 0.204
1.567IleIle: 1.567 ± 0.37
0.864IleLys: 0.864 ± 0.235
2.863IleLeu: 2.863 ± 0.449
0.486IleMet: 0.486 ± 0.172
0.864IleAsn: 0.864 ± 0.194
2.809IlePro: 2.809 ± 0.325
1.134IleGln: 1.134 ± 0.232
3.511IleArg: 3.511 ± 0.617
1.891IleSer: 1.891 ± 0.285
3.079IleThr: 3.079 ± 0.42
3.457IleVal: 3.457 ± 0.467
0.594IleTrp: 0.594 ± 0.171
0.81IleTyr: 0.81 ± 0.246
0.0IleXaa: 0.0 ± 0.0
Lys
3.619LysAla: 3.619 ± 0.493
0.162LysCys: 0.162 ± 0.097
1.729LysAsp: 1.729 ± 0.368
1.35LysGlu: 1.35 ± 0.391
0.81LysPhe: 0.81 ± 0.234
1.945LysGly: 1.945 ± 0.346
0.27LysHis: 0.27 ± 0.114
0.972LysIle: 0.972 ± 0.195
1.242LysLys: 1.242 ± 0.317
3.025LysLeu: 3.025 ± 0.356
0.432LysMet: 0.432 ± 0.13
0.864LysAsn: 0.864 ± 0.205
1.729LysPro: 1.729 ± 0.306
0.756LysGln: 0.756 ± 0.227
2.539LysArg: 2.539 ± 0.325
1.513LysSer: 1.513 ± 0.28
2.323LysThr: 2.323 ± 0.384
2.107LysVal: 2.107 ± 0.292
0.432LysTrp: 0.432 ± 0.141
0.432LysTyr: 0.432 ± 0.143
0.0LysXaa: 0.0 ± 0.0
Leu
10.318LeuAla: 10.318 ± 0.849
0.54LeuCys: 0.54 ± 0.154
6.482LeuAsp: 6.482 ± 0.709
4.268LeuGlu: 4.268 ± 0.551
1.621LeuPhe: 1.621 ± 0.314
7.185LeuGly: 7.185 ± 1.004
1.404LeuHis: 1.404 ± 0.298
3.349LeuIle: 3.349 ± 0.425
1.945LeuLys: 1.945 ± 0.353
5.672LeuLeu: 5.672 ± 0.521
1.026LeuMet: 1.026 ± 0.235
1.621LeuAsn: 1.621 ± 0.293
4.213LeuPro: 4.213 ± 0.425
2.971LeuGln: 2.971 ± 0.471
5.834LeuArg: 5.834 ± 0.591
4.268LeuSer: 4.268 ± 0.527
5.942LeuThr: 5.942 ± 0.468
5.726LeuVal: 5.726 ± 0.617
2.215LeuTrp: 2.215 ± 0.349
1.729LeuTyr: 1.729 ± 0.286
0.0LeuXaa: 0.0 ± 0.0
Met
2.431MetAla: 2.431 ± 0.352
0.27MetCys: 0.27 ± 0.121
0.81MetAsp: 0.81 ± 0.22
0.54MetGlu: 0.54 ± 0.185
0.432MetPhe: 0.432 ± 0.168
1.567MetGly: 1.567 ± 0.234
0.27MetHis: 0.27 ± 0.11
0.702MetIle: 0.702 ± 0.164
0.594MetLys: 0.594 ± 0.146
1.242MetLeu: 1.242 ± 0.227
0.27MetMet: 0.27 ± 0.126
0.702MetAsn: 0.702 ± 0.173
1.513MetPro: 1.513 ± 0.271
0.81MetGln: 0.81 ± 0.232
1.404MetArg: 1.404 ± 0.239
1.675MetSer: 1.675 ± 0.279
3.079MetThr: 3.079 ± 0.445
1.35MetVal: 1.35 ± 0.277
0.432MetTrp: 0.432 ± 0.142
0.594MetTyr: 0.594 ± 0.192
0.0MetXaa: 0.0 ± 0.0
Asn
3.781AsnAla: 3.781 ± 0.492
0.324AsnCys: 0.324 ± 0.136
1.404AsnAsp: 1.404 ± 0.294
1.404AsnGlu: 1.404 ± 0.269
0.594AsnPhe: 0.594 ± 0.191
2.755AsnGly: 2.755 ± 0.363
0.81AsnHis: 0.81 ± 0.277
0.864AsnIle: 0.864 ± 0.207
0.864AsnLys: 0.864 ± 0.225
2.269AsnLeu: 2.269 ± 0.481
0.648AsnMet: 0.648 ± 0.159
0.594AsnAsn: 0.594 ± 0.162
2.593AsnPro: 2.593 ± 0.315
0.648AsnGln: 0.648 ± 0.243
2.053AsnArg: 2.053 ± 0.322
1.242AsnSer: 1.242 ± 0.27
1.945AsnThr: 1.945 ± 0.391
2.269AsnVal: 2.269 ± 0.447
0.378AsnTrp: 0.378 ± 0.135
0.486AsnTyr: 0.486 ± 0.18
0.0AsnXaa: 0.0 ± 0.0
Pro
8.265ProAla: 8.265 ± 0.785
0.486ProCys: 0.486 ± 0.172
5.996ProAsp: 5.996 ± 0.739
3.997ProGlu: 3.997 ± 0.608
1.35ProPhe: 1.35 ± 0.288
5.456ProGly: 5.456 ± 0.611
1.242ProHis: 1.242 ± 0.358
3.025ProIle: 3.025 ± 0.324
1.621ProLys: 1.621 ± 0.36
3.565ProLeu: 3.565 ± 0.483
0.972ProMet: 0.972 ± 0.204
1.459ProAsn: 1.459 ± 0.251
4.376ProPro: 4.376 ± 0.685
1.837ProGln: 1.837 ± 0.327
3.673ProArg: 3.673 ± 0.436
3.403ProSer: 3.403 ± 0.504
4.97ProThr: 4.97 ± 0.469
5.186ProVal: 5.186 ± 0.523
1.08ProTrp: 1.08 ± 0.285
0.972ProTyr: 0.972 ± 0.205
0.0ProXaa: 0.0 ± 0.0
Gln
4.268GlnAla: 4.268 ± 0.529
0.378GlnCys: 0.378 ± 0.143
1.567GlnAsp: 1.567 ± 0.292
1.513GlnGlu: 1.513 ± 0.343
0.864GlnPhe: 0.864 ± 0.193
2.053GlnGly: 2.053 ± 0.414
0.918GlnHis: 0.918 ± 0.227
1.675GlnIle: 1.675 ± 0.272
1.026GlnLys: 1.026 ± 0.264
2.917GlnLeu: 2.917 ± 0.373
1.242GlnMet: 1.242 ± 0.283
0.81GlnAsn: 0.81 ± 0.215
1.945GlnPro: 1.945 ± 0.303
1.567GlnGln: 1.567 ± 0.289
2.917GlnArg: 2.917 ± 0.41
1.945GlnSer: 1.945 ± 0.314
2.107GlnThr: 2.107 ± 0.386
2.647GlnVal: 2.647 ± 0.325
0.648GlnTrp: 0.648 ± 0.195
0.54GlnTyr: 0.54 ± 0.145
0.0GlnXaa: 0.0 ± 0.0
Arg
9.507ArgAla: 9.507 ± 0.873
0.81ArgCys: 0.81 ± 0.189
5.51ArgAsp: 5.51 ± 0.588
3.835ArgGlu: 3.835 ± 0.493
1.729ArgPhe: 1.729 ± 0.34
5.834ArgGly: 5.834 ± 0.569
1.945ArgHis: 1.945 ± 0.413
2.755ArgIle: 2.755 ± 0.367
3.187ArgLys: 3.187 ± 0.422
4.916ArgLeu: 4.916 ± 0.581
2.269ArgMet: 2.269 ± 0.361
1.999ArgAsn: 1.999 ± 0.327
3.511ArgPro: 3.511 ± 0.509
2.647ArgGln: 2.647 ± 0.445
7.185ArgArg: 7.185 ± 0.699
3.241ArgSer: 3.241 ± 0.454
4.159ArgThr: 4.159 ± 0.56
4.268ArgVal: 4.268 ± 0.667
1.999ArgTrp: 1.999 ± 0.373
1.675ArgTyr: 1.675 ± 0.357
0.0ArgXaa: 0.0 ± 0.0
Ser
6.752SerAla: 6.752 ± 0.733
0.27SerCys: 0.27 ± 0.136
3.889SerAsp: 3.889 ± 0.47
2.053SerGlu: 2.053 ± 0.266
1.134SerPhe: 1.134 ± 0.205
5.564SerGly: 5.564 ± 0.844
0.864SerHis: 0.864 ± 0.209
2.107SerIle: 2.107 ± 0.293
1.296SerLys: 1.296 ± 0.352
4.213SerLeu: 4.213 ± 0.362
1.242SerMet: 1.242 ± 0.259
1.35SerAsn: 1.35 ± 0.318
2.701SerPro: 2.701 ± 0.346
1.35SerGln: 1.35 ± 0.237
3.187SerArg: 3.187 ± 0.429
2.701SerSer: 2.701 ± 0.564
5.132SerThr: 5.132 ± 0.66
3.241SerVal: 3.241 ± 0.404
1.242SerTrp: 1.242 ± 0.281
1.188SerTyr: 1.188 ± 0.258
0.0SerXaa: 0.0 ± 0.0
Thr
9.939ThrAla: 9.939 ± 1.542
0.216ThrCys: 0.216 ± 0.113
4.484ThrAsp: 4.484 ± 0.445
3.997ThrGlu: 3.997 ± 0.449
1.783ThrPhe: 1.783 ± 0.263
6.644ThrGly: 6.644 ± 0.776
1.134ThrHis: 1.134 ± 0.228
3.565ThrIle: 3.565 ± 0.4
1.891ThrLys: 1.891 ± 0.259
4.754ThrLeu: 4.754 ± 0.523
1.837ThrMet: 1.837 ± 0.334
1.891ThrAsn: 1.891 ± 0.303
5.456ThrPro: 5.456 ± 0.393
1.513ThrGln: 1.513 ± 0.306
4.754ThrArg: 4.754 ± 0.448
3.835ThrSer: 3.835 ± 0.561
5.456ThrThr: 5.456 ± 0.624
5.564ThrVal: 5.564 ± 0.618
1.242ThrTrp: 1.242 ± 0.244
1.242ThrTyr: 1.242 ± 0.316
0.0ThrXaa: 0.0 ± 0.0
Val
8.049ValAla: 8.049 ± 0.642
0.702ValCys: 0.702 ± 0.195
5.456ValAsp: 5.456 ± 0.446
4.376ValGlu: 4.376 ± 0.445
1.729ValPhe: 1.729 ± 0.373
5.834ValGly: 5.834 ± 0.761
1.621ValHis: 1.621 ± 0.321
3.187ValIle: 3.187 ± 0.388
1.729ValLys: 1.729 ± 0.32
6.86ValLeu: 6.86 ± 0.694
1.242ValMet: 1.242 ± 0.249
2.485ValAsn: 2.485 ± 0.448
5.132ValPro: 5.132 ± 0.561
2.323ValGln: 2.323 ± 0.423
4.808ValArg: 4.808 ± 0.625
4.808ValSer: 4.808 ± 0.588
5.402ValThr: 5.402 ± 0.527
6.86ValVal: 6.86 ± 0.543
1.729ValTrp: 1.729 ± 0.35
1.296ValTyr: 1.296 ± 0.325
0.0ValXaa: 0.0 ± 0.0
Trp
2.539TrpAla: 2.539 ± 0.444
0.324TrpCys: 0.324 ± 0.136
1.296TrpAsp: 1.296 ± 0.246
0.864TrpGlu: 0.864 ± 0.199
0.594TrpPhe: 0.594 ± 0.215
1.242TrpGly: 1.242 ± 0.227
0.648TrpHis: 0.648 ± 0.186
0.918TrpIle: 0.918 ± 0.24
0.54TrpLys: 0.54 ± 0.17
2.215TrpLeu: 2.215 ± 0.328
0.54TrpMet: 0.54 ± 0.171
0.918TrpAsn: 0.918 ± 0.198
1.296TrpPro: 1.296 ± 0.291
1.08TrpGln: 1.08 ± 0.264
1.08TrpArg: 1.08 ± 0.251
1.296TrpSer: 1.296 ± 0.292
1.675TrpThr: 1.675 ± 0.277
1.945TrpVal: 1.945 ± 0.298
0.81TrpTrp: 0.81 ± 0.23
0.27TrpTyr: 0.27 ± 0.136
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.891TyrAla: 1.891 ± 0.373
0.108TyrCys: 0.108 ± 0.069
1.675TyrAsp: 1.675 ± 0.366
1.242TyrGlu: 1.242 ± 0.271
0.702TyrPhe: 0.702 ± 0.22
2.485TyrGly: 2.485 ± 0.404
0.594TyrHis: 0.594 ± 0.216
0.162TyrIle: 0.162 ± 0.091
0.432TyrLys: 0.432 ± 0.186
1.296TyrLeu: 1.296 ± 0.241
0.378TyrMet: 0.378 ± 0.122
0.594TyrAsn: 0.594 ± 0.168
1.242TyrPro: 1.242 ± 0.326
0.486TyrGln: 0.486 ± 0.164
1.945TyrArg: 1.945 ± 0.433
0.918TyrSer: 0.918 ± 0.181
1.567TyrThr: 1.567 ± 0.356
1.621TyrVal: 1.621 ± 0.265
0.432TyrTrp: 0.432 ± 0.145
0.378TyrTyr: 0.378 ± 0.155
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 89 proteins (18513 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski