Amino acid dipepetide frequency for Mycobacterium phage Chadwick

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.965AlaAla: 9.965 ± 0.974
0.459AlaCys: 0.459 ± 0.163
5.179AlaAsp: 5.179 ± 0.579
6.883AlaGlu: 6.883 ± 0.853
3.016AlaPhe: 3.016 ± 0.432
6.687AlaGly: 6.687 ± 0.62
1.639AlaHis: 1.639 ± 0.268
4.655AlaIle: 4.655 ± 0.617
4.065AlaLys: 4.065 ± 0.528
8.129AlaLeu: 8.129 ± 0.829
2.032AlaMet: 2.032 ± 0.386
2.884AlaAsn: 2.884 ± 0.443
4.261AlaPro: 4.261 ± 0.567
2.753AlaGln: 2.753 ± 0.445
5.638AlaArg: 5.638 ± 0.657
5.441AlaSer: 5.441 ± 0.703
4.786AlaThr: 4.786 ± 0.613
6.621AlaVal: 6.621 ± 0.877
1.442AlaTrp: 1.442 ± 0.288
1.639AlaTyr: 1.639 ± 0.327
0.0AlaXaa: 0.0 ± 0.0
Cys
0.59CysAla: 0.59 ± 0.203
0.066CysCys: 0.066 ± 0.071
0.524CysAsp: 0.524 ± 0.174
0.787CysGlu: 0.787 ± 0.207
0.393CysPhe: 0.393 ± 0.145
0.721CysGly: 0.721 ± 0.222
0.328CysHis: 0.328 ± 0.137
0.524CysIle: 0.524 ± 0.201
0.524CysLys: 0.524 ± 0.173
0.656CysLeu: 0.656 ± 0.216
0.131CysMet: 0.131 ± 0.095
0.459CysAsn: 0.459 ± 0.159
0.393CysPro: 0.393 ± 0.166
0.197CysGln: 0.197 ± 0.115
0.918CysArg: 0.918 ± 0.238
0.524CysSer: 0.524 ± 0.178
0.131CysThr: 0.131 ± 0.092
0.59CysVal: 0.59 ± 0.25
0.328CysTrp: 0.328 ± 0.184
0.197CysTyr: 0.197 ± 0.127
0.0CysXaa: 0.0 ± 0.0
Asp
6.293AspAla: 6.293 ± 0.694
0.721AspCys: 0.721 ± 0.205
3.147AspAsp: 3.147 ± 0.514
4.589AspGlu: 4.589 ± 0.585
2.557AspPhe: 2.557 ± 0.519
6.228AspGly: 6.228 ± 0.818
1.377AspHis: 1.377 ± 0.367
3.54AspIle: 3.54 ± 0.467
2.622AspLys: 2.622 ± 0.436
6.162AspLeu: 6.162 ± 0.497
1.442AspMet: 1.442 ± 0.268
2.098AspAsn: 2.098 ± 0.388
5.31AspPro: 5.31 ± 0.708
3.147AspGln: 3.147 ± 0.538
3.409AspArg: 3.409 ± 0.494
3.409AspSer: 3.409 ± 0.477
3.278AspThr: 3.278 ± 0.485
5.179AspVal: 5.179 ± 0.545
1.639AspTrp: 1.639 ± 0.349
3.212AspTyr: 3.212 ± 0.402
0.0AspXaa: 0.0 ± 0.0
Glu
6.228GluAla: 6.228 ± 0.723
0.656GluCys: 0.656 ± 0.211
5.441GluAsp: 5.441 ± 0.639
5.507GluGlu: 5.507 ± 0.713
2.688GluPhe: 2.688 ± 0.464
5.769GluGly: 5.769 ± 0.583
1.508GluHis: 1.508 ± 0.324
4.392GluIle: 4.392 ± 0.588
2.95GluLys: 2.95 ± 0.391
7.211GluLeu: 7.211 ± 0.745
2.032GluMet: 2.032 ± 0.302
2.294GluAsn: 2.294 ± 0.372
2.819GluPro: 2.819 ± 0.443
2.819GluGln: 2.819 ± 0.399
5.703GluArg: 5.703 ± 0.823
2.95GluSer: 2.95 ± 0.4
3.606GluThr: 3.606 ± 0.571
5.572GluVal: 5.572 ± 0.62
1.18GluTrp: 1.18 ± 0.279
2.36GluTyr: 2.36 ± 0.391
0.0GluXaa: 0.0 ± 0.0
Phe
2.622PheAla: 2.622 ± 0.439
0.131PheCys: 0.131 ± 0.094
3.081PheAsp: 3.081 ± 0.461
2.491PheGlu: 2.491 ± 0.355
0.787PhePhe: 0.787 ± 0.295
2.688PheGly: 2.688 ± 0.394
0.787PheHis: 0.787 ± 0.225
1.246PheIle: 1.246 ± 0.243
1.639PheLys: 1.639 ± 0.323
2.753PheLeu: 2.753 ± 0.504
0.459PheMet: 0.459 ± 0.173
1.508PheAsn: 1.508 ± 0.322
1.442PhePro: 1.442 ± 0.38
1.049PheGln: 1.049 ± 0.226
1.901PheArg: 1.901 ± 0.311
2.032PheSer: 2.032 ± 0.396
1.836PheThr: 1.836 ± 0.341
2.557PheVal: 2.557 ± 0.388
0.983PheTrp: 0.983 ± 0.272
1.442PheTyr: 1.442 ± 0.318
0.0PheXaa: 0.0 ± 0.0
Gly
5.835GlyAla: 5.835 ± 0.831
0.918GlyCys: 0.918 ± 0.283
5.572GlyAsp: 5.572 ± 0.776
5.31GlyGlu: 5.31 ± 0.713
2.098GlyPhe: 2.098 ± 0.465
9.506GlyGly: 9.506 ± 2.469
1.573GlyHis: 1.573 ± 0.318
4.655GlyIle: 4.655 ± 0.715
4.786GlyLys: 4.786 ± 0.623
6.425GlyLeu: 6.425 ± 0.837
2.098GlyMet: 2.098 ± 0.3
3.474GlyAsn: 3.474 ± 0.621
3.54GlyPro: 3.54 ± 0.516
2.95GlyGln: 2.95 ± 0.582
4.458GlyArg: 4.458 ± 0.463
4.589GlySer: 4.589 ± 0.659
4.523GlyThr: 4.523 ± 0.633
5.638GlyVal: 5.638 ± 0.585
2.032GlyTrp: 2.032 ± 0.369
2.557GlyTyr: 2.557 ± 0.379
0.0GlyXaa: 0.0 ± 0.0
His
1.442HisAla: 1.442 ± 0.341
0.197HisCys: 0.197 ± 0.107
1.442HisAsp: 1.442 ± 0.287
1.639HisGlu: 1.639 ± 0.32
0.524HisPhe: 0.524 ± 0.179
1.639HisGly: 1.639 ± 0.315
0.59HisHis: 0.59 ± 0.166
1.311HisIle: 1.311 ± 0.294
0.524HisLys: 0.524 ± 0.211
1.508HisLeu: 1.508 ± 0.356
0.197HisMet: 0.197 ± 0.134
0.59HisAsn: 0.59 ± 0.186
1.114HisPro: 1.114 ± 0.252
0.721HisGln: 0.721 ± 0.23
1.77HisArg: 1.77 ± 0.386
0.918HisSer: 0.918 ± 0.272
0.983HisThr: 0.983 ± 0.228
1.442HisVal: 1.442 ± 0.352
0.328HisTrp: 0.328 ± 0.189
0.59HisTyr: 0.59 ± 0.203
0.0HisXaa: 0.0 ± 0.0
Ile
5.835IleAla: 5.835 ± 0.712
0.524IleCys: 0.524 ± 0.171
4.261IleAsp: 4.261 ± 0.497
3.868IleGlu: 3.868 ± 0.528
1.049IlePhe: 1.049 ± 0.398
3.802IleGly: 3.802 ± 0.552
1.049IleHis: 1.049 ± 0.307
2.426IleIle: 2.426 ± 0.416
2.032IleLys: 2.032 ± 0.397
3.474IleLeu: 3.474 ± 0.472
0.656IleMet: 0.656 ± 0.183
2.229IleAsn: 2.229 ± 0.442
3.802IlePro: 3.802 ± 0.501
2.163IleGln: 2.163 ± 0.343
4.196IleArg: 4.196 ± 0.541
3.278IleSer: 3.278 ± 0.441
3.54IleThr: 3.54 ± 0.421
3.278IleVal: 3.278 ± 0.509
0.59IleTrp: 0.59 ± 0.189
1.508IleTyr: 1.508 ± 0.349
0.0IleXaa: 0.0 ± 0.0
Lys
5.048LysAla: 5.048 ± 0.658
0.328LysCys: 0.328 ± 0.135
2.819LysAsp: 2.819 ± 0.473
2.426LysGlu: 2.426 ± 0.485
1.442LysPhe: 1.442 ± 0.296
3.54LysGly: 3.54 ± 0.515
1.18LysHis: 1.18 ± 0.226
2.491LysIle: 2.491 ± 0.458
3.409LysLys: 3.409 ± 0.547
3.671LysLeu: 3.671 ± 0.482
1.246LysMet: 1.246 ± 0.3
1.114LysAsn: 1.114 ± 0.265
2.491LysPro: 2.491 ± 0.415
1.18LysGln: 1.18 ± 0.32
2.884LysArg: 2.884 ± 0.505
2.622LysSer: 2.622 ± 0.394
3.212LysThr: 3.212 ± 0.475
4.261LysVal: 4.261 ± 0.569
0.983LysTrp: 0.983 ± 0.241
1.049LysTyr: 1.049 ± 0.275
0.0LysXaa: 0.0 ± 0.0
Leu
7.605LeuAla: 7.605 ± 1.154
0.656LeuCys: 0.656 ± 0.213
6.031LeuAsp: 6.031 ± 0.733
5.9LeuGlu: 5.9 ± 0.712
2.229LeuPhe: 2.229 ± 0.367
5.835LeuGly: 5.835 ± 0.791
0.918LeuHis: 0.918 ± 0.289
3.868LeuIle: 3.868 ± 0.579
4.72LeuLys: 4.72 ± 0.734
5.835LeuLeu: 5.835 ± 0.559
2.163LeuMet: 2.163 ± 0.311
3.54LeuAsn: 3.54 ± 0.524
3.802LeuPro: 3.802 ± 0.544
2.032LeuGln: 2.032 ± 0.418
5.507LeuArg: 5.507 ± 0.542
6.228LeuSer: 6.228 ± 0.797
5.572LeuThr: 5.572 ± 0.63
6.359LeuVal: 6.359 ± 0.762
1.18LeuTrp: 1.18 ± 0.288
2.557LeuTyr: 2.557 ± 0.407
0.0LeuXaa: 0.0 ± 0.0
Met
1.704MetAla: 1.704 ± 0.327
0.262MetCys: 0.262 ± 0.127
1.311MetAsp: 1.311 ± 0.298
1.311MetGlu: 1.311 ± 0.313
0.983MetPhe: 0.983 ± 0.259
1.639MetGly: 1.639 ± 0.341
0.197MetHis: 0.197 ± 0.126
0.59MetIle: 0.59 ± 0.225
1.311MetLys: 1.311 ± 0.321
1.508MetLeu: 1.508 ± 0.347
0.393MetMet: 0.393 ± 0.184
0.787MetAsn: 0.787 ± 0.234
1.114MetPro: 1.114 ± 0.251
0.393MetGln: 0.393 ± 0.149
1.442MetArg: 1.442 ± 0.33
2.229MetSer: 2.229 ± 0.45
2.884MetThr: 2.884 ± 0.39
0.983MetVal: 0.983 ± 0.255
0.524MetTrp: 0.524 ± 0.171
0.787MetTyr: 0.787 ± 0.225
0.0MetXaa: 0.0 ± 0.0
Asn
2.819AsnAla: 2.819 ± 0.405
0.328AsnCys: 0.328 ± 0.188
2.163AsnAsp: 2.163 ± 0.461
1.967AsnGlu: 1.967 ± 0.344
1.77AsnPhe: 1.77 ± 0.4
4.851AsnGly: 4.851 ± 0.719
0.852AsnHis: 0.852 ± 0.219
1.508AsnIle: 1.508 ± 0.397
1.246AsnLys: 1.246 ± 0.313
2.884AsnLeu: 2.884 ± 0.369
0.459AsnMet: 0.459 ± 0.169
0.787AsnAsn: 0.787 ± 0.26
2.622AsnPro: 2.622 ± 0.488
1.18AsnGln: 1.18 ± 0.327
1.967AsnArg: 1.967 ± 0.466
1.704AsnSer: 1.704 ± 0.321
1.901AsnThr: 1.901 ± 0.515
2.229AsnVal: 2.229 ± 0.277
0.59AsnTrp: 0.59 ± 0.182
0.59AsnTyr: 0.59 ± 0.217
0.0AsnXaa: 0.0 ± 0.0
Pro
3.737ProAla: 3.737 ± 0.563
0.393ProCys: 0.393 ± 0.177
3.999ProAsp: 3.999 ± 0.517
4.523ProGlu: 4.523 ± 0.519
1.704ProPhe: 1.704 ± 0.447
4.982ProGly: 4.982 ± 0.916
0.787ProHis: 0.787 ± 0.21
2.819ProIle: 2.819 ± 0.381
2.294ProLys: 2.294 ± 0.425
3.278ProLeu: 3.278 ± 0.485
0.983ProMet: 0.983 ± 0.273
1.704ProAsn: 1.704 ± 0.319
3.212ProPro: 3.212 ± 0.904
1.442ProGln: 1.442 ± 0.31
2.032ProArg: 2.032 ± 0.372
3.147ProSer: 3.147 ± 0.44
3.54ProThr: 3.54 ± 0.448
4.13ProVal: 4.13 ± 0.492
1.18ProTrp: 1.18 ± 0.479
1.508ProTyr: 1.508 ± 0.319
0.0ProXaa: 0.0 ± 0.0
Gln
3.409GlnAla: 3.409 ± 0.399
0.262GlnCys: 0.262 ± 0.126
1.967GlnAsp: 1.967 ± 0.398
2.098GlnGlu: 2.098 ± 0.369
1.377GlnPhe: 1.377 ± 0.289
2.229GlnGly: 2.229 ± 0.499
0.721GlnHis: 0.721 ± 0.188
2.688GlnIle: 2.688 ± 0.438
1.901GlnLys: 1.901 ± 0.311
3.999GlnLeu: 3.999 ± 0.71
0.918GlnMet: 0.918 ± 0.233
0.721GlnAsn: 0.721 ± 0.235
1.114GlnPro: 1.114 ± 0.34
1.246GlnGln: 1.246 ± 0.306
1.508GlnArg: 1.508 ± 0.448
1.573GlnSer: 1.573 ± 0.34
1.836GlnThr: 1.836 ± 0.454
2.753GlnVal: 2.753 ± 0.436
0.524GlnTrp: 0.524 ± 0.183
0.918GlnTyr: 0.918 ± 0.224
0.0GlnXaa: 0.0 ± 0.0
Arg
4.851ArgAla: 4.851 ± 0.674
0.721ArgCys: 0.721 ± 0.289
3.409ArgAsp: 3.409 ± 0.399
5.507ArgGlu: 5.507 ± 0.665
2.688ArgPhe: 2.688 ± 0.441
4.13ArgGly: 4.13 ± 0.771
1.573ArgHis: 1.573 ± 0.364
3.868ArgIle: 3.868 ± 0.483
3.081ArgLys: 3.081 ± 0.528
6.162ArgLeu: 6.162 ± 0.537
1.901ArgMet: 1.901 ± 0.287
2.229ArgAsn: 2.229 ± 0.473
2.557ArgPro: 2.557 ± 0.502
2.491ArgGln: 2.491 ± 0.575
4.458ArgArg: 4.458 ± 0.621
2.229ArgSer: 2.229 ± 0.415
2.95ArgThr: 2.95 ± 0.401
3.606ArgVal: 3.606 ± 0.569
1.377ArgTrp: 1.377 ± 0.328
1.639ArgTyr: 1.639 ± 0.342
0.0ArgXaa: 0.0 ± 0.0
Ser
4.72SerAla: 4.72 ± 0.444
0.59SerCys: 0.59 ± 0.231
4.458SerAsp: 4.458 ± 0.558
4.72SerGlu: 4.72 ± 0.595
2.95SerPhe: 2.95 ± 0.387
4.72SerGly: 4.72 ± 0.569
0.983SerHis: 0.983 ± 0.244
3.343SerIle: 3.343 ± 0.428
2.819SerLys: 2.819 ± 0.377
3.868SerLeu: 3.868 ± 0.482
1.573SerMet: 1.573 ± 0.308
1.901SerAsn: 1.901 ± 0.368
2.557SerPro: 2.557 ± 0.414
2.032SerGln: 2.032 ± 0.374
4.13SerArg: 4.13 ± 0.491
3.343SerSer: 3.343 ± 0.455
2.491SerThr: 2.491 ± 0.395
3.343SerVal: 3.343 ± 0.455
1.18SerTrp: 1.18 ± 0.263
1.836SerTyr: 1.836 ± 0.361
0.0SerXaa: 0.0 ± 0.0
Thr
4.851ThrAla: 4.851 ± 0.486
0.459ThrCys: 0.459 ± 0.173
4.327ThrAsp: 4.327 ± 0.443
4.851ThrGlu: 4.851 ± 0.467
2.098ThrPhe: 2.098 ± 0.443
4.523ThrGly: 4.523 ± 0.624
1.246ThrHis: 1.246 ± 0.291
3.016ThrIle: 3.016 ± 0.428
2.426ThrLys: 2.426 ± 0.453
5.179ThrLeu: 5.179 ± 0.734
1.311ThrMet: 1.311 ± 0.297
1.967ThrAsn: 1.967 ± 0.329
4.065ThrPro: 4.065 ± 0.608
1.704ThrGln: 1.704 ± 0.361
2.819ThrArg: 2.819 ± 0.388
3.147ThrSer: 3.147 ± 0.501
3.671ThrThr: 3.671 ± 0.584
3.737ThrVal: 3.737 ± 0.476
1.246ThrTrp: 1.246 ± 0.302
1.442ThrTyr: 1.442 ± 0.261
0.0ThrXaa: 0.0 ± 0.0
Val
6.228ValAla: 6.228 ± 0.585
0.852ValCys: 0.852 ± 0.301
5.835ValAsp: 5.835 ± 0.815
5.769ValGlu: 5.769 ± 0.467
2.032ValPhe: 2.032 ± 0.378
4.851ValGly: 4.851 ± 0.497
1.311ValHis: 1.311 ± 0.304
3.54ValIle: 3.54 ± 0.415
3.409ValLys: 3.409 ± 0.44
5.245ValLeu: 5.245 ± 0.67
1.049ValMet: 1.049 ± 0.24
2.557ValAsn: 2.557 ± 0.488
3.016ValPro: 3.016 ± 0.442
2.294ValGln: 2.294 ± 0.488
3.474ValArg: 3.474 ± 0.509
5.376ValSer: 5.376 ± 0.542
4.589ValThr: 4.589 ± 0.479
6.359ValVal: 6.359 ± 0.805
1.836ValTrp: 1.836 ± 0.338
2.36ValTyr: 2.36 ± 0.393
0.0ValXaa: 0.0 ± 0.0
Trp
1.77TrpAla: 1.77 ± 0.306
0.197TrpCys: 0.197 ± 0.12
1.77TrpAsp: 1.77 ± 0.41
1.114TrpGlu: 1.114 ± 0.32
0.59TrpPhe: 0.59 ± 0.226
1.77TrpGly: 1.77 ± 0.317
0.459TrpHis: 0.459 ± 0.171
1.377TrpIle: 1.377 ± 0.319
0.656TrpLys: 0.656 ± 0.2
1.377TrpLeu: 1.377 ± 0.319
0.787TrpMet: 0.787 ± 0.192
0.787TrpAsn: 0.787 ± 0.253
0.721TrpPro: 0.721 ± 0.25
0.852TrpGln: 0.852 ± 0.171
0.852TrpArg: 0.852 ± 0.225
1.704TrpSer: 1.704 ± 0.31
1.311TrpThr: 1.311 ± 0.305
1.246TrpVal: 1.246 ± 0.296
0.459TrpTrp: 0.459 ± 0.194
0.459TrpTyr: 0.459 ± 0.175
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.622TyrAla: 2.622 ± 0.396
0.262TyrCys: 0.262 ± 0.138
2.622TyrAsp: 2.622 ± 0.549
2.426TyrGlu: 2.426 ± 0.504
0.459TyrPhe: 0.459 ± 0.161
2.426TyrGly: 2.426 ± 0.393
0.459TyrHis: 0.459 ± 0.159
1.704TyrIle: 1.704 ± 0.279
0.983TyrLys: 0.983 ± 0.225
3.212TyrLeu: 3.212 ± 0.388
0.459TyrMet: 0.459 ± 0.162
0.787TyrAsn: 0.787 ± 0.227
1.442TyrPro: 1.442 ± 0.314
1.114TyrGln: 1.114 ± 0.243
2.36TyrArg: 2.36 ± 0.4
1.114TyrSer: 1.114 ± 0.365
1.442TyrThr: 1.442 ± 0.28
2.098TyrVal: 2.098 ± 0.346
0.656TyrTrp: 0.656 ± 0.205
0.983TyrTyr: 0.983 ± 0.277
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 88 proteins (15255 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski