Amino acid dipepetide frequency for Staphylococcus phage phiSa2wa_st121mssa

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.627AlaAla: 2.627 ± 0.795
0.138AlaCys: 0.138 ± 0.105
2.696AlaAsp: 2.696 ± 0.492
3.802AlaGlu: 3.802 ± 0.426
1.59AlaPhe: 1.59 ± 0.322
3.733AlaGly: 3.733 ± 0.745
0.968AlaHis: 0.968 ± 0.256
4.701AlaIle: 4.701 ± 0.668
6.429AlaLys: 6.429 ± 1.121
4.977AlaLeu: 4.977 ± 0.733
1.244AlaMet: 1.244 ± 0.286
4.079AlaAsn: 4.079 ± 0.806
1.521AlaPro: 1.521 ± 0.358
1.728AlaGln: 1.728 ± 0.43
2.489AlaArg: 2.489 ± 0.381
4.493AlaSer: 4.493 ± 0.881
3.664AlaThr: 3.664 ± 0.511
2.765AlaVal: 2.765 ± 0.521
1.106AlaTrp: 1.106 ± 0.372
2.627AlaTyr: 2.627 ± 0.499
0.0AlaXaa: 0.0 ± 0.0
Cys
0.138CysAla: 0.138 ± 0.1
0.069CysCys: 0.069 ± 0.075
0.069CysAsp: 0.069 ± 0.057
0.484CysGlu: 0.484 ± 0.193
0.346CysPhe: 0.346 ± 0.157
0.138CysGly: 0.138 ± 0.096
0.138CysHis: 0.138 ± 0.127
0.622CysIle: 0.622 ± 0.208
0.484CysLys: 0.484 ± 0.191
0.691CysLeu: 0.691 ± 0.254
0.207CysMet: 0.207 ± 0.155
0.138CysAsn: 0.138 ± 0.109
0.069CysPro: 0.069 ± 0.063
0.138CysGln: 0.138 ± 0.117
0.346CysArg: 0.346 ± 0.162
0.207CysSer: 0.207 ± 0.122
0.207CysThr: 0.207 ± 0.132
0.207CysVal: 0.207 ± 0.122
0.0CysTrp: 0.0 ± 0.0
0.207CysTyr: 0.207 ± 0.134
0.0CysXaa: 0.0 ± 0.0
Asp
2.834AspAla: 2.834 ± 0.58
0.207AspCys: 0.207 ± 0.11
3.733AspAsp: 3.733 ± 0.583
4.77AspGlu: 4.77 ± 0.837
3.249AspPhe: 3.249 ± 0.517
3.387AspGly: 3.387 ± 0.579
0.76AspHis: 0.76 ± 0.211
5.323AspIle: 5.323 ± 0.595
6.982AspLys: 6.982 ± 0.728
5.807AspLeu: 5.807 ± 0.632
1.866AspMet: 1.866 ± 0.356
2.834AspAsn: 2.834 ± 0.431
1.175AspPro: 1.175 ± 0.351
1.175AspGln: 1.175 ± 0.222
2.005AspArg: 2.005 ± 0.352
3.802AspSer: 3.802 ± 0.623
3.733AspThr: 3.733 ± 0.528
3.94AspVal: 3.94 ± 0.456
0.899AspTrp: 0.899 ± 0.249
3.111AspTyr: 3.111 ± 0.596
0.0AspXaa: 0.0 ± 0.0
Glu
4.77GluAla: 4.77 ± 0.557
0.484GluCys: 0.484 ± 0.206
4.908GluAsp: 4.908 ± 0.785
6.775GluGlu: 6.775 ± 1.117
2.834GluPhe: 2.834 ± 0.448
3.387GluGly: 3.387 ± 0.497
1.106GluHis: 1.106 ± 0.247
5.185GluIle: 5.185 ± 0.877
8.848GluLys: 8.848 ± 0.922
7.051GluLeu: 7.051 ± 0.707
2.627GluMet: 2.627 ± 0.426
5.185GluAsn: 5.185 ± 0.637
1.175GluPro: 1.175 ± 0.307
3.111GluGln: 3.111 ± 0.565
2.834GluArg: 2.834 ± 0.496
3.526GluSer: 3.526 ± 0.45
3.733GluThr: 3.733 ± 0.642
4.079GluVal: 4.079 ± 0.501
0.899GluTrp: 0.899 ± 0.217
2.834GluTyr: 2.834 ± 0.621
0.0GluXaa: 0.0 ± 0.0
Phe
1.659PheAla: 1.659 ± 0.325
0.415PheCys: 0.415 ± 0.167
3.249PheAsp: 3.249 ± 0.418
2.903PheGlu: 2.903 ± 0.428
1.037PhePhe: 1.037 ± 0.247
2.972PheGly: 2.972 ± 0.519
0.622PheHis: 0.622 ± 0.179
3.664PheIle: 3.664 ± 0.639
4.217PheLys: 4.217 ± 0.58
2.627PheLeu: 2.627 ± 0.465
1.106PheMet: 1.106 ± 0.26
3.456PheAsn: 3.456 ± 0.478
0.83PhePro: 0.83 ± 0.275
1.175PheGln: 1.175 ± 0.223
1.106PheArg: 1.106 ± 0.242
2.074PheSer: 2.074 ± 0.382
2.005PheThr: 2.005 ± 0.389
2.074PheVal: 2.074 ± 0.451
0.346PheTrp: 0.346 ± 0.152
2.005PheTyr: 2.005 ± 0.423
0.0PheXaa: 0.0 ± 0.0
Gly
4.079GlyAla: 4.079 ± 1.036
0.277GlyCys: 0.277 ± 0.14
4.009GlyAsp: 4.009 ± 0.493
3.526GlyGlu: 3.526 ± 0.466
2.419GlyPhe: 2.419 ± 0.335
5.392GlyGly: 5.392 ± 1.158
1.383GlyHis: 1.383 ± 0.354
3.18GlyIle: 3.18 ± 0.491
5.668GlyLys: 5.668 ± 0.751
5.738GlyLeu: 5.738 ± 0.874
1.175GlyMet: 1.175 ± 0.364
3.664GlyAsn: 3.664 ± 0.583
1.037GlyPro: 1.037 ± 0.237
1.59GlyGln: 1.59 ± 0.378
2.074GlyArg: 2.074 ± 0.462
3.871GlySer: 3.871 ± 0.547
3.456GlyThr: 3.456 ± 0.565
4.701GlyVal: 4.701 ± 0.667
0.968GlyTrp: 0.968 ± 0.294
2.627GlyTyr: 2.627 ± 0.488
0.0GlyXaa: 0.0 ± 0.0
His
0.968HisAla: 0.968 ± 0.281
0.069HisCys: 0.069 ± 0.063
0.968HisAsp: 0.968 ± 0.287
1.175HisGlu: 1.175 ± 0.3
0.76HisPhe: 0.76 ± 0.172
1.175HisGly: 1.175 ± 0.263
0.346HisHis: 0.346 ± 0.184
1.452HisIle: 1.452 ± 0.398
1.383HisLys: 1.383 ± 0.276
1.59HisLeu: 1.59 ± 0.29
0.484HisMet: 0.484 ± 0.226
1.037HisAsn: 1.037 ± 0.283
0.83HisPro: 0.83 ± 0.198
0.553HisGln: 0.553 ± 0.178
0.76HisArg: 0.76 ± 0.206
1.244HisSer: 1.244 ± 0.226
1.106HisThr: 1.106 ± 0.277
0.83HisVal: 0.83 ± 0.282
0.346HisTrp: 0.346 ± 0.168
1.106HisTyr: 1.106 ± 0.236
0.0HisXaa: 0.0 ± 0.0
Ile
4.148IleAla: 4.148 ± 0.549
0.346IleCys: 0.346 ± 0.173
4.977IleAsp: 4.977 ± 0.664
5.53IleGlu: 5.53 ± 0.567
3.042IlePhe: 3.042 ± 0.579
3.387IleGly: 3.387 ± 0.552
1.797IleHis: 1.797 ± 0.373
5.046IleIle: 5.046 ± 0.847
7.881IleLys: 7.881 ± 0.736
5.254IleLeu: 5.254 ± 0.768
1.383IleMet: 1.383 ± 0.309
4.977IleAsn: 4.977 ± 0.531
2.419IlePro: 2.419 ± 0.381
1.866IleGln: 1.866 ± 0.288
3.526IleArg: 3.526 ± 0.504
5.046IleSer: 5.046 ± 0.573
4.079IleThr: 4.079 ± 0.504
3.871IleVal: 3.871 ± 0.694
0.484IleTrp: 0.484 ± 0.196
2.765IleTyr: 2.765 ± 0.547
0.0IleXaa: 0.0 ± 0.0
Lys
7.811LysAla: 7.811 ± 1.074
0.207LysCys: 0.207 ± 0.126
5.323LysAsp: 5.323 ± 0.539
8.503LysGlu: 8.503 ± 0.881
2.281LysPhe: 2.281 ± 0.343
5.599LysGly: 5.599 ± 0.977
1.797LysHis: 1.797 ± 0.422
6.152LysIle: 6.152 ± 0.732
7.881LysLys: 7.881 ± 0.921
8.987LysLeu: 8.987 ± 1.023
2.903LysMet: 2.903 ± 0.421
5.807LysAsn: 5.807 ± 0.7
2.212LysPro: 2.212 ± 0.375
5.254LysGln: 5.254 ± 0.595
3.664LysArg: 3.664 ± 0.695
6.221LysSer: 6.221 ± 1.287
5.461LysThr: 5.461 ± 0.566
5.185LysVal: 5.185 ± 0.597
1.797LysTrp: 1.797 ± 0.438
5.53LysTyr: 5.53 ± 0.771
0.0LysXaa: 0.0 ± 0.0
Leu
4.562LeuAla: 4.562 ± 0.775
0.553LeuCys: 0.553 ± 0.21
4.908LeuAsp: 4.908 ± 0.861
6.636LeuGlu: 6.636 ± 0.727
3.18LeuPhe: 3.18 ± 0.386
4.632LeuGly: 4.632 ± 0.883
1.244LeuHis: 1.244 ± 0.402
5.392LeuIle: 5.392 ± 0.668
9.056LeuLys: 9.056 ± 1.311
6.567LeuLeu: 6.567 ± 0.768
2.419LeuMet: 2.419 ± 0.418
5.599LeuAsn: 5.599 ± 0.622
2.834LeuPro: 2.834 ± 0.504
2.765LeuGln: 2.765 ± 0.565
3.526LeuArg: 3.526 ± 0.625
5.323LeuSer: 5.323 ± 0.644
5.738LeuThr: 5.738 ± 0.682
4.079LeuVal: 4.079 ± 0.426
0.484LeuTrp: 0.484 ± 0.203
3.111LeuTyr: 3.111 ± 0.733
0.0LeuXaa: 0.0 ± 0.0
Met
1.244MetAla: 1.244 ± 0.254
0.138MetCys: 0.138 ± 0.117
1.244MetAsp: 1.244 ± 0.32
1.659MetGlu: 1.659 ± 0.321
1.037MetPhe: 1.037 ± 0.3
1.521MetGly: 1.521 ± 0.504
0.346MetHis: 0.346 ± 0.174
1.59MetIle: 1.59 ± 0.219
2.696MetLys: 2.696 ± 0.502
1.936MetLeu: 1.936 ± 0.467
0.415MetMet: 0.415 ± 0.184
2.074MetAsn: 2.074 ± 0.451
1.106MetPro: 1.106 ± 0.213
1.866MetGln: 1.866 ± 0.402
1.037MetArg: 1.037 ± 0.261
2.143MetSer: 2.143 ± 0.428
2.074MetThr: 2.074 ± 0.289
1.175MetVal: 1.175 ± 0.229
0.346MetTrp: 0.346 ± 0.127
0.968MetTyr: 0.968 ± 0.229
0.0MetXaa: 0.0 ± 0.0
Asn
3.664AsnAla: 3.664 ± 0.543
0.207AsnCys: 0.207 ± 0.117
4.009AsnAsp: 4.009 ± 0.476
4.701AsnGlu: 4.701 ± 0.608
2.35AsnPhe: 2.35 ± 0.457
4.701AsnGly: 4.701 ± 0.554
0.968AsnHis: 0.968 ± 0.272
4.77AsnIle: 4.77 ± 0.639
6.636AsnLys: 6.636 ± 0.72
4.632AsnLeu: 4.632 ± 0.465
1.383AsnMet: 1.383 ± 0.242
3.664AsnAsn: 3.664 ± 0.59
2.627AsnPro: 2.627 ± 0.348
2.419AsnGln: 2.419 ± 0.422
2.834AsnArg: 2.834 ± 0.394
4.424AsnSer: 4.424 ± 0.653
3.664AsnThr: 3.664 ± 0.405
3.456AsnVal: 3.456 ± 0.519
1.106AsnTrp: 1.106 ± 0.343
2.903AsnTyr: 2.903 ± 0.584
0.0AsnXaa: 0.0 ± 0.0
Pro
1.037ProAla: 1.037 ± 0.205
0.277ProCys: 0.277 ± 0.136
1.452ProAsp: 1.452 ± 0.304
2.212ProGlu: 2.212 ± 0.409
1.313ProPhe: 1.313 ± 0.326
1.659ProGly: 1.659 ± 0.356
0.277ProHis: 0.277 ± 0.124
1.728ProIle: 1.728 ± 0.32
2.143ProLys: 2.143 ± 0.428
2.281ProLeu: 2.281 ± 0.45
0.691ProMet: 0.691 ± 0.251
2.143ProAsn: 2.143 ± 0.387
0.76ProPro: 0.76 ± 0.238
1.175ProGln: 1.175 ± 0.281
1.244ProArg: 1.244 ± 0.268
2.212ProSer: 2.212 ± 0.382
1.659ProThr: 1.659 ± 0.348
1.313ProVal: 1.313 ± 0.405
0.346ProTrp: 0.346 ± 0.141
1.106ProTyr: 1.106 ± 0.277
0.0ProXaa: 0.0 ± 0.0
Gln
2.419GlnAla: 2.419 ± 0.375
0.277GlnCys: 0.277 ± 0.143
2.005GlnAsp: 2.005 ± 0.362
2.765GlnGlu: 2.765 ± 0.507
1.659GlnPhe: 1.659 ± 0.291
2.143GlnGly: 2.143 ± 0.445
0.83GlnHis: 0.83 ± 0.262
2.765GlnIle: 2.765 ± 0.494
2.972GlnLys: 2.972 ± 0.409
3.249GlnLeu: 3.249 ± 0.481
1.175GlnMet: 1.175 ± 0.317
2.005GlnAsn: 2.005 ± 0.409
0.968GlnPro: 0.968 ± 0.218
1.106GlnGln: 1.106 ± 0.339
1.936GlnArg: 1.936 ± 0.446
2.35GlnSer: 2.35 ± 0.318
1.244GlnThr: 1.244 ± 0.389
2.212GlnVal: 2.212 ± 0.411
0.484GlnTrp: 0.484 ± 0.193
1.521GlnTyr: 1.521 ± 0.322
0.0GlnXaa: 0.0 ± 0.0
Arg
2.281ArgAla: 2.281 ± 0.535
0.138ArgCys: 0.138 ± 0.097
2.903ArgAsp: 2.903 ± 0.333
2.35ArgGlu: 2.35 ± 0.466
1.936ArgPhe: 1.936 ± 0.375
1.936ArgGly: 1.936 ± 0.383
0.76ArgHis: 0.76 ± 0.221
3.456ArgIle: 3.456 ± 0.435
4.079ArgLys: 4.079 ± 0.432
3.318ArgLeu: 3.318 ± 0.669
0.83ArgMet: 0.83 ± 0.233
2.765ArgAsn: 2.765 ± 0.454
0.691ArgPro: 0.691 ± 0.282
1.59ArgGln: 1.59 ± 0.319
1.866ArgArg: 1.866 ± 0.378
2.074ArgSer: 2.074 ± 0.355
2.558ArgThr: 2.558 ± 0.453
1.797ArgVal: 1.797 ± 0.333
0.346ArgTrp: 0.346 ± 0.143
2.005ArgTyr: 2.005 ± 0.399
0.0ArgXaa: 0.0 ± 0.0
Ser
3.871SerAla: 3.871 ± 0.755
0.277SerCys: 0.277 ± 0.145
4.77SerAsp: 4.77 ± 0.51
4.701SerGlu: 4.701 ± 0.579
2.834SerPhe: 2.834 ± 0.521
4.424SerGly: 4.424 ± 0.865
0.968SerHis: 0.968 ± 0.23
4.217SerIle: 4.217 ± 0.545
6.567SerLys: 6.567 ± 1.133
4.493SerLeu: 4.493 ± 0.422
2.212SerMet: 2.212 ± 0.376
4.839SerAsn: 4.839 ± 0.566
1.728SerPro: 1.728 ± 0.426
2.558SerGln: 2.558 ± 0.409
2.143SerArg: 2.143 ± 0.364
4.079SerSer: 4.079 ± 0.722
3.387SerThr: 3.387 ± 0.542
4.217SerVal: 4.217 ± 0.609
0.968SerTrp: 0.968 ± 0.24
2.281SerTyr: 2.281 ± 0.409
0.0SerXaa: 0.0 ± 0.0
Thr
3.318ThrAla: 3.318 ± 0.59
0.207ThrCys: 0.207 ± 0.119
3.249ThrAsp: 3.249 ± 0.533
4.009ThrGlu: 4.009 ± 0.515
2.558ThrPhe: 2.558 ± 0.329
3.94ThrGly: 3.94 ± 0.605
1.797ThrHis: 1.797 ± 0.396
4.562ThrIle: 4.562 ± 0.623
5.185ThrLys: 5.185 ± 0.746
4.148ThrLeu: 4.148 ± 0.436
0.968ThrMet: 0.968 ± 0.241
3.387ThrAsn: 3.387 ± 0.568
2.212ThrPro: 2.212 ± 0.287
1.728ThrGln: 1.728 ± 0.278
2.005ThrArg: 2.005 ± 0.33
3.526ThrSer: 3.526 ± 0.506
2.972ThrThr: 2.972 ± 0.495
4.079ThrVal: 4.079 ± 0.46
0.484ThrTrp: 0.484 ± 0.191
2.419ThrTyr: 2.419 ± 0.479
0.0ThrXaa: 0.0 ± 0.0
Val
2.972ValAla: 2.972 ± 0.515
0.207ValCys: 0.207 ± 0.118
3.94ValAsp: 3.94 ± 0.645
5.046ValGlu: 5.046 ± 0.577
2.558ValPhe: 2.558 ± 0.522
3.318ValGly: 3.318 ± 0.533
0.899ValHis: 0.899 ± 0.235
4.424ValIle: 4.424 ± 0.526
4.977ValLys: 4.977 ± 0.644
4.355ValLeu: 4.355 ± 0.505
1.452ValMet: 1.452 ± 0.283
3.871ValAsn: 3.871 ± 0.538
1.452ValPro: 1.452 ± 0.305
2.005ValGln: 2.005 ± 0.347
2.143ValArg: 2.143 ± 0.398
4.562ValSer: 4.562 ± 0.566
3.042ValThr: 3.042 ± 0.556
3.111ValVal: 3.111 ± 0.44
0.415ValTrp: 0.415 ± 0.179
1.936ValTyr: 1.936 ± 0.452
0.0ValXaa: 0.0 ± 0.0
Trp
0.622TrpAla: 0.622 ± 0.19
0.0TrpCys: 0.0 ± 0.0
0.415TrpAsp: 0.415 ± 0.22
0.83TrpGlu: 0.83 ± 0.285
1.175TrpPhe: 1.175 ± 0.338
0.691TrpGly: 0.691 ± 0.256
0.0TrpHis: 0.0 ± 0.0
0.968TrpIle: 0.968 ± 0.274
0.76TrpLys: 0.76 ± 0.242
1.244TrpLeu: 1.244 ± 0.278
0.346TrpMet: 0.346 ± 0.197
0.899TrpAsn: 0.899 ± 0.222
0.346TrpPro: 0.346 ± 0.233
0.553TrpGln: 0.553 ± 0.173
0.415TrpArg: 0.415 ± 0.174
1.175TrpSer: 1.175 ± 0.403
0.622TrpThr: 0.622 ± 0.199
0.83TrpVal: 0.83 ± 0.224
0.138TrpTrp: 0.138 ± 0.122
0.553TrpTyr: 0.553 ± 0.195
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.35TyrAla: 2.35 ± 0.279
0.415TyrCys: 0.415 ± 0.155
2.765TyrAsp: 2.765 ± 0.637
2.972TyrGlu: 2.972 ± 0.554
1.383TyrPhe: 1.383 ± 0.363
2.696TyrGly: 2.696 ± 0.567
1.175TyrHis: 1.175 ± 0.312
2.627TyrIle: 2.627 ± 0.466
3.802TyrLys: 3.802 ± 0.416
3.664TyrLeu: 3.664 ± 0.532
1.659TyrMet: 1.659 ± 0.279
2.696TyrAsn: 2.696 ± 0.57
1.037TyrPro: 1.037 ± 0.284
1.728TyrGln: 1.728 ± 0.237
1.728TyrArg: 1.728 ± 0.45
3.18TyrSer: 3.18 ± 0.39
2.35TyrThr: 2.35 ± 0.45
2.765TyrVal: 2.765 ± 0.479
0.622TyrTrp: 0.622 ± 0.177
1.521TyrTyr: 1.521 ± 0.385
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 65 proteins (14467 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski