Amino acid dipepetide frequency for Escherichia phage vB_EcoS_IME347

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.265AlaAla: 8.265 ± 0.967
1.124AlaCys: 1.124 ± 0.319
4.298AlaAsp: 4.298 ± 0.549
6.149AlaGlu: 6.149 ± 0.786
2.711AlaPhe: 2.711 ± 0.422
7.273AlaGly: 7.273 ± 0.696
1.058AlaHis: 1.058 ± 0.266
6.348AlaIle: 6.348 ± 0.703
5.62AlaLys: 5.62 ± 0.805
6.149AlaLeu: 6.149 ± 0.701
2.711AlaMet: 2.711 ± 0.386
4.166AlaAsn: 4.166 ± 0.83
2.116AlaPro: 2.116 ± 0.419
4.033AlaGln: 4.033 ± 0.705
4.827AlaArg: 4.827 ± 0.72
5.223AlaSer: 5.223 ± 0.589
4.298AlaThr: 4.298 ± 0.705
5.29AlaVal: 5.29 ± 0.632
1.19AlaTrp: 1.19 ± 0.313
2.843AlaTyr: 2.843 ± 0.448
0.0AlaXaa: 0.0 ± 0.0
Cys
1.124CysAla: 1.124 ± 0.315
0.331CysCys: 0.331 ± 0.152
0.727CysAsp: 0.727 ± 0.2
1.058CysGlu: 1.058 ± 0.31
0.264CysPhe: 0.264 ± 0.124
1.653CysGly: 1.653 ± 0.406
0.463CysHis: 0.463 ± 0.192
0.661CysIle: 0.661 ± 0.219
0.661CysLys: 0.661 ± 0.243
0.86CysLeu: 0.86 ± 0.23
0.331CysMet: 0.331 ± 0.123
0.397CysAsn: 0.397 ± 0.18
0.463CysPro: 0.463 ± 0.207
0.132CysGln: 0.132 ± 0.097
1.058CysArg: 1.058 ± 0.276
0.793CysSer: 0.793 ± 0.218
0.727CysThr: 0.727 ± 0.241
1.058CysVal: 1.058 ± 0.259
0.264CysTrp: 0.264 ± 0.128
0.595CysTyr: 0.595 ± 0.195
0.0CysXaa: 0.0 ± 0.0
Asp
5.223AspAla: 5.223 ± 0.516
0.595AspCys: 0.595 ± 0.194
3.769AspAsp: 3.769 ± 0.582
3.703AspGlu: 3.703 ± 0.431
3.042AspPhe: 3.042 ± 0.416
6.281AspGly: 6.281 ± 0.713
0.595AspHis: 0.595 ± 0.181
2.579AspIle: 2.579 ± 0.35
3.504AspLys: 3.504 ± 0.564
4.364AspLeu: 4.364 ± 0.615
1.653AspMet: 1.653 ± 0.335
3.042AspAsn: 3.042 ± 0.471
2.05AspPro: 2.05 ± 0.339
1.653AspGln: 1.653 ± 0.299
2.711AspArg: 2.711 ± 0.454
4.099AspSer: 4.099 ± 0.406
2.446AspThr: 2.446 ± 0.355
5.223AspVal: 5.223 ± 0.532
0.727AspTrp: 0.727 ± 0.2
2.777AspTyr: 2.777 ± 0.442
0.0AspXaa: 0.0 ± 0.0
Glu
6.48GluAla: 6.48 ± 0.688
0.463GluCys: 0.463 ± 0.206
3.769GluAsp: 3.769 ± 0.501
4.364GluGlu: 4.364 ± 0.718
3.835GluPhe: 3.835 ± 0.49
4.562GluGly: 4.562 ± 0.531
1.058GluHis: 1.058 ± 0.227
5.29GluIle: 5.29 ± 0.636
4.628GluLys: 4.628 ± 0.653
5.157GluLeu: 5.157 ± 0.629
2.711GluMet: 2.711 ± 0.436
2.645GluAsn: 2.645 ± 0.382
2.579GluPro: 2.579 ± 0.475
3.174GluGln: 3.174 ± 0.524
3.306GluArg: 3.306 ± 0.511
4.099GluSer: 4.099 ± 0.489
3.901GluThr: 3.901 ± 0.434
5.157GluVal: 5.157 ± 0.685
0.727GluTrp: 0.727 ± 0.261
2.579GluTyr: 2.579 ± 0.327
0.0GluXaa: 0.0 ± 0.0
Phe
3.372PheAla: 3.372 ± 0.394
0.529PheCys: 0.529 ± 0.233
3.637PheAsp: 3.637 ± 0.525
2.843PheGlu: 2.843 ± 0.493
1.389PhePhe: 1.389 ± 0.338
3.306PheGly: 3.306 ± 0.574
0.793PheHis: 0.793 ± 0.21
2.711PheIle: 2.711 ± 0.42
3.57PheLys: 3.57 ± 0.509
2.182PheLeu: 2.182 ± 0.319
1.389PheMet: 1.389 ± 0.326
2.38PheAsn: 2.38 ± 0.32
1.124PhePro: 1.124 ± 0.296
1.322PheGln: 1.322 ± 0.31
1.984PheArg: 1.984 ± 0.311
2.314PheSer: 2.314 ± 0.458
2.711PheThr: 2.711 ± 0.469
2.38PheVal: 2.38 ± 0.361
0.86PheTrp: 0.86 ± 0.22
1.521PheTyr: 1.521 ± 0.336
0.0PheXaa: 0.0 ± 0.0
Gly
4.761GlyAla: 4.761 ± 0.777
1.322GlyCys: 1.322 ± 0.314
4.364GlyAsp: 4.364 ± 0.598
6.017GlyGlu: 6.017 ± 0.608
3.438GlyPhe: 3.438 ± 0.47
6.414GlyGly: 6.414 ± 0.791
1.256GlyHis: 1.256 ± 0.322
4.232GlyIle: 4.232 ± 0.515
7.538GlyLys: 7.538 ± 0.628
4.695GlyLeu: 4.695 ± 0.474
2.909GlyMet: 2.909 ± 0.536
4.364GlyAsn: 4.364 ± 0.678
0.066GlyPro: 0.066 ± 0.069
2.777GlyGln: 2.777 ± 0.466
3.637GlyArg: 3.637 ± 0.442
4.628GlySer: 4.628 ± 0.52
3.438GlyThr: 3.438 ± 0.428
6.083GlyVal: 6.083 ± 0.616
1.521GlyTrp: 1.521 ± 0.271
3.042GlyTyr: 3.042 ± 0.534
0.0GlyXaa: 0.0 ± 0.0
His
1.322HisAla: 1.322 ± 0.359
0.132HisCys: 0.132 ± 0.095
1.19HisAsp: 1.19 ± 0.34
1.256HisGlu: 1.256 ± 0.292
1.256HisPhe: 1.256 ± 0.293
1.521HisGly: 1.521 ± 0.307
0.595HisHis: 0.595 ± 0.225
0.992HisIle: 0.992 ± 0.24
1.389HisLys: 1.389 ± 0.335
1.389HisLeu: 1.389 ± 0.329
0.264HisMet: 0.264 ± 0.125
0.793HisAsn: 0.793 ± 0.296
0.793HisPro: 0.793 ± 0.229
0.397HisGln: 0.397 ± 0.148
0.727HisArg: 0.727 ± 0.239
0.661HisSer: 0.661 ± 0.216
1.124HisThr: 1.124 ± 0.261
1.124HisVal: 1.124 ± 0.244
0.331HisTrp: 0.331 ± 0.141
1.058HisTyr: 1.058 ± 0.27
0.0HisXaa: 0.0 ± 0.0
Ile
6.215IleAla: 6.215 ± 0.684
0.529IleCys: 0.529 ± 0.148
4.628IleAsp: 4.628 ± 0.475
4.959IleGlu: 4.959 ± 0.46
1.917IlePhe: 1.917 ± 0.314
3.637IleGly: 3.637 ± 0.482
0.727IleHis: 0.727 ± 0.219
3.372IleIle: 3.372 ± 0.549
4.43IleLys: 4.43 ± 0.582
3.769IleLeu: 3.769 ± 0.595
1.521IleMet: 1.521 ± 0.339
3.637IleAsn: 3.637 ± 0.627
2.446IlePro: 2.446 ± 0.389
2.182IleGln: 2.182 ± 0.435
3.438IleArg: 3.438 ± 0.531
3.504IleSer: 3.504 ± 0.402
4.232IleThr: 4.232 ± 0.583
4.099IleVal: 4.099 ± 0.515
0.661IleTrp: 0.661 ± 0.209
1.851IleTyr: 1.851 ± 0.355
0.0IleXaa: 0.0 ± 0.0
Lys
7.141LysAla: 7.141 ± 0.787
1.058LysCys: 1.058 ± 0.285
3.637LysAsp: 3.637 ± 0.502
4.827LysGlu: 4.827 ± 0.685
2.909LysPhe: 2.909 ± 0.414
3.901LysGly: 3.901 ± 0.498
1.917LysHis: 1.917 ± 0.364
3.57LysIle: 3.57 ± 0.538
4.364LysLys: 4.364 ± 0.51
4.893LysLeu: 4.893 ± 0.391
2.909LysMet: 2.909 ± 0.406
2.909LysAsn: 2.909 ± 0.385
3.108LysPro: 3.108 ± 0.517
1.851LysGln: 1.851 ± 0.325
3.901LysArg: 3.901 ± 0.56
3.967LysSer: 3.967 ± 0.411
3.042LysThr: 3.042 ± 0.462
5.488LysVal: 5.488 ± 0.631
0.992LysTrp: 0.992 ± 0.285
2.645LysTyr: 2.645 ± 0.343
0.0LysXaa: 0.0 ± 0.0
Leu
5.223LeuAla: 5.223 ± 0.696
0.595LeuCys: 0.595 ± 0.169
3.967LeuAsp: 3.967 ± 0.407
4.232LeuGlu: 4.232 ± 0.568
1.917LeuPhe: 1.917 ± 0.277
3.901LeuGly: 3.901 ± 0.516
1.389LeuHis: 1.389 ± 0.372
4.232LeuIle: 4.232 ± 0.428
5.223LeuLys: 5.223 ± 0.715
3.438LeuLeu: 3.438 ± 0.378
1.851LeuMet: 1.851 ± 0.422
3.769LeuAsn: 3.769 ± 0.59
3.372LeuPro: 3.372 ± 0.487
2.38LeuGln: 2.38 ± 0.431
3.703LeuArg: 3.703 ± 0.488
4.695LeuSer: 4.695 ± 0.648
4.43LeuThr: 4.43 ± 0.613
4.033LeuVal: 4.033 ± 0.508
0.926LeuTrp: 0.926 ± 0.196
2.116LeuTyr: 2.116 ± 0.37
0.0LeuXaa: 0.0 ± 0.0
Met
2.116MetAla: 2.116 ± 0.377
0.264MetCys: 0.264 ± 0.118
1.455MetAsp: 1.455 ± 0.324
1.984MetGlu: 1.984 ± 0.35
1.058MetPhe: 1.058 ± 0.251
1.521MetGly: 1.521 ± 0.362
0.595MetHis: 0.595 ± 0.196
2.843MetIle: 2.843 ± 0.446
3.24MetLys: 3.24 ± 0.434
2.645MetLeu: 2.645 ± 0.449
1.389MetMet: 1.389 ± 0.304
1.19MetAsn: 1.19 ± 0.294
0.926MetPro: 0.926 ± 0.23
1.322MetGln: 1.322 ± 0.262
1.785MetArg: 1.785 ± 0.364
2.116MetSer: 2.116 ± 0.329
1.785MetThr: 1.785 ± 0.345
1.917MetVal: 1.917 ± 0.307
0.264MetTrp: 0.264 ± 0.111
0.992MetTyr: 0.992 ± 0.255
0.0MetXaa: 0.0 ± 0.0
Asn
4.099AsnAla: 4.099 ± 0.538
0.529AsnCys: 0.529 ± 0.192
3.174AsnAsp: 3.174 ± 0.438
3.57AsnGlu: 3.57 ± 0.448
1.719AsnPhe: 1.719 ± 0.3
5.554AsnGly: 5.554 ± 0.625
1.19AsnHis: 1.19 ± 0.259
1.984AsnIle: 1.984 ± 0.316
3.438AsnLys: 3.438 ± 0.445
3.372AsnLeu: 3.372 ± 0.55
0.661AsnMet: 0.661 ± 0.233
2.314AsnAsn: 2.314 ± 0.553
1.851AsnPro: 1.851 ± 0.362
2.314AsnGln: 2.314 ± 0.528
1.653AsnArg: 1.653 ± 0.353
2.579AsnSer: 2.579 ± 0.433
2.314AsnThr: 2.314 ± 0.45
3.372AsnVal: 3.372 ± 0.568
0.727AsnTrp: 0.727 ± 0.184
1.587AsnTyr: 1.587 ± 0.273
0.0AsnXaa: 0.0 ± 0.0
Pro
2.38ProAla: 2.38 ± 0.325
0.727ProCys: 0.727 ± 0.247
2.314ProAsp: 2.314 ± 0.454
2.513ProGlu: 2.513 ± 0.531
1.653ProPhe: 1.653 ± 0.28
2.909ProGly: 2.909 ± 0.425
0.793ProHis: 0.793 ± 0.228
2.05ProIle: 2.05 ± 0.387
1.587ProLys: 1.587 ± 0.392
1.587ProLeu: 1.587 ± 0.253
1.322ProMet: 1.322 ± 0.291
1.785ProAsn: 1.785 ± 0.409
0.992ProPro: 0.992 ± 0.279
1.19ProGln: 1.19 ± 0.38
1.455ProArg: 1.455 ± 0.296
1.587ProSer: 1.587 ± 0.345
1.785ProThr: 1.785 ± 0.332
3.306ProVal: 3.306 ± 0.417
0.397ProTrp: 0.397 ± 0.168
1.124ProTyr: 1.124 ± 0.253
0.0ProXaa: 0.0 ± 0.0
Gln
3.372GlnAla: 3.372 ± 0.506
0.463GlnCys: 0.463 ± 0.158
2.05GlnAsp: 2.05 ± 0.398
2.314GlnGlu: 2.314 ± 0.357
1.521GlnPhe: 1.521 ± 0.295
2.248GlnGly: 2.248 ± 0.459
0.264GlnHis: 0.264 ± 0.112
2.711GlnIle: 2.711 ± 0.337
2.777GlnLys: 2.777 ± 0.636
3.108GlnLeu: 3.108 ± 0.549
0.926GlnMet: 0.926 ± 0.25
1.19GlnAsn: 1.19 ± 0.248
0.992GlnPro: 0.992 ± 0.293
2.513GlnGln: 2.513 ± 0.806
2.182GlnArg: 2.182 ± 0.379
2.843GlnSer: 2.843 ± 0.451
1.521GlnThr: 1.521 ± 0.312
3.438GlnVal: 3.438 ± 0.408
0.529GlnTrp: 0.529 ± 0.175
1.653GlnTyr: 1.653 ± 0.35
0.0GlnXaa: 0.0 ± 0.0
Arg
4.298ArgAla: 4.298 ± 0.491
0.992ArgCys: 0.992 ± 0.322
2.182ArgAsp: 2.182 ± 0.325
4.099ArgGlu: 4.099 ± 0.511
2.843ArgPhe: 2.843 ± 0.436
2.975ArgGly: 2.975 ± 0.395
0.926ArgHis: 0.926 ± 0.304
3.306ArgIle: 3.306 ± 0.493
3.769ArgLys: 3.769 ± 0.594
3.438ArgLeu: 3.438 ± 0.381
1.719ArgMet: 1.719 ± 0.318
2.579ArgAsn: 2.579 ± 0.397
1.455ArgPro: 1.455 ± 0.293
2.116ArgGln: 2.116 ± 0.397
2.711ArgArg: 2.711 ± 0.443
2.182ArgSer: 2.182 ± 0.456
1.851ArgThr: 1.851 ± 0.307
4.496ArgVal: 4.496 ± 0.736
0.926ArgTrp: 0.926 ± 0.273
1.984ArgTyr: 1.984 ± 0.376
0.0ArgXaa: 0.0 ± 0.0
Ser
4.959SerAla: 4.959 ± 0.563
0.661SerCys: 0.661 ± 0.2
3.372SerAsp: 3.372 ± 0.506
4.827SerGlu: 4.827 ± 0.511
3.306SerPhe: 3.306 ± 0.448
6.215SerGly: 6.215 ± 0.598
0.926SerHis: 0.926 ± 0.267
3.504SerIle: 3.504 ± 0.644
3.306SerLys: 3.306 ± 0.443
4.298SerLeu: 4.298 ± 0.466
1.917SerMet: 1.917 ± 0.389
2.05SerAsn: 2.05 ± 0.39
2.182SerPro: 2.182 ± 0.329
2.579SerGln: 2.579 ± 0.369
2.975SerArg: 2.975 ± 0.387
2.248SerSer: 2.248 ± 0.301
3.372SerThr: 3.372 ± 0.42
4.232SerVal: 4.232 ± 0.608
0.992SerTrp: 0.992 ± 0.212
1.521SerTyr: 1.521 ± 0.383
0.0SerXaa: 0.0 ± 0.0
Thr
4.893ThrAla: 4.893 ± 0.648
0.86ThrCys: 0.86 ± 0.238
3.042ThrAsp: 3.042 ± 0.536
3.306ThrGlu: 3.306 ± 0.464
2.513ThrPhe: 2.513 ± 0.362
5.091ThrGly: 5.091 ± 0.463
1.124ThrHis: 1.124 ± 0.273
3.372ThrIle: 3.372 ± 0.449
2.843ThrLys: 2.843 ± 0.612
2.909ThrLeu: 2.909 ± 0.34
1.322ThrMet: 1.322 ± 0.265
2.645ThrAsn: 2.645 ± 0.432
2.711ThrPro: 2.711 ± 0.434
1.785ThrGln: 1.785 ± 0.365
2.05ThrArg: 2.05 ± 0.341
3.703ThrSer: 3.703 ± 0.476
2.248ThrThr: 2.248 ± 0.323
3.504ThrVal: 3.504 ± 0.469
0.86ThrTrp: 0.86 ± 0.218
2.182ThrTyr: 2.182 ± 0.359
0.0ThrXaa: 0.0 ± 0.0
Val
5.752ValAla: 5.752 ± 0.597
1.653ValCys: 1.653 ± 0.36
4.628ValAsp: 4.628 ± 0.57
4.893ValGlu: 4.893 ± 0.579
3.108ValPhe: 3.108 ± 0.474
3.769ValGly: 3.769 ± 0.481
1.653ValHis: 1.653 ± 0.321
5.223ValIle: 5.223 ± 0.712
4.827ValLys: 4.827 ± 0.572
3.967ValLeu: 3.967 ± 0.546
2.579ValMet: 2.579 ± 0.386
3.24ValAsn: 3.24 ± 0.443
2.182ValPro: 2.182 ± 0.383
2.579ValGln: 2.579 ± 0.418
3.504ValArg: 3.504 ± 0.544
5.356ValSer: 5.356 ± 0.647
4.959ValThr: 4.959 ± 0.549
4.232ValVal: 4.232 ± 0.562
1.455ValTrp: 1.455 ± 0.302
1.851ValTyr: 1.851 ± 0.333
0.0ValXaa: 0.0 ± 0.0
Trp
1.19TrpAla: 1.19 ± 0.231
0.198TrpCys: 0.198 ± 0.125
1.124TrpAsp: 1.124 ± 0.258
0.595TrpGlu: 0.595 ± 0.19
0.397TrpPhe: 0.397 ± 0.21
1.058TrpGly: 1.058 ± 0.209
0.397TrpHis: 0.397 ± 0.176
0.992TrpIle: 0.992 ± 0.272
1.058TrpLys: 1.058 ± 0.256
1.19TrpLeu: 1.19 ± 0.244
0.463TrpMet: 0.463 ± 0.161
0.926TrpAsn: 0.926 ± 0.217
0.331TrpPro: 0.331 ± 0.167
0.529TrpGln: 0.529 ± 0.185
0.926TrpArg: 0.926 ± 0.239
1.058TrpSer: 1.058 ± 0.242
0.992TrpThr: 0.992 ± 0.238
0.926TrpVal: 0.926 ± 0.223
0.198TrpTrp: 0.198 ± 0.116
0.529TrpTyr: 0.529 ± 0.172
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.57TyrAla: 3.57 ± 0.437
0.595TyrCys: 0.595 ± 0.18
2.711TyrAsp: 2.711 ± 0.373
2.975TyrGlu: 2.975 ± 0.42
1.521TyrPhe: 1.521 ± 0.326
2.711TyrGly: 2.711 ± 0.331
0.595TyrHis: 0.595 ± 0.204
1.785TyrIle: 1.785 ± 0.279
1.256TyrLys: 1.256 ± 0.337
1.984TyrLeu: 1.984 ± 0.319
0.793TyrMet: 0.793 ± 0.25
1.984TyrAsn: 1.984 ± 0.378
1.719TyrPro: 1.719 ± 0.388
1.785TyrGln: 1.785 ± 0.308
2.314TyrArg: 2.314 ± 0.394
1.785TyrSer: 1.785 ± 0.38
1.785TyrThr: 1.785 ± 0.322
2.05TyrVal: 2.05 ± 0.301
0.529TyrTrp: 0.529 ± 0.224
1.19TyrTyr: 1.19 ± 0.302
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 87 proteins (15125 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski