Pectobacterium phage vB_PatP_CB1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Cbunavirus; Pectobacterium virus CB1

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P0PAQ7|A0A2P0PAQ7_9CAUD Uncharacterized protein OS=Pectobacterium phage vB_PatP_CB1 OX=1958917 GN=CB1_56 PE=4 SV=1
MM1 pKa = 7.38GGDD4 pKa = 4.71GEE6 pKa = 5.44CHH8 pKa = 6.96DD9 pKa = 5.41CPCGWTQPLNDD20 pKa = 4.61PEE22 pKa = 4.96EE23 pKa = 4.56VPQPMDD29 pKa = 3.24EE30 pKa = 4.18DD31 pKa = 4.08QIVSLRR37 pKa = 11.84AFLGEE42 pKa = 4.74HH43 pKa = 5.22WALWEE48 pKa = 4.02EE49 pKa = 4.34HH50 pKa = 6.44CEE52 pKa = 4.78QRR54 pKa = 11.84GEE56 pKa = 4.28DD57 pKa = 3.59PNEE60 pKa = 3.88IYY62 pKa = 11.12VEE64 pKa = 4.14VLGGEE69 pKa = 4.38DD70 pKa = 3.06

Molecular weight: 7.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P0PAX6|A0A2P0PAX6_9CAUD Uncharacterized protein OS=Pectobacterium phage vB_PatP_CB1 OX=1958917 GN=CB1_70 PE=4 SV=1
MM1 pKa = 7.7SKK3 pKa = 10.73PEE5 pKa = 4.72LIIMGIGVLAFLAGLFLAWLQRR27 pKa = 11.84RR28 pKa = 11.84CHH30 pKa = 6.27HH31 pKa = 5.84KK32 pKa = 10.25HH33 pKa = 4.96FLVRR37 pKa = 11.84TGQGGIRR44 pKa = 11.84ILSCSKK50 pKa = 10.28CGKK53 pKa = 9.62IKK55 pKa = 11.17YY56 pKa = 7.55EE57 pKa = 3.95

Molecular weight: 6.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

23567

33

3693

243.0

27.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.233 ± 0.652

0.84 ± 0.151

5.983 ± 0.145

6.165 ± 0.263

3.573 ± 0.187

6.81 ± 0.214

1.96 ± 0.156

5.38 ± 0.277

6.064 ± 0.394

8.724 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.868 ± 0.135

5.262 ± 0.261

4.303 ± 0.219

4.71 ± 0.386

4.952 ± 0.248

5.567 ± 0.222

6.454 ± 0.365

6.458 ± 0.24

1.277 ± 0.142

3.416 ± 0.27
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski