Amino acid dipepetide frequency for Paenibacillus faecis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.46AlaAla: 9.46 ± 0.111
0.734AlaCys: 0.734 ± 0.025
4.454AlaAsp: 4.454 ± 0.063
6.644AlaGlu: 6.644 ± 0.083
3.346AlaPhe: 3.346 ± 0.041
7.631AlaGly: 7.631 ± 0.084
1.35AlaHis: 1.35 ± 0.026
4.818AlaIle: 4.818 ± 0.057
4.42AlaLys: 4.42 ± 0.06
8.66AlaLeu: 8.66 ± 0.083
2.313AlaMet: 2.313 ± 0.036
2.482AlaAsn: 2.482 ± 0.04
2.889AlaPro: 2.889 ± 0.048
2.59AlaGln: 2.59 ± 0.036
3.967AlaArg: 3.967 ± 0.051
4.962AlaSer: 4.962 ± 0.063
3.355AlaThr: 3.355 ± 0.049
6.839AlaVal: 6.839 ± 0.077
1.052AlaTrp: 1.052 ± 0.026
2.834AlaTyr: 2.834 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
0.537CysAla: 0.537 ± 0.019
0.103CysCys: 0.103 ± 0.009
0.375CysAsp: 0.375 ± 0.016
0.437CysGlu: 0.437 ± 0.017
0.323CysPhe: 0.323 ± 0.014
0.825CysGly: 0.825 ± 0.023
0.176CysHis: 0.176 ± 0.01
0.437CysIle: 0.437 ± 0.016
0.296CysLys: 0.296 ± 0.015
0.803CysLeu: 0.803 ± 0.023
0.173CysMet: 0.173 ± 0.01
0.249CysAsn: 0.249 ± 0.011
0.353CysPro: 0.353 ± 0.016
0.228CysGln: 0.228 ± 0.011
0.519CysArg: 0.519 ± 0.018
0.523CysSer: 0.523 ± 0.02
0.358CysThr: 0.358 ± 0.016
0.44CysVal: 0.44 ± 0.017
0.092CysTrp: 0.092 ± 0.008
0.255CysTyr: 0.255 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
3.863AspAla: 3.863 ± 0.055
0.374AspCys: 0.374 ± 0.014
2.313AspAsp: 2.313 ± 0.042
3.86AspGlu: 3.86 ± 0.053
2.289AspPhe: 2.289 ± 0.037
4.215AspGly: 4.215 ± 0.066
1.101AspHis: 1.101 ± 0.027
3.387AspIle: 3.387 ± 0.044
2.564AspLys: 2.564 ± 0.046
5.251AspLeu: 5.251 ± 0.055
1.255AspMet: 1.255 ± 0.026
1.633AspAsn: 1.633 ± 0.033
2.602AspPro: 2.602 ± 0.036
1.797AspGln: 1.797 ± 0.035
3.206AspArg: 3.206 ± 0.049
2.57AspSer: 2.57 ± 0.044
2.454AspThr: 2.454 ± 0.044
3.233AspVal: 3.233 ± 0.048
0.852AspTrp: 0.852 ± 0.026
2.14AspTyr: 2.14 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
6.785GluAla: 6.785 ± 0.081
0.38GluCys: 0.38 ± 0.015
3.351GluAsp: 3.351 ± 0.047
5.891GluGlu: 5.891 ± 0.072
2.321GluPhe: 2.321 ± 0.033
4.837GluGly: 4.837 ± 0.061
1.536GluHis: 1.536 ± 0.029
4.621GluIle: 4.621 ± 0.051
4.109GluLys: 4.109 ± 0.055
7.488GluLeu: 7.488 ± 0.088
2.113GluMet: 2.113 ± 0.035
2.541GluAsn: 2.541 ± 0.042
2.597GluPro: 2.597 ± 0.042
3.445GluGln: 3.445 ± 0.064
4.669GluArg: 4.669 ± 0.063
3.557GluSer: 3.557 ± 0.053
3.603GluThr: 3.603 ± 0.049
4.703GluVal: 4.703 ± 0.056
0.934GluTrp: 0.934 ± 0.024
2.13GluTyr: 2.13 ± 0.04
0.0GluXaa: 0.0 ± 0.0
Phe
3.403PheAla: 3.403 ± 0.053
0.372PheCys: 0.372 ± 0.015
2.205PheAsp: 2.205 ± 0.036
2.513PheGlu: 2.513 ± 0.042
1.869PhePhe: 1.869 ± 0.044
3.346PheGly: 3.346 ± 0.049
0.885PheHis: 0.885 ± 0.022
2.713PheIle: 2.713 ± 0.046
1.982PheLys: 1.982 ± 0.036
3.994PheLeu: 3.994 ± 0.058
1.15PheMet: 1.15 ± 0.028
1.493PheAsn: 1.493 ± 0.031
1.643PhePro: 1.643 ± 0.029
1.392PheGln: 1.392 ± 0.027
2.297PheArg: 2.297 ± 0.035
2.801PheSer: 2.801 ± 0.048
2.404PheThr: 2.404 ± 0.035
2.952PheVal: 2.952 ± 0.045
0.569PheTrp: 0.569 ± 0.019
1.512PheTyr: 1.512 ± 0.03
0.0PheXaa: 0.0 ± 0.0
Gly
5.973GlyAla: 5.973 ± 0.061
0.706GlyCys: 0.706 ± 0.023
3.807GlyAsp: 3.807 ± 0.057
5.359GlyGlu: 5.359 ± 0.062
3.392GlyPhe: 3.392 ± 0.043
6.445GlyGly: 6.445 ± 0.09
1.484GlyHis: 1.484 ± 0.034
5.467GlyIle: 5.467 ± 0.064
4.761GlyLys: 4.761 ± 0.057
7.663GlyLeu: 7.663 ± 0.072
2.405GlyMet: 2.405 ± 0.038
2.793GlyAsn: 2.793 ± 0.051
2.283GlyPro: 2.283 ± 0.04
2.714GlyGln: 2.714 ± 0.044
4.137GlyArg: 4.137 ± 0.052
4.806GlySer: 4.806 ± 0.062
4.595GlyThr: 4.595 ± 0.067
5.414GlyVal: 5.414 ± 0.06
1.096GlyTrp: 1.096 ± 0.028
3.065GlyTyr: 3.065 ± 0.046
0.0GlyXaa: 0.0 ± 0.0
His
1.516HisAla: 1.516 ± 0.03
0.191HisCys: 0.191 ± 0.01
0.934HisAsp: 0.934 ± 0.021
1.255HisGlu: 1.255 ± 0.026
1.032HisPhe: 1.032 ± 0.025
1.541HisGly: 1.541 ± 0.029
0.577HisHis: 0.577 ± 0.026
1.301HisIle: 1.301 ± 0.03
0.789HisLys: 0.789 ± 0.021
2.171HisLeu: 2.171 ± 0.076
0.506HisMet: 0.506 ± 0.017
0.59HisAsn: 0.59 ± 0.018
1.273HisPro: 1.273 ± 0.028
0.7HisGln: 0.7 ± 0.019
1.21HisArg: 1.21 ± 0.028
1.03HisSer: 1.03 ± 0.025
0.984HisThr: 0.984 ± 0.023
1.291HisVal: 1.291 ± 0.03
0.311HisTrp: 0.311 ± 0.012
0.809HisTyr: 0.809 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
5.629IleAla: 5.629 ± 0.064
0.581IleCys: 0.581 ± 0.018
3.338IleAsp: 3.338 ± 0.045
3.952IleGlu: 3.952 ± 0.051
2.33IlePhe: 2.33 ± 0.048
5.182IleGly: 5.182 ± 0.067
1.381IleHis: 1.381 ± 0.028
3.463IleIle: 3.463 ± 0.061
2.785IleLys: 2.785 ± 0.042
5.858IleLeu: 5.858 ± 0.072
1.463IleMet: 1.463 ± 0.034
2.109IleAsn: 2.109 ± 0.038
3.131IlePro: 3.131 ± 0.047
2.271IleGln: 2.271 ± 0.038
3.953IleArg: 3.953 ± 0.049
4.196IleSer: 4.196 ± 0.058
3.521IleThr: 3.521 ± 0.047
4.727IleVal: 4.727 ± 0.067
0.685IleTrp: 0.685 ± 0.02
2.103IleTyr: 2.103 ± 0.034
0.0IleXaa: 0.0 ± 0.0
Lys
4.349LysAla: 4.349 ± 0.056
0.252LysCys: 0.252 ± 0.013
2.778LysAsp: 2.778 ± 0.047
4.462LysGlu: 4.462 ± 0.054
1.623LysPhe: 1.623 ± 0.034
3.624LysGly: 3.624 ± 0.048
1.01LysHis: 1.01 ± 0.024
3.116LysIle: 3.116 ± 0.047
3.323LysLys: 3.323 ± 0.05
5.293LysLeu: 5.293 ± 0.057
1.55LysMet: 1.55 ± 0.028
2.04LysAsn: 2.04 ± 0.036
2.333LysPro: 2.333 ± 0.04
2.268LysGln: 2.268 ± 0.034
3.056LysArg: 3.056 ± 0.04
2.94LysSer: 2.94 ± 0.038
2.929LysThr: 2.929 ± 0.043
3.615LysVal: 3.615 ± 0.053
0.705LysTrp: 0.705 ± 0.022
1.85LysTyr: 1.85 ± 0.035
0.0LysXaa: 0.0 ± 0.0
Leu
8.755LeuAla: 8.755 ± 0.095
0.824LeuCys: 0.824 ± 0.023
5.338LeuAsp: 5.338 ± 0.056
6.891LeuGlu: 6.891 ± 0.077
4.609LeuPhe: 4.609 ± 0.067
7.185LeuGly: 7.185 ± 0.063
2.022LeuHis: 2.022 ± 0.032
6.377LeuIle: 6.377 ± 0.073
5.528LeuLys: 5.528 ± 0.06
11.329LeuLeu: 11.329 ± 0.116
2.607LeuMet: 2.607 ± 0.041
3.77LeuAsn: 3.77 ± 0.049
4.782LeuPro: 4.782 ± 0.055
3.807LeuGln: 3.807 ± 0.059
5.653LeuArg: 5.653 ± 0.065
6.915LeuSer: 6.915 ± 0.068
5.713LeuThr: 5.713 ± 0.071
6.445LeuVal: 6.445 ± 0.073
1.051LeuTrp: 1.051 ± 0.026
3.196LeuTyr: 3.196 ± 0.048
0.0LeuXaa: 0.0 ± 0.0
Met
2.287MetAla: 2.287 ± 0.04
0.153MetCys: 0.153 ± 0.009
1.529MetAsp: 1.529 ± 0.031
1.966MetGlu: 1.966 ± 0.039
0.984MetPhe: 0.984 ± 0.024
1.765MetGly: 1.765 ± 0.035
0.413MetHis: 0.413 ± 0.015
1.849MetIle: 1.849 ± 0.037
1.95MetLys: 1.95 ± 0.035
2.871MetLeu: 2.871 ± 0.051
0.836MetMet: 0.836 ± 0.022
1.366MetAsn: 1.366 ± 0.03
1.083MetPro: 1.083 ± 0.025
0.883MetGln: 0.883 ± 0.025
1.345MetArg: 1.345 ± 0.029
1.764MetSer: 1.764 ± 0.036
1.627MetThr: 1.627 ± 0.031
1.716MetVal: 1.716 ± 0.036
0.212MetTrp: 0.212 ± 0.009
0.74MetTyr: 0.74 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
2.73AsnAla: 2.73 ± 0.044
0.248AsnCys: 0.248 ± 0.013
1.681AsnAsp: 1.681 ± 0.037
2.415AsnGlu: 2.415 ± 0.042
1.305AsnPhe: 1.305 ± 0.033
3.15AsnGly: 3.15 ± 0.049
0.754AsnHis: 0.754 ± 0.022
2.088AsnIle: 2.088 ± 0.035
1.861AsnLys: 1.861 ± 0.04
3.347AsnLeu: 3.347 ± 0.045
0.958AsnMet: 0.958 ± 0.025
1.35AsnAsn: 1.35 ± 0.036
2.01AsnPro: 2.01 ± 0.038
1.321AsnGln: 1.321 ± 0.028
2.243AsnArg: 2.243 ± 0.039
1.91AsnSer: 1.91 ± 0.035
1.804AsnThr: 1.804 ± 0.031
2.337AsnVal: 2.337 ± 0.039
0.531AsnTrp: 0.531 ± 0.019
1.368AsnTyr: 1.368 ± 0.032
0.0AsnXaa: 0.0 ± 0.0
Pro
3.761ProAla: 3.761 ± 0.052
0.254ProCys: 0.254 ± 0.012
2.795ProAsp: 2.795 ± 0.043
3.974ProGlu: 3.974 ± 0.053
1.888ProPhe: 1.888 ± 0.037
3.604ProGly: 3.604 ± 0.061
0.862ProHis: 0.862 ± 0.021
2.147ProIle: 2.147 ± 0.034
1.885ProLys: 1.885 ± 0.036
4.146ProLeu: 4.146 ± 0.05
0.993ProMet: 0.993 ± 0.028
1.464ProAsn: 1.464 ± 0.03
1.483ProPro: 1.483 ± 0.031
1.422ProGln: 1.422 ± 0.026
1.634ProArg: 1.634 ± 0.032
2.478ProSer: 2.478 ± 0.041
1.85ProThr: 1.85 ± 0.031
3.547ProVal: 3.547 ± 0.048
0.541ProTrp: 0.541 ± 0.016
1.516ProTyr: 1.516 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
3.36GlnAla: 3.36 ± 0.06
0.177GlnCys: 0.177 ± 0.01
1.619GlnAsp: 1.619 ± 0.031
2.605GlnGlu: 2.605 ± 0.046
1.392GlnPhe: 1.392 ± 0.028
2.702GlnGly: 2.702 ± 0.042
0.692GlnHis: 0.692 ± 0.022
2.366GlnIle: 2.366 ± 0.037
1.877GlnLys: 1.877 ± 0.035
3.718GlnLeu: 3.718 ± 0.05
1.041GlnMet: 1.041 ± 0.024
1.296GlnAsn: 1.296 ± 0.03
1.496GlnPro: 1.496 ± 0.032
1.509GlnGln: 1.509 ± 0.034
1.993GlnArg: 1.993 ± 0.039
2.009GlnSer: 2.009 ± 0.035
1.887GlnThr: 1.887 ± 0.037
2.463GlnVal: 2.463 ± 0.038
0.462GlnTrp: 0.462 ± 0.019
1.182GlnTyr: 1.182 ± 0.029
0.0GlnXaa: 0.0 ± 0.0
Arg
3.729ArgAla: 3.729 ± 0.048
0.39ArgCys: 0.39 ± 0.017
2.763ArgAsp: 2.763 ± 0.04
4.649ArgGlu: 4.649 ± 0.065
2.449ArgPhe: 2.449 ± 0.037
3.513ArgGly: 3.513 ± 0.046
1.234ArgHis: 1.234 ± 0.037
3.881ArgIle: 3.881 ± 0.046
3.416ArgLys: 3.416 ± 0.042
6.164ArgLeu: 6.164 ± 0.066
1.732ArgMet: 1.732 ± 0.029
2.066ArgAsn: 2.066 ± 0.033
2.027ArgPro: 2.027 ± 0.03
2.259ArgGln: 2.259 ± 0.035
3.435ArgArg: 3.435 ± 0.053
3.212ArgSer: 3.212 ± 0.043
2.798ArgThr: 2.798 ± 0.038
3.523ArgVal: 3.523 ± 0.046
0.711ArgTrp: 0.711 ± 0.021
2.06ArgTyr: 2.06 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
5.043SerAla: 5.043 ± 0.062
0.411SerCys: 0.411 ± 0.017
2.865SerAsp: 2.865 ± 0.046
3.728SerGlu: 3.728 ± 0.057
2.898SerPhe: 2.898 ± 0.042
5.722SerGly: 5.722 ± 0.067
1.19SerHis: 1.19 ± 0.028
3.733SerIle: 3.733 ± 0.055
2.885SerLys: 2.885 ± 0.046
6.295SerLeu: 6.295 ± 0.069
1.666SerMet: 1.666 ± 0.03
1.879SerAsn: 1.879 ± 0.039
2.62SerPro: 2.62 ± 0.041
1.755SerGln: 1.755 ± 0.033
3.431SerArg: 3.431 ± 0.049
3.82SerSer: 3.82 ± 0.067
2.77SerThr: 2.77 ± 0.045
4.386SerVal: 4.386 ± 0.058
0.793SerTrp: 0.793 ± 0.025
2.073SerTyr: 2.073 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
4.789ThrAla: 4.789 ± 0.068
0.333ThrCys: 0.333 ± 0.015
2.695ThrAsp: 2.695 ± 0.044
3.342ThrGlu: 3.342 ± 0.051
2.174ThrPhe: 2.174 ± 0.034
4.849ThrGly: 4.849 ± 0.062
0.918ThrHis: 0.918 ± 0.025
3.189ThrIle: 3.189 ± 0.049
2.42ThrLys: 2.42 ± 0.038
5.49ThrLeu: 5.49 ± 0.061
1.289ThrMet: 1.289 ± 0.025
1.629ThrAsn: 1.629 ± 0.038
2.562ThrPro: 2.562 ± 0.041
1.437ThrGln: 1.437 ± 0.028
2.448ThrArg: 2.448 ± 0.036
2.982ThrSer: 2.982 ± 0.042
2.598ThrThr: 2.598 ± 0.042
4.491ThrVal: 4.491 ± 0.059
0.658ThrTrp: 0.658 ± 0.02
1.838ThrTyr: 1.838 ± 0.032
0.0ThrXaa: 0.0 ± 0.0
Val
5.252ValAla: 5.252 ± 0.057
0.603ValCys: 0.603 ± 0.019
3.555ValAsp: 3.555 ± 0.048
4.445ValGlu: 4.445 ± 0.051
3.029ValPhe: 3.029 ± 0.047
4.524ValGly: 4.524 ± 0.061
1.42ValHis: 1.42 ± 0.026
4.714ValIle: 4.714 ± 0.061
3.902ValLys: 3.902 ± 0.057
7.363ValLeu: 7.363 ± 0.076
1.994ValMet: 1.994 ± 0.035
2.703ValAsn: 2.703 ± 0.043
3.168ValPro: 3.168 ± 0.045
2.451ValGln: 2.451 ± 0.036
3.755ValArg: 3.755 ± 0.052
4.659ValSer: 4.659 ± 0.059
4.314ValThr: 4.314 ± 0.06
4.925ValVal: 4.925 ± 0.065
0.852ValTrp: 0.852 ± 0.021
2.474ValTyr: 2.474 ± 0.036
0.0ValXaa: 0.0 ± 0.0
Trp
0.848TrpAla: 0.848 ± 0.024
0.101TrpCys: 0.101 ± 0.007
0.692TrpAsp: 0.692 ± 0.019
0.856TrpGlu: 0.856 ± 0.024
0.575TrpPhe: 0.575 ± 0.02
0.888TrpGly: 0.888 ± 0.025
0.248TrpHis: 0.248 ± 0.011
0.895TrpIle: 0.895 ± 0.023
0.736TrpLys: 0.736 ± 0.022
1.415TrpLeu: 1.415 ± 0.033
0.436TrpMet: 0.436 ± 0.016
0.677TrpAsn: 0.677 ± 0.021
0.359TrpPro: 0.359 ± 0.016
0.444TrpGln: 0.444 ± 0.018
0.649TrpArg: 0.649 ± 0.02
0.839TrpSer: 0.839 ± 0.023
0.699TrpThr: 0.699 ± 0.026
0.804TrpVal: 0.804 ± 0.023
0.17TrpTrp: 0.17 ± 0.01
0.397TrpTyr: 0.397 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.732TyrAla: 2.732 ± 0.04
0.314TyrCys: 0.314 ± 0.014
1.873TyrAsp: 1.873 ± 0.036
2.369TyrGlu: 2.369 ± 0.039
1.669TyrPhe: 1.669 ± 0.04
2.82TyrGly: 2.82 ± 0.041
0.754TyrHis: 0.754 ± 0.023
2.098TyrIle: 2.098 ± 0.039
1.624TyrLys: 1.624 ± 0.034
3.477TyrLeu: 3.477 ± 0.054
0.859TyrMet: 0.859 ± 0.021
1.282TyrAsn: 1.282 ± 0.028
1.553TyrPro: 1.553 ± 0.034
1.151TyrGln: 1.151 ± 0.025
2.41TyrArg: 2.41 ± 0.04
1.99TyrSer: 1.99 ± 0.039
1.866TyrThr: 1.866 ± 0.036
2.249TyrVal: 2.249 ± 0.037
0.453TyrTrp: 0.453 ± 0.018
1.372TyrTyr: 1.372 ± 0.034
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5445 proteins (1785304 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski