Bacillus phage SalinJah

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Wphvirus; unclassified Wphvirus

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 290 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A173GC90|A0A173GC90_9CAUD Uncharacterized protein OS=Bacillus phage SalinJah OX=1837830 GN=SALINJAH_257 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.37KK3 pKa = 9.98VDD5 pKa = 3.9KK6 pKa = 9.18LTEE9 pKa = 4.12VIEE12 pKa = 4.29VVKK15 pKa = 10.42GLEE18 pKa = 4.09IIEE21 pKa = 4.93GGLEE25 pKa = 4.19VNWIEE30 pKa = 5.98SSDD33 pKa = 3.42EE34 pKa = 3.7WVINYY39 pKa = 10.09GDD41 pKa = 4.27VIIMDD46 pKa = 3.95GMSEE50 pKa = 4.04KK51 pKa = 10.28EE52 pKa = 3.78ANRR55 pKa = 11.84NYY57 pKa = 11.01DD58 pKa = 3.28KK59 pKa = 11.39LSAYY63 pKa = 8.71EE64 pKa = 4.05IKK66 pKa = 10.29IAQIDD71 pKa = 4.04LKK73 pKa = 10.62EE74 pKa = 3.82YY75 pKa = 9.97DD76 pKa = 3.81YY77 pKa = 11.14EE78 pKa = 4.89YY79 pKa = 11.65SMEE82 pKa = 4.12NNKK85 pKa = 10.18GEE87 pKa = 4.38SFTEE91 pKa = 3.7IALEE95 pKa = 4.14RR96 pKa = 11.84MVIDD100 pKa = 3.1SWDD103 pKa = 3.67SEE105 pKa = 4.49FEE107 pKa = 4.19TVAFEE112 pKa = 4.45VADD115 pKa = 4.07GATPIANYY123 pKa = 9.05EE124 pKa = 3.74IWEE127 pKa = 4.36VASNNYY133 pKa = 9.51EE134 pKa = 4.22SIEE137 pKa = 4.0EE138 pKa = 4.21NIEE141 pKa = 3.71EE142 pKa = 4.22GLIIIDD148 pKa = 4.49GEE150 pKa = 4.08LTRR153 pKa = 11.84NFMSSIAYY161 pKa = 6.41TLNRR165 pKa = 11.84AIEE168 pKa = 4.16VNKK171 pKa = 10.87NEE173 pKa = 3.82VLYY176 pKa = 10.98NYY178 pKa = 9.75IAYY181 pKa = 9.63RR182 pKa = 11.84VNKK185 pKa = 9.2MLLDD189 pKa = 3.54KK190 pKa = 10.76HH191 pKa = 5.44ATQMDD196 pKa = 3.88IEE198 pKa = 4.32GLEE201 pKa = 3.98QEE203 pKa = 4.42IEE205 pKa = 4.2NYY207 pKa = 10.5ADD209 pKa = 3.49EE210 pKa = 5.45AEE212 pKa = 3.99NSYY215 pKa = 11.65NEE217 pKa = 3.9FSYY220 pKa = 10.92IDD222 pKa = 4.52DD223 pKa = 3.7EE224 pKa = 4.5MFSQLEE230 pKa = 4.16EE231 pKa = 4.99FIDD234 pKa = 4.06SYY236 pKa = 11.41IAGLSMGEE244 pKa = 4.1SLNNQDD250 pKa = 3.94YY251 pKa = 11.32EE252 pKa = 4.53NIGDD256 pKa = 3.78VTVTEE261 pKa = 4.23GAMFIKK267 pKa = 10.26KK268 pKa = 10.43DD269 pKa = 3.3EE270 pKa = 4.62DD271 pKa = 3.78TDD273 pKa = 3.24KK274 pKa = 10.92SYY276 pKa = 11.66YY277 pKa = 8.52VVRR280 pKa = 11.84VCGNEE285 pKa = 3.16FDD287 pKa = 4.23GFHH290 pKa = 6.45VEE292 pKa = 4.86NMYY295 pKa = 11.04VDD297 pKa = 5.8LNDD300 pKa = 4.5GWIDD304 pKa = 3.25WYY306 pKa = 10.94AINDD310 pKa = 3.63YY311 pKa = 11.38AGIDD315 pKa = 3.78MDD317 pKa = 4.89ADD319 pKa = 3.67DD320 pKa = 4.56YY321 pKa = 11.87EE322 pKa = 4.49RR323 pKa = 11.84VNALISYY330 pKa = 10.25DD331 pKa = 4.03GIDD334 pKa = 3.64NFNPTVTDD342 pKa = 3.65CKK344 pKa = 10.69TPIDD348 pKa = 3.58LCGALRR354 pKa = 11.84EE355 pKa = 4.34HH356 pKa = 6.73GVNVVWSAEE365 pKa = 3.9HH366 pKa = 7.18NEE368 pKa = 4.01IQFF371 pKa = 3.59

Molecular weight: 42.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A173GBT9|A0A173GBT9_9CAUD Uncharacterized protein OS=Bacillus phage SalinJah OX=1837830 GN=SALINJAH_89 PE=4 SV=1
MM1 pKa = 7.16TRR3 pKa = 11.84HH4 pKa = 4.52TRR6 pKa = 11.84NRR8 pKa = 11.84QMLKK12 pKa = 9.15ATNGKK17 pKa = 8.53WGALFNRR24 pKa = 11.84KK25 pKa = 8.24VKK27 pKa = 10.6NVAIAEE33 pKa = 4.14LNKK36 pKa = 10.02MEE38 pKa = 3.96IHH40 pKa = 6.87LDD42 pKa = 3.68KK43 pKa = 10.98SFKK46 pKa = 9.02WKK48 pKa = 10.55RR49 pKa = 11.84EE50 pKa = 3.57FDD52 pKa = 3.95VKK54 pKa = 10.3STNHH58 pKa = 5.86LPNYY62 pKa = 8.99RR63 pKa = 11.84QRR65 pKa = 11.84QDD67 pKa = 3.25YY68 pKa = 9.4FQDD71 pKa = 3.76GFPTFYY77 pKa = 9.6ITTFAQISFKK87 pKa = 10.91RR88 pKa = 11.84KK89 pKa = 6.86TKK91 pKa = 8.94TAATVFITHH100 pKa = 6.47KK101 pKa = 10.58RR102 pKa = 11.84NGKK105 pKa = 7.23VRR107 pKa = 11.84RR108 pKa = 11.84TEE110 pKa = 3.87IYY112 pKa = 10.08NGPIFGWEE120 pKa = 4.23SVNNAA125 pKa = 3.66

Molecular weight: 14.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

290

0

290

49322

23

2006

170.1

19.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.174 ± 0.234

0.884 ± 0.073

6.547 ± 0.126

8.063 ± 0.292

3.946 ± 0.1

6.121 ± 0.23

1.926 ± 0.1

6.798 ± 0.123

7.767 ± 0.217

7.885 ± 0.168

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.246 ± 0.112

5.405 ± 0.16

2.816 ± 0.151

3.232 ± 0.106

4.398 ± 0.115

5.649 ± 0.133

6.322 ± 0.223

7.212 ± 0.177

1.123 ± 0.06

4.487 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski