Amino acid dipepetide frequency for Bacillus phage SalinJah

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.339AlaAla: 4.339 ± 0.611
0.426AlaCys: 0.426 ± 0.104
3.71AlaAsp: 3.71 ± 0.334
4.562AlaGlu: 4.562 ± 0.348
2.474AlaPhe: 2.474 ± 0.235
3.933AlaGly: 3.933 ± 0.489
1.237AlaHis: 1.237 ± 0.147
4.258AlaIle: 4.258 ± 0.308
4.643AlaLys: 4.643 ± 0.316
5.292AlaLeu: 5.292 ± 0.333
2.088AlaMet: 2.088 ± 0.212
3.224AlaAsn: 3.224 ± 0.29
1.865AlaPro: 1.865 ± 0.227
2.129AlaGln: 2.129 ± 0.228
2.717AlaArg: 2.717 ± 0.229
3.264AlaSer: 3.264 ± 0.392
3.933AlaThr: 3.933 ± 0.407
4.136AlaVal: 4.136 ± 0.278
0.75AlaTrp: 0.75 ± 0.115
2.757AlaTyr: 2.757 ± 0.233
0.0AlaXaa: 0.0 ± 0.0
Cys
0.304CysAla: 0.304 ± 0.092
0.142CysCys: 0.142 ± 0.058
0.831CysAsp: 0.831 ± 0.131
0.689CysGlu: 0.689 ± 0.112
0.466CysPhe: 0.466 ± 0.102
0.73CysGly: 0.73 ± 0.133
0.203CysHis: 0.203 ± 0.074
0.507CysIle: 0.507 ± 0.107
0.73CysLys: 0.73 ± 0.138
0.487CysLeu: 0.487 ± 0.092
0.284CysMet: 0.284 ± 0.082
0.406CysAsn: 0.406 ± 0.092
0.406CysPro: 0.406 ± 0.098
0.223CysGln: 0.223 ± 0.059
0.365CysArg: 0.365 ± 0.094
0.507CysSer: 0.507 ± 0.103
0.547CysThr: 0.547 ± 0.099
0.568CysVal: 0.568 ± 0.101
0.122CysTrp: 0.122 ± 0.054
0.324CysTyr: 0.324 ± 0.078
0.0CysXaa: 0.0 ± 0.0
Asp
4.177AspAla: 4.177 ± 0.307
0.73AspCys: 0.73 ± 0.117
4.278AspAsp: 4.278 ± 0.366
5.251AspGlu: 5.251 ± 0.336
3.001AspPhe: 3.001 ± 0.267
3.913AspGly: 3.913 ± 0.332
0.75AspHis: 0.75 ± 0.13
5.17AspIle: 5.17 ± 0.332
5.637AspLys: 5.637 ± 0.332
5.109AspLeu: 5.109 ± 0.392
2.149AspMet: 2.149 ± 0.256
2.98AspAsn: 2.98 ± 0.218
1.926AspPro: 1.926 ± 0.25
1.054AspGln: 1.054 ± 0.137
2.859AspArg: 2.859 ± 0.213
3.427AspSer: 3.427 ± 0.244
3.609AspThr: 3.609 ± 0.292
5.393AspVal: 5.393 ± 0.308
0.852AspTrp: 0.852 ± 0.133
3.204AspTyr: 3.204 ± 0.247
0.0AspXaa: 0.0 ± 0.0
Glu
4.42GluAla: 4.42 ± 0.346
0.689GluCys: 0.689 ± 0.139
4.805GluAsp: 4.805 ± 0.334
8.597GluGlu: 8.597 ± 0.681
3.791GluPhe: 3.791 ± 0.269
4.704GluGly: 4.704 ± 0.278
1.744GluHis: 1.744 ± 0.194
5.434GluIle: 5.434 ± 0.323
5.353GluLys: 5.353 ± 0.448
8.212GluLeu: 8.212 ± 0.446
3.244GluMet: 3.244 ± 0.265
3.406GluAsn: 3.406 ± 0.247
1.886GluPro: 1.886 ± 0.212
2.839GluGln: 2.839 ± 0.218
3.548GluArg: 3.548 ± 0.286
4.035GluSer: 4.035 ± 0.265
3.467GluThr: 3.467 ± 0.23
6.083GluVal: 6.083 ± 0.468
1.014GluTrp: 1.014 ± 0.151
3.366GluTyr: 3.366 ± 0.302
0.0GluXaa: 0.0 ± 0.0
Phe
2.23PheAla: 2.23 ± 0.248
0.385PheCys: 0.385 ± 0.087
3.082PheAsp: 3.082 ± 0.226
2.575PheGlu: 2.575 ± 0.197
1.419PhePhe: 1.419 ± 0.172
2.109PheGly: 2.109 ± 0.191
0.75PheHis: 0.75 ± 0.125
2.879PheIle: 2.879 ± 0.242
3.102PheLys: 3.102 ± 0.259
2.717PheLeu: 2.717 ± 0.248
1.561PheMet: 1.561 ± 0.143
2.757PheAsn: 2.757 ± 0.261
1.054PhePro: 1.054 ± 0.137
0.831PheGln: 0.831 ± 0.124
1.602PheArg: 1.602 ± 0.234
2.251PheSer: 2.251 ± 0.234
3.183PheThr: 3.183 ± 0.25
2.92PheVal: 2.92 ± 0.226
0.324PheTrp: 0.324 ± 0.083
1.723PheTyr: 1.723 ± 0.168
0.0PheXaa: 0.0 ± 0.0
Gly
3.366GlyAla: 3.366 ± 0.567
0.568GlyCys: 0.568 ± 0.114
3.244GlyAsp: 3.244 ± 0.224
4.765GlyGlu: 4.765 ± 0.323
2.717GlyPhe: 2.717 ± 0.231
5.15GlyGly: 5.15 ± 0.778
0.831GlyHis: 0.831 ± 0.16
4.339GlyIle: 4.339 ± 0.407
4.947GlyLys: 4.947 ± 0.419
4.319GlyLeu: 4.319 ± 0.34
1.865GlyMet: 1.865 ± 0.192
3.041GlyAsn: 3.041 ± 0.296
0.75GlyPro: 0.75 ± 0.124
1.865GlyGln: 1.865 ± 0.243
2.636GlyArg: 2.636 ± 0.188
3.447GlySer: 3.447 ± 0.362
3.933GlyThr: 3.933 ± 0.303
5.008GlyVal: 5.008 ± 0.268
0.993GlyTrp: 0.993 ± 0.126
3.427GlyTyr: 3.427 ± 0.277
0.0GlyXaa: 0.0 ± 0.0
His
0.993HisAla: 0.993 ± 0.154
0.203HisCys: 0.203 ± 0.07
1.196HisAsp: 1.196 ± 0.146
1.257HisGlu: 1.257 ± 0.155
0.649HisPhe: 0.649 ± 0.137
1.034HisGly: 1.034 ± 0.14
0.426HisHis: 0.426 ± 0.155
1.44HisIle: 1.44 ± 0.15
1.338HisLys: 1.338 ± 0.18
1.318HisLeu: 1.318 ± 0.158
0.75HisMet: 0.75 ± 0.13
1.135HisAsn: 1.135 ± 0.158
0.547HisPro: 0.547 ± 0.102
0.649HisGln: 0.649 ± 0.111
0.811HisArg: 0.811 ± 0.128
1.257HisSer: 1.257 ± 0.164
1.196HisThr: 1.196 ± 0.163
1.764HisVal: 1.764 ± 0.213
0.223HisTrp: 0.223 ± 0.063
1.075HisTyr: 1.075 ± 0.16
0.0HisXaa: 0.0 ± 0.0
Ile
4.562IleAla: 4.562 ± 0.332
0.71IleCys: 0.71 ± 0.122
5.19IleAsp: 5.19 ± 0.357
5.637IleGlu: 5.637 ± 0.375
1.825IlePhe: 1.825 ± 0.183
4.116IleGly: 4.116 ± 0.283
1.257IleHis: 1.257 ± 0.17
4.562IleIle: 4.562 ± 0.363
5.92IleLys: 5.92 ± 0.33
4.319IleLeu: 4.319 ± 0.34
2.068IleMet: 2.068 ± 0.23
3.548IleAsn: 3.548 ± 0.261
2.048IlePro: 2.048 ± 0.221
2.19IleGln: 2.19 ± 0.212
3.244IleArg: 3.244 ± 0.256
4.298IleSer: 4.298 ± 0.332
5.028IleThr: 5.028 ± 0.313
4.623IleVal: 4.623 ± 0.337
0.588IleTrp: 0.588 ± 0.107
2.251IleTyr: 2.251 ± 0.191
0.0IleXaa: 0.0 ± 0.0
Lys
5.049LysAla: 5.049 ± 0.425
0.507LysCys: 0.507 ± 0.108
5.17LysAsp: 5.17 ± 0.282
7.177LysGlu: 7.177 ± 0.498
2.311LysPhe: 2.311 ± 0.225
5.555LysGly: 5.555 ± 0.408
2.048LysHis: 2.048 ± 0.206
4.177LysIle: 4.177 ± 0.262
5.778LysLys: 5.778 ± 0.459
6.285LysLeu: 6.285 ± 0.323
2.818LysMet: 2.818 ± 0.234
3.994LysAsn: 3.994 ± 0.268
2.413LysPro: 2.413 ± 0.248
3.264LysGln: 3.264 ± 0.28
3.386LysArg: 3.386 ± 0.267
3.791LysSer: 3.791 ± 0.285
4.197LysThr: 4.197 ± 0.324
5.454LysVal: 5.454 ± 0.361
1.034LysTrp: 1.034 ± 0.13
3.264LysTyr: 3.264 ± 0.263
0.0LysXaa: 0.0 ± 0.0
Leu
5.211LeuAla: 5.211 ± 0.35
0.588LeuCys: 0.588 ± 0.11
5.799LeuAsp: 5.799 ± 0.361
7.542LeuGlu: 7.542 ± 0.493
2.676LeuPhe: 2.676 ± 0.229
4.4LeuGly: 4.4 ± 0.32
1.277LeuHis: 1.277 ± 0.163
4.826LeuIle: 4.826 ± 0.314
5.555LeuLys: 5.555 ± 0.333
5.677LeuLeu: 5.677 ± 0.419
2.169LeuMet: 2.169 ± 0.254
4.298LeuAsn: 4.298 ± 0.312
2.92LeuPro: 2.92 ± 0.247
2.757LeuGln: 2.757 ± 0.269
3.832LeuArg: 3.832 ± 0.328
4.562LeuSer: 4.562 ± 0.323
5.312LeuThr: 5.312 ± 0.32
5.758LeuVal: 5.758 ± 0.371
0.73LeuTrp: 0.73 ± 0.118
2.96LeuTyr: 2.96 ± 0.278
0.0LeuXaa: 0.0 ± 0.0
Met
1.825MetAla: 1.825 ± 0.195
0.345MetCys: 0.345 ± 0.091
1.886MetAsp: 1.886 ± 0.21
2.088MetGlu: 2.088 ± 0.212
1.44MetPhe: 1.44 ± 0.176
1.744MetGly: 1.744 ± 0.224
0.608MetHis: 0.608 ± 0.1
1.967MetIle: 1.967 ± 0.236
2.98MetLys: 2.98 ± 0.268
2.697MetLeu: 2.697 ± 0.216
0.993MetMet: 0.993 ± 0.16
1.967MetAsn: 1.967 ± 0.215
0.588MetPro: 0.588 ± 0.115
0.872MetGln: 0.872 ± 0.144
1.683MetArg: 1.683 ± 0.183
2.453MetSer: 2.453 ± 0.198
2.717MetThr: 2.717 ± 0.318
1.805MetVal: 1.805 ± 0.192
0.324MetTrp: 0.324 ± 0.078
1.48MetTyr: 1.48 ± 0.165
0.0MetXaa: 0.0 ± 0.0
Asn
3.163AsnAla: 3.163 ± 0.272
0.507AsnCys: 0.507 ± 0.108
2.899AsnAsp: 2.899 ± 0.246
3.427AsnGlu: 3.427 ± 0.282
2.028AsnPhe: 2.028 ± 0.189
4.075AsnGly: 4.075 ± 0.336
1.095AsnHis: 1.095 ± 0.171
3.812AsnIle: 3.812 ± 0.257
4.542AsnLys: 4.542 ± 0.322
3.751AsnLeu: 3.751 ± 0.317
2.068AsnMet: 2.068 ± 0.186
3.224AsnAsn: 3.224 ± 0.275
2.169AsnPro: 2.169 ± 0.219
1.825AsnGln: 1.825 ± 0.182
2.676AsnArg: 2.676 ± 0.229
2.778AsnSer: 2.778 ± 0.272
3.406AsnThr: 3.406 ± 0.306
3.548AsnVal: 3.548 ± 0.255
0.669AsnTrp: 0.669 ± 0.11
2.392AsnTyr: 2.392 ± 0.278
0.0AsnXaa: 0.0 ± 0.0
Pro
2.129ProAla: 2.129 ± 0.246
0.264ProCys: 0.264 ± 0.08
2.19ProAsp: 2.19 ± 0.255
2.778ProGlu: 2.778 ± 0.242
1.318ProPhe: 1.318 ± 0.17
0.0ProGly: 0.0 ± 0.0
0.547ProHis: 0.547 ± 0.102
1.906ProIle: 1.906 ± 0.175
1.987ProLys: 1.987 ± 0.247
1.723ProLeu: 1.723 ± 0.186
0.811ProMet: 0.811 ± 0.151
2.068ProAsn: 2.068 ± 0.23
0.649ProPro: 0.649 ± 0.141
0.811ProGln: 0.811 ± 0.133
1.135ProArg: 1.135 ± 0.133
1.845ProSer: 1.845 ± 0.239
2.555ProThr: 2.555 ± 0.34
1.723ProVal: 1.723 ± 0.204
0.243ProTrp: 0.243 ± 0.061
1.48ProTyr: 1.48 ± 0.196
0.0ProXaa: 0.0 ± 0.0
Gln
1.967GlnAla: 1.967 ± 0.264
0.284GlnCys: 0.284 ± 0.078
1.967GlnAsp: 1.967 ± 0.188
2.595GlnGlu: 2.595 ± 0.217
1.156GlnPhe: 1.156 ± 0.153
1.987GlnGly: 1.987 ± 0.208
0.669GlnHis: 0.669 ± 0.098
2.169GlnIle: 2.169 ± 0.208
1.906GlnLys: 1.906 ± 0.216
3.041GlnLeu: 3.041 ± 0.253
1.135GlnMet: 1.135 ± 0.166
1.419GlnAsn: 1.419 ± 0.169
0.973GlnPro: 0.973 ± 0.156
1.298GlnGln: 1.298 ± 0.21
1.561GlnArg: 1.561 ± 0.168
1.865GlnSer: 1.865 ± 0.258
1.642GlnThr: 1.642 ± 0.176
2.616GlnVal: 2.616 ± 0.252
0.426GlnTrp: 0.426 ± 0.088
1.622GlnTyr: 1.622 ± 0.19
0.0GlnXaa: 0.0 ± 0.0
Arg
3.041ArgAla: 3.041 ± 0.268
0.324ArgCys: 0.324 ± 0.082
2.717ArgAsp: 2.717 ± 0.27
3.325ArgGlu: 3.325 ± 0.32
1.967ArgPhe: 1.967 ± 0.185
2.737ArgGly: 2.737 ± 0.236
0.872ArgHis: 0.872 ± 0.151
3.041ArgIle: 3.041 ± 0.286
3.224ArgLys: 3.224 ± 0.282
4.035ArgLeu: 4.035 ± 0.328
1.541ArgMet: 1.541 ± 0.177
2.595ArgAsn: 2.595 ± 0.251
1.196ArgPro: 1.196 ± 0.169
1.5ArgGln: 1.5 ± 0.199
1.703ArgArg: 1.703 ± 0.239
1.764ArgSer: 1.764 ± 0.219
2.534ArgThr: 2.534 ± 0.199
3.447ArgVal: 3.447 ± 0.239
0.446ArgTrp: 0.446 ± 0.09
1.967ArgTyr: 1.967 ± 0.185
0.0ArgXaa: 0.0 ± 0.0
Ser
3.751SerAla: 3.751 ± 0.368
0.426SerCys: 0.426 ± 0.104
3.528SerAsp: 3.528 ± 0.234
3.954SerGlu: 3.954 ± 0.336
2.595SerPhe: 2.595 ± 0.235
3.812SerGly: 3.812 ± 0.423
0.852SerHis: 0.852 ± 0.119
4.075SerIle: 4.075 ± 0.288
4.278SerLys: 4.278 ± 0.334
4.521SerLeu: 4.521 ± 0.278
1.865SerMet: 1.865 ± 0.197
3.122SerAsn: 3.122 ± 0.286
1.399SerPro: 1.399 ± 0.202
1.744SerGln: 1.744 ± 0.198
2.595SerArg: 2.595 ± 0.224
3.589SerSer: 3.589 ± 0.442
3.224SerThr: 3.224 ± 0.268
3.548SerVal: 3.548 ± 0.258
0.73SerTrp: 0.73 ± 0.114
2.879SerTyr: 2.879 ± 0.255
0.0SerXaa: 0.0 ± 0.0
Thr
4.116ThrAla: 4.116 ± 0.424
0.466ThrCys: 0.466 ± 0.093
3.913ThrAsp: 3.913 ± 0.252
4.238ThrGlu: 4.238 ± 0.333
3.143ThrPhe: 3.143 ± 0.27
4.4ThrGly: 4.4 ± 0.375
1.419ThrHis: 1.419 ± 0.164
4.096ThrIle: 4.096 ± 0.303
4.765ThrLys: 4.765 ± 0.306
5.292ThrLeu: 5.292 ± 0.317
1.581ThrMet: 1.581 ± 0.168
3.589ThrAsn: 3.589 ± 0.248
2.291ThrPro: 2.291 ± 0.231
1.845ThrGln: 1.845 ± 0.22
2.818ThrArg: 2.818 ± 0.222
3.366ThrSer: 3.366 ± 0.315
3.954ThrThr: 3.954 ± 0.437
4.866ThrVal: 4.866 ± 0.324
0.487ThrTrp: 0.487 ± 0.102
2.575ThrTyr: 2.575 ± 0.26
0.0ThrXaa: 0.0 ± 0.0
Val
4.177ValAla: 4.177 ± 0.285
0.811ValCys: 0.811 ± 0.119
5.028ValAsp: 5.028 ± 0.343
5.576ValGlu: 5.576 ± 0.35
2.453ValPhe: 2.453 ± 0.238
3.548ValGly: 3.548 ± 0.285
1.419ValHis: 1.419 ± 0.178
5.15ValIle: 5.15 ± 0.382
6.225ValLys: 6.225 ± 0.368
5.596ValLeu: 5.596 ± 0.326
1.845ValMet: 1.845 ± 0.207
3.933ValAsn: 3.933 ± 0.327
1.906ValPro: 1.906 ± 0.258
2.595ValGln: 2.595 ± 0.231
2.595ValArg: 2.595 ± 0.256
4.643ValSer: 4.643 ± 0.32
5.19ValThr: 5.19 ± 0.378
5.312ValVal: 5.312 ± 0.388
0.608ValTrp: 0.608 ± 0.121
3.508ValTyr: 3.508 ± 0.321
0.0ValXaa: 0.0 ± 0.0
Trp
0.629TrpAla: 0.629 ± 0.11
0.061TrpCys: 0.061 ± 0.038
0.973TrpAsp: 0.973 ± 0.123
0.973TrpGlu: 0.973 ± 0.145
0.446TrpPhe: 0.446 ± 0.099
0.73TrpGly: 0.73 ± 0.109
0.304TrpHis: 0.304 ± 0.07
0.75TrpIle: 0.75 ± 0.128
0.892TrpLys: 0.892 ± 0.135
0.953TrpLeu: 0.953 ± 0.12
0.243TrpMet: 0.243 ± 0.071
0.568TrpAsn: 0.568 ± 0.106
0.0TrpPro: 0.0 ± 0.0
0.547TrpGln: 0.547 ± 0.103
0.264TrpArg: 0.264 ± 0.072
0.608TrpSer: 0.608 ± 0.112
0.77TrpThr: 0.77 ± 0.163
0.791TrpVal: 0.791 ± 0.122
0.162TrpTrp: 0.162 ± 0.054
0.568TrpTyr: 0.568 ± 0.092
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.291TyrAla: 2.291 ± 0.228
0.406TyrCys: 0.406 ± 0.084
3.062TyrAsp: 3.062 ± 0.247
3.629TyrGlu: 3.629 ± 0.302
1.581TyrPhe: 1.581 ± 0.21
2.534TyrGly: 2.534 ± 0.261
0.953TyrHis: 0.953 ± 0.153
3.427TyrIle: 3.427 ± 0.299
3.873TyrLys: 3.873 ± 0.262
3.508TyrLeu: 3.508 ± 0.288
1.358TyrMet: 1.358 ± 0.192
2.778TyrAsn: 2.778 ± 0.213
1.176TyrPro: 1.176 ± 0.156
1.48TyrGln: 1.48 ± 0.181
1.946TyrArg: 1.946 ± 0.197
2.737TyrSer: 2.737 ± 0.19
2.818TyrThr: 2.818 ± 0.246
2.757TyrVal: 2.757 ± 0.234
0.507TyrTrp: 0.507 ± 0.093
2.048TyrTyr: 2.048 ± 0.233
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 290 proteins (49322 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski