Amino acid dipepetide frequency for Caulobacteraceae bacterium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.128AlaAla: 21.128 ± 0.211	1.205AlaCys: 1.205 ± 0.033	8.018AlaAsp: 8.018 ± 0.088	7.719AlaGlu: 7.719 ± 0.105	4.855AlaPhe: 4.855 ± 0.071	13.439AlaGly: 13.439 ± 0.143	2.146AlaHis: 2.146 ± 0.042	5.766AlaIle: 5.766 ± 0.072	3.892AlaLys: 3.892 ± 0.076	14.344AlaLeu: 14.344 ± 0.134	3.624AlaMet: 3.624 ± 0.06	2.961AlaAsn: 2.961 ± 0.067	7.06AlaPro: 7.06 ± 0.101	4.293AlaGln: 4.293 ± 0.067	10.031AlaArg: 10.031 ± 0.114	6.415AlaSer: 6.415 ± 0.077	6.187AlaThr: 6.187 ± 0.096	9.802AlaVal: 9.802 ± 0.103	2.123AlaTrp: 2.123 ± 0.044	2.801AlaTyr: 2.801 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	1.049CysAla: 1.049 ± 0.032	0.075CysCys: 0.075 ± 0.007	0.476CysAsp: 0.476 ± 0.022	0.407CysGlu: 0.407 ± 0.019	0.25CysPhe: 0.25 ± 0.014	0.874CysGly: 0.874 ± 0.029	0.171CysHis: 0.171 ± 0.013	0.322CysIle: 0.322 ± 0.016	0.178CysLys: 0.178 ± 0.011	0.787CysLeu: 0.787 ± 0.025	0.139CysMet: 0.139 ± 0.011	0.183CysAsn: 0.183 ± 0.013	0.439CysPro: 0.439 ± 0.021	0.223CysGln: 0.223 ± 0.014	0.518CysArg: 0.518 ± 0.024	0.389CysSer: 0.389 ± 0.019	0.364CysThr: 0.364 ± 0.019	0.551CysVal: 0.551 ± 0.022	0.098CysTrp: 0.098 ± 0.009	0.147CysTyr: 0.147 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.241AspAla: 7.241 ± 0.097	0.477AspCys: 0.477 ± 0.021	3.437AspAsp: 3.437 ± 0.073	3.206AspGlu: 3.206 ± 0.06	2.203AspPhe: 2.203 ± 0.044	6.276AspGly: 6.276 ± 0.096	1.217AspHis: 1.217 ± 0.034	2.872AspIle: 2.872 ± 0.054	1.685AspLys: 1.685 ± 0.043	6.492AspLeu: 6.492 ± 0.077	1.264AspMet: 1.264 ± 0.029	1.332AspAsn: 1.332 ± 0.039	4.044AspPro: 4.044 ± 0.06	1.889AspGln: 1.889 ± 0.039	4.694AspArg: 4.694 ± 0.065	2.386AspSer: 2.386 ± 0.062	2.659AspThr: 2.659 ± 0.077	4.05AspVal: 4.05 ± 0.068	1.134AspTrp: 1.134 ± 0.034	1.606AspTyr: 1.606 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	8.701GluAla: 8.701 ± 0.108	0.268GluCys: 0.268 ± 0.016	2.95GluAsp: 2.95 ± 0.047	2.702GluGlu: 2.702 ± 0.055	1.448GluPhe: 1.448 ± 0.039	4.829GluGly: 4.829 ± 0.066	0.998GluHis: 0.998 ± 0.031	2.777GluIle: 2.777 ± 0.055	1.829GluLys: 1.829 ± 0.046	4.611GluLeu: 4.611 ± 0.074	1.321GluMet: 1.321 ± 0.037	1.167GluAsn: 1.167 ± 0.034	2.851GluPro: 2.851 ± 0.054	1.863GluGln: 1.863 ± 0.034	4.391GluArg: 4.391 ± 0.067	2.064GluSer: 2.064 ± 0.041	3.614GluThr: 3.614 ± 0.053	3.761GluVal: 3.761 ± 0.064	0.602GluTrp: 0.602 ± 0.023	0.844GluTyr: 0.844 ± 0.028	0.0GluXaa: 0.0 ± 0.0
Phe	4.493PheAla: 4.493 ± 0.069	0.349PheCys: 0.349 ± 0.017	2.642PheAsp: 2.642 ± 0.054	2.226PheGlu: 2.226 ± 0.048	1.189PhePhe: 1.189 ± 0.032	3.69PheGly: 3.69 ± 0.056	0.66PheHis: 0.66 ± 0.026	1.39PheIle: 1.39 ± 0.037	1.097PheLys: 1.097 ± 0.031	3.06PheLeu: 3.06 ± 0.066	0.747PheMet: 0.747 ± 0.025	1.06PheAsn: 1.06 ± 0.034	1.441PhePro: 1.441 ± 0.036	1.038PheGln: 1.038 ± 0.029	2.032PheArg: 2.032 ± 0.045	2.03PheSer: 2.03 ± 0.041	2.016PheThr: 2.016 ± 0.053	2.538PheVal: 2.538 ± 0.045	0.542PheTrp: 0.542 ± 0.024	0.843PheTyr: 0.843 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	11.913GlyAla: 11.913 ± 0.15	0.789GlyCys: 0.789 ± 0.031	5.729GlyAsp: 5.729 ± 0.12	5.461GlyGlu: 5.461 ± 0.082	3.679GlyPhe: 3.679 ± 0.065	9.71GlyGly: 9.71 ± 0.164	1.729GlyHis: 1.729 ± 0.046	3.412GlyIle: 3.412 ± 0.072	3.265GlyLys: 3.265 ± 0.063	10.342GlyLeu: 10.342 ± 0.128	2.277GlyMet: 2.277 ± 0.048	2.021GlyAsn: 2.021 ± 0.07	4.725GlyPro: 4.725 ± 0.07	3.366GlyGln: 3.366 ± 0.062	6.92GlyArg: 6.92 ± 0.104	4.599GlySer: 4.599 ± 0.08	3.849GlyThr: 3.849 ± 0.087	7.667GlyVal: 7.667 ± 0.094	1.748GlyTrp: 1.748 ± 0.045	2.356GlyTyr: 2.356 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	2.126HisAla: 2.126 ± 0.043	0.176HisCys: 0.176 ± 0.013	1.097HisAsp: 1.097 ± 0.033	0.893HisGlu: 0.893 ± 0.028	0.648HisPhe: 0.648 ± 0.022	1.822HisGly: 1.822 ± 0.039	0.439HisHis: 0.439 ± 0.024	0.738HisIle: 0.738 ± 0.027	0.397HisLys: 0.397 ± 0.018	1.717HisLeu: 1.717 ± 0.042	0.432HisMet: 0.432 ± 0.019	0.381HisAsn: 0.381 ± 0.015	1.264HisPro: 1.264 ± 0.038	0.502HisGln: 0.502 ± 0.022	1.288HisArg: 1.288 ± 0.037	0.737HisSer: 0.737 ± 0.023	0.72HisThr: 0.72 ± 0.025	1.183HisVal: 1.183 ± 0.031	0.313HisTrp: 0.313 ± 0.018	0.476HisTyr: 0.476 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	6.42IleAla: 6.42 ± 0.084	0.399IleCys: 0.399 ± 0.019	3.337IleAsp: 3.337 ± 0.052	3.046IleGlu: 3.046 ± 0.056	1.333IlePhe: 1.333 ± 0.038	4.484IleGly: 4.484 ± 0.065	0.763IleHis: 0.763 ± 0.025	1.747IleIle: 1.747 ± 0.043	1.221IleLys: 1.221 ± 0.036	4.122IleLeu: 4.122 ± 0.073	0.814IleMet: 0.814 ± 0.028	1.253IleAsn: 1.253 ± 0.043	2.015IlePro: 2.015 ± 0.039	1.199IleGln: 1.199 ± 0.034	2.733IleArg: 2.733 ± 0.048	2.259IleSer: 2.259 ± 0.043	2.442IleThr: 2.442 ± 0.053	3.603IleVal: 3.603 ± 0.066	0.547IleTrp: 0.547 ± 0.023	0.914IleTyr: 0.914 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	4.969LysAla: 4.969 ± 0.078	0.121LysCys: 0.121 ± 0.012	1.782LysAsp: 1.782 ± 0.047	1.211LysGlu: 1.211 ± 0.042	0.84LysPhe: 0.84 ± 0.026	2.906LysGly: 2.906 ± 0.063	0.476LysHis: 0.476 ± 0.021	1.332LysIle: 1.332 ± 0.04	0.985LysLys: 0.985 ± 0.04	2.862LysLeu: 2.862 ± 0.052	0.675LysMet: 0.675 ± 0.027	0.611LysAsn: 0.611 ± 0.024	2.101LysPro: 2.101 ± 0.051	0.82LysGln: 0.82 ± 0.027	2.029LysArg: 2.029 ± 0.047	1.499LysSer: 1.499 ± 0.042	1.902LysThr: 1.902 ± 0.044	2.452LysVal: 2.452 ± 0.048	0.335LysTrp: 0.335 ± 0.018	0.546LysTyr: 0.546 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	15.045LeuAla: 15.045 ± 0.164	0.797LeuCys: 0.797 ± 0.026	6.241LeuAsp: 6.241 ± 0.089	5.205LeuGlu: 5.205 ± 0.08	3.468LeuPhe: 3.468 ± 0.068	8.825LeuGly: 8.825 ± 0.096	1.606LeuHis: 1.606 ± 0.044	4.964LeuIle: 4.964 ± 0.078	3.782LeuLys: 3.782 ± 0.077	9.218LeuLeu: 9.218 ± 0.143	2.338LeuMet: 2.338 ± 0.044	2.559LeuAsn: 2.559 ± 0.053	5.199LeuPro: 5.199 ± 0.082	2.652LeuGln: 2.652 ± 0.055	6.413LeuArg: 6.413 ± 0.08	6.06LeuSer: 6.06 ± 0.08	6.348LeuThr: 6.348 ± 0.088	7.212LeuVal: 7.212 ± 0.095	1.28LeuTrp: 1.28 ± 0.038	2.074LeuTyr: 2.074 ± 0.04	0.0LeuXaa: 0.0 ± 0.0
Met	3.389MetAla: 3.389 ± 0.063	0.124MetCys: 0.124 ± 0.01	1.27MetAsp: 1.27 ± 0.03	0.98MetGlu: 0.98 ± 0.028	0.66MetPhe: 0.66 ± 0.023	1.941MetGly: 1.941 ± 0.046	0.344MetHis: 0.344 ± 0.019	1.197MetIle: 1.197 ± 0.035	0.896MetLys: 0.896 ± 0.024	2.21MetLeu: 2.21 ± 0.043	0.592MetMet: 0.592 ± 0.022	0.635MetAsn: 0.635 ± 0.024	1.294MetPro: 1.294 ± 0.032	0.674MetGln: 0.674 ± 0.022	1.656MetArg: 1.656 ± 0.037	1.563MetSer: 1.563 ± 0.038	1.973MetThr: 1.973 ± 0.04	1.522MetVal: 1.522 ± 0.035	0.208MetTrp: 0.208 ± 0.014	0.269MetTyr: 0.269 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.067AsnAla: 3.067 ± 0.074	0.178AsnCys: 0.178 ± 0.013	1.507AsnAsp: 1.507 ± 0.066	0.939AsnGlu: 0.939 ± 0.028	0.838AsnPhe: 0.838 ± 0.028	2.49AsnGly: 2.49 ± 0.079	0.439AsnHis: 0.439 ± 0.02	1.141AsnIle: 1.141 ± 0.035	0.498AsnLys: 0.498 ± 0.022	2.526AsnLeu: 2.526 ± 0.051	0.508AsnMet: 0.508 ± 0.021	0.649AsnAsn: 0.649 ± 0.031	1.69AsnPro: 1.69 ± 0.037	0.625AsnGln: 0.625 ± 0.024	1.595AsnArg: 1.595 ± 0.044	1.067AsnSer: 1.067 ± 0.037	1.167AsnThr: 1.167 ± 0.039	1.767AsnVal: 1.767 ± 0.051	0.347AsnTrp: 0.347 ± 0.019	0.552AsnTyr: 0.552 ± 0.024	0.0AsnXaa: 0.0 ± 0.0
Pro	7.944ProAla: 7.944 ± 0.113	0.297ProCys: 0.297 ± 0.016	3.877ProAsp: 3.877 ± 0.061	3.549ProGlu: 3.549 ± 0.056	2.001ProPhe: 2.001 ± 0.047	5.456ProGly: 5.456 ± 0.08	0.932ProHis: 0.932 ± 0.031	2.188ProIle: 2.188 ± 0.046	1.742ProLys: 1.742 ± 0.042	4.886ProLeu: 4.886 ± 0.072	1.287ProMet: 1.287 ± 0.034	1.234ProAsn: 1.234 ± 0.036	3.399ProPro: 3.399 ± 0.077	1.676ProGln: 1.676 ± 0.041	3.172ProArg: 3.172 ± 0.064	2.573ProSer: 2.573 ± 0.05	2.806ProThr: 2.806 ± 0.056	4.411ProVal: 4.411 ± 0.065	0.813ProTrp: 0.813 ± 0.028	1.105ProTyr: 1.105 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	4.848GlnAla: 4.848 ± 0.073	0.173GlnCys: 0.173 ± 0.013	1.524GlnAsp: 1.524 ± 0.038	1.248GlnGlu: 1.248 ± 0.034	0.901GlnPhe: 0.901 ± 0.032	2.782GlnGly: 2.782 ± 0.056	0.488GlnHis: 0.488 ± 0.02	1.475GlnIle: 1.475 ± 0.044	0.882GlnLys: 0.882 ± 0.03	2.799GlnLeu: 2.799 ± 0.05	0.786GlnMet: 0.786 ± 0.029	0.674GlnAsn: 0.674 ± 0.026	1.856GlnPro: 1.856 ± 0.048	1.017GlnGln: 1.017 ± 0.034	2.154GlnArg: 2.154 ± 0.045	1.539GlnSer: 1.539 ± 0.036	1.812GlnThr: 1.812 ± 0.036	2.437GlnVal: 2.437 ± 0.051	0.389GlnTrp: 0.389 ± 0.018	0.539GlnTyr: 0.539 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	8.511ArgAla: 8.511 ± 0.092	0.475ArgCys: 0.475 ± 0.022	3.95ArgAsp: 3.95 ± 0.063	3.824ArgGlu: 3.824 ± 0.06	2.79ArgPhe: 2.79 ± 0.05	5.197ArgGly: 5.197 ± 0.075	1.349ArgHis: 1.349 ± 0.037	3.513ArgIle: 3.513 ± 0.054	2.152ArgLys: 2.152 ± 0.045	8.64ArgLeu: 8.64 ± 0.107	1.805ArgMet: 1.805 ± 0.042	1.553ArgAsn: 1.553 ± 0.035	4.227ArgPro: 4.227 ± 0.074	2.447ArgGln: 2.447 ± 0.048	5.999ArgArg: 5.999 ± 0.101	3.196ArgSer: 3.196 ± 0.056	3.517ArgThr: 3.517 ± 0.055	4.655ArgVal: 4.655 ± 0.07	1.185ArgTrp: 1.185 ± 0.033	1.649ArgTyr: 1.649 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	6.136SerAla: 6.136 ± 0.084	0.344SerCys: 0.344 ± 0.018	2.913SerAsp: 2.913 ± 0.057	2.331SerGlu: 2.331 ± 0.039	1.961SerPhe: 1.961 ± 0.043	5.421SerGly: 5.421 ± 0.094	0.93SerHis: 0.93 ± 0.029	2.143SerIle: 2.143 ± 0.046	1.438SerLys: 1.438 ± 0.042	5.271SerLeu: 5.271 ± 0.071	1.108SerMet: 1.108 ± 0.032	1.238SerAsn: 1.238 ± 0.03	3.079SerPro: 3.079 ± 0.056	1.496SerGln: 1.496 ± 0.039	3.502SerArg: 3.502 ± 0.057	2.459SerSer: 2.459 ± 0.059	2.374SerThr: 2.374 ± 0.051	3.56SerVal: 3.56 ± 0.052	0.739SerTrp: 0.739 ± 0.028	1.184SerTyr: 1.184 ± 0.037	0.0SerXaa: 0.0 ± 0.0
Thr	7.121ThrAla: 7.121 ± 0.095	0.382ThrCys: 0.382 ± 0.017	2.979ThrAsp: 2.979 ± 0.061	2.265ThrGlu: 2.265 ± 0.043	1.947ThrPhe: 1.947 ± 0.043	5.669ThrGly: 5.669 ± 0.095	0.861ThrHis: 0.861 ± 0.026	2.274ThrIle: 2.274 ± 0.05	1.153ThrLys: 1.153 ± 0.036	6.141ThrLeu: 6.141 ± 0.089	1.022ThrMet: 1.022 ± 0.03	1.184ThrAsn: 1.184 ± 0.04	3.849ThrPro: 3.849 ± 0.06	1.402ThrGln: 1.402 ± 0.035	3.271ThrArg: 3.271 ± 0.06	2.507ThrSer: 2.507 ± 0.052	2.738ThrThr: 2.738 ± 0.054	4.403ThrVal: 4.403 ± 0.099	0.754ThrTrp: 0.754 ± 0.027	1.181ThrTyr: 1.181 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	9.527ValAla: 9.527 ± 0.106	0.7ValCys: 0.7 ± 0.026	4.227ValAsp: 4.227 ± 0.071	4.56ValGlu: 4.56 ± 0.062	2.685ValPhe: 2.685 ± 0.054	6.305ValGly: 6.305 ± 0.084	1.182ValHis: 1.182 ± 0.034	3.871ValIle: 3.871 ± 0.065	2.302ValLys: 2.302 ± 0.049	7.46ValLeu: 7.46 ± 0.096	1.848ValMet: 1.848 ± 0.046	1.902ValAsn: 1.902 ± 0.052	3.011ValPro: 3.011 ± 0.049	2.047ValGln: 2.047 ± 0.046	5.085ValArg: 5.085 ± 0.072	4.253ValSer: 4.253 ± 0.058	4.623ValThr: 4.623 ± 0.081	6.128ValVal: 6.128 ± 0.083	1.123ValTrp: 1.123 ± 0.03	1.379ValTyr: 1.379 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	1.583TrpAla: 1.583 ± 0.046	0.127TrpCys: 0.127 ± 0.01	0.748TrpAsp: 0.748 ± 0.027	0.607TrpGlu: 0.607 ± 0.025	0.586TrpPhe: 0.586 ± 0.023	1.097TrpGly: 1.097 ± 0.029	0.255TrpHis: 0.255 ± 0.015	0.736TrpIle: 0.736 ± 0.025	0.561TrpLys: 0.561 ± 0.023	1.731TrpLeu: 1.731 ± 0.041	0.402TrpMet: 0.402 ± 0.02	0.428TrpAsn: 0.428 ± 0.018	0.778TrpPro: 0.778 ± 0.028	0.427TrpGln: 0.427 ± 0.02	1.429TrpArg: 1.429 ± 0.034	0.915TrpSer: 0.915 ± 0.027	1.071TrpThr: 1.071 ± 0.027	0.87TrpVal: 0.87 ± 0.032	0.265TrpTrp: 0.265 ± 0.016	0.279TrpTyr: 0.279 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.6TyrAla: 2.6 ± 0.049	0.185TyrCys: 0.185 ± 0.014	1.459TyrAsp: 1.459 ± 0.04	1.211TyrGlu: 1.211 ± 0.029	0.802TyrPhe: 0.802 ± 0.025	2.344TyrGly: 2.344 ± 0.052	0.362TyrHis: 0.362 ± 0.018	0.795TyrIle: 0.795 ± 0.03	0.499TyrLys: 0.499 ± 0.02	2.068TyrLeu: 2.068 ± 0.042	0.407TyrMet: 0.407 ± 0.02	0.565TyrAsn: 0.565 ± 0.023	1.026TyrPro: 1.026 ± 0.032	0.665TyrGln: 0.665 ± 0.022	1.722TyrArg: 1.722 ± 0.038	1.118TyrSer: 1.118 ± 0.034	0.919TyrThr: 0.919 ± 0.037	1.645TyrVal: 1.645 ± 0.044	0.35TyrTrp: 0.35 ± 0.017	0.535TyrTyr: 0.535 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4191 proteins (1190268 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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