Thermoclostridium stercorarium (strain ATCC 35414 / DSM 8532 / NCIMB 11754) (Clostridium stercorarium)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Thermoclostridium; Thermoclostridium stercorarium; Thermoclostridium stercorarium subsp. stercorarium

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2669 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L7VTA6|L7VTA6_THES1 30S ribosomal protein S10 OS=Thermoclostridium stercorarium (strain ATCC 35414 / DSM 8532 / NCIMB 11754) OX=1121335 GN=rpsJ PE=3 SV=1
MM1 pKa = 7.71KK2 pKa = 10.32KK3 pKa = 10.35NKK5 pKa = 9.09TPFIIAAAVLIAVGIAVLVFINQGNGNEE33 pKa = 4.43NIGDD37 pKa = 3.89KK38 pKa = 10.69NVSSNADD45 pKa = 3.25EE46 pKa = 4.71NGDD49 pKa = 3.68VKK51 pKa = 10.97DD52 pKa = 3.84TGEE55 pKa = 4.01TGQNNGNIGEE65 pKa = 4.2RR66 pKa = 11.84KK67 pKa = 9.73GYY69 pKa = 8.09YY70 pKa = 9.42FEE72 pKa = 4.39YY73 pKa = 10.23KK74 pKa = 10.34GVEE77 pKa = 3.38IGINDD82 pKa = 3.49EE83 pKa = 3.89AAPIIEE89 pKa = 4.35ALGEE93 pKa = 3.81PMNYY97 pKa = 9.56FEE99 pKa = 5.75APSCAFEE106 pKa = 4.86GMDD109 pKa = 4.86KK110 pKa = 10.23IYY112 pKa = 10.44SYY114 pKa = 11.59SGFEE118 pKa = 3.74FTTYY122 pKa = 10.36TKK124 pKa = 10.61DD125 pKa = 3.2GKK127 pKa = 10.62DD128 pKa = 3.66YY129 pKa = 10.68IASLVFLDD137 pKa = 3.95DD138 pKa = 3.42TVTTRR143 pKa = 11.84EE144 pKa = 4.52GITLNATLDD153 pKa = 3.96DD154 pKa = 4.54VIATYY159 pKa = 10.71GSDD162 pKa = 3.58YY163 pKa = 10.45EE164 pKa = 4.27QSFSKK169 pKa = 10.88YY170 pKa = 10.0SYY172 pKa = 11.26SDD174 pKa = 3.72GNCVLSFIIEE184 pKa = 4.07NGEE187 pKa = 4.3VVSVEE192 pKa = 3.55YY193 pKa = 11.57SMISEE198 pKa = 4.43EE199 pKa = 4.13NTEE202 pKa = 4.23GG203 pKa = 3.75

Molecular weight: 22.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L7VSS8|L7VSS8_THES1 Phosphoribosylaminoimidazole-succinocarboxamide synthase OS=Thermoclostridium stercorarium (strain ATCC 35414 / DSM 8532 / NCIMB 11754) OX=1121335 GN=purC PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 8.61KK9 pKa = 7.7RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 7.92VEE15 pKa = 3.7HH16 pKa = 6.38GFRR19 pKa = 11.84KK20 pKa = 9.82RR21 pKa = 11.84MKK23 pKa = 8.53TRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 7.47VLKK32 pKa = 10.12RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.23GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2669

0

2669

856082

30

1808

320.8

36.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.651 ± 0.044

1.191 ± 0.021

5.344 ± 0.034

7.395 ± 0.048

4.457 ± 0.035

6.874 ± 0.036

1.562 ± 0.021

8.423 ± 0.043

7.106 ± 0.037

9.01 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.712 ± 0.02

5.036 ± 0.031

3.655 ± 0.026

2.577 ± 0.029

4.858 ± 0.033

5.707 ± 0.039

5.131 ± 0.032

7.164 ± 0.037

0.935 ± 0.019

4.211 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski