Amino acid dipepetide frequency for Ralstonia phage RP13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.87AlaAla: 6.87 ± 0.562
0.608AlaCys: 0.608 ± 0.123
4.456AlaAsp: 4.456 ± 0.319
4.083AlaGlu: 4.083 ± 0.307
2.336AlaPhe: 2.336 ± 0.21
5.123AlaGly: 5.123 ± 0.446
1.237AlaHis: 1.237 ± 0.171
5.535AlaIle: 5.535 ± 0.32
4.044AlaLys: 4.044 ± 0.329
5.83AlaLeu: 5.83 ± 0.333
2.316AlaMet: 2.316 ± 0.216
3.494AlaAsn: 3.494 ± 0.277
2.984AlaPro: 2.984 ± 0.291
2.571AlaGln: 2.571 ± 0.245
3.258AlaArg: 3.258 ± 0.243
4.652AlaSer: 4.652 ± 0.301
4.848AlaThr: 4.848 ± 0.408
4.711AlaVal: 4.711 ± 0.32
0.981AlaTrp: 0.981 ± 0.142
2.218AlaTyr: 2.218 ± 0.199
0.0AlaXaa: 0.0 ± 0.0
Cys
0.628CysAla: 0.628 ± 0.138
0.118CysCys: 0.118 ± 0.046
0.903CysAsp: 0.903 ± 0.124
0.55CysGlu: 0.55 ± 0.096
0.275CysPhe: 0.275 ± 0.063
1.021CysGly: 1.021 ± 0.156
0.334CysHis: 0.334 ± 0.091
0.589CysIle: 0.589 ± 0.105
0.844CysLys: 0.844 ± 0.12
0.628CysLeu: 0.628 ± 0.105
0.294CysMet: 0.294 ± 0.071
0.766CysAsn: 0.766 ± 0.139
0.55CysPro: 0.55 ± 0.105
0.373CysGln: 0.373 ± 0.087
0.55CysArg: 0.55 ± 0.086
0.569CysSer: 0.569 ± 0.115
0.53CysThr: 0.53 ± 0.102
0.648CysVal: 0.648 ± 0.11
0.098CysTrp: 0.098 ± 0.051
0.491CysTyr: 0.491 ± 0.095
0.0CysXaa: 0.0 ± 0.0
Asp
4.201AspAla: 4.201 ± 0.404
0.726AspCys: 0.726 ± 0.116
3.082AspAsp: 3.082 ± 0.278
4.377AspGlu: 4.377 ± 0.357
2.395AspPhe: 2.395 ± 0.235
3.553AspGly: 3.553 ± 0.247
1.629AspHis: 1.629 ± 0.152
5.241AspIle: 5.241 ± 0.323
4.515AspLys: 4.515 ± 0.336
5.673AspLeu: 5.673 ± 0.393
1.845AspMet: 1.845 ± 0.173
3.396AspAsn: 3.396 ± 0.275
2.316AspPro: 2.316 ± 0.233
2.571AspGln: 2.571 ± 0.251
2.493AspArg: 2.493 ± 0.187
4.318AspSer: 4.318 ± 0.275
4.122AspThr: 4.122 ± 0.288
3.788AspVal: 3.788 ± 0.297
0.707AspTrp: 0.707 ± 0.121
3.062AspTyr: 3.062 ± 0.244
0.0AspXaa: 0.0 ± 0.0
Glu
4.907GluAla: 4.907 ± 0.297
0.805GluCys: 0.805 ± 0.132
3.18GluAsp: 3.18 ± 0.238
3.73GluGlu: 3.73 ± 0.362
2.905GluPhe: 2.905 ± 0.289
2.728GluGly: 2.728 ± 0.23
1.453GluHis: 1.453 ± 0.184
4.102GluIle: 4.102 ± 0.284
3.219GluLys: 3.219 ± 0.265
6.36GluLeu: 6.36 ± 0.44
1.668GluMet: 1.668 ± 0.194
2.552GluAsn: 2.552 ± 0.231
1.668GluPro: 1.668 ± 0.174
2.473GluGln: 2.473 ± 0.3
2.198GluArg: 2.198 ± 0.212
3.906GluSer: 3.906 ± 0.315
2.316GluThr: 2.316 ± 0.242
4.318GluVal: 4.318 ± 0.395
0.805GluTrp: 0.805 ± 0.124
3.042GluTyr: 3.042 ± 0.223
0.0GluXaa: 0.0 ± 0.0
Phe
2.336PheAla: 2.336 ± 0.211
0.569PheCys: 0.569 ± 0.103
2.984PheAsp: 2.984 ± 0.219
2.041PheGlu: 2.041 ± 0.211
1.06PhePhe: 1.06 ± 0.152
2.395PheGly: 2.395 ± 0.208
0.707PheHis: 0.707 ± 0.113
2.709PheIle: 2.709 ± 0.25
2.728PheLys: 2.728 ± 0.223
2.846PheLeu: 2.846 ± 0.231
1.217PheMet: 1.217 ± 0.135
2.964PheAsn: 2.964 ± 0.225
1.296PhePro: 1.296 ± 0.139
1.158PheGln: 1.158 ± 0.14
1.138PheArg: 1.138 ± 0.15
3.121PheSer: 3.121 ± 0.236
2.316PheThr: 2.316 ± 0.219
2.12PheVal: 2.12 ± 0.207
0.471PheTrp: 0.471 ± 0.102
1.178PheTyr: 1.178 ± 0.167
0.0PheXaa: 0.0 ± 0.0
Gly
3.985GlyAla: 3.985 ± 0.427
0.451GlyCys: 0.451 ± 0.087
4.044GlyAsp: 4.044 ± 0.258
2.709GlyGlu: 2.709 ± 0.248
2.454GlyPhe: 2.454 ± 0.226
4.122GlyGly: 4.122 ± 0.533
1.237GlyHis: 1.237 ± 0.149
4.947GlyIle: 4.947 ± 0.311
4.417GlyLys: 4.417 ± 0.287
4.456GlyLeu: 4.456 ± 0.263
1.767GlyMet: 1.767 ± 0.187
3.357GlyAsn: 3.357 ± 0.271
1.708GlyPro: 1.708 ± 0.182
2.1GlyGln: 2.1 ± 0.208
2.748GlyArg: 2.748 ± 0.239
3.572GlySer: 3.572 ± 0.364
4.122GlyThr: 4.122 ± 0.314
4.809GlyVal: 4.809 ± 0.286
0.942GlyTrp: 0.942 ± 0.142
2.434GlyTyr: 2.434 ± 0.211
0.0GlyXaa: 0.0 ± 0.0
His
1.551HisAla: 1.551 ± 0.201
0.236HisCys: 0.236 ± 0.063
1.354HisAsp: 1.354 ± 0.162
1.001HisGlu: 1.001 ± 0.156
0.844HisPhe: 0.844 ± 0.134
1.531HisGly: 1.531 ± 0.205
0.726HisHis: 0.726 ± 0.136
1.413HisIle: 1.413 ± 0.161
1.511HisLys: 1.511 ± 0.208
2.022HisLeu: 2.022 ± 0.208
0.589HisMet: 0.589 ± 0.124
1.296HisAsn: 1.296 ± 0.145
1.119HisPro: 1.119 ± 0.15
0.766HisGln: 0.766 ± 0.12
1.119HisArg: 1.119 ± 0.173
1.178HisSer: 1.178 ± 0.149
1.394HisThr: 1.394 ± 0.162
1.315HisVal: 1.315 ± 0.179
0.275HisTrp: 0.275 ± 0.07
1.08HisTyr: 1.08 ± 0.14
0.0HisXaa: 0.0 ± 0.0
Ile
5.182IleAla: 5.182 ± 0.338
0.687IleCys: 0.687 ± 0.123
5.261IleAsp: 5.261 ± 0.336
4.809IleGlu: 4.809 ± 0.294
2.14IlePhe: 2.14 ± 0.22
3.985IleGly: 3.985 ± 0.322
1.61IleHis: 1.61 ± 0.238
4.613IleIle: 4.613 ± 0.34
5.555IleLys: 5.555 ± 0.321
5.064IleLeu: 5.064 ± 0.315
1.197IleMet: 1.197 ± 0.16
5.025IleAsn: 5.025 ± 0.311
3.239IlePro: 3.239 ± 0.27
2.905IleGln: 2.905 ± 0.236
3.042IleArg: 3.042 ± 0.266
4.201IleSer: 4.201 ± 0.291
5.123IleThr: 5.123 ± 0.337
4.691IleVal: 4.691 ± 0.324
0.805IleTrp: 0.805 ± 0.114
2.12IleTyr: 2.12 ± 0.182
0.0IleXaa: 0.0 ± 0.0
Lys
4.475LysAla: 4.475 ± 0.295
0.785LysCys: 0.785 ± 0.129
4.632LysAsp: 4.632 ± 0.358
3.887LysGlu: 3.887 ± 0.385
2.905LysPhe: 2.905 ± 0.242
2.355LysGly: 2.355 ± 0.267
1.59LysHis: 1.59 ± 0.187
4.829LysIle: 4.829 ± 0.317
3.985LysLys: 3.985 ± 0.326
5.712LysLeu: 5.712 ± 0.334
2.257LysMet: 2.257 ± 0.216
3.376LysAsn: 3.376 ± 0.284
1.845LysPro: 1.845 ± 0.221
2.159LysGln: 2.159 ± 0.184
2.414LysArg: 2.414 ± 0.245
4.22LysSer: 4.22 ± 0.339
3.985LysThr: 3.985 ± 0.246
4.044LysVal: 4.044 ± 0.285
1.197LysTrp: 1.197 ± 0.163
4.102LysTyr: 4.102 ± 0.277
0.0LysXaa: 0.0 ± 0.0
Leu
6.164LeuAla: 6.164 ± 0.354
0.962LeuCys: 0.962 ± 0.136
6.222LeuAsp: 6.222 ± 0.352
4.299LeuGlu: 4.299 ± 0.314
2.807LeuPhe: 2.807 ± 0.235
5.241LeuGly: 5.241 ± 0.361
2.257LeuHis: 2.257 ± 0.251
5.221LeuIle: 5.221 ± 0.347
5.123LeuLys: 5.123 ± 0.424
6.733LeuLeu: 6.733 ± 0.398
2.218LeuMet: 2.218 ± 0.221
4.809LeuAsn: 4.809 ± 0.367
3.533LeuPro: 3.533 ± 0.266
3.121LeuGln: 3.121 ± 0.236
3.69LeuArg: 3.69 ± 0.303
5.849LeuSer: 5.849 ± 0.351
5.084LeuThr: 5.084 ± 0.39
5.81LeuVal: 5.81 ± 0.401
0.667LeuTrp: 0.667 ± 0.106
3.003LeuTyr: 3.003 ± 0.281
0.0LeuXaa: 0.0 ± 0.0
Met
1.767MetAla: 1.767 ± 0.18
0.275MetCys: 0.275 ± 0.072
1.472MetAsp: 1.472 ± 0.173
1.61MetGlu: 1.61 ± 0.193
1.138MetPhe: 1.138 ± 0.162
1.354MetGly: 1.354 ± 0.182
0.51MetHis: 0.51 ± 0.107
1.688MetIle: 1.688 ± 0.178
2.316MetLys: 2.316 ± 0.219
1.963MetLeu: 1.963 ± 0.213
0.805MetMet: 0.805 ± 0.193
1.61MetAsn: 1.61 ± 0.2
1.217MetPro: 1.217 ± 0.163
0.962MetGln: 0.962 ± 0.158
1.237MetArg: 1.237 ± 0.17
2.473MetSer: 2.473 ± 0.235
1.649MetThr: 1.649 ± 0.181
1.433MetVal: 1.433 ± 0.165
0.334MetTrp: 0.334 ± 0.089
1.021MetTyr: 1.021 ± 0.159
0.0MetXaa: 0.0 ± 0.0
Asn
3.749AsnAla: 3.749 ± 0.321
0.628AsnCys: 0.628 ± 0.118
2.571AsnAsp: 2.571 ± 0.196
3.357AsnGlu: 3.357 ± 0.287
1.511AsnPhe: 1.511 ± 0.181
3.357AsnGly: 3.357 ± 0.279
1.06AsnHis: 1.06 ± 0.133
4.122AsnIle: 4.122 ± 0.283
4.044AsnLys: 4.044 ± 0.258
4.77AsnLeu: 4.77 ± 0.366
1.374AsnMet: 1.374 ± 0.208
3.042AsnAsn: 3.042 ± 0.323
2.552AsnPro: 2.552 ± 0.246
1.865AsnGln: 1.865 ± 0.208
2.689AsnArg: 2.689 ± 0.219
3.69AsnSer: 3.69 ± 0.335
4.554AsnThr: 4.554 ± 0.297
3.474AsnVal: 3.474 ± 0.244
0.628AsnTrp: 0.628 ± 0.112
2.513AsnTyr: 2.513 ± 0.233
0.0AsnXaa: 0.0 ± 0.0
Pro
2.67ProAla: 2.67 ± 0.233
0.196ProCys: 0.196 ± 0.059
2.297ProAsp: 2.297 ± 0.218
2.689ProGlu: 2.689 ± 0.261
1.453ProPhe: 1.453 ± 0.187
2.513ProGly: 2.513 ± 0.252
0.864ProHis: 0.864 ± 0.135
2.552ProIle: 2.552 ± 0.227
2.1ProLys: 2.1 ± 0.23
2.768ProLeu: 2.768 ± 0.236
0.864ProMet: 0.864 ± 0.134
1.943ProAsn: 1.943 ± 0.203
1.276ProPro: 1.276 ± 0.17
1.551ProGln: 1.551 ± 0.184
1.276ProArg: 1.276 ± 0.17
2.591ProSer: 2.591 ± 0.263
2.709ProThr: 2.709 ± 0.255
3.239ProVal: 3.239 ± 0.3
0.491ProTrp: 0.491 ± 0.083
2.022ProTyr: 2.022 ± 0.213
0.0ProXaa: 0.0 ± 0.0
Gln
3.16GlnAla: 3.16 ± 0.302
0.451GlnCys: 0.451 ± 0.107
2.355GlnAsp: 2.355 ± 0.164
2.297GlnGlu: 2.297 ± 0.227
1.825GlnPhe: 1.825 ± 0.191
1.865GlnGly: 1.865 ± 0.2
0.687GlnHis: 0.687 ± 0.104
2.63GlnIle: 2.63 ± 0.253
1.884GlnLys: 1.884 ± 0.198
3.2GlnLeu: 3.2 ± 0.285
1.315GlnMet: 1.315 ± 0.184
1.767GlnAsn: 1.767 ± 0.179
1.021GlnPro: 1.021 ± 0.17
1.767GlnGln: 1.767 ± 0.246
1.531GlnArg: 1.531 ± 0.16
3.219GlnSer: 3.219 ± 0.269
1.629GlnThr: 1.629 ± 0.146
2.866GlnVal: 2.866 ± 0.22
0.51GlnTrp: 0.51 ± 0.104
1.727GlnTyr: 1.727 ± 0.252
0.0GlnXaa: 0.0 ± 0.0
Arg
3.317ArgAla: 3.317 ± 0.275
0.314ArgCys: 0.314 ± 0.076
2.611ArgAsp: 2.611 ± 0.2
2.944ArgGlu: 2.944 ± 0.304
1.865ArgPhe: 1.865 ± 0.223
2.513ArgGly: 2.513 ± 0.211
0.864ArgHis: 0.864 ± 0.108
3.533ArgIle: 3.533 ± 0.266
2.434ArgLys: 2.434 ± 0.251
3.474ArgLeu: 3.474 ± 0.267
1.08ArgMet: 1.08 ± 0.141
2.1ArgAsn: 2.1 ± 0.211
1.335ArgPro: 1.335 ± 0.182
1.453ArgGln: 1.453 ± 0.181
2.395ArgArg: 2.395 ± 0.273
2.787ArgSer: 2.787 ± 0.229
2.297ArgThr: 2.297 ± 0.257
3.337ArgVal: 3.337 ± 0.217
0.628ArgTrp: 0.628 ± 0.103
1.983ArgTyr: 1.983 ± 0.199
0.0ArgXaa: 0.0 ± 0.0
Ser
4.888SerAla: 4.888 ± 0.394
0.766SerCys: 0.766 ± 0.113
4.142SerAsp: 4.142 ± 0.289
3.455SerGlu: 3.455 ± 0.261
2.846SerPhe: 2.846 ± 0.217
4.574SerGly: 4.574 ± 0.387
1.767SerHis: 1.767 ± 0.171
4.907SerIle: 4.907 ± 0.356
3.985SerLys: 3.985 ± 0.305
5.457SerLeu: 5.457 ± 0.4
1.884SerMet: 1.884 ± 0.178
4.044SerAsn: 4.044 ± 0.386
2.63SerPro: 2.63 ± 0.226
2.355SerGln: 2.355 ± 0.21
2.984SerArg: 2.984 ± 0.282
4.731SerSer: 4.731 ± 0.412
4.436SerThr: 4.436 ± 0.272
4.75SerVal: 4.75 ± 0.309
0.707SerTrp: 0.707 ± 0.119
2.513SerTyr: 2.513 ± 0.212
0.0SerXaa: 0.0 ± 0.0
Thr
4.534ThrAla: 4.534 ± 0.442
0.569ThrCys: 0.569 ± 0.113
3.631ThrAsp: 3.631 ± 0.26
3.533ThrGlu: 3.533 ± 0.282
2.297ThrPhe: 2.297 ± 0.201
4.299ThrGly: 4.299 ± 0.292
1.256ThrHis: 1.256 ± 0.136
4.907ThrIle: 4.907 ± 0.295
4.279ThrLys: 4.279 ± 0.319
5.359ThrLeu: 5.359 ± 0.342
1.315ThrMet: 1.315 ± 0.163
3.258ThrAsn: 3.258 ± 0.268
3.121ThrPro: 3.121 ± 0.243
2.081ThrGln: 2.081 ± 0.222
2.728ThrArg: 2.728 ± 0.261
4.279ThrSer: 4.279 ± 0.362
4.397ThrThr: 4.397 ± 0.351
4.888ThrVal: 4.888 ± 0.37
0.687ThrTrp: 0.687 ± 0.123
2.513ThrTyr: 2.513 ± 0.231
0.0ThrXaa: 0.0 ± 0.0
Val
4.652ValAla: 4.652 ± 0.28
0.707ValCys: 0.707 ± 0.11
4.966ValAsp: 4.966 ± 0.293
3.651ValGlu: 3.651 ± 0.254
2.513ValPhe: 2.513 ± 0.218
4.456ValGly: 4.456 ± 0.391
1.276ValHis: 1.276 ± 0.151
4.358ValIle: 4.358 ± 0.292
4.181ValLys: 4.181 ± 0.283
5.712ValLeu: 5.712 ± 0.313
1.668ValMet: 1.668 ± 0.161
3.651ValAsn: 3.651 ± 0.262
2.866ValPro: 2.866 ± 0.241
3.239ValGln: 3.239 ± 0.273
3.18ValArg: 3.18 ± 0.247
4.475ValSer: 4.475 ± 0.36
4.829ValThr: 4.829 ± 0.341
5.005ValVal: 5.005 ± 0.313
0.707ValTrp: 0.707 ± 0.109
2.297ValTyr: 2.297 ± 0.23
0.0ValXaa: 0.0 ± 0.0
Trp
0.648TrpAla: 0.648 ± 0.106
0.255TrpCys: 0.255 ± 0.06
0.883TrpAsp: 0.883 ± 0.144
0.707TrpGlu: 0.707 ± 0.117
0.53TrpPhe: 0.53 ± 0.109
0.589TrpGly: 0.589 ± 0.11
0.294TrpHis: 0.294 ± 0.084
0.608TrpIle: 0.608 ± 0.106
0.844TrpLys: 0.844 ± 0.156
0.903TrpLeu: 0.903 ± 0.119
0.471TrpMet: 0.471 ± 0.114
0.726TrpAsn: 0.726 ± 0.133
0.294TrpPro: 0.294 ± 0.071
0.471TrpGln: 0.471 ± 0.099
0.589TrpArg: 0.589 ± 0.117
1.217TrpSer: 1.217 ± 0.158
0.766TrpThr: 0.766 ± 0.133
0.766TrpVal: 0.766 ± 0.131
0.118TrpTrp: 0.118 ± 0.048
0.608TrpTyr: 0.608 ± 0.114
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.473TyrAla: 2.473 ± 0.199
0.648TyrCys: 0.648 ± 0.102
2.964TyrAsp: 2.964 ± 0.222
2.611TyrGlu: 2.611 ± 0.231
1.256TyrPhe: 1.256 ± 0.181
2.807TyrGly: 2.807 ± 0.199
0.962TyrHis: 0.962 ± 0.146
2.748TyrIle: 2.748 ± 0.217
2.748TyrLys: 2.748 ± 0.272
3.887TyrLeu: 3.887 ± 0.294
0.667TyrMet: 0.667 ± 0.147
2.375TyrAsn: 2.375 ± 0.211
1.492TyrPro: 1.492 ± 0.172
1.767TyrGln: 1.767 ± 0.201
2.022TyrArg: 2.022 ± 0.185
2.768TyrSer: 2.768 ± 0.267
2.827TyrThr: 2.827 ± 0.236
2.375TyrVal: 2.375 ± 0.224
0.53TyrTrp: 0.53 ± 0.095
2.1TyrTyr: 2.1 ± 0.204
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 264 proteins (50946 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski