Amino acid dipepetide frequency for Edwardsiella phage IW-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.871AlaAla: 9.871 ± 1.22
0.683AlaCys: 0.683 ± 0.244
5.923AlaAsp: 5.923 ± 0.958
5.771AlaGlu: 5.771 ± 0.572
3.189AlaPhe: 3.189 ± 0.506
7.137AlaGly: 7.137 ± 0.914
1.367AlaHis: 1.367 ± 0.283
4.328AlaIle: 4.328 ± 0.582
5.391AlaLys: 5.391 ± 0.85
7.669AlaLeu: 7.669 ± 0.821
4.1AlaMet: 4.1 ± 0.419
4.48AlaAsn: 4.48 ± 0.518
3.189AlaPro: 3.189 ± 0.562
4.024AlaGln: 4.024 ± 0.576
5.239AlaArg: 5.239 ± 0.816
6.302AlaSer: 6.302 ± 1.044
5.315AlaThr: 5.315 ± 0.745
6.302AlaVal: 6.302 ± 0.64
1.746AlaTrp: 1.746 ± 0.388
3.797AlaTyr: 3.797 ± 0.537
0.0AlaXaa: 0.0 ± 0.0
Cys
1.443CysAla: 1.443 ± 0.364
0.152CysCys: 0.152 ± 0.109
0.683CysAsp: 0.683 ± 0.205
0.532CysGlu: 0.532 ± 0.191
0.38CysPhe: 0.38 ± 0.186
0.456CysGly: 0.456 ± 0.219
0.38CysHis: 0.38 ± 0.202
0.683CysIle: 0.683 ± 0.238
1.063CysLys: 1.063 ± 0.3
0.911CysLeu: 0.911 ± 0.288
0.152CysMet: 0.152 ± 0.1
0.607CysAsn: 0.607 ± 0.212
0.228CysPro: 0.228 ± 0.122
0.38CysGln: 0.38 ± 0.158
0.076CysArg: 0.076 ± 0.092
0.607CysSer: 0.607 ± 0.248
0.456CysThr: 0.456 ± 0.163
0.911CysVal: 0.911 ± 0.277
0.152CysTrp: 0.152 ± 0.107
0.38CysTyr: 0.38 ± 0.179
0.0CysXaa: 0.0 ± 0.0
Asp
6.226AspAla: 6.226 ± 0.626
0.607AspCys: 0.607 ± 0.218
4.176AspAsp: 4.176 ± 0.581
3.645AspGlu: 3.645 ± 0.672
3.037AspPhe: 3.037 ± 0.548
4.252AspGly: 4.252 ± 0.565
0.38AspHis: 0.38 ± 0.145
3.948AspIle: 3.948 ± 0.572
4.176AspLys: 4.176 ± 0.582
5.011AspLeu: 5.011 ± 0.622
1.974AspMet: 1.974 ± 0.567
2.354AspAsn: 2.354 ± 0.407
3.037AspPro: 3.037 ± 0.462
1.291AspGln: 1.291 ± 0.319
2.126AspArg: 2.126 ± 0.409
3.872AspSer: 3.872 ± 0.473
4.024AspThr: 4.024 ± 0.533
5.163AspVal: 5.163 ± 0.559
0.911AspTrp: 0.911 ± 0.237
2.43AspTyr: 2.43 ± 0.401
0.0AspXaa: 0.0 ± 0.0
Glu
6.226GluAla: 6.226 ± 0.884
0.759GluCys: 0.759 ± 0.297
3.493GluAsp: 3.493 ± 0.358
3.645GluGlu: 3.645 ± 0.465
2.582GluPhe: 2.582 ± 0.339
3.797GluGly: 3.797 ± 0.505
1.519GluHis: 1.519 ± 0.374
1.822GluIle: 1.822 ± 0.362
2.43GluLys: 2.43 ± 0.451
8.428GluLeu: 8.428 ± 0.79
1.367GluMet: 1.367 ± 0.326
1.367GluAsn: 1.367 ± 0.271
1.595GluPro: 1.595 ± 0.362
2.885GluGln: 2.885 ± 0.541
2.658GluArg: 2.658 ± 0.506
2.43GluSer: 2.43 ± 0.33
3.341GluThr: 3.341 ± 0.519
5.011GluVal: 5.011 ± 0.615
0.759GluTrp: 0.759 ± 0.202
2.885GluTyr: 2.885 ± 0.582
0.0GluXaa: 0.0 ± 0.0
Phe
2.202PheAla: 2.202 ± 0.38
0.532PheCys: 0.532 ± 0.185
3.189PheAsp: 3.189 ± 0.434
2.354PheGlu: 2.354 ± 0.416
0.835PhePhe: 0.835 ± 0.199
3.493PheGly: 3.493 ± 0.658
0.532PheHis: 0.532 ± 0.185
2.126PheIle: 2.126 ± 0.481
1.746PheLys: 1.746 ± 0.386
3.037PheLeu: 3.037 ± 0.581
0.38PheMet: 0.38 ± 0.182
2.126PheAsn: 2.126 ± 0.413
0.835PhePro: 0.835 ± 0.272
0.835PheGln: 0.835 ± 0.171
1.974PheArg: 1.974 ± 0.38
2.126PheSer: 2.126 ± 0.402
2.43PheThr: 2.43 ± 0.595
1.898PheVal: 1.898 ± 0.456
0.759PheTrp: 0.759 ± 0.231
1.139PheTyr: 1.139 ± 0.268
0.0PheXaa: 0.0 ± 0.0
Gly
6.226GlyAla: 6.226 ± 0.855
0.911GlyCys: 0.911 ± 0.273
3.872GlyAsp: 3.872 ± 0.573
5.087GlyGlu: 5.087 ± 0.677
2.885GlyPhe: 2.885 ± 0.388
5.087GlyGly: 5.087 ± 0.802
0.987GlyHis: 0.987 ± 0.29
4.252GlyIle: 4.252 ± 0.512
5.087GlyLys: 5.087 ± 0.653
6.226GlyLeu: 6.226 ± 0.751
1.367GlyMet: 1.367 ± 0.234
4.252GlyAsn: 4.252 ± 0.537
1.367GlyPro: 1.367 ± 0.334
2.582GlyGln: 2.582 ± 0.505
3.113GlyArg: 3.113 ± 0.383
5.239GlySer: 5.239 ± 0.726
4.784GlyThr: 4.784 ± 0.937
5.619GlyVal: 5.619 ± 0.75
0.987GlyTrp: 0.987 ± 0.29
2.961GlyTyr: 2.961 ± 0.446
0.0GlyXaa: 0.0 ± 0.0
His
1.367HisAla: 1.367 ± 0.331
0.076HisCys: 0.076 ± 0.073
0.683HisAsp: 0.683 ± 0.224
0.532HisGlu: 0.532 ± 0.19
0.911HisPhe: 0.911 ± 0.302
0.987HisGly: 0.987 ± 0.256
0.228HisHis: 0.228 ± 0.128
1.139HisIle: 1.139 ± 0.334
1.443HisLys: 1.443 ± 0.295
0.987HisLeu: 0.987 ± 0.28
0.38HisMet: 0.38 ± 0.167
1.367HisAsn: 1.367 ± 0.319
0.456HisPro: 0.456 ± 0.218
0.607HisGln: 0.607 ± 0.257
0.607HisArg: 0.607 ± 0.182
1.139HisSer: 1.139 ± 0.239
0.835HisThr: 0.835 ± 0.25
1.063HisVal: 1.063 ± 0.304
0.0HisTrp: 0.0 ± 0.0
1.367HisTyr: 1.367 ± 0.399
0.0HisXaa: 0.0 ± 0.0
Ile
4.404IleAla: 4.404 ± 0.479
0.532IleCys: 0.532 ± 0.2
3.948IleAsp: 3.948 ± 0.463
3.417IleGlu: 3.417 ± 0.541
1.367IlePhe: 1.367 ± 0.338
2.885IleGly: 2.885 ± 0.581
0.456IleHis: 0.456 ± 0.182
2.43IleIle: 2.43 ± 0.461
3.341IleLys: 3.341 ± 0.537
3.341IleLeu: 3.341 ± 0.721
1.443IleMet: 1.443 ± 0.342
2.658IleAsn: 2.658 ± 0.541
1.367IlePro: 1.367 ± 0.277
1.67IleGln: 1.67 ± 0.41
1.974IleArg: 1.974 ± 0.288
2.809IleSer: 2.809 ± 0.415
3.189IleThr: 3.189 ± 0.429
3.948IleVal: 3.948 ± 0.436
0.38IleTrp: 0.38 ± 0.167
1.519IleTyr: 1.519 ± 0.26
0.0IleXaa: 0.0 ± 0.0
Lys
6.986LysAla: 6.986 ± 0.767
0.607LysCys: 0.607 ± 0.2
3.417LysAsp: 3.417 ± 0.427
3.037LysGlu: 3.037 ± 0.606
1.898LysPhe: 1.898 ± 0.399
4.176LysGly: 4.176 ± 0.83
1.519LysHis: 1.519 ± 0.388
2.05LysIle: 2.05 ± 0.378
2.582LysLys: 2.582 ± 0.466
8.2LysLeu: 8.2 ± 0.78
1.215LysMet: 1.215 ± 0.319
1.67LysAsn: 1.67 ± 0.404
2.658LysPro: 2.658 ± 0.459
2.658LysGln: 2.658 ± 0.433
3.417LysArg: 3.417 ± 0.581
3.037LysSer: 3.037 ± 0.316
3.417LysThr: 3.417 ± 0.51
4.48LysVal: 4.48 ± 0.542
0.759LysTrp: 0.759 ± 0.231
2.658LysTyr: 2.658 ± 0.399
0.0LysXaa: 0.0 ± 0.0
Leu
9.871LeuAla: 9.871 ± 0.801
0.607LeuCys: 0.607 ± 0.187
6.53LeuAsp: 6.53 ± 0.665
5.391LeuGlu: 5.391 ± 0.607
1.595LeuPhe: 1.595 ± 0.271
5.695LeuGly: 5.695 ± 0.798
2.126LeuHis: 2.126 ± 0.438
3.037LeuIle: 3.037 ± 0.546
4.86LeuLys: 4.86 ± 0.675
7.593LeuLeu: 7.593 ± 0.7
2.278LeuMet: 2.278 ± 0.303
4.024LeuAsn: 4.024 ± 0.478
4.1LeuPro: 4.1 ± 0.617
4.86LeuGln: 4.86 ± 0.658
4.404LeuArg: 4.404 ± 0.509
5.847LeuSer: 5.847 ± 0.685
5.543LeuThr: 5.543 ± 0.579
6.986LeuVal: 6.986 ± 0.713
0.987LeuTrp: 0.987 ± 0.254
3.569LeuTyr: 3.569 ± 0.496
0.0LeuXaa: 0.0 ± 0.0
Met
2.43MetAla: 2.43 ± 0.503
0.228MetCys: 0.228 ± 0.124
1.67MetAsp: 1.67 ± 0.455
0.835MetGlu: 0.835 ± 0.235
0.911MetPhe: 0.911 ± 0.26
0.911MetGly: 0.911 ± 0.216
0.532MetHis: 0.532 ± 0.178
0.987MetIle: 0.987 ± 0.281
1.215MetLys: 1.215 ± 0.308
2.733MetLeu: 2.733 ± 0.414
0.532MetMet: 0.532 ± 0.291
1.139MetAsn: 1.139 ± 0.32
1.367MetPro: 1.367 ± 0.363
1.367MetGln: 1.367 ± 0.379
1.822MetArg: 1.822 ± 0.411
2.43MetSer: 2.43 ± 0.53
2.43MetThr: 2.43 ± 0.32
0.911MetVal: 0.911 ± 0.276
0.304MetTrp: 0.304 ± 0.136
0.911MetTyr: 0.911 ± 0.265
0.0MetXaa: 0.0 ± 0.0
Asn
3.948AsnAla: 3.948 ± 0.599
0.987AsnCys: 0.987 ± 0.357
2.05AsnAsp: 2.05 ± 0.342
2.809AsnGlu: 2.809 ± 0.49
1.139AsnPhe: 1.139 ± 0.415
4.48AsnGly: 4.48 ± 0.764
0.532AsnHis: 0.532 ± 0.227
2.354AsnIle: 2.354 ± 0.374
2.733AsnLys: 2.733 ± 0.466
4.1AsnLeu: 4.1 ± 0.479
1.367AsnMet: 1.367 ± 0.304
1.746AsnAsn: 1.746 ± 0.384
2.05AsnPro: 2.05 ± 0.342
1.898AsnGln: 1.898 ± 0.349
1.291AsnArg: 1.291 ± 0.473
2.885AsnSer: 2.885 ± 0.487
2.658AsnThr: 2.658 ± 0.359
3.417AsnVal: 3.417 ± 0.496
0.456AsnTrp: 0.456 ± 0.162
1.822AsnTyr: 1.822 ± 0.397
0.0AsnXaa: 0.0 ± 0.0
Pro
2.961ProAla: 2.961 ± 0.457
0.228ProCys: 0.228 ± 0.108
2.506ProAsp: 2.506 ± 0.546
2.733ProGlu: 2.733 ± 0.501
0.683ProPhe: 0.683 ± 0.273
2.582ProGly: 2.582 ± 0.461
0.304ProHis: 0.304 ± 0.19
1.519ProIle: 1.519 ± 0.36
2.809ProLys: 2.809 ± 0.477
1.974ProLeu: 1.974 ± 0.503
1.291ProMet: 1.291 ± 0.378
1.898ProAsn: 1.898 ± 0.373
0.911ProPro: 0.911 ± 0.292
1.746ProGln: 1.746 ± 0.372
1.443ProArg: 1.443 ± 0.255
3.417ProSer: 3.417 ± 0.695
2.961ProThr: 2.961 ± 0.552
3.569ProVal: 3.569 ± 0.527
0.38ProTrp: 0.38 ± 0.151
0.911ProTyr: 0.911 ± 0.31
0.0ProXaa: 0.0 ± 0.0
Gln
5.087GlnAla: 5.087 ± 1.035
0.456GlnCys: 0.456 ± 0.195
1.898GlnAsp: 1.898 ± 0.354
2.43GlnGlu: 2.43 ± 0.361
1.519GlnPhe: 1.519 ± 0.394
2.354GlnGly: 2.354 ± 0.479
0.683GlnHis: 0.683 ± 0.216
1.519GlnIle: 1.519 ± 0.351
1.367GlnLys: 1.367 ± 0.251
4.404GlnLeu: 4.404 ± 0.587
0.987GlnMet: 0.987 ± 0.187
1.595GlnAsn: 1.595 ± 0.31
1.746GlnPro: 1.746 ± 0.263
3.872GlnGln: 3.872 ± 0.768
2.961GlnArg: 2.961 ± 0.573
2.05GlnSer: 2.05 ± 0.393
1.974GlnThr: 1.974 ± 0.346
3.037GlnVal: 3.037 ± 0.499
0.759GlnTrp: 0.759 ± 0.235
1.822GlnTyr: 1.822 ± 0.453
0.0GlnXaa: 0.0 ± 0.0
Arg
4.176ArgAla: 4.176 ± 0.569
0.38ArgCys: 0.38 ± 0.153
2.506ArgAsp: 2.506 ± 0.489
3.341ArgGlu: 3.341 ± 0.494
1.67ArgPhe: 1.67 ± 0.332
3.645ArgGly: 3.645 ± 0.437
0.835ArgHis: 0.835 ± 0.286
2.961ArgIle: 2.961 ± 0.378
2.733ArgLys: 2.733 ± 0.548
3.417ArgLeu: 3.417 ± 0.477
0.911ArgMet: 0.911 ± 0.297
2.126ArgAsn: 2.126 ± 0.456
1.822ArgPro: 1.822 ± 0.405
2.658ArgGln: 2.658 ± 0.446
1.974ArgArg: 1.974 ± 0.377
2.582ArgSer: 2.582 ± 0.487
2.961ArgThr: 2.961 ± 0.501
4.556ArgVal: 4.556 ± 0.595
0.835ArgTrp: 0.835 ± 0.306
1.746ArgTyr: 1.746 ± 0.238
0.0ArgXaa: 0.0 ± 0.0
Ser
5.695SerAla: 5.695 ± 0.749
0.532SerCys: 0.532 ± 0.205
4.86SerAsp: 4.86 ± 0.482
3.417SerGlu: 3.417 ± 0.508
3.341SerPhe: 3.341 ± 0.568
6.378SerGly: 6.378 ± 0.77
0.759SerHis: 0.759 ± 0.236
2.733SerIle: 2.733 ± 0.392
4.024SerLys: 4.024 ± 0.518
4.935SerLeu: 4.935 ± 0.64
1.139SerMet: 1.139 ± 0.282
2.126SerAsn: 2.126 ± 0.41
2.05SerPro: 2.05 ± 0.397
2.202SerGln: 2.202 ± 0.36
2.961SerArg: 2.961 ± 0.583
4.632SerSer: 4.632 ± 0.722
4.024SerThr: 4.024 ± 0.55
3.569SerVal: 3.569 ± 0.585
0.683SerTrp: 0.683 ± 0.185
2.506SerTyr: 2.506 ± 0.461
0.0SerXaa: 0.0 ± 0.0
Thr
5.619ThrAla: 5.619 ± 0.741
0.304ThrCys: 0.304 ± 0.146
3.797ThrAsp: 3.797 ± 0.374
2.658ThrGlu: 2.658 ± 0.524
2.733ThrPhe: 2.733 ± 0.539
6.682ThrGly: 6.682 ± 0.997
0.759ThrHis: 0.759 ± 0.193
3.797ThrIle: 3.797 ± 0.476
4.935ThrLys: 4.935 ± 0.516
5.087ThrLeu: 5.087 ± 0.666
1.519ThrMet: 1.519 ± 0.436
2.202ThrAsn: 2.202 ± 0.333
2.733ThrPro: 2.733 ± 0.536
2.354ThrGln: 2.354 ± 0.401
3.189ThrArg: 3.189 ± 0.469
3.037ThrSer: 3.037 ± 0.605
4.024ThrThr: 4.024 ± 0.532
4.1ThrVal: 4.1 ± 0.708
0.911ThrTrp: 0.911 ± 0.287
2.05ThrTyr: 2.05 ± 0.391
0.0ThrXaa: 0.0 ± 0.0
Val
7.137ValAla: 7.137 ± 0.736
1.139ValCys: 1.139 ± 0.339
4.176ValAsp: 4.176 ± 0.54
4.556ValGlu: 4.556 ± 0.463
2.506ValPhe: 2.506 ± 0.479
4.328ValGly: 4.328 ± 0.585
1.291ValHis: 1.291 ± 0.259
3.189ValIle: 3.189 ± 0.52
4.632ValLys: 4.632 ± 0.399
6.91ValLeu: 6.91 ± 0.735
2.05ValMet: 2.05 ± 0.46
3.569ValAsn: 3.569 ± 0.566
3.797ValPro: 3.797 ± 0.616
3.037ValGln: 3.037 ± 0.575
3.265ValArg: 3.265 ± 0.477
5.391ValSer: 5.391 ± 0.919
4.632ValThr: 4.632 ± 0.618
5.998ValVal: 5.998 ± 0.979
0.683ValTrp: 0.683 ± 0.249
3.037ValTyr: 3.037 ± 0.678
0.0ValXaa: 0.0 ± 0.0
Trp
0.683TrpAla: 0.683 ± 0.279
0.228TrpCys: 0.228 ± 0.119
0.987TrpAsp: 0.987 ± 0.325
0.683TrpGlu: 0.683 ± 0.196
0.38TrpPhe: 0.38 ± 0.163
0.683TrpGly: 0.683 ± 0.251
0.304TrpHis: 0.304 ± 0.142
0.304TrpIle: 0.304 ± 0.148
0.683TrpLys: 0.683 ± 0.235
1.67TrpLeu: 1.67 ± 0.375
0.304TrpMet: 0.304 ± 0.15
0.911TrpAsn: 0.911 ± 0.316
0.607TrpPro: 0.607 ± 0.201
0.152TrpGln: 0.152 ± 0.098
0.911TrpArg: 0.911 ± 0.276
0.38TrpSer: 0.38 ± 0.183
0.911TrpThr: 0.911 ± 0.29
1.595TrpVal: 1.595 ± 0.381
0.076TrpTrp: 0.076 ± 0.069
0.532TrpTyr: 0.532 ± 0.231
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.265TyrAla: 3.265 ± 0.419
0.683TyrCys: 0.683 ± 0.245
2.278TyrAsp: 2.278 ± 0.41
2.126TyrGlu: 2.126 ± 0.435
1.063TyrPhe: 1.063 ± 0.3
2.885TyrGly: 2.885 ± 0.421
0.683TyrHis: 0.683 ± 0.203
1.898TyrIle: 1.898 ± 0.364
3.189TyrLys: 3.189 ± 0.366
3.265TyrLeu: 3.265 ± 0.465
0.835TyrMet: 0.835 ± 0.255
2.506TyrAsn: 2.506 ± 0.423
0.911TyrPro: 0.911 ± 0.325
1.443TyrGln: 1.443 ± 0.299
2.43TyrArg: 2.43 ± 0.392
2.354TyrSer: 2.354 ± 0.459
2.582TyrThr: 2.582 ± 0.373
3.189TyrVal: 3.189 ± 0.523
0.456TyrTrp: 0.456 ± 0.162
1.898TyrTyr: 1.898 ± 0.424
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (13171 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski