Amino acid dipepetide frequency for Rhodobacteraceae bacterium EhC02

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.179AlaAla: 18.179 ± 0.18	1.125AlaCys: 1.125 ± 0.035	7.461AlaAsp: 7.461 ± 0.096	7.769AlaGlu: 7.769 ± 0.102	4.322AlaPhe: 4.322 ± 0.065	11.314AlaGly: 11.314 ± 0.143	2.605AlaHis: 2.605 ± 0.048	6.013AlaIle: 6.013 ± 0.072	3.242AlaLys: 3.242 ± 0.07	14.544AlaLeu: 14.544 ± 0.127	4.233AlaMet: 4.233 ± 0.061	2.7AlaAsn: 2.7 ± 0.058	6.155AlaPro: 6.155 ± 0.092	5.03AlaGln: 5.03 ± 0.068	9.85AlaArg: 9.85 ± 0.114	5.621AlaSer: 5.621 ± 0.075	6.211AlaThr: 6.211 ± 0.075	8.628AlaVal: 8.628 ± 0.1	1.484AlaTrp: 1.484 ± 0.038	2.464AlaTyr: 2.464 ± 0.043	0.001AlaXaa: 0.001 ± 0.001
Cys	1.082CysAla: 1.082 ± 0.031	0.113CysCys: 0.113 ± 0.01	0.574CysAsp: 0.574 ± 0.021	0.389CysGlu: 0.389 ± 0.018	0.337CysPhe: 0.337 ± 0.016	0.922CysGly: 0.922 ± 0.031	0.27CysHis: 0.27 ± 0.016	0.43CysIle: 0.43 ± 0.018	0.216CysLys: 0.216 ± 0.013	0.919CysLeu: 0.919 ± 0.032	0.194CysMet: 0.194 ± 0.013	0.225CysAsn: 0.225 ± 0.014	0.511CysPro: 0.511 ± 0.026	0.239CysGln: 0.239 ± 0.014	0.523CysArg: 0.523 ± 0.021	0.405CysSer: 0.405 ± 0.021	0.494CysThr: 0.494 ± 0.022	0.593CysVal: 0.593 ± 0.026	0.108CysTrp: 0.108 ± 0.011	0.202CysTyr: 0.202 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	7.468AspAla: 7.468 ± 0.092	0.545AspCys: 0.545 ± 0.023	3.276AspAsp: 3.276 ± 0.077	3.112AspGlu: 3.112 ± 0.061	2.154AspPhe: 2.154 ± 0.039	5.375AspGly: 5.375 ± 0.088	1.526AspHis: 1.526 ± 0.044	3.101AspIle: 3.101 ± 0.052	1.627AspLys: 1.627 ± 0.04	6.852AspLeu: 6.852 ± 0.087	1.922AspMet: 1.922 ± 0.044	1.215AspAsn: 1.215 ± 0.037	3.847AspPro: 3.847 ± 0.064	2.07AspGln: 2.07 ± 0.048	4.706AspArg: 4.706 ± 0.074	2.276AspSer: 2.276 ± 0.041	3.066AspThr: 3.066 ± 0.073	4.168AspVal: 4.168 ± 0.07	1.202AspTrp: 1.202 ± 0.032	1.487AspTyr: 1.487 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	7.892GluAla: 7.892 ± 0.089	0.309GluCys: 0.309 ± 0.018	3.112GluAsp: 3.112 ± 0.052	2.818GluGlu: 2.818 ± 0.065	1.578GluPhe: 1.578 ± 0.045	4.8GluGly: 4.8 ± 0.066	0.944GluHis: 0.944 ± 0.032	3.136GluIle: 3.136 ± 0.048	1.814GluLys: 1.814 ± 0.042	4.518GluLeu: 4.518 ± 0.073	1.778GluMet: 1.778 ± 0.045	1.502GluAsn: 1.502 ± 0.038	2.204GluPro: 2.204 ± 0.051	1.797GluGln: 1.797 ± 0.042	3.856GluArg: 3.856 ± 0.072	1.807GluSer: 1.807 ± 0.037	3.242GluThr: 3.242 ± 0.055	4.199GluVal: 4.199 ± 0.059	0.69GluTrp: 0.69 ± 0.023	0.956GluTyr: 0.956 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	4.444PheAla: 4.444 ± 0.066	0.418PheCys: 0.418 ± 0.017	2.838PheAsp: 2.838 ± 0.042	1.94PheGlu: 1.94 ± 0.04	1.404PhePhe: 1.404 ± 0.04	3.693PheGly: 3.693 ± 0.065	0.739PheHis: 0.739 ± 0.026	1.599PheIle: 1.599 ± 0.036	0.871PheLys: 0.871 ± 0.025	3.359PheLeu: 3.359 ± 0.06	0.899PheMet: 0.899 ± 0.031	1.036PheAsn: 1.036 ± 0.027	1.548PhePro: 1.548 ± 0.035	1.056PheGln: 1.056 ± 0.027	2.229PheArg: 2.229 ± 0.043	1.95PheSer: 1.95 ± 0.042	2.012PheThr: 2.012 ± 0.042	2.591PheVal: 2.591 ± 0.053	0.574PheTrp: 0.574 ± 0.025	0.911PheTyr: 0.911 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	10.42GlyAla: 10.42 ± 0.122	0.899GlyCys: 0.899 ± 0.025	4.758GlyAsp: 4.758 ± 0.101	4.066GlyGlu: 4.066 ± 0.064	3.74GlyPhe: 3.74 ± 0.054	7.66GlyGly: 7.66 ± 0.129	2.112GlyHis: 2.112 ± 0.042	4.662GlyIle: 4.662 ± 0.07	2.98GlyLys: 2.98 ± 0.052	9.708GlyLeu: 9.708 ± 0.115	2.888GlyMet: 2.888 ± 0.047	2.17GlyAsn: 2.17 ± 0.07	3.799GlyPro: 3.799 ± 0.063	3.7GlyGln: 3.7 ± 0.062	6.031GlyArg: 6.031 ± 0.071	4.107GlySer: 4.107 ± 0.068	4.705GlyThr: 4.705 ± 0.083	6.443GlyVal: 6.443 ± 0.082	1.524GlyTrp: 1.524 ± 0.034	2.253GlyTyr: 2.253 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	2.599HisAla: 2.599 ± 0.044	0.243HisCys: 0.243 ± 0.014	1.383HisAsp: 1.383 ± 0.035	1.002HisGlu: 1.002 ± 0.03	0.828HisPhe: 0.828 ± 0.023	2.039HisGly: 2.039 ± 0.043	0.59HisHis: 0.59 ± 0.024	0.891HisIle: 0.891 ± 0.026	0.482HisLys: 0.482 ± 0.021	2.31HisLeu: 2.31 ± 0.045	0.548HisMet: 0.548 ± 0.022	0.453HisAsn: 0.453 ± 0.022	1.447HisPro: 1.447 ± 0.036	0.61HisGln: 0.61 ± 0.023	1.428HisArg: 1.428 ± 0.04	0.914HisSer: 0.914 ± 0.032	0.745HisThr: 0.745 ± 0.026	1.716HisVal: 1.716 ± 0.039	0.344HisTrp: 0.344 ± 0.019	0.52HisTyr: 0.52 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	7.134IleAla: 7.134 ± 0.08	0.627IleCys: 0.627 ± 0.026	3.381IleAsp: 3.381 ± 0.055	3.352IleGlu: 3.352 ± 0.055	1.737IlePhe: 1.737 ± 0.044	4.959IleGly: 4.959 ± 0.07	0.978IleHis: 0.978 ± 0.032	2.302IleIle: 2.302 ± 0.055	1.359IleLys: 1.359 ± 0.038	5.02IleLeu: 5.02 ± 0.073	1.238IleMet: 1.238 ± 0.034	1.4IleAsn: 1.4 ± 0.038	2.44IlePro: 2.44 ± 0.045	1.169IleGln: 1.169 ± 0.029	3.483IleArg: 3.483 ± 0.049	2.905IleSer: 2.905 ± 0.048	3.064IleThr: 3.064 ± 0.058	3.742IleVal: 3.742 ± 0.065	0.742IleTrp: 0.742 ± 0.025	1.052IleTyr: 1.052 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	3.692LysAla: 3.692 ± 0.065	0.163LysCys: 0.163 ± 0.012	1.601LysAsp: 1.601 ± 0.039	1.182LysGlu: 1.182 ± 0.035	0.804LysPhe: 0.804 ± 0.025	2.696LysGly: 2.696 ± 0.046	0.539LysHis: 0.539 ± 0.022	1.477LysIle: 1.477 ± 0.039	0.967LysLys: 0.967 ± 0.03	2.627LysLeu: 2.627 ± 0.054	0.804LysMet: 0.804 ± 0.026	0.62LysAsn: 0.62 ± 0.027	1.7LysPro: 1.7 ± 0.054	0.838LysGln: 0.838 ± 0.028	2.013LysArg: 2.013 ± 0.048	1.516LysSer: 1.516 ± 0.043	1.818LysThr: 1.818 ± 0.047	1.988LysVal: 1.988 ± 0.044	0.341LysTrp: 0.341 ± 0.017	0.551LysTyr: 0.551 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	13.703LeuAla: 13.703 ± 0.14	0.933LeuCys: 0.933 ± 0.028	6.285LeuAsp: 6.285 ± 0.082	5.22LeuGlu: 5.22 ± 0.071	3.461LeuPhe: 3.461 ± 0.065	8.706LeuGly: 8.706 ± 0.094	2.043LeuHis: 2.043 ± 0.046	5.388LeuIle: 5.388 ± 0.076	2.907LeuLys: 2.907 ± 0.053	9.186LeuLeu: 9.186 ± 0.149	2.916LeuMet: 2.916 ± 0.051	2.595LeuAsn: 2.595 ± 0.058	5.846LeuPro: 5.846 ± 0.074	2.887LeuGln: 2.887 ± 0.056	7.435LeuArg: 7.435 ± 0.086	6.558LeuSer: 6.558 ± 0.078	6.218LeuThr: 6.218 ± 0.071	7.126LeuVal: 7.126 ± 0.087	1.279LeuTrp: 1.279 ± 0.037	1.893LeuTyr: 1.893 ± 0.044	0.0LeuXaa: 0.0 ± 0.0
Met	3.927MetAla: 3.927 ± 0.061	0.188MetCys: 0.188 ± 0.013	1.602MetAsp: 1.602 ± 0.037	1.348MetGlu: 1.348 ± 0.034	0.881MetPhe: 0.881 ± 0.028	2.541MetGly: 2.541 ± 0.05	0.515MetHis: 0.515 ± 0.021	1.765MetIle: 1.765 ± 0.043	0.961MetLys: 0.961 ± 0.028	2.864MetLeu: 2.864 ± 0.053	0.909MetMet: 0.909 ± 0.027	0.841MetAsn: 0.841 ± 0.025	1.657MetPro: 1.657 ± 0.037	1.191MetGln: 1.191 ± 0.034	2.152MetArg: 2.152 ± 0.048	1.702MetSer: 1.702 ± 0.038	2.32MetThr: 2.32 ± 0.043	2.069MetVal: 2.069 ± 0.042	0.218MetTrp: 0.218 ± 0.014	0.343MetTyr: 0.343 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.106AsnAla: 3.106 ± 0.059	0.226AsnCys: 0.226 ± 0.015	1.4AsnAsp: 1.4 ± 0.055	1.098AsnGlu: 1.098 ± 0.036	0.859AsnPhe: 0.859 ± 0.029	2.25AsnGly: 2.25 ± 0.049	0.463AsnHis: 0.463 ± 0.019	1.333AsnIle: 1.333 ± 0.037	0.581AsnLys: 0.581 ± 0.025	2.53AsnLeu: 2.53 ± 0.043	0.712AsnMet: 0.712 ± 0.023	0.661AsnAsn: 0.661 ± 0.027	1.845AsnPro: 1.845 ± 0.042	0.643AsnGln: 0.643 ± 0.025	1.782AsnArg: 1.782 ± 0.039	1.071AsnSer: 1.071 ± 0.037	1.327AsnThr: 1.327 ± 0.034	1.692AsnVal: 1.692 ± 0.041	0.378AsnTrp: 0.378 ± 0.017	0.594AsnTyr: 0.594 ± 0.024	0.0AsnXaa: 0.0 ± 0.0
Pro	6.587ProAla: 6.587 ± 0.098	0.362ProCys: 0.362 ± 0.018	4.148ProAsp: 4.148 ± 0.059	3.723ProGlu: 3.723 ± 0.064	1.963ProPhe: 1.963 ± 0.039	4.649ProGly: 4.649 ± 0.077	1.117ProHis: 1.117 ± 0.035	2.21ProIle: 2.21 ± 0.04	1.425ProLys: 1.425 ± 0.039	4.953ProLeu: 4.953 ± 0.066	1.4ProMet: 1.4 ± 0.035	1.171ProAsn: 1.171 ± 0.038	2.237ProPro: 2.237 ± 0.051	1.842ProGln: 1.842 ± 0.041	3.042ProArg: 3.042 ± 0.054	2.316ProSer: 2.316 ± 0.042	2.242ProThr: 2.242 ± 0.044	4.544ProVal: 4.544 ± 0.061	0.693ProTrp: 0.693 ± 0.026	1.138ProTyr: 1.138 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	4.673GlnAla: 4.673 ± 0.066	0.19GlnCys: 0.19 ± 0.013	2.018GlnAsp: 2.018 ± 0.045	1.421GlnGlu: 1.421 ± 0.039	1.087GlnPhe: 1.087 ± 0.028	3.003GlnGly: 3.003 ± 0.057	0.593GlnHis: 0.593 ± 0.025	2.172GlnIle: 2.172 ± 0.039	0.994GlnLys: 0.994 ± 0.031	2.753GlnLeu: 2.753 ± 0.052	1.176GlnMet: 1.176 ± 0.03	0.895GlnAsn: 0.895 ± 0.033	1.783GlnPro: 1.783 ± 0.04	1.13GlnGln: 1.13 ± 0.041	2.248GlnArg: 2.248 ± 0.048	1.762GlnSer: 1.762 ± 0.041	2.018GlnThr: 2.018 ± 0.043	2.54GlnVal: 2.54 ± 0.042	0.408GlnTrp: 0.408 ± 0.018	0.533GlnTyr: 0.533 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	9.011ArgAla: 9.011 ± 0.112	0.487ArgCys: 0.487 ± 0.021	4.61ArgAsp: 4.61 ± 0.071	3.517ArgGlu: 3.517 ± 0.058	2.745ArgPhe: 2.745 ± 0.054	4.773ArgGly: 4.773 ± 0.065	1.668ArgHis: 1.668 ± 0.038	4.21ArgIle: 4.21 ± 0.065	2.186ArgLys: 2.186 ± 0.047	7.832ArgLeu: 7.832 ± 0.108	2.246ArgMet: 2.246 ± 0.045	1.755ArgAsn: 1.755 ± 0.037	3.544ArgPro: 3.544 ± 0.059	2.533ArgGln: 2.533 ± 0.052	5.132ArgArg: 5.132 ± 0.085	3.175ArgSer: 3.175 ± 0.048	3.069ArgThr: 3.069 ± 0.049	4.809ArgVal: 4.809 ± 0.07	0.957ArgTrp: 0.957 ± 0.036	1.492ArgTyr: 1.492 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	5.589SerAla: 5.589 ± 0.061	0.415SerCys: 0.415 ± 0.02	3.066SerAsp: 3.066 ± 0.054	2.445SerGlu: 2.445 ± 0.045	2.096SerPhe: 2.096 ± 0.043	5.272SerGly: 5.272 ± 0.08	1.045SerHis: 1.045 ± 0.03	2.438SerIle: 2.438 ± 0.051	1.202SerLys: 1.202 ± 0.035	5.085SerLeu: 5.085 ± 0.073	1.399SerMet: 1.399 ± 0.032	1.239SerAsn: 1.239 ± 0.036	2.452SerPro: 2.452 ± 0.048	1.522SerGln: 1.522 ± 0.032	3.179SerArg: 3.179 ± 0.047	2.373SerSer: 2.373 ± 0.055	2.426SerThr: 2.426 ± 0.043	3.622SerVal: 3.622 ± 0.062	0.656SerTrp: 0.656 ± 0.024	1.137SerTyr: 1.137 ± 0.028	0.0SerXaa: 0.0 ± 0.0
Thr	6.435ThrAla: 6.435 ± 0.09	0.489ThrCys: 0.489 ± 0.021	3.21ThrAsp: 3.21 ± 0.054	2.855ThrGlu: 2.855 ± 0.055	1.9ThrPhe: 1.9 ± 0.042	5.619ThrGly: 5.619 ± 0.086	1.159ThrHis: 1.159 ± 0.032	2.778ThrIle: 2.778 ± 0.056	1.222ThrLys: 1.222 ± 0.035	5.977ThrLeu: 5.977 ± 0.072	1.376ThrMet: 1.376 ± 0.026	1.193ThrAsn: 1.193 ± 0.034	3.39ThrPro: 3.39 ± 0.049	1.685ThrGln: 1.685 ± 0.037	3.843ThrArg: 3.843 ± 0.061	2.574ThrSer: 2.574 ± 0.043	2.872ThrThr: 2.872 ± 0.059	4.163ThrVal: 4.163 ± 0.063	0.72ThrTrp: 0.72 ± 0.022	1.206ThrTyr: 1.206 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	9.151ValAla: 9.151 ± 0.097	0.626ValCys: 0.626 ± 0.026	4.023ValAsp: 4.023 ± 0.061	3.978ValGlu: 3.978 ± 0.06	2.844ValPhe: 2.844 ± 0.054	5.234ValGly: 5.234 ± 0.073	1.337ValHis: 1.337 ± 0.041	4.597ValIle: 4.597 ± 0.072	1.951ValLys: 1.951 ± 0.048	7.676ValLeu: 7.676 ± 0.085	2.409ValMet: 2.409 ± 0.049	1.971ValAsn: 1.971 ± 0.048	3.647ValPro: 3.647 ± 0.057	2.226ValGln: 2.226 ± 0.048	4.158ValArg: 4.158 ± 0.059	3.822ValSer: 3.822 ± 0.062	4.957ValThr: 4.957 ± 0.072	5.362ValVal: 5.362 ± 0.082	0.987ValTrp: 0.987 ± 0.026	1.386ValTyr: 1.386 ± 0.031	0.0ValXaa: 0.0 ± 0.0
Trp	1.38TrpAla: 1.38 ± 0.039	0.166TrpCys: 0.166 ± 0.012	0.749TrpAsp: 0.749 ± 0.027	0.599TrpGlu: 0.599 ± 0.024	0.553TrpPhe: 0.553 ± 0.024	1.033TrpGly: 1.033 ± 0.031	0.349TrpHis: 0.349 ± 0.021	0.713TrpIle: 0.713 ± 0.025	0.399TrpLys: 0.399 ± 0.021	1.64TrpLeu: 1.64 ± 0.041	0.449TrpMet: 0.449 ± 0.019	0.402TrpAsn: 0.402 ± 0.017	0.707TrpPro: 0.707 ± 0.024	0.62TrpGln: 0.62 ± 0.023	1.129TrpArg: 1.129 ± 0.03	0.819TrpSer: 0.819 ± 0.023	0.78TrpThr: 0.78 ± 0.025	0.89TrpVal: 0.89 ± 0.027	0.228TrpTrp: 0.228 ± 0.014	0.278TrpTyr: 0.278 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.481TyrAla: 2.481 ± 0.055	0.223TyrCys: 0.223 ± 0.014	1.499TyrAsp: 1.499 ± 0.037	1.118TyrGlu: 1.118 ± 0.033	0.817TyrPhe: 0.817 ± 0.027	2.085TyrGly: 2.085 ± 0.038	0.498TyrHis: 0.498 ± 0.022	0.87TyrIle: 0.87 ± 0.026	0.552TyrLys: 0.552 ± 0.026	2.232TyrLeu: 2.232 ± 0.045	0.497TyrMet: 0.497 ± 0.022	0.505TyrAsn: 0.505 ± 0.022	1.006TyrPro: 1.006 ± 0.029	0.608TyrGln: 0.608 ± 0.023	1.534TyrArg: 1.534 ± 0.037	0.987TyrSer: 0.987 ± 0.032	1.081TyrThr: 1.081 ± 0.032	1.457TyrVal: 1.457 ± 0.031	0.345TyrTrp: 0.345 ± 0.018	0.517TyrTyr: 0.517 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 3813 proteins (1208368 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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