Amino acid dipepetide frequency for Novosphingobium sp.

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.802AlaAla: 18.802 ± 0.182	1.078AlaCys: 1.078 ± 0.027	7.23AlaAsp: 7.23 ± 0.078	8.463AlaGlu: 8.463 ± 0.094	4.356AlaPhe: 4.356 ± 0.058	11.382AlaGly: 11.382 ± 0.113	2.229AlaHis: 2.229 ± 0.046	6.695AlaIle: 6.695 ± 0.066	4.334AlaLys: 4.334 ± 0.066	13.856AlaLeu: 13.856 ± 0.138	3.748AlaMet: 3.748 ± 0.055	3.172AlaAsn: 3.172 ± 0.059	6.401AlaPro: 6.401 ± 0.075	4.647AlaGln: 4.647 ± 0.068	9.658AlaArg: 9.658 ± 0.103	6.558AlaSer: 6.558 ± 0.092	6.555AlaThr: 6.555 ± 0.095	8.689AlaVal: 8.689 ± 0.095	1.764AlaTrp: 1.764 ± 0.035	2.615AlaTyr: 2.615 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	0.974CysAla: 0.974 ± 0.025	0.073CysCys: 0.073 ± 0.008	0.549CysAsp: 0.549 ± 0.02	0.443CysGlu: 0.443 ± 0.019	0.291CysPhe: 0.291 ± 0.014	0.849CysGly: 0.849 ± 0.024	0.241CysHis: 0.241 ± 0.019	0.343CysIle: 0.343 ± 0.017	0.167CysLys: 0.167 ± 0.011	0.709CysLeu: 0.709 ± 0.025	0.148CysMet: 0.148 ± 0.01	0.229CysAsn: 0.229 ± 0.013	0.45CysPro: 0.45 ± 0.018	0.205CysGln: 0.205 ± 0.012	0.549CysArg: 0.549 ± 0.02	0.437CysSer: 0.437 ± 0.019	0.407CysThr: 0.407 ± 0.02	0.583CysVal: 0.583 ± 0.022	0.095CysTrp: 0.095 ± 0.009	0.17CysTyr: 0.17 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.203AspAla: 7.203 ± 0.081	0.474AspCys: 0.474 ± 0.018	3.243AspAsp: 3.243 ± 0.057	3.487AspGlu: 3.487 ± 0.054	2.277AspPhe: 2.277 ± 0.039	5.507AspGly: 5.507 ± 0.076	1.355AspHis: 1.355 ± 0.035	2.647AspIle: 2.647 ± 0.046	1.882AspLys: 1.882 ± 0.042	5.931AspLeu: 5.931 ± 0.067	1.3AspMet: 1.3 ± 0.033	1.407AspAsn: 1.407 ± 0.04	3.661AspPro: 3.661 ± 0.052	1.789AspGln: 1.789 ± 0.037	4.751AspArg: 4.751 ± 0.058	2.31AspSer: 2.31 ± 0.047	2.64AspThr: 2.64 ± 0.05	4.038AspVal: 4.038 ± 0.057	1.201AspTrp: 1.201 ± 0.028	1.671AspTyr: 1.671 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	8.213GluAla: 8.213 ± 0.084	0.344GluCys: 0.344 ± 0.015	3.036GluAsp: 3.036 ± 0.051	3.04GluGlu: 3.04 ± 0.063	1.729GluPhe: 1.729 ± 0.039	4.6GluGly: 4.6 ± 0.064	1.276GluHis: 1.276 ± 0.032	3.225GluIle: 3.225 ± 0.049	1.905GluLys: 1.905 ± 0.045	5.479GluLeu: 5.479 ± 0.064	1.342GluMet: 1.342 ± 0.035	1.413GluAsn: 1.413 ± 0.033	2.7GluPro: 2.7 ± 0.046	2.091GluGln: 2.091 ± 0.041	4.847GluArg: 4.847 ± 0.074	2.319GluSer: 2.319 ± 0.044	3.367GluThr: 3.367 ± 0.053	3.938GluVal: 3.938 ± 0.061	0.826GluTrp: 0.826 ± 0.026	1.008GluTyr: 1.008 ± 0.028	0.0GluXaa: 0.0 ± 0.0
Phe	5.141PheAla: 5.141 ± 0.064	0.349PheCys: 0.349 ± 0.016	2.743PheAsp: 2.743 ± 0.041	2.049PheGlu: 2.049 ± 0.039	1.291PhePhe: 1.291 ± 0.034	3.734PheGly: 3.734 ± 0.056	0.785PheHis: 0.785 ± 0.027	1.285PheIle: 1.285 ± 0.034	0.921PheLys: 0.921 ± 0.028	3.01PheLeu: 3.01 ± 0.056	0.677PheMet: 0.677 ± 0.022	1.069PheAsn: 1.069 ± 0.026	1.536PhePro: 1.536 ± 0.036	0.959PheGln: 0.959 ± 0.025	2.207PheArg: 2.207 ± 0.048	2.036PheSer: 2.036 ± 0.035	2.116PheThr: 2.116 ± 0.046	2.786PheVal: 2.786 ± 0.047	0.536PheTrp: 0.536 ± 0.024	0.932PheTyr: 0.932 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	10.122GlyAla: 10.122 ± 0.097	0.77GlyCys: 0.77 ± 0.028	4.849GlyAsp: 4.849 ± 0.062	5.129GlyGlu: 5.129 ± 0.067	3.713GlyPhe: 3.713 ± 0.049	8.205GlyGly: 8.205 ± 0.088	1.772GlyHis: 1.772 ± 0.041	4.174GlyIle: 4.174 ± 0.061	3.528GlyLys: 3.528 ± 0.059	9.127GlyLeu: 9.127 ± 0.088	2.323GlyMet: 2.323 ± 0.05	2.344GlyAsn: 2.344 ± 0.053	3.739GlyPro: 3.739 ± 0.057	3.236GlyGln: 3.236 ± 0.051	6.241GlyArg: 6.241 ± 0.072	4.881GlySer: 4.881 ± 0.064	4.931GlyThr: 4.931 ± 0.082	6.308GlyVal: 6.308 ± 0.081	1.592GlyTrp: 1.592 ± 0.034	2.39GlyTyr: 2.39 ± 0.042	0.0GlyXaa: 0.0 ± 0.0
His	2.438HisAla: 2.438 ± 0.048	0.244HisCys: 0.244 ± 0.015	1.28HisAsp: 1.28 ± 0.037	1.06HisGlu: 1.06 ± 0.028	0.819HisPhe: 0.819 ± 0.025	1.994HisGly: 1.994 ± 0.037	0.547HisHis: 0.547 ± 0.022	0.819HisIle: 0.819 ± 0.025	0.457HisLys: 0.457 ± 0.017	1.906HisLeu: 1.906 ± 0.04	0.433HisMet: 0.433 ± 0.019	0.457HisAsn: 0.457 ± 0.018	1.311HisPro: 1.311 ± 0.037	0.556HisGln: 0.556 ± 0.018	1.488HisArg: 1.488 ± 0.038	0.914HisSer: 0.914 ± 0.028	0.769HisThr: 0.769 ± 0.023	1.498HisVal: 1.498 ± 0.036	0.341HisTrp: 0.341 ± 0.017	0.567HisTyr: 0.567 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	7.583IleAla: 7.583 ± 0.091	0.444IleCys: 0.444 ± 0.019	3.895IleAsp: 3.895 ± 0.06	3.589IleGlu: 3.589 ± 0.061	1.332IlePhe: 1.332 ± 0.036	4.974IleGly: 4.974 ± 0.072	0.848IleHis: 0.848 ± 0.027	1.685IleIle: 1.685 ± 0.046	1.302IleLys: 1.302 ± 0.039	3.508IleLeu: 3.508 ± 0.049	0.823IleMet: 0.823 ± 0.028	1.375IleAsn: 1.375 ± 0.037	2.224IlePro: 2.224 ± 0.043	1.192IleGln: 1.192 ± 0.027	2.978IleArg: 2.978 ± 0.047	2.527IleSer: 2.527 ± 0.051	2.571IleThr: 2.571 ± 0.079	4.053IleVal: 4.053 ± 0.06	0.567IleTrp: 0.567 ± 0.022	0.947IleTyr: 0.947 ± 0.028	0.0IleXaa: 0.0 ± 0.0
Lys	4.058LysAla: 4.058 ± 0.057	0.13LysCys: 0.13 ± 0.011	1.647LysAsp: 1.647 ± 0.042	1.187LysGlu: 1.187 ± 0.035	0.941LysPhe: 0.941 ± 0.025	2.739LysGly: 2.739 ± 0.05	0.567LysHis: 0.567 ± 0.022	1.505LysIle: 1.505 ± 0.039	0.988LysLys: 0.988 ± 0.035	3.401LysLeu: 3.401 ± 0.052	0.693LysMet: 0.693 ± 0.025	0.72LysAsn: 0.72 ± 0.027	2.121LysPro: 2.121 ± 0.048	0.965LysGln: 0.965 ± 0.029	2.224LysArg: 2.224 ± 0.043	1.543LysSer: 1.543 ± 0.035	1.748LysThr: 1.748 ± 0.039	2.352LysVal: 2.352 ± 0.045	0.386LysTrp: 0.386 ± 0.017	0.623LysTyr: 0.623 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	14.621LeuAla: 14.621 ± 0.132	0.78LeuCys: 0.78 ± 0.023	6.116LeuAsp: 6.116 ± 0.076	5.005LeuGlu: 5.005 ± 0.064	3.543LeuPhe: 3.543 ± 0.063	8.613LeuGly: 8.613 ± 0.081	1.857LeuHis: 1.857 ± 0.038	4.658LeuIle: 4.658 ± 0.065	2.983LeuLys: 2.983 ± 0.05	9.234LeuLeu: 9.234 ± 0.102	2.034LeuMet: 2.034 ± 0.043	2.365LeuAsn: 2.365 ± 0.041	5.649LeuPro: 5.649 ± 0.073	2.595LeuGln: 2.595 ± 0.044	7.002LeuArg: 7.002 ± 0.08	5.943LeuSer: 5.943 ± 0.076	5.805LeuThr: 5.805 ± 0.07	7.254LeuVal: 7.254 ± 0.069	1.157LeuTrp: 1.157 ± 0.035	1.902LeuTyr: 1.902 ± 0.038	0.0LeuXaa: 0.0 ± 0.0
Met	3.067MetAla: 3.067 ± 0.046	0.153MetCys: 0.153 ± 0.01	1.091MetAsp: 1.091 ± 0.033	1.01MetGlu: 1.01 ± 0.028	0.653MetPhe: 0.653 ± 0.022	1.827MetGly: 1.827 ± 0.043	0.43MetHis: 0.43 ± 0.018	1.239MetIle: 1.239 ± 0.028	0.819MetLys: 0.819 ± 0.025	2.469MetLeu: 2.469 ± 0.042	0.578MetMet: 0.578 ± 0.022	0.66MetAsn: 0.66 ± 0.022	1.482MetPro: 1.482 ± 0.035	0.746MetGln: 0.746 ± 0.024	1.659MetArg: 1.659 ± 0.037	1.378MetSer: 1.378 ± 0.029	1.715MetThr: 1.715 ± 0.035	1.526MetVal: 1.526 ± 0.035	0.226MetTrp: 0.226 ± 0.015	0.239MetTyr: 0.239 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.244AsnAla: 3.244 ± 0.049	0.244AsnCys: 0.244 ± 0.014	1.442AsnAsp: 1.442 ± 0.056	1.155AsnGlu: 1.155 ± 0.033	1.041AsnPhe: 1.041 ± 0.033	2.458AsnGly: 2.458 ± 0.071	0.5AsnHis: 0.5 ± 0.018	1.246AsnIle: 1.246 ± 0.035	0.649AsnLys: 0.649 ± 0.024	2.575AsnLeu: 2.575 ± 0.047	0.53AsnMet: 0.53 ± 0.019	0.735AsnAsn: 0.735 ± 0.026	1.832AsnPro: 1.832 ± 0.04	0.798AsnGln: 0.798 ± 0.027	1.813AsnArg: 1.813 ± 0.035	1.256AsnSer: 1.256 ± 0.03	1.243AsnThr: 1.243 ± 0.037	1.962AsnVal: 1.962 ± 0.042	0.448AsnTrp: 0.448 ± 0.021	0.747AsnTyr: 0.747 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	7.136ProAla: 7.136 ± 0.08	0.301ProCys: 0.301 ± 0.015	3.418ProAsp: 3.418 ± 0.054	3.867ProGlu: 3.867 ± 0.057	1.9ProPhe: 1.9 ± 0.039	4.922ProGly: 4.922 ± 0.069	1.047ProHis: 1.047 ± 0.032	2.471ProIle: 2.471 ± 0.046	1.571ProLys: 1.571 ± 0.04	5.136ProLeu: 5.136 ± 0.062	1.138ProMet: 1.138 ± 0.027	1.355ProAsn: 1.355 ± 0.035	2.659ProPro: 2.659 ± 0.065	1.816ProGln: 1.816 ± 0.037	3.245ProArg: 3.245 ± 0.053	2.752ProSer: 2.752 ± 0.047	2.654ProThr: 2.654 ± 0.065	4.221ProVal: 4.221 ± 0.066	0.706ProTrp: 0.706 ± 0.021	1.059ProTyr: 1.059 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	4.55GlnAla: 4.55 ± 0.063	0.218GlnCys: 0.218 ± 0.012	1.578GlnAsp: 1.578 ± 0.031	1.392GlnGlu: 1.392 ± 0.032	1.161GlnPhe: 1.161 ± 0.03	2.682GlnGly: 2.682 ± 0.043	0.633GlnHis: 0.633 ± 0.02	1.796GlnIle: 1.796 ± 0.041	0.923GlnLys: 0.923 ± 0.027	3.093GlnLeu: 3.093 ± 0.05	0.813GlnMet: 0.813 ± 0.026	0.787GlnAsn: 0.787 ± 0.026	1.857GlnPro: 1.857 ± 0.043	1.178GlnGln: 1.178 ± 0.035	2.467GlnArg: 2.467 ± 0.046	1.68GlnSer: 1.68 ± 0.039	1.63GlnThr: 1.63 ± 0.035	2.51GlnVal: 2.51 ± 0.046	0.462GlnTrp: 0.462 ± 0.019	0.675GlnTyr: 0.675 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	8.486ArgAla: 8.486 ± 0.087	0.491ArgCys: 0.491 ± 0.021	4.274ArgAsp: 4.274 ± 0.059	4.321ArgGlu: 4.321 ± 0.07	3.082ArgPhe: 3.082 ± 0.057	5.353ArgGly: 5.353 ± 0.064	1.624ArgHis: 1.624 ± 0.042	3.736ArgIle: 3.736 ± 0.058	2.155ArgLys: 2.155 ± 0.042	7.938ArgLeu: 7.938 ± 0.091	1.826ArgMet: 1.826 ± 0.035	1.828ArgAsn: 1.828 ± 0.04	3.55ArgPro: 3.55 ± 0.061	2.626ArgGln: 2.626 ± 0.045	5.533ArgArg: 5.533 ± 0.083	3.632ArgSer: 3.632 ± 0.055	3.286ArgThr: 3.286 ± 0.048	4.817ArgVal: 4.817 ± 0.057	1.181ArgTrp: 1.181 ± 0.033	1.862ArgTyr: 1.862 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	6.377SerAla: 6.377 ± 0.074	0.425SerCys: 0.425 ± 0.019	2.964SerAsp: 2.964 ± 0.049	2.708SerGlu: 2.708 ± 0.043	2.177SerPhe: 2.177 ± 0.04	5.294SerGly: 5.294 ± 0.063	1.027SerHis: 1.027 ± 0.029	2.466SerIle: 2.466 ± 0.056	1.406SerLys: 1.406 ± 0.036	5.328SerLeu: 5.328 ± 0.065	1.094SerMet: 1.094 ± 0.029	1.407SerAsn: 1.407 ± 0.034	2.917SerPro: 2.917 ± 0.052	1.706SerGln: 1.706 ± 0.034	3.507SerArg: 3.507 ± 0.052	2.754SerSer: 2.754 ± 0.052	2.633SerThr: 2.633 ± 0.051	3.629SerVal: 3.629 ± 0.056	0.744SerTrp: 0.744 ± 0.025	1.384SerTyr: 1.384 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	6.642ThrAla: 6.642 ± 0.093	0.409ThrCys: 0.409 ± 0.017	2.801ThrAsp: 2.801 ± 0.052	2.485ThrGlu: 2.485 ± 0.043	1.953ThrPhe: 1.953 ± 0.048	5.438ThrGly: 5.438 ± 0.072	0.94ThrHis: 0.94 ± 0.027	3.073ThrIle: 3.073 ± 0.069	1.431ThrLys: 1.431 ± 0.036	5.603ThrLeu: 5.603 ± 0.08	1.146ThrMet: 1.146 ± 0.03	1.375ThrAsn: 1.375 ± 0.036	3.458ThrPro: 3.458 ± 0.071	1.555ThrGln: 1.555 ± 0.032	3.427ThrArg: 3.427 ± 0.055	2.78ThrSer: 2.78 ± 0.053	2.885ThrThr: 2.885 ± 0.052	4.327ThrVal: 4.327 ± 0.09	0.684ThrTrp: 0.684 ± 0.025	1.239ThrTyr: 1.239 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	9.535ValAla: 9.535 ± 0.089	0.607ValCys: 0.607 ± 0.022	4.245ValAsp: 4.245 ± 0.055	4.467ValGlu: 4.467 ± 0.057	2.422ValPhe: 2.422 ± 0.045	5.602ValGly: 5.602 ± 0.08	1.33ValHis: 1.33 ± 0.033	3.875ValIle: 3.875 ± 0.061	2.021ValLys: 2.021 ± 0.042	7.007ValLeu: 7.007 ± 0.086	1.61ValMet: 1.61 ± 0.039	2.075ValAsn: 2.075 ± 0.06	4.026ValPro: 4.026 ± 0.049	2.021ValGln: 2.021 ± 0.032	4.829ValArg: 4.829 ± 0.063	4.168ValSer: 4.168 ± 0.057	4.75ValThr: 4.75 ± 0.071	5.601ValVal: 5.601 ± 0.082	0.921ValTrp: 0.921 ± 0.025	1.415ValTyr: 1.415 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.347TrpAla: 1.347 ± 0.033	0.135TrpCys: 0.135 ± 0.01	0.799TrpAsp: 0.799 ± 0.025	0.62TrpGlu: 0.62 ± 0.023	0.572TrpPhe: 0.572 ± 0.022	1.008TrpGly: 1.008 ± 0.032	0.388TrpHis: 0.388 ± 0.019	0.697TrpIle: 0.697 ± 0.022	0.474TrpLys: 0.474 ± 0.019	1.769TrpLeu: 1.769 ± 0.038	0.309TrpMet: 0.309 ± 0.014	0.494TrpAsn: 0.494 ± 0.02	0.755TrpPro: 0.755 ± 0.024	0.686TrpGln: 0.686 ± 0.024	1.328TrpArg: 1.328 ± 0.033	0.843TrpSer: 0.843 ± 0.028	0.809TrpThr: 0.809 ± 0.028	0.828TrpVal: 0.828 ± 0.025	0.274TrpTrp: 0.274 ± 0.015	0.312TrpTyr: 0.312 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.691TyrAla: 2.691 ± 0.047	0.241TyrCys: 0.241 ± 0.014	1.573TyrAsp: 1.573 ± 0.034	1.22TyrGlu: 1.22 ± 0.028	0.91TyrPhe: 0.91 ± 0.03	2.195TyrGly: 2.195 ± 0.046	0.503TyrHis: 0.503 ± 0.02	0.784TyrIle: 0.784 ± 0.026	0.624TyrLys: 0.624 ± 0.021	2.035TyrLeu: 2.035 ± 0.039	0.403TyrMet: 0.403 ± 0.017	0.647TyrAsn: 0.647 ± 0.023	1.048TyrPro: 1.048 ± 0.029	0.717TyrGln: 0.717 ± 0.024	1.847TyrArg: 1.847 ± 0.041	1.236TyrSer: 1.236 ± 0.033	1.137TyrThr: 1.137 ± 0.045	1.61TyrVal: 1.61 ± 0.034	0.334TyrTrp: 0.334 ± 0.015	0.606TyrTyr: 0.606 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4332 proteins (1345091 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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