Toxoplasma gondii (strain ATCC 50861 / VEG)

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia; Eucoccidiorida; Eimeriorina; Sarcocystidae; Toxoplasma; Toxoplasma gondii; Toxoplasma gondii type III

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8404 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V4YK67|V4YK67_TOXGV Uncharacterized protein (Fragment) OS=Toxoplasma gondii (strain ATCC 50861 / VEG) OX=432359 GN=TGVEG_361850 PE=4 SV=1
MM1 pKa = 7.02SAEE4 pKa = 4.27RR5 pKa = 11.84QSSSCDD11 pKa = 3.32CVLEE15 pKa = 4.32ATDD18 pKa = 4.28CASGTGNEE26 pKa = 4.14GVAFAVDD33 pKa = 3.72SFRR36 pKa = 11.84GFLPLEE42 pKa = 4.48VVCDD46 pKa = 4.35CRR48 pKa = 11.84PSPCDD53 pKa = 3.4GTLEE57 pKa = 4.42PADD60 pKa = 4.41PMDD63 pKa = 5.66SPGTWLVNDD72 pKa = 4.39FDD74 pKa = 4.99VDD76 pKa = 3.91FLRR79 pKa = 11.84ARR81 pKa = 11.84FPLEE85 pKa = 3.56EE86 pKa = 4.03TSAEE90 pKa = 4.11RR91 pKa = 11.84QSSSCDD97 pKa = 3.32CVLEE101 pKa = 4.32ATDD104 pKa = 4.28CASGTGNEE112 pKa = 4.14GVAFAVDD119 pKa = 3.72SFRR122 pKa = 11.84GFLPLEE128 pKa = 4.48VVCDD132 pKa = 4.35CRR134 pKa = 11.84PSPCDD139 pKa = 3.4GTLEE143 pKa = 4.42PADD146 pKa = 4.41PMDD149 pKa = 5.66SPGTWLVDD157 pKa = 3.68DD158 pKa = 5.07FDD160 pKa = 5.08VDD162 pKa = 3.88FLRR165 pKa = 11.84ARR167 pKa = 11.84FPLEE171 pKa = 3.56EE172 pKa = 4.03TSAEE176 pKa = 4.11RR177 pKa = 11.84QSSSCDD183 pKa = 3.32CVLEE187 pKa = 4.32ATDD190 pKa = 4.28CASGTGNEE198 pKa = 4.14GVAFAVDD205 pKa = 3.72SFRR208 pKa = 11.84GFLPLEE214 pKa = 4.48VVCDD218 pKa = 4.35CRR220 pKa = 11.84PSPCDD225 pKa = 3.4GTLEE229 pKa = 4.42PADD232 pKa = 4.41PMDD235 pKa = 5.66SPGTWLVDD243 pKa = 3.68DD244 pKa = 5.07FDD246 pKa = 5.08VDD248 pKa = 3.88FLRR251 pKa = 11.84ARR253 pKa = 11.84FPLGEE258 pKa = 4.1MSAEE262 pKa = 4.08RR263 pKa = 11.84QSSSCDD269 pKa = 3.32CVLEE273 pKa = 4.32ATDD276 pKa = 4.28CASGTGNEE284 pKa = 4.14GVAFAVDD291 pKa = 3.72SFRR294 pKa = 11.84GFLPLEE300 pKa = 4.48VVCDD304 pKa = 4.35CRR306 pKa = 11.84PSPCDD311 pKa = 3.4GTLEE315 pKa = 4.42PADD318 pKa = 4.57PMDD321 pKa = 4.16SPP323 pKa = 4.78

Molecular weight: 34.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A125YSL9|A0A125YSL9_TOXGV Uncharacterized protein OS=Toxoplasma gondii (strain ATCC 50861 / VEG) OX=432359 GN=BN1205_038190 PE=4 SV=1
MM1 pKa = 7.65LKK3 pKa = 10.06HH4 pKa = 6.47GSPIAQPRR12 pKa = 11.84RR13 pKa = 11.84SSRR16 pKa = 11.84VRR18 pKa = 11.84LPRR21 pKa = 11.84YY22 pKa = 8.1RR23 pKa = 11.84ARR25 pKa = 11.84RR26 pKa = 11.84YY27 pKa = 10.85KK28 pKa = 9.75MDD30 pKa = 3.32STRR33 pKa = 11.84SSMSNEE39 pKa = 3.79AGLGQAVSHH48 pKa = 6.04QSTVSSSKK56 pKa = 10.2NPVFGGEE63 pKa = 4.3FFCLANPSATAAVFKK78 pKa = 10.71ASRR81 pKa = 11.84CARR84 pKa = 11.84CYY86 pKa = 10.62GPRR89 pKa = 11.84LSEE92 pKa = 3.69QGPFPRR98 pKa = 11.84EE99 pKa = 3.45EE100 pKa = 4.03ARR102 pKa = 11.84SVRR105 pKa = 11.84HH106 pKa = 5.22VRR108 pKa = 11.84PPGNPCGADD117 pKa = 3.26SQPRR121 pKa = 11.84CRR123 pKa = 11.84LPRR126 pKa = 11.84RR127 pKa = 11.84EE128 pKa = 4.11APRR131 pKa = 11.84GSRR134 pKa = 11.84GDD136 pKa = 3.55PGSPSGGQKK145 pKa = 9.76SAPGKK150 pKa = 9.23NVRR153 pKa = 11.84SNSDD157 pKa = 2.76SWPDD161 pKa = 3.28SRR163 pKa = 11.84NRR165 pKa = 11.84LPDD168 pKa = 3.59SQPTSASEE176 pKa = 4.2RR177 pKa = 11.84DD178 pKa = 3.68TGSPPLYY185 pKa = 10.57GCLCPRR191 pKa = 11.84SKK193 pKa = 10.9RR194 pKa = 11.84LGRR197 pKa = 11.84ASGRR201 pKa = 11.84AHH203 pKa = 7.12FYY205 pKa = 9.56ASEE208 pKa = 3.78WRR210 pKa = 11.84GGRR213 pKa = 11.84RR214 pKa = 11.84VFRR217 pKa = 11.84ASIEE221 pKa = 4.08PVGALGNKK229 pKa = 9.6SSATPRR235 pKa = 11.84KK236 pKa = 9.16GIRR239 pKa = 11.84VCGMEE244 pKa = 4.4LRR246 pKa = 11.84LNTPHH251 pKa = 7.09CPPPGCALLLGVLLLAASLLAVLFPSDD278 pKa = 3.39QVSRR282 pKa = 11.84HH283 pKa = 4.92IGKK286 pKa = 10.19FSFLNAISATEE297 pKa = 4.1LPQCLKK303 pKa = 10.83LAFSTSLGPHH313 pKa = 5.86PAWRR317 pKa = 11.84AVASAVFPLCGLAAGVAVVFFLFRR341 pKa = 11.84RR342 pKa = 11.84PYY344 pKa = 9.89IVEE347 pKa = 3.7QSLEE351 pKa = 3.75IVANFGVQLQQKK363 pKa = 6.31TRR365 pKa = 11.84WGGIKK370 pKa = 10.04RR371 pKa = 11.84QFIDD375 pKa = 3.41ASQVSDD381 pKa = 3.63VIINEE386 pKa = 4.03GFRR389 pKa = 11.84ACDD392 pKa = 3.23VVFYY396 pKa = 10.98VALLVKK402 pKa = 9.56DD403 pKa = 3.92QPSMVVPFQHH413 pKa = 6.67FPLPLEE419 pKa = 4.43EE420 pKa = 4.42NLVIYY425 pKa = 7.59EE426 pKa = 4.3TLHH429 pKa = 6.14HH430 pKa = 6.85LLHH433 pKa = 6.74ISSGVRR439 pKa = 11.84RR440 pKa = 11.84AA441 pKa = 3.2

Molecular weight: 48.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8404

0

8404

6625207

26

15897

788.3

85.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.209 ± 0.029

2.032 ± 0.014

4.726 ± 0.013

7.709 ± 0.034

3.475 ± 0.012

7.441 ± 0.032

2.181 ± 0.009

2.373 ± 0.015

4.018 ± 0.024

9.006 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.4 ± 0.009

2.322 ± 0.013

6.464 ± 0.024

3.958 ± 0.015

8.265 ± 0.033

11.085 ± 0.041

5.042 ± 0.015

5.831 ± 0.018

1.014 ± 0.006

1.449 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski