Mycobacterium phage OkiRoe

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A059VGD4|A0A059VGD4_9CAUD Uncharacterized protein OS=Mycobacterium phage OkiRoe OX=1486473 GN=51 PE=4 SV=1
MM1 pKa = 7.41TSHH4 pKa = 6.91AMPLCTEE11 pKa = 4.02CEE13 pKa = 4.03LYY15 pKa = 10.88YY16 pKa = 10.41ATSFDD21 pKa = 4.18PDD23 pKa = 3.27GSMMCGDD30 pKa = 4.53CRR32 pKa = 11.84AADD35 pKa = 4.6DD36 pKa = 4.5EE37 pKa = 4.58PQATEE42 pKa = 5.23AGDD45 pKa = 3.61PDD47 pKa = 3.43KK48 pKa = 10.95WIVQGDD54 pKa = 3.73TGEE57 pKa = 4.51LMTIYY62 pKa = 9.71PSVVTPPIPAPAAWIVSAAGEE83 pKa = 4.16VTVPVEE89 pKa = 4.15AFAPAVPAWISEE101 pKa = 3.97LVRR104 pKa = 11.84LMSTPIDD111 pKa = 3.48TAVFLPEE118 pKa = 4.07PADD121 pKa = 4.59FIDD124 pKa = 3.98TSEE127 pKa = 4.34EE128 pKa = 3.97PLPIDD133 pKa = 3.9IDD135 pKa = 4.07DD136 pKa = 4.42PAEE139 pKa = 4.13DD140 pKa = 4.02VVNHH144 pKa = 6.39PSHH147 pKa = 5.74YY148 pKa = 8.15TSHH151 pKa = 7.29PSGVEE156 pKa = 3.89CITITEE162 pKa = 4.12HH163 pKa = 6.35MGFNLGNAVKK173 pKa = 10.04YY174 pKa = 10.22IWRR177 pKa = 11.84CDD179 pKa = 3.69LKK181 pKa = 10.9RR182 pKa = 11.84DD183 pKa = 4.51AIEE186 pKa = 4.44DD187 pKa = 3.62LKK189 pKa = 11.27KK190 pKa = 10.47AAFYY194 pKa = 10.84VDD196 pKa = 3.95RR197 pKa = 11.84EE198 pKa = 3.8IAKK201 pKa = 10.17RR202 pKa = 11.84EE203 pKa = 3.91RR204 pKa = 11.84EE205 pKa = 3.98LAAGGQQ211 pKa = 3.53

Molecular weight: 22.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A059VHC6|A0A059VHC6_9CAUD Uncharacterized protein OS=Mycobacterium phage OkiRoe OX=1486473 GN=89 PE=4 SV=1
MM1 pKa = 7.36GVTRR5 pKa = 11.84AARR8 pKa = 11.84PAVDD12 pKa = 3.17WMNAANARR20 pKa = 11.84VRR22 pKa = 11.84EE23 pKa = 4.56LTRR26 pKa = 11.84DD27 pKa = 3.42MYY29 pKa = 11.26PDD31 pKa = 3.47VEE33 pKa = 4.48VTQRR37 pKa = 11.84DD38 pKa = 3.66LCIVASCCTAGTHH51 pKa = 5.15SRR53 pKa = 11.84IRR55 pKa = 11.84RR56 pKa = 11.84LHH58 pKa = 5.47GEE60 pKa = 4.02KK61 pKa = 10.22VRR63 pKa = 11.84QYY65 pKa = 9.3NPWVFGVDD73 pKa = 3.64YY74 pKa = 10.83LGTWRR79 pKa = 11.84EE80 pKa = 3.95TWPQWLRR87 pKa = 11.84RR88 pKa = 11.84MTRR91 pKa = 11.84EE92 pKa = 3.82GFHH95 pKa = 6.11PWKK98 pKa = 10.23VRR100 pKa = 11.84KK101 pKa = 8.63PP102 pKa = 3.51

Molecular weight: 12.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

19529

43

1369

203.4

22.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.673 ± 0.439

1.08 ± 0.138

6.636 ± 0.205

5.612 ± 0.261

2.668 ± 0.142

8.792 ± 0.443

2.227 ± 0.19

4.153 ± 0.17

3.354 ± 0.168

7.927 ± 0.191

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.115 ± 0.11

2.852 ± 0.192

5.469 ± 0.232

3.441 ± 0.159

7.481 ± 0.358

4.982 ± 0.255

6.682 ± 0.214

7.763 ± 0.212

1.925 ± 0.128

2.166 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski