Amino acid dipepetide frequency for Mycobacterium phage OkiRoe

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.893AlaAla: 20.893 ± 1.704
0.922AlaCys: 0.922 ± 0.187
7.835AlaAsp: 7.835 ± 0.502
7.835AlaGlu: 7.835 ± 0.871
3.329AlaPhe: 3.329 ± 0.58
12.853AlaGly: 12.853 ± 1.084
2.458AlaHis: 2.458 ± 0.399
5.274AlaIle: 5.274 ± 0.581
3.994AlaLys: 3.994 ± 0.52
10.242AlaLeu: 10.242 ± 0.778
2.97AlaMet: 2.97 ± 0.437
2.612AlaAsn: 2.612 ± 0.442
5.531AlaPro: 5.531 ± 0.613
4.865AlaGln: 4.865 ± 0.528
8.449AlaArg: 8.449 ± 0.713
5.633AlaSer: 5.633 ± 0.681
7.169AlaThr: 7.169 ± 0.705
9.013AlaVal: 9.013 ± 0.88
2.612AlaTrp: 2.612 ± 0.421
2.253AlaTyr: 2.253 ± 0.345
0.0AlaXaa: 0.0 ± 0.0
Cys
1.331CysAla: 1.331 ± 0.271
0.154CysCys: 0.154 ± 0.082
0.973CysAsp: 0.973 ± 0.235
0.512CysGlu: 0.512 ± 0.152
0.102CysPhe: 0.102 ± 0.082
1.587CysGly: 1.587 ± 0.366
0.358CysHis: 0.358 ± 0.136
0.563CysIle: 0.563 ± 0.156
0.461CysLys: 0.461 ± 0.131
0.358CysLeu: 0.358 ± 0.16
0.154CysMet: 0.154 ± 0.085
0.205CysAsn: 0.205 ± 0.097
0.666CysPro: 0.666 ± 0.197
0.256CysGln: 0.256 ± 0.112
0.358CysArg: 0.358 ± 0.121
0.768CysSer: 0.768 ± 0.241
0.819CysThr: 0.819 ± 0.215
0.666CysVal: 0.666 ± 0.194
0.358CysTrp: 0.358 ± 0.123
0.154CysTyr: 0.154 ± 0.095
0.0CysXaa: 0.0 ± 0.0
Asp
8.603AspAla: 8.603 ± 0.696
0.973AspCys: 0.973 ± 0.257
5.94AspAsp: 5.94 ± 0.635
5.07AspGlu: 5.07 ± 0.543
1.229AspPhe: 1.229 ± 0.237
6.299AspGly: 6.299 ± 0.581
1.024AspHis: 1.024 ± 0.217
2.202AspIle: 2.202 ± 0.373
2.253AspLys: 2.253 ± 0.345
6.145AspLeu: 6.145 ± 0.525
1.331AspMet: 1.331 ± 0.266
1.587AspAsn: 1.587 ± 0.285
3.482AspPro: 3.482 ± 0.402
1.536AspGln: 1.536 ± 0.246
4.967AspArg: 4.967 ± 0.565
1.741AspSer: 1.741 ± 0.254
4.302AspThr: 4.302 ± 0.417
5.274AspVal: 5.274 ± 0.422
0.871AspTrp: 0.871 ± 0.209
1.536AspTyr: 1.536 ± 0.252
0.0AspXaa: 0.0 ± 0.0
Glu
7.169GluAla: 7.169 ± 0.739
0.717GluCys: 0.717 ± 0.209
3.585GluAsp: 3.585 ± 0.437
2.765GluGlu: 2.765 ± 0.409
1.741GluPhe: 1.741 ± 0.384
3.636GluGly: 3.636 ± 0.494
1.69GluHis: 1.69 ± 0.333
1.536GluIle: 1.536 ± 0.276
1.69GluLys: 1.69 ± 0.335
6.043GluLeu: 6.043 ± 0.652
1.485GluMet: 1.485 ± 0.207
0.973GluAsn: 0.973 ± 0.195
2.868GluPro: 2.868 ± 0.465
2.612GluGln: 2.612 ± 0.364
5.07GluArg: 5.07 ± 0.734
2.714GluSer: 2.714 ± 0.378
2.868GluThr: 2.868 ± 0.429
4.558GluVal: 4.558 ± 0.497
1.024GluTrp: 1.024 ± 0.196
1.383GluTyr: 1.383 ± 0.258
0.0GluXaa: 0.0 ± 0.0
Phe
3.226PheAla: 3.226 ± 0.469
0.205PheCys: 0.205 ± 0.089
2.663PheAsp: 2.663 ± 0.384
1.536PheGlu: 1.536 ± 0.26
0.563PhePhe: 0.563 ± 0.129
2.97PheGly: 2.97 ± 0.297
0.563PheHis: 0.563 ± 0.174
1.536PheIle: 1.536 ± 0.267
0.922PheLys: 0.922 ± 0.238
1.639PheLeu: 1.639 ± 0.312
0.563PheMet: 0.563 ± 0.157
0.922PheAsn: 0.922 ± 0.264
1.434PhePro: 1.434 ± 0.258
0.563PheGln: 0.563 ± 0.181
1.383PheArg: 1.383 ± 0.218
1.075PheSer: 1.075 ± 0.281
2.151PheThr: 2.151 ± 0.357
1.792PheVal: 1.792 ± 0.308
0.41PheTrp: 0.41 ± 0.165
0.563PheTyr: 0.563 ± 0.151
0.0PheXaa: 0.0 ± 0.0
Gly
9.269GlyAla: 9.269 ± 1.068
1.024GlyCys: 1.024 ± 0.25
5.633GlyAsp: 5.633 ± 0.494
5.377GlyGlu: 5.377 ± 0.621
2.509GlyPhe: 2.509 ± 0.376
9.371GlyGly: 9.371 ± 1.126
1.639GlyHis: 1.639 ± 0.332
3.892GlyIle: 3.892 ± 0.398
3.226GlyLys: 3.226 ± 0.362
5.787GlyLeu: 5.787 ± 0.8
1.792GlyMet: 1.792 ± 0.306
3.431GlyAsn: 3.431 ± 0.535
3.789GlyPro: 3.789 ± 0.502
2.868GlyGln: 2.868 ± 0.369
6.862GlyArg: 6.862 ± 0.576
4.455GlySer: 4.455 ± 0.504
6.913GlyThr: 6.913 ± 0.627
6.555GlyVal: 6.555 ± 0.458
1.639GlyTrp: 1.639 ± 0.28
1.895GlyTyr: 1.895 ± 0.317
0.0GlyXaa: 0.0 ± 0.0
His
2.407HisAla: 2.407 ± 0.383
0.358HisCys: 0.358 ± 0.129
1.331HisAsp: 1.331 ± 0.233
0.973HisGlu: 0.973 ± 0.221
0.461HisPhe: 0.461 ± 0.187
2.356HisGly: 2.356 ± 0.387
0.666HisHis: 0.666 ± 0.171
1.178HisIle: 1.178 ± 0.294
0.666HisLys: 0.666 ± 0.197
2.151HisLeu: 2.151 ± 0.282
0.563HisMet: 0.563 ± 0.153
0.461HisAsn: 0.461 ± 0.145
1.434HisPro: 1.434 ± 0.291
0.358HisGln: 0.358 ± 0.13
1.639HisArg: 1.639 ± 0.344
1.331HisSer: 1.331 ± 0.248
1.178HisThr: 1.178 ± 0.285
1.844HisVal: 1.844 ± 0.33
0.41HisTrp: 0.41 ± 0.174
0.512HisTyr: 0.512 ± 0.155
0.0HisXaa: 0.0 ± 0.0
Ile
5.479IleAla: 5.479 ± 0.544
0.256IleCys: 0.256 ± 0.128
3.636IleAsp: 3.636 ± 0.403
3.277IleGlu: 3.277 ± 0.511
0.615IlePhe: 0.615 ± 0.159
4.045IleGly: 4.045 ± 0.442
0.512IleHis: 0.512 ± 0.169
1.229IleIle: 1.229 ± 0.238
1.587IleLys: 1.587 ± 0.286
2.407IleLeu: 2.407 ± 0.284
0.461IleMet: 0.461 ± 0.169
1.741IleAsn: 1.741 ± 0.352
2.509IlePro: 2.509 ± 0.512
1.127IleGln: 1.127 ± 0.195
2.816IleArg: 2.816 ± 0.359
1.792IleSer: 1.792 ± 0.271
2.663IleThr: 2.663 ± 0.37
3.943IleVal: 3.943 ± 0.421
0.717IleTrp: 0.717 ± 0.243
0.717IleTyr: 0.717 ± 0.142
0.0IleXaa: 0.0 ± 0.0
Lys
3.841LysAla: 3.841 ± 0.436
0.358LysCys: 0.358 ± 0.129
0.666LysAsp: 0.666 ± 0.198
0.871LysGlu: 0.871 ± 0.176
1.178LysPhe: 1.178 ± 0.242
1.946LysGly: 1.946 ± 0.441
0.871LysHis: 0.871 ± 0.24
1.383LysIle: 1.383 ± 0.309
1.024LysLys: 1.024 ± 0.222
3.738LysLeu: 3.738 ± 0.438
0.922LysMet: 0.922 ± 0.212
1.127LysAsn: 1.127 ± 0.28
2.868LysPro: 2.868 ± 0.511
1.792LysGln: 1.792 ± 0.349
3.021LysArg: 3.021 ± 0.359
1.741LysSer: 1.741 ± 0.301
1.997LysThr: 1.997 ± 0.295
2.612LysVal: 2.612 ± 0.358
0.819LysTrp: 0.819 ± 0.177
0.768LysTyr: 0.768 ± 0.196
0.0LysXaa: 0.0 ± 0.0
Leu
10.549LeuAla: 10.549 ± 0.604
1.331LeuCys: 1.331 ± 0.267
5.735LeuAsp: 5.735 ± 0.56
3.021LeuGlu: 3.021 ± 0.372
2.407LeuPhe: 2.407 ± 0.331
6.094LeuGly: 6.094 ± 0.68
2.356LeuHis: 2.356 ± 0.443
3.329LeuIle: 3.329 ± 0.389
3.124LeuLys: 3.124 ± 0.383
5.274LeuLeu: 5.274 ± 0.487
1.639LeuMet: 1.639 ± 0.27
2.151LeuAsn: 2.151 ± 0.296
4.609LeuPro: 4.609 ± 0.558
3.124LeuGln: 3.124 ± 0.468
6.196LeuArg: 6.196 ± 0.715
4.404LeuSer: 4.404 ± 0.513
5.582LeuThr: 5.582 ± 0.508
5.377LeuVal: 5.377 ± 0.611
1.485LeuTrp: 1.485 ± 0.272
1.485LeuTyr: 1.485 ± 0.257
0.0LeuXaa: 0.0 ± 0.0
Met
1.946MetAla: 1.946 ± 0.26
0.102MetCys: 0.102 ± 0.061
0.819MetAsp: 0.819 ± 0.208
0.615MetGlu: 0.615 ± 0.165
0.717MetPhe: 0.717 ± 0.197
1.639MetGly: 1.639 ± 0.299
0.41MetHis: 0.41 ± 0.124
0.871MetIle: 0.871 ± 0.227
0.461MetLys: 0.461 ± 0.197
2.202MetLeu: 2.202 ± 0.361
0.563MetMet: 0.563 ± 0.186
0.819MetAsn: 0.819 ± 0.176
1.229MetPro: 1.229 ± 0.229
0.819MetGln: 0.819 ± 0.252
1.383MetArg: 1.383 ± 0.283
2.1MetSer: 2.1 ± 0.408
2.458MetThr: 2.458 ± 0.305
1.28MetVal: 1.28 ± 0.257
0.205MetTrp: 0.205 ± 0.104
0.512MetTyr: 0.512 ± 0.165
0.0MetXaa: 0.0 ± 0.0
Asn
3.789AsnAla: 3.789 ± 0.62
0.256AsnCys: 0.256 ± 0.114
1.485AsnAsp: 1.485 ± 0.287
0.922AsnGlu: 0.922 ± 0.199
0.461AsnPhe: 0.461 ± 0.252
3.585AsnGly: 3.585 ± 0.41
0.563AsnHis: 0.563 ± 0.16
0.922AsnIle: 0.922 ± 0.234
0.922AsnLys: 0.922 ± 0.231
2.253AsnLeu: 2.253 ± 0.373
0.563AsnMet: 0.563 ± 0.142
0.819AsnAsn: 0.819 ± 0.192
2.612AsnPro: 2.612 ± 0.323
0.819AsnGln: 0.819 ± 0.214
1.946AsnArg: 1.946 ± 0.306
0.871AsnSer: 0.871 ± 0.212
1.946AsnThr: 1.946 ± 0.314
2.407AsnVal: 2.407 ± 0.293
0.717AsnTrp: 0.717 ± 0.195
0.666AsnTyr: 0.666 ± 0.178
0.0AsnXaa: 0.0 ± 0.0
Pro
8.705ProAla: 8.705 ± 0.738
0.256ProCys: 0.256 ± 0.117
3.994ProAsp: 3.994 ± 0.501
3.021ProGlu: 3.021 ± 0.293
1.536ProPhe: 1.536 ± 0.259
4.66ProGly: 4.66 ± 0.542
1.434ProHis: 1.434 ± 0.256
2.202ProIle: 2.202 ± 0.287
1.946ProLys: 1.946 ± 0.297
3.789ProLeu: 3.789 ± 0.536
1.229ProMet: 1.229 ± 0.258
1.383ProAsn: 1.383 ± 0.221
2.509ProPro: 2.509 ± 0.365
2.202ProGln: 2.202 ± 0.4
3.38ProArg: 3.38 ± 0.466
2.816ProSer: 2.816 ± 0.44
3.329ProThr: 3.329 ± 0.34
4.25ProVal: 4.25 ± 0.474
0.922ProTrp: 0.922 ± 0.242
1.075ProTyr: 1.075 ± 0.214
0.0ProXaa: 0.0 ± 0.0
Gln
4.353GlnAla: 4.353 ± 0.482
0.205GlnCys: 0.205 ± 0.101
1.178GlnAsp: 1.178 ± 0.245
1.741GlnGlu: 1.741 ± 0.274
1.024GlnPhe: 1.024 ± 0.201
1.639GlnGly: 1.639 ± 0.271
0.768GlnHis: 0.768 ± 0.226
1.844GlnIle: 1.844 ± 0.305
1.229GlnLys: 1.229 ± 0.232
3.073GlnLeu: 3.073 ± 0.459
0.922GlnMet: 0.922 ± 0.226
1.024GlnAsn: 1.024 ± 0.254
2.202GlnPro: 2.202 ± 0.299
1.383GlnGln: 1.383 ± 0.287
2.97GlnArg: 2.97 ± 0.395
1.741GlnSer: 1.741 ± 0.299
2.1GlnThr: 2.1 ± 0.288
2.919GlnVal: 2.919 ± 0.392
1.178GlnTrp: 1.178 ± 0.233
0.922GlnTyr: 0.922 ± 0.204
0.0GlnXaa: 0.0 ± 0.0
Arg
7.528ArgAla: 7.528 ± 0.801
1.28ArgCys: 1.28 ± 0.265
4.455ArgAsp: 4.455 ± 0.454
4.353ArgGlu: 4.353 ± 0.504
1.997ArgPhe: 1.997 ± 0.287
5.991ArgGly: 5.991 ± 0.643
1.844ArgHis: 1.844 ± 0.312
4.148ArgIle: 4.148 ± 0.374
2.97ArgLys: 2.97 ± 0.351
6.299ArgLeu: 6.299 ± 0.553
1.946ArgMet: 1.946 ± 0.314
2.048ArgAsn: 2.048 ± 0.327
3.636ArgPro: 3.636 ± 0.497
3.073ArgGln: 3.073 ± 0.317
6.811ArgArg: 6.811 ± 0.826
4.045ArgSer: 4.045 ± 0.537
4.455ArgThr: 4.455 ± 0.447
4.814ArgVal: 4.814 ± 0.538
1.331ArgTrp: 1.331 ± 0.221
1.792ArgTyr: 1.792 ± 0.312
0.0ArgXaa: 0.0 ± 0.0
Ser
6.452SerAla: 6.452 ± 0.678
0.461SerCys: 0.461 ± 0.165
3.585SerAsp: 3.585 ± 0.45
2.765SerGlu: 2.765 ± 0.459
1.075SerPhe: 1.075 ± 0.21
4.609SerGly: 4.609 ± 0.692
0.922SerHis: 0.922 ± 0.278
2.304SerIle: 2.304 ± 0.408
1.536SerLys: 1.536 ± 0.289
2.304SerLeu: 2.304 ± 0.333
1.485SerMet: 1.485 ± 0.254
1.639SerAsn: 1.639 ± 0.298
2.202SerPro: 2.202 ± 0.375
1.331SerGln: 1.331 ± 0.352
3.021SerArg: 3.021 ± 0.428
3.021SerSer: 3.021 ± 0.581
4.097SerThr: 4.097 ± 0.477
4.66SerVal: 4.66 ± 0.647
1.331SerTrp: 1.331 ± 0.326
1.024SerTyr: 1.024 ± 0.204
0.0SerXaa: 0.0 ± 0.0
Thr
8.193ThrAla: 8.193 ± 0.673
0.563ThrCys: 0.563 ± 0.21
3.994ThrAsp: 3.994 ± 0.548
4.097ThrGlu: 4.097 ± 0.594
2.151ThrPhe: 2.151 ± 0.356
5.991ThrGly: 5.991 ± 0.664
1.639ThrHis: 1.639 ± 0.313
3.226ThrIle: 3.226 ± 0.353
1.69ThrLys: 1.69 ± 0.303
5.428ThrLeu: 5.428 ± 0.547
0.819ThrMet: 0.819 ± 0.205
1.69ThrAsn: 1.69 ± 0.283
4.302ThrPro: 4.302 ± 0.439
1.741ThrGln: 1.741 ± 0.259
4.711ThrArg: 4.711 ± 0.605
2.97ThrSer: 2.97 ± 0.49
3.892ThrThr: 3.892 ± 0.521
6.657ThrVal: 6.657 ± 0.522
1.331ThrTrp: 1.331 ± 0.294
1.741ThrTyr: 1.741 ± 0.291
0.0ThrXaa: 0.0 ± 0.0
Val
8.91ValAla: 8.91 ± 0.821
0.768ValCys: 0.768 ± 0.217
6.503ValAsp: 6.503 ± 0.59
5.07ValGlu: 5.07 ± 0.568
2.458ValPhe: 2.458 ± 0.379
5.991ValGly: 5.991 ± 0.593
1.895ValHis: 1.895 ± 0.278
2.356ValIle: 2.356 ± 0.331
2.816ValLys: 2.816 ± 0.42
5.889ValLeu: 5.889 ± 0.604
1.28ValMet: 1.28 ± 0.209
2.509ValAsn: 2.509 ± 0.324
4.404ValPro: 4.404 ± 0.465
2.151ValGln: 2.151 ± 0.358
5.787ValArg: 5.787 ± 0.584
4.045ValSer: 4.045 ± 0.522
5.838ValThr: 5.838 ± 0.548
6.247ValVal: 6.247 ± 0.564
1.178ValTrp: 1.178 ± 0.213
1.536ValTyr: 1.536 ± 0.322
0.0ValXaa: 0.0 ± 0.0
Trp
1.946TrpAla: 1.946 ± 0.376
0.461TrpCys: 0.461 ± 0.178
1.075TrpAsp: 1.075 ± 0.291
0.973TrpGlu: 0.973 ± 0.225
0.615TrpPhe: 0.615 ± 0.182
0.973TrpGly: 0.973 ± 0.258
0.358TrpHis: 0.358 ± 0.143
0.666TrpIle: 0.666 ± 0.208
0.563TrpLys: 0.563 ± 0.149
2.202TrpLeu: 2.202 ± 0.297
0.205TrpMet: 0.205 ± 0.095
0.768TrpAsn: 0.768 ± 0.142
1.127TrpPro: 1.127 ± 0.24
0.819TrpGln: 0.819 ± 0.237
1.895TrpArg: 1.895 ± 0.322
1.075TrpSer: 1.075 ± 0.225
1.434TrpThr: 1.434 ± 0.284
1.28TrpVal: 1.28 ± 0.226
0.307TrpTrp: 0.307 ± 0.152
0.512TrpTyr: 0.512 ± 0.168
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.151TyrAla: 2.151 ± 0.348
0.154TyrCys: 0.154 ± 0.087
1.28TyrAsp: 1.28 ± 0.301
1.331TyrGlu: 1.331 ± 0.294
0.512TyrPhe: 0.512 ± 0.129
1.69TyrGly: 1.69 ± 0.344
0.307TyrHis: 0.307 ± 0.116
0.871TyrIle: 0.871 ± 0.237
0.461TyrLys: 0.461 ± 0.146
2.048TyrLeu: 2.048 ± 0.294
0.205TyrMet: 0.205 ± 0.094
0.615TyrAsn: 0.615 ± 0.159
1.28TyrPro: 1.28 ± 0.254
0.973TyrGln: 0.973 ± 0.215
2.151TyrArg: 2.151 ± 0.342
1.485TyrSer: 1.485 ± 0.293
1.639TyrThr: 1.639 ± 0.346
1.485TyrVal: 1.485 ± 0.277
0.41TyrTrp: 0.41 ± 0.141
0.615TyrTyr: 0.615 ± 0.216
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 96 proteins (19529 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski