Propionibacterium phage PFR1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pulverervirus; Propionibacterium virus PFR1

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A173G9D0|A0A173G9D0_9CAUD Integrase OS=Propionibacterium phage PFR1 OX=1838137 GN=PFR1_26 PE=3 SV=1
MM1 pKa = 7.7TDD3 pKa = 2.96STDD6 pKa = 3.36TTFSTRR12 pKa = 11.84DD13 pKa = 3.19EE14 pKa = 5.01AIASIIDD21 pKa = 4.16AIEE24 pKa = 4.13AGGAVTDD31 pKa = 4.05ARR33 pKa = 11.84SEE35 pKa = 4.12YY36 pKa = 10.87DD37 pKa = 3.2LDD39 pKa = 6.05AIADD43 pKa = 3.98EE44 pKa = 4.79LVTLHH49 pKa = 7.24SEE51 pKa = 4.25EE52 pKa = 4.3TSEE55 pKa = 4.27GATIASSVYY64 pKa = 9.71FAIDD68 pKa = 3.3ADD70 pKa = 4.39EE71 pKa = 4.46DD72 pKa = 4.19AFWAAVADD80 pKa = 4.02HH81 pKa = 6.63EE82 pKa = 4.91LVGITAQFTSINTPTVIDD100 pKa = 3.91GRR102 pKa = 11.84KK103 pKa = 5.32TTTDD107 pKa = 3.42TITIEE112 pKa = 4.06RR113 pKa = 11.84DD114 pKa = 3.45GVEE117 pKa = 4.05LDD119 pKa = 4.45AIDD122 pKa = 4.1VEE124 pKa = 4.63SSEE127 pKa = 4.88DD128 pKa = 3.45AEE130 pKa = 4.85PYY132 pKa = 10.69DD133 pKa = 3.79DD134 pKa = 4.36AVRR137 pKa = 11.84SIIGDD142 pKa = 3.58QPFTWVRR149 pKa = 3.22

Molecular weight: 16.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A173G9E5|A0A173G9E5_9CAUD Single-stranded DNA binding protein OS=Propionibacterium phage PFR1 OX=1838137 GN=PFR_50 PE=3 SV=1
MM1 pKa = 7.5SSFVSSPWRR10 pKa = 11.84DD11 pKa = 3.13IASLRR16 pKa = 11.84SLKK19 pKa = 10.57APRR22 pKa = 11.84RR23 pKa = 11.84TWRR26 pKa = 11.84QRR28 pKa = 11.84MVRR31 pKa = 11.84RR32 pKa = 11.84STIWAAMDD40 pKa = 3.32GTTCRR45 pKa = 11.84LNWTQSEE52 pKa = 4.32LTEE55 pKa = 4.06KK56 pKa = 9.91NRR58 pKa = 11.84RR59 pKa = 11.84MVALDD64 pKa = 3.04VLARR68 pKa = 11.84QIEE71 pKa = 4.39LAHH74 pKa = 5.84YY75 pKa = 7.81PQIRR79 pKa = 11.84EE80 pKa = 3.64WRR82 pKa = 11.84GNGRR86 pKa = 11.84PTCARR91 pKa = 11.84CGSSWPCQRR100 pKa = 11.84ARR102 pKa = 11.84DD103 pKa = 3.86AVRR106 pKa = 11.84LRR108 pKa = 11.84EE109 pKa = 4.18IIGGGDD115 pKa = 3.42DD116 pKa = 4.45PGDD119 pKa = 4.07DD120 pKa = 3.99GARR123 pKa = 11.84DD124 pKa = 4.6LIPEE128 pKa = 4.24VTAQMPTTRR137 pKa = 11.84TEE139 pKa = 3.93PATGATQQ146 pKa = 3.13

Molecular weight: 16.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

11993

68

1164

214.2

23.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.341 ± 0.423

0.842 ± 0.131

7.246 ± 0.337

4.819 ± 0.3

2.56 ± 0.256

8.338 ± 0.434

1.934 ± 0.16

4.503 ± 0.328

3.577 ± 0.451

7.763 ± 0.387

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.41 ± 0.121

2.301 ± 0.274

5.562 ± 0.336

3.41 ± 0.186

6.846 ± 0.401

5.87 ± 0.266

7.229 ± 0.356

7.521 ± 0.331

2.677 ± 0.202

1.251 ± 0.12
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski