Amino acid dipepetide frequency for Propionibacterium phage PFR1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.264AlaAla: 20.264 ± 1.77
0.834AlaCys: 0.834 ± 0.271
9.673AlaAsp: 9.673 ± 1.087
6.421AlaGlu: 6.421 ± 0.781
2.752AlaPhe: 2.752 ± 0.596
11.508AlaGly: 11.508 ± 0.932
2.252AlaHis: 2.252 ± 0.444
6.338AlaIle: 6.338 ± 0.924
5.254AlaLys: 5.254 ± 1.031
11.258AlaLeu: 11.258 ± 1.266
4.086AlaMet: 4.086 ± 0.709
3.252AlaAsn: 3.252 ± 0.702
5.67AlaPro: 5.67 ± 0.637
4.169AlaGln: 4.169 ± 0.741
8.422AlaArg: 8.422 ± 0.896
7.422AlaSer: 7.422 ± 0.75
9.84AlaThr: 9.84 ± 1.04
9.506AlaVal: 9.506 ± 0.74
3.085AlaTrp: 3.085 ± 0.567
1.418AlaTyr: 1.418 ± 0.3
0.0AlaXaa: 0.0 ± 0.0
Cys
0.917CysAla: 0.917 ± 0.324
0.167CysCys: 0.167 ± 0.117
0.5CysAsp: 0.5 ± 0.154
0.5CysGlu: 0.5 ± 0.196
0.083CysPhe: 0.083 ± 0.091
1.084CysGly: 1.084 ± 0.396
0.5CysHis: 0.5 ± 0.206
0.167CysIle: 0.167 ± 0.113
0.0CysLys: 0.0 ± 0.0
0.5CysLeu: 0.5 ± 0.216
0.167CysMet: 0.167 ± 0.103
0.25CysAsn: 0.25 ± 0.13
0.751CysPro: 0.751 ± 0.302
0.334CysGln: 0.334 ± 0.149
0.667CysArg: 0.667 ± 0.241
0.25CysSer: 0.25 ± 0.149
0.917CysThr: 0.917 ± 0.249
0.167CysVal: 0.167 ± 0.093
0.334CysTrp: 0.334 ± 0.164
0.167CysTyr: 0.167 ± 0.119
0.0CysXaa: 0.0 ± 0.0
Asp
11.007AspAla: 11.007 ± 1.167
0.417AspCys: 0.417 ± 0.204
5.587AspAsp: 5.587 ± 0.76
4.003AspGlu: 4.003 ± 0.792
1.751AspPhe: 1.751 ± 0.294
7.672AspGly: 7.672 ± 0.644
1.835AspHis: 1.835 ± 0.361
3.419AspIle: 3.419 ± 0.455
2.168AspLys: 2.168 ± 0.396
4.253AspLeu: 4.253 ± 0.703
1.334AspMet: 1.334 ± 0.313
1.501AspAsn: 1.501 ± 0.428
4.753AspPro: 4.753 ± 0.739
2.168AspGln: 2.168 ± 0.537
4.003AspArg: 4.003 ± 0.68
3.336AspSer: 3.336 ± 0.454
3.836AspThr: 3.836 ± 0.546
6.588AspVal: 6.588 ± 0.74
1.918AspTrp: 1.918 ± 0.334
0.917AspTyr: 0.917 ± 0.325
0.0AspXaa: 0.0 ± 0.0
Glu
6.171GluAla: 6.171 ± 0.742
0.5GluCys: 0.5 ± 0.245
2.668GluAsp: 2.668 ± 0.576
1.668GluGlu: 1.668 ± 0.373
1.584GluPhe: 1.584 ± 0.416
3.336GluGly: 3.336 ± 0.447
1.167GluHis: 1.167 ± 0.299
2.001GluIle: 2.001 ± 0.396
1.418GluLys: 1.418 ± 0.341
5.17GluLeu: 5.17 ± 0.953
1.501GluMet: 1.501 ± 0.338
0.917GluAsn: 0.917 ± 0.245
2.752GluPro: 2.752 ± 0.512
1.918GluGln: 1.918 ± 0.477
3.669GluArg: 3.669 ± 0.657
2.752GluSer: 2.752 ± 0.484
2.752GluThr: 2.752 ± 0.333
2.919GluVal: 2.919 ± 0.557
2.502GluTrp: 2.502 ± 0.467
0.834GluTyr: 0.834 ± 0.312
0.0GluXaa: 0.0 ± 0.0
Phe
3.419PheAla: 3.419 ± 0.432
0.584PheCys: 0.584 ± 0.225
2.085PheAsp: 2.085 ± 0.488
1.584PheGlu: 1.584 ± 0.454
0.25PhePhe: 0.25 ± 0.127
2.585PheGly: 2.585 ± 0.515
0.167PheHis: 0.167 ± 0.12
1.167PheIle: 1.167 ± 0.283
1.501PheLys: 1.501 ± 0.54
1.001PheLeu: 1.001 ± 0.236
0.5PheMet: 0.5 ± 0.194
0.584PheAsn: 0.584 ± 0.222
1.001PhePro: 1.001 ± 0.298
0.751PheGln: 0.751 ± 0.265
1.334PheArg: 1.334 ± 0.312
0.917PheSer: 0.917 ± 0.269
2.752PheThr: 2.752 ± 0.434
2.085PheVal: 2.085 ± 0.433
0.834PheTrp: 0.834 ± 0.273
0.5PheTyr: 0.5 ± 0.192
0.0PheXaa: 0.0 ± 0.0
Gly
10.09GlyAla: 10.09 ± 0.975
1.084GlyCys: 1.084 ± 0.233
5.837GlyAsp: 5.837 ± 0.653
3.419GlyGlu: 3.419 ± 0.679
2.668GlyPhe: 2.668 ± 0.351
7.255GlyGly: 7.255 ± 1.034
1.584GlyHis: 1.584 ± 0.361
3.419GlyIle: 3.419 ± 0.655
2.835GlyLys: 2.835 ± 0.669
8.339GlyLeu: 8.339 ± 0.942
2.668GlyMet: 2.668 ± 0.429
2.252GlyAsn: 2.252 ± 0.609
3.669GlyPro: 3.669 ± 0.526
2.585GlyGln: 2.585 ± 0.438
5.921GlyArg: 5.921 ± 0.759
4.086GlySer: 4.086 ± 0.443
6.171GlyThr: 6.171 ± 0.56
5.837GlyVal: 5.837 ± 0.809
2.585GlyTrp: 2.585 ± 0.549
1.084GlyTyr: 1.084 ± 0.244
0.0GlyXaa: 0.0 ± 0.0
His
2.001HisAla: 2.001 ± 0.422
0.0HisCys: 0.0 ± 0.0
1.668HisAsp: 1.668 ± 0.409
0.751HisGlu: 0.751 ± 0.294
0.5HisPhe: 0.5 ± 0.197
1.668HisGly: 1.668 ± 0.351
0.584HisHis: 0.584 ± 0.24
1.251HisIle: 1.251 ± 0.328
0.667HisLys: 0.667 ± 0.201
1.584HisLeu: 1.584 ± 0.374
0.417HisMet: 0.417 ± 0.204
0.25HisAsn: 0.25 ± 0.149
1.418HisPro: 1.418 ± 0.336
0.667HisGln: 0.667 ± 0.264
1.167HisArg: 1.167 ± 0.364
0.834HisSer: 0.834 ± 0.264
1.501HisThr: 1.501 ± 0.322
1.835HisVal: 1.835 ± 0.407
0.334HisTrp: 0.334 ± 0.169
0.25HisTyr: 0.25 ± 0.119
0.0HisXaa: 0.0 ± 0.0
Ile
5.921IleAla: 5.921 ± 0.537
0.417IleCys: 0.417 ± 0.187
4.253IleAsp: 4.253 ± 0.659
3.252IleGlu: 3.252 ± 0.552
0.5IlePhe: 0.5 ± 0.196
3.836IleGly: 3.836 ± 1.196
0.834IleHis: 0.834 ± 0.301
1.668IleIle: 1.668 ± 0.485
3.085IleLys: 3.085 ± 0.722
2.585IleLeu: 2.585 ± 0.571
0.584IleMet: 0.584 ± 0.175
0.917IleAsn: 0.917 ± 0.235
1.418IlePro: 1.418 ± 0.336
1.501IleGln: 1.501 ± 0.385
3.919IleArg: 3.919 ± 0.648
2.668IleSer: 2.668 ± 0.66
4.086IleThr: 4.086 ± 0.512
2.668IleVal: 2.668 ± 0.435
0.5IleTrp: 0.5 ± 0.163
0.417IleTyr: 0.417 ± 0.155
0.0IleXaa: 0.0 ± 0.0
Lys
5.42LysAla: 5.42 ± 0.844
0.0LysCys: 0.0 ± 0.0
1.668LysAsp: 1.668 ± 0.527
1.668LysGlu: 1.668 ± 0.375
1.167LysPhe: 1.167 ± 0.37
2.502LysGly: 2.502 ± 0.541
1.001LysHis: 1.001 ± 0.341
1.751LysIle: 1.751 ± 0.401
0.917LysLys: 0.917 ± 0.325
2.585LysLeu: 2.585 ± 0.464
0.584LysMet: 0.584 ± 0.213
0.667LysAsn: 0.667 ± 0.292
2.585LysPro: 2.585 ± 0.491
0.917LysGln: 0.917 ± 0.336
2.585LysArg: 2.585 ± 0.706
3.169LysSer: 3.169 ± 0.685
3.252LysThr: 3.252 ± 0.512
2.418LysVal: 2.418 ± 0.627
0.5LysTrp: 0.5 ± 0.179
0.417LysTyr: 0.417 ± 0.163
0.0LysXaa: 0.0 ± 0.0
Leu
9.923LeuAla: 9.923 ± 0.969
0.25LeuCys: 0.25 ± 0.176
5.754LeuAsp: 5.754 ± 0.925
2.835LeuGlu: 2.835 ± 0.456
2.335LeuPhe: 2.335 ± 0.524
7.755LeuGly: 7.755 ± 0.784
1.167LeuHis: 1.167 ± 0.349
3.586LeuIle: 3.586 ± 0.521
2.668LeuLys: 2.668 ± 0.488
5.087LeuLeu: 5.087 ± 0.797
1.751LeuMet: 1.751 ± 0.393
1.918LeuAsn: 1.918 ± 0.502
5.087LeuPro: 5.087 ± 0.594
1.918LeuGln: 1.918 ± 0.359
6.504LeuArg: 6.504 ± 0.861
5.17LeuSer: 5.17 ± 0.607
5.087LeuThr: 5.087 ± 0.84
6.254LeuVal: 6.254 ± 0.601
1.751LeuTrp: 1.751 ± 0.396
0.834LeuTyr: 0.834 ± 0.252
0.0LeuXaa: 0.0 ± 0.0
Met
3.919MetAla: 3.919 ± 0.644
0.083MetCys: 0.083 ± 0.082
1.167MetAsp: 1.167 ± 0.273
0.834MetGlu: 0.834 ± 0.287
0.5MetPhe: 0.5 ± 0.202
1.668MetGly: 1.668 ± 0.416
0.584MetHis: 0.584 ± 0.208
1.084MetIle: 1.084 ± 0.295
0.584MetLys: 0.584 ± 0.232
1.584MetLeu: 1.584 ± 0.328
0.417MetMet: 0.417 ± 0.176
1.084MetAsn: 1.084 ± 0.318
1.334MetPro: 1.334 ± 0.32
0.667MetGln: 0.667 ± 0.227
2.001MetArg: 2.001 ± 0.382
2.168MetSer: 2.168 ± 0.43
2.418MetThr: 2.418 ± 0.449
1.751MetVal: 1.751 ± 0.359
0.083MetTrp: 0.083 ± 0.077
0.167MetTyr: 0.167 ± 0.123
0.0MetXaa: 0.0 ± 0.0
Asn
3.919AsnAla: 3.919 ± 0.605
0.083AsnCys: 0.083 ± 0.078
1.501AsnAsp: 1.501 ± 0.327
0.751AsnGlu: 0.751 ± 0.261
0.584AsnPhe: 0.584 ± 0.213
2.085AsnGly: 2.085 ± 0.446
0.083AsnHis: 0.083 ± 0.077
1.084AsnIle: 1.084 ± 0.357
0.584AsnLys: 0.584 ± 0.184
1.835AsnLeu: 1.835 ± 0.417
0.25AsnMet: 0.25 ± 0.162
0.417AsnAsn: 0.417 ± 0.196
1.835AsnPro: 1.835 ± 0.267
0.751AsnGln: 0.751 ± 0.25
1.584AsnArg: 1.584 ± 0.432
1.835AsnSer: 1.835 ± 0.457
1.668AsnThr: 1.668 ± 0.404
1.334AsnVal: 1.334 ± 0.335
0.584AsnTrp: 0.584 ± 0.294
0.25AsnTyr: 0.25 ± 0.119
0.0AsnXaa: 0.0 ± 0.0
Pro
7.171ProAla: 7.171 ± 0.845
0.667ProCys: 0.667 ± 0.237
5.42ProAsp: 5.42 ± 0.794
3.002ProGlu: 3.002 ± 0.514
1.001ProPhe: 1.001 ± 0.315
4.503ProGly: 4.503 ± 0.673
0.917ProHis: 0.917 ± 0.262
2.168ProIle: 2.168 ± 0.357
2.168ProLys: 2.168 ± 0.397
3.586ProLeu: 3.586 ± 0.703
0.917ProMet: 0.917 ± 0.27
1.167ProAsn: 1.167 ± 0.328
3.252ProPro: 3.252 ± 0.566
1.501ProGln: 1.501 ± 0.318
3.002ProArg: 3.002 ± 0.529
3.336ProSer: 3.336 ± 0.472
4.086ProThr: 4.086 ± 0.596
5.42ProVal: 5.42 ± 0.656
1.751ProTrp: 1.751 ± 0.418
0.584ProTyr: 0.584 ± 0.193
0.0ProXaa: 0.0 ± 0.0
Gln
4.503GlnAla: 4.503 ± 0.761
0.167GlnCys: 0.167 ± 0.123
1.668GlnAsp: 1.668 ± 0.442
1.334GlnGlu: 1.334 ± 0.329
0.834GlnPhe: 0.834 ± 0.206
2.502GlnGly: 2.502 ± 0.352
1.084GlnHis: 1.084 ± 0.281
1.334GlnIle: 1.334 ± 0.397
0.834GlnLys: 0.834 ± 0.275
3.753GlnLeu: 3.753 ± 0.732
1.001GlnMet: 1.001 ± 0.289
0.417GlnAsn: 0.417 ± 0.165
2.085GlnPro: 2.085 ± 0.337
1.751GlnGln: 1.751 ± 0.369
2.168GlnArg: 2.168 ± 0.51
1.418GlnSer: 1.418 ± 0.289
2.502GlnThr: 2.502 ± 0.452
3.169GlnVal: 3.169 ± 0.55
0.834GlnTrp: 0.834 ± 0.263
0.751GlnTyr: 0.751 ± 0.262
0.0GlnXaa: 0.0 ± 0.0
Arg
9.006ArgAla: 9.006 ± 1.265
0.751ArgCys: 0.751 ± 0.319
5.17ArgAsp: 5.17 ± 0.56
4.169ArgGlu: 4.169 ± 0.657
2.085ArgPhe: 2.085 ± 0.405
4.086ArgGly: 4.086 ± 0.554
1.668ArgHis: 1.668 ± 0.437
3.085ArgIle: 3.085 ± 0.43
2.001ArgLys: 2.001 ± 0.494
4.753ArgLeu: 4.753 ± 0.567
1.001ArgMet: 1.001 ± 0.342
1.751ArgAsn: 1.751 ± 0.405
3.919ArgPro: 3.919 ± 0.559
3.336ArgGln: 3.336 ± 0.588
5.587ArgArg: 5.587 ± 0.96
4.086ArgSer: 4.086 ± 0.604
4.086ArgThr: 4.086 ± 0.617
4.253ArgVal: 4.253 ± 0.565
2.418ArgTrp: 2.418 ± 0.51
1.167ArgTyr: 1.167 ± 0.248
0.0ArgXaa: 0.0 ± 0.0
Ser
7.255SerAla: 7.255 ± 0.767
0.334SerCys: 0.334 ± 0.183
4.42SerAsp: 4.42 ± 0.457
3.085SerGlu: 3.085 ± 0.553
2.001SerPhe: 2.001 ± 0.444
5.587SerGly: 5.587 ± 0.567
1.084SerHis: 1.084 ± 0.279
2.502SerIle: 2.502 ± 0.428
1.584SerLys: 1.584 ± 0.286
4.67SerLeu: 4.67 ± 0.646
2.502SerMet: 2.502 ± 0.416
1.334SerAsn: 1.334 ± 0.373
3.085SerPro: 3.085 ± 0.632
1.501SerGln: 1.501 ± 0.345
3.002SerArg: 3.002 ± 0.646
4.086SerSer: 4.086 ± 0.844
3.753SerThr: 3.753 ± 0.487
4.837SerVal: 4.837 ± 0.646
1.501SerTrp: 1.501 ± 0.472
0.584SerTyr: 0.584 ± 0.248
0.0SerXaa: 0.0 ± 0.0
Thr
9.006ThrAla: 9.006 ± 1.21
0.584ThrCys: 0.584 ± 0.292
5.837ThrAsp: 5.837 ± 0.538
3.169ThrGlu: 3.169 ± 0.47
1.501ThrPhe: 1.501 ± 0.366
6.171ThrGly: 6.171 ± 0.694
1.167ThrHis: 1.167 ± 0.3
3.669ThrIle: 3.669 ± 0.535
2.668ThrLys: 2.668 ± 0.466
6.421ThrLeu: 6.421 ± 0.894
1.418ThrMet: 1.418 ± 0.333
1.334ThrAsn: 1.334 ± 0.302
4.837ThrPro: 4.837 ± 0.646
2.668ThrGln: 2.668 ± 0.493
3.919ThrArg: 3.919 ± 0.459
3.919ThrSer: 3.919 ± 0.863
4.753ThrThr: 4.753 ± 0.991
6.421ThrVal: 6.421 ± 0.796
1.918ThrTrp: 1.918 ± 0.328
0.917ThrTyr: 0.917 ± 0.286
0.0ThrXaa: 0.0 ± 0.0
Val
9.673ValAla: 9.673 ± 0.745
1.001ValCys: 1.001 ± 0.363
5.087ValAsp: 5.087 ± 0.652
4.086ValGlu: 4.086 ± 0.605
2.001ValPhe: 2.001 ± 0.559
5.003ValGly: 5.003 ± 0.726
1.001ValHis: 1.001 ± 0.291
3.586ValIle: 3.586 ± 0.631
3.753ValLys: 3.753 ± 0.614
5.003ValLeu: 5.003 ± 0.623
2.085ValMet: 2.085 ± 0.319
1.751ValAsn: 1.751 ± 0.505
4.503ValPro: 4.503 ± 0.738
2.835ValGln: 2.835 ± 0.568
5.003ValArg: 5.003 ± 0.55
5.003ValSer: 5.003 ± 0.673
5.504ValThr: 5.504 ± 0.669
5.254ValVal: 5.254 ± 0.959
2.252ValTrp: 2.252 ± 0.465
0.834ValTyr: 0.834 ± 0.277
0.0ValXaa: 0.0 ± 0.0
Trp
2.085TrpAla: 2.085 ± 0.395
0.334TrpCys: 0.334 ± 0.151
1.501TrpAsp: 1.501 ± 0.396
1.334TrpGlu: 1.334 ± 0.364
0.917TrpPhe: 0.917 ± 0.364
1.668TrpGly: 1.668 ± 0.357
0.417TrpHis: 0.417 ± 0.215
1.334TrpIle: 1.334 ± 0.401
0.751TrpLys: 0.751 ± 0.203
2.668TrpLeu: 2.668 ± 0.554
0.834TrpMet: 0.834 ± 0.266
1.001TrpAsn: 1.001 ± 0.371
1.001TrpPro: 1.001 ± 0.295
1.584TrpGln: 1.584 ± 0.331
2.835TrpArg: 2.835 ± 0.522
1.251TrpSer: 1.251 ± 0.405
2.418TrpThr: 2.418 ± 0.519
1.918TrpVal: 1.918 ± 0.401
0.667TrpTrp: 0.667 ± 0.232
0.25TrpTyr: 0.25 ± 0.143
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.751TyrAla: 1.751 ± 0.388
0.167TyrCys: 0.167 ± 0.114
1.001TyrAsp: 1.001 ± 0.287
0.334TyrGlu: 0.334 ± 0.184
0.584TyrPhe: 0.584 ± 0.187
0.917TyrGly: 0.917 ± 0.259
0.25TyrHis: 0.25 ± 0.126
0.417TyrIle: 0.417 ± 0.169
0.334TyrLys: 0.334 ± 0.164
1.001TyrLeu: 1.001 ± 0.256
0.0TyrMet: 0.0 ± 0.0
0.25TyrAsn: 0.25 ± 0.133
0.667TyrPro: 0.667 ± 0.284
0.584TyrGln: 0.584 ± 0.202
1.167TyrArg: 1.167 ± 0.307
1.001TyrSer: 1.001 ± 0.352
0.917TyrThr: 0.917 ± 0.314
0.584TyrVal: 0.584 ± 0.228
0.417TyrTrp: 0.417 ± 0.172
0.167TyrTyr: 0.167 ± 0.108
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (11993 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski