Amino acid dipepetide frequency for Propionibacterium phage PA6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.266AlaAla: 10.266 ± 1.555
1.339AlaCys: 1.339 ± 0.358
6.137AlaAsp: 6.137 ± 0.982
5.691AlaGlu: 5.691 ± 0.955
2.566AlaPhe: 2.566 ± 0.563
9.931AlaGly: 9.931 ± 1.237
2.455AlaHis: 2.455 ± 0.508
3.571AlaIle: 3.571 ± 0.619
4.352AlaLys: 4.352 ± 0.74
7.141AlaLeu: 7.141 ± 1.091
3.682AlaMet: 3.682 ± 1.167
2.566AlaAsn: 2.566 ± 0.497
2.901AlaPro: 2.901 ± 0.496
3.794AlaGln: 3.794 ± 0.64
6.137AlaArg: 6.137 ± 0.899
6.472AlaSer: 6.472 ± 0.805
6.695AlaThr: 6.695 ± 0.999
8.815AlaVal: 8.815 ± 1.246
1.562AlaTrp: 1.562 ± 0.443
2.455AlaTyr: 2.455 ± 0.432
0.0AlaXaa: 0.0 ± 0.0
Cys
1.227CysAla: 1.227 ± 0.369
0.446CysCys: 0.446 ± 0.299
1.116CysAsp: 1.116 ± 0.392
1.116CysGlu: 1.116 ± 0.345
0.112CysPhe: 0.112 ± 0.1
1.562CysGly: 1.562 ± 0.397
0.669CysHis: 0.669 ± 0.319
0.558CysIle: 0.558 ± 0.216
0.558CysLys: 0.558 ± 0.28
0.335CysLeu: 0.335 ± 0.182
0.223CysMet: 0.223 ± 0.171
0.335CysAsn: 0.335 ± 0.211
1.116CysPro: 1.116 ± 0.358
0.669CysGln: 0.669 ± 0.261
1.339CysArg: 1.339 ± 0.405
0.558CysSer: 0.558 ± 0.297
0.893CysThr: 0.893 ± 0.289
0.781CysVal: 0.781 ± 0.279
0.335CysTrp: 0.335 ± 0.21
0.223CysTyr: 0.223 ± 0.144
0.0CysXaa: 0.0 ± 0.0
Asp
4.91AspAla: 4.91 ± 0.726
0.781AspCys: 0.781 ± 0.259
4.686AspAsp: 4.686 ± 0.672
3.682AspGlu: 3.682 ± 0.672
1.674AspPhe: 1.674 ± 0.348
6.137AspGly: 6.137 ± 1.558
2.008AspHis: 2.008 ± 0.507
2.678AspIle: 2.678 ± 0.757
2.008AspLys: 2.008 ± 0.453
4.463AspLeu: 4.463 ± 0.828
1.339AspMet: 1.339 ± 0.347
2.566AspAsn: 2.566 ± 0.654
3.459AspPro: 3.459 ± 0.878
1.339AspGln: 1.339 ± 0.434
4.017AspArg: 4.017 ± 0.643
3.571AspSer: 3.571 ± 0.491
4.686AspThr: 4.686 ± 0.738
4.575AspVal: 4.575 ± 0.719
1.562AspTrp: 1.562 ± 0.481
2.12AspTyr: 2.12 ± 0.614
0.0AspXaa: 0.0 ± 0.0
Glu
5.468GluAla: 5.468 ± 0.976
0.558GluCys: 0.558 ± 0.221
2.008GluAsp: 2.008 ± 0.567
1.562GluGlu: 1.562 ± 0.474
1.339GluPhe: 1.339 ± 0.337
3.459GluGly: 3.459 ± 0.601
0.781GluHis: 0.781 ± 0.312
2.008GluIle: 2.008 ± 0.408
1.562GluLys: 1.562 ± 0.448
3.347GluLeu: 3.347 ± 0.609
1.227GluMet: 1.227 ± 0.309
1.897GluAsn: 1.897 ± 0.44
1.451GluPro: 1.451 ± 0.414
2.455GluGln: 2.455 ± 0.478
3.571GluArg: 3.571 ± 0.84
4.24GluSer: 4.24 ± 0.816
3.124GluThr: 3.124 ± 0.637
3.347GluVal: 3.347 ± 0.62
1.897GluTrp: 1.897 ± 0.562
1.674GluTyr: 1.674 ± 0.537
0.0GluXaa: 0.0 ± 0.0
Phe
2.79PheAla: 2.79 ± 0.799
0.558PheCys: 0.558 ± 0.249
2.008PheAsp: 2.008 ± 0.613
1.339PheGlu: 1.339 ± 0.337
1.116PhePhe: 1.116 ± 0.33
2.455PheGly: 2.455 ± 0.589
0.558PheHis: 0.558 ± 0.218
1.227PheIle: 1.227 ± 0.317
1.116PheLys: 1.116 ± 0.346
2.008PheLeu: 2.008 ± 0.476
0.893PheMet: 0.893 ± 0.274
1.004PheAsn: 1.004 ± 0.367
1.451PhePro: 1.451 ± 0.365
0.781PheGln: 0.781 ± 0.243
1.897PheArg: 1.897 ± 0.47
2.12PheSer: 2.12 ± 0.524
1.897PheThr: 1.897 ± 0.429
1.785PheVal: 1.785 ± 0.501
0.558PheTrp: 0.558 ± 0.218
0.223PheTyr: 0.223 ± 0.146
0.0PheXaa: 0.0 ± 0.0
Gly
7.141GlyAla: 7.141 ± 1.384
0.781GlyCys: 0.781 ± 0.337
6.025GlyAsp: 6.025 ± 0.717
4.575GlyGlu: 4.575 ± 0.653
2.901GlyPhe: 2.901 ± 0.697
6.583GlyGly: 6.583 ± 0.882
1.562GlyHis: 1.562 ± 0.419
2.901GlyIle: 2.901 ± 0.704
5.133GlyLys: 5.133 ± 0.663
7.699GlyLeu: 7.699 ± 1.187
2.12GlyMet: 2.12 ± 0.543
2.232GlyAsn: 2.232 ± 0.481
4.129GlyPro: 4.129 ± 1.088
3.013GlyGln: 3.013 ± 0.564
4.91GlyArg: 4.91 ± 0.96
6.36GlySer: 6.36 ± 0.941
4.575GlyThr: 4.575 ± 0.73
8.703GlyVal: 8.703 ± 1.276
1.785GlyTrp: 1.785 ± 0.433
3.236GlyTyr: 3.236 ± 0.589
0.0GlyXaa: 0.0 ± 0.0
His
2.008HisAla: 2.008 ± 0.628
0.893HisCys: 0.893 ± 0.275
1.227HisAsp: 1.227 ± 0.459
0.558HisGlu: 0.558 ± 0.303
0.335HisPhe: 0.335 ± 0.244
1.897HisGly: 1.897 ± 0.385
1.451HisHis: 1.451 ± 0.433
1.451HisIle: 1.451 ± 0.428
1.674HisLys: 1.674 ± 0.437
3.124HisLeu: 3.124 ± 0.763
0.446HisMet: 0.446 ± 0.196
1.227HisAsn: 1.227 ± 0.404
1.339HisPro: 1.339 ± 0.434
1.339HisGln: 1.339 ± 0.439
1.227HisArg: 1.227 ± 0.356
1.785HisSer: 1.785 ± 0.452
2.12HisThr: 2.12 ± 0.628
1.562HisVal: 1.562 ± 0.476
0.335HisTrp: 0.335 ± 0.198
1.004HisTyr: 1.004 ± 0.352
0.0HisXaa: 0.0 ± 0.0
Ile
4.352IleAla: 4.352 ± 0.705
1.004IleCys: 1.004 ± 0.337
4.575IleAsp: 4.575 ± 0.514
2.901IleGlu: 2.901 ± 0.48
1.227IlePhe: 1.227 ± 0.278
2.343IleGly: 2.343 ± 0.718
1.339IleHis: 1.339 ± 0.403
2.678IleIle: 2.678 ± 0.7
1.562IleLys: 1.562 ± 0.378
2.79IleLeu: 2.79 ± 0.516
1.451IleMet: 1.451 ± 0.395
1.897IleAsn: 1.897 ± 0.446
3.013IlePro: 3.013 ± 0.48
1.674IleGln: 1.674 ± 0.373
2.678IleArg: 2.678 ± 0.514
2.455IleSer: 2.455 ± 0.442
3.794IleThr: 3.794 ± 0.821
3.236IleVal: 3.236 ± 0.524
0.669IleTrp: 0.669 ± 0.29
1.227IleTyr: 1.227 ± 0.411
0.0IleXaa: 0.0 ± 0.0
Lys
4.352LysAla: 4.352 ± 0.873
0.335LysCys: 0.335 ± 0.188
2.566LysAsp: 2.566 ± 0.699
1.004LysGlu: 1.004 ± 0.323
0.781LysPhe: 0.781 ± 0.281
4.017LysGly: 4.017 ± 0.646
1.227LysHis: 1.227 ± 0.457
1.339LysIle: 1.339 ± 0.365
1.451LysLys: 1.451 ± 0.415
3.459LysLeu: 3.459 ± 0.547
0.669LysMet: 0.669 ± 0.236
1.562LysAsn: 1.562 ± 0.339
3.124LysPro: 3.124 ± 0.575
2.232LysGln: 2.232 ± 0.676
2.343LysArg: 2.343 ± 0.583
2.008LysSer: 2.008 ± 0.643
4.017LysThr: 4.017 ± 0.81
1.674LysVal: 1.674 ± 0.397
0.669LysTrp: 0.669 ± 0.244
1.227LysTyr: 1.227 ± 0.387
0.0LysXaa: 0.0 ± 0.0
Leu
9.373LeuAla: 9.373 ± 1.038
1.339LeuCys: 1.339 ± 0.385
4.686LeuAsp: 4.686 ± 0.853
3.236LeuGlu: 3.236 ± 0.628
1.897LeuPhe: 1.897 ± 0.484
6.36LeuGly: 6.36 ± 1.142
2.455LeuHis: 2.455 ± 0.524
3.124LeuIle: 3.124 ± 0.631
4.017LeuLys: 4.017 ± 0.737
5.133LeuLeu: 5.133 ± 0.802
1.562LeuMet: 1.562 ± 0.352
2.455LeuAsn: 2.455 ± 0.446
4.686LeuPro: 4.686 ± 0.571
3.013LeuGln: 3.013 ± 0.532
3.571LeuArg: 3.571 ± 0.594
5.802LeuSer: 5.802 ± 0.75
5.021LeuThr: 5.021 ± 0.824
3.682LeuVal: 3.682 ± 0.613
0.781LeuTrp: 0.781 ± 0.37
1.451LeuTyr: 1.451 ± 0.503
0.0LeuXaa: 0.0 ± 0.0
Met
3.236MetAla: 3.236 ± 0.516
0.669MetCys: 0.669 ± 0.238
1.004MetAsp: 1.004 ± 0.31
0.893MetGlu: 0.893 ± 0.341
1.227MetPhe: 1.227 ± 0.336
1.674MetGly: 1.674 ± 0.597
0.669MetHis: 0.669 ± 0.23
2.008MetIle: 2.008 ± 0.451
0.669MetLys: 0.669 ± 0.296
2.343MetLeu: 2.343 ± 0.378
0.446MetMet: 0.446 ± 0.21
1.004MetAsn: 1.004 ± 0.316
1.674MetPro: 1.674 ± 0.587
0.893MetGln: 0.893 ± 0.287
1.116MetArg: 1.116 ± 0.337
1.897MetSer: 1.897 ± 0.426
1.004MetThr: 1.004 ± 0.309
1.562MetVal: 1.562 ± 0.383
0.669MetTrp: 0.669 ± 0.254
1.116MetTyr: 1.116 ± 0.369
0.0MetXaa: 0.0 ± 0.0
Asn
2.678AsnAla: 2.678 ± 0.672
0.223AsnCys: 0.223 ± 0.165
1.339AsnAsp: 1.339 ± 0.318
1.339AsnGlu: 1.339 ± 0.325
0.558AsnPhe: 0.558 ± 0.214
5.356AsnGly: 5.356 ± 0.929
1.339AsnHis: 1.339 ± 0.388
1.785AsnIle: 1.785 ± 0.428
1.674AsnLys: 1.674 ± 0.496
1.562AsnLeu: 1.562 ± 0.438
1.004AsnMet: 1.004 ± 0.275
2.343AsnAsn: 2.343 ± 0.654
3.124AsnPro: 3.124 ± 0.656
1.451AsnGln: 1.451 ± 0.362
2.343AsnArg: 2.343 ± 0.61
1.674AsnSer: 1.674 ± 0.465
2.455AsnThr: 2.455 ± 0.629
2.343AsnVal: 2.343 ± 0.459
0.446AsnTrp: 0.446 ± 0.216
0.781AsnTyr: 0.781 ± 0.271
0.0AsnXaa: 0.0 ± 0.0
Pro
6.025ProAla: 6.025 ± 0.986
0.781ProCys: 0.781 ± 0.291
4.24ProAsp: 4.24 ± 0.822
3.013ProGlu: 3.013 ± 0.566
1.227ProPhe: 1.227 ± 0.393
4.91ProGly: 4.91 ± 0.963
1.562ProHis: 1.562 ± 0.444
1.785ProIle: 1.785 ± 0.392
1.674ProLys: 1.674 ± 0.378
3.347ProLeu: 3.347 ± 0.548
0.781ProMet: 0.781 ± 0.276
2.566ProAsn: 2.566 ± 0.532
3.459ProPro: 3.459 ± 0.678
1.897ProGln: 1.897 ± 0.594
1.785ProArg: 1.785 ± 0.509
4.017ProSer: 4.017 ± 0.663
2.343ProThr: 2.343 ± 0.443
5.133ProVal: 5.133 ± 1.233
1.785ProTrp: 1.785 ± 0.475
1.339ProTyr: 1.339 ± 0.361
0.0ProXaa: 0.0 ± 0.0
Gln
3.905GlnAla: 3.905 ± 1.015
0.446GlnCys: 0.446 ± 0.238
1.562GlnAsp: 1.562 ± 0.405
1.227GlnGlu: 1.227 ± 0.381
0.781GlnPhe: 0.781 ± 0.242
2.566GlnGly: 2.566 ± 0.753
1.562GlnHis: 1.562 ± 0.574
2.79GlnIle: 2.79 ± 0.594
1.004GlnLys: 1.004 ± 0.303
3.347GlnLeu: 3.347 ± 0.558
1.004GlnMet: 1.004 ± 0.339
0.781GlnAsn: 0.781 ± 0.232
2.455GlnPro: 2.455 ± 0.57
3.013GlnGln: 3.013 ± 0.684
2.79GlnArg: 2.79 ± 0.516
2.343GlnSer: 2.343 ± 0.483
3.013GlnThr: 3.013 ± 0.643
3.124GlnVal: 3.124 ± 0.605
0.893GlnTrp: 0.893 ± 0.35
1.116GlnTyr: 1.116 ± 0.358
0.0GlnXaa: 0.0 ± 0.0
Arg
5.914ArgAla: 5.914 ± 0.863
0.558ArgCys: 0.558 ± 0.254
3.459ArgAsp: 3.459 ± 0.743
2.678ArgGlu: 2.678 ± 0.554
2.008ArgPhe: 2.008 ± 0.595
4.575ArgGly: 4.575 ± 0.799
1.451ArgHis: 1.451 ± 0.427
3.013ArgIle: 3.013 ± 0.632
2.343ArgLys: 2.343 ± 0.584
6.36ArgLeu: 6.36 ± 0.846
1.897ArgMet: 1.897 ± 0.451
2.232ArgAsn: 2.232 ± 0.593
2.008ArgPro: 2.008 ± 0.447
2.79ArgGln: 2.79 ± 0.513
5.802ArgArg: 5.802 ± 1.116
4.91ArgSer: 4.91 ± 0.763
3.124ArgThr: 3.124 ± 0.681
5.244ArgVal: 5.244 ± 0.721
1.116ArgTrp: 1.116 ± 0.362
1.785ArgTyr: 1.785 ± 0.422
0.0ArgXaa: 0.0 ± 0.0
Ser
7.588SerAla: 7.588 ± 1.143
0.558SerCys: 0.558 ± 0.25
3.571SerAsp: 3.571 ± 0.598
2.79SerGlu: 2.79 ± 0.504
2.79SerPhe: 2.79 ± 0.593
8.369SerGly: 8.369 ± 1.014
1.785SerHis: 1.785 ± 0.531
3.347SerIle: 3.347 ± 0.46
1.785SerLys: 1.785 ± 0.576
4.686SerLeu: 4.686 ± 0.567
2.343SerMet: 2.343 ± 0.378
2.232SerAsn: 2.232 ± 0.474
3.347SerPro: 3.347 ± 0.685
2.008SerGln: 2.008 ± 0.383
4.24SerArg: 4.24 ± 0.92
4.91SerSer: 4.91 ± 0.854
4.352SerThr: 4.352 ± 0.909
5.691SerVal: 5.691 ± 0.902
1.674SerTrp: 1.674 ± 0.379
0.893SerTyr: 0.893 ± 0.299
0.0SerXaa: 0.0 ± 0.0
Thr
6.807ThrAla: 6.807 ± 1.055
1.116ThrCys: 1.116 ± 0.314
3.459ThrAsp: 3.459 ± 0.579
2.008ThrGlu: 2.008 ± 0.481
2.232ThrPhe: 2.232 ± 0.487
4.91ThrGly: 4.91 ± 0.638
1.562ThrHis: 1.562 ± 0.447
5.133ThrIle: 5.133 ± 0.776
2.566ThrLys: 2.566 ± 0.555
3.905ThrLeu: 3.905 ± 0.708
1.227ThrMet: 1.227 ± 0.33
1.785ThrAsn: 1.785 ± 0.457
5.021ThrPro: 5.021 ± 0.731
3.013ThrGln: 3.013 ± 0.631
3.347ThrArg: 3.347 ± 0.602
4.798ThrSer: 4.798 ± 0.763
4.686ThrThr: 4.686 ± 1.098
5.468ThrVal: 5.468 ± 0.776
1.004ThrTrp: 1.004 ± 0.36
1.897ThrTyr: 1.897 ± 0.662
0.0ThrXaa: 0.0 ± 0.0
Val
6.807ValAla: 6.807 ± 1.413
1.116ValCys: 1.116 ± 0.401
5.244ValAsp: 5.244 ± 0.835
5.133ValGlu: 5.133 ± 0.753
2.12ValPhe: 2.12 ± 0.538
6.025ValGly: 6.025 ± 1.067
1.227ValHis: 1.227 ± 0.305
3.124ValIle: 3.124 ± 0.823
3.459ValLys: 3.459 ± 0.704
6.025ValLeu: 6.025 ± 0.947
2.343ValMet: 2.343 ± 0.622
2.79ValAsn: 2.79 ± 0.549
3.236ValPro: 3.236 ± 0.558
2.678ValGln: 2.678 ± 0.599
4.91ValArg: 4.91 ± 0.707
6.583ValSer: 6.583 ± 0.849
4.129ValThr: 4.129 ± 0.613
6.249ValVal: 6.249 ± 1.141
1.339ValTrp: 1.339 ± 0.37
1.339ValTyr: 1.339 ± 0.389
0.0ValXaa: 0.0 ± 0.0
Trp
1.562TrpAla: 1.562 ± 0.392
0.335TrpCys: 0.335 ± 0.175
0.781TrpAsp: 0.781 ± 0.262
1.116TrpGlu: 1.116 ± 0.308
0.335TrpPhe: 0.335 ± 0.176
1.116TrpGly: 1.116 ± 0.498
0.558TrpHis: 0.558 ± 0.286
1.004TrpIle: 1.004 ± 0.297
0.669TrpLys: 0.669 ± 0.304
1.451TrpLeu: 1.451 ± 0.485
0.669TrpMet: 0.669 ± 0.279
1.004TrpAsn: 1.004 ± 0.336
0.669TrpPro: 0.669 ± 0.232
0.781TrpGln: 0.781 ± 0.297
2.566TrpArg: 2.566 ± 0.67
1.339TrpSer: 1.339 ± 0.432
1.785TrpThr: 1.785 ± 0.391
0.781TrpVal: 0.781 ± 0.372
0.558TrpTrp: 0.558 ± 0.242
1.004TrpTyr: 1.004 ± 0.369
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.12TyrAla: 2.12 ± 0.426
0.335TyrCys: 0.335 ± 0.182
2.566TyrAsp: 2.566 ± 0.422
1.116TyrGlu: 1.116 ± 0.335
0.558TyrPhe: 0.558 ± 0.201
2.12TyrGly: 2.12 ± 0.388
0.781TyrHis: 0.781 ± 0.235
1.339TyrIle: 1.339 ± 0.344
0.781TyrLys: 0.781 ± 0.302
1.116TyrLeu: 1.116 ± 0.288
0.335TyrMet: 0.335 ± 0.182
1.339TyrAsn: 1.339 ± 0.487
2.008TyrPro: 2.008 ± 0.584
0.893TyrGln: 0.893 ± 0.353
2.678TyrArg: 2.678 ± 0.493
1.116TyrSer: 1.116 ± 0.355
2.232TyrThr: 2.232 ± 0.422
2.12TyrVal: 2.12 ± 0.474
0.558TyrTrp: 0.558 ± 0.317
1.116TyrTyr: 1.116 ± 0.401
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (8963 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski