Amino acid dipepetide frequency for Rubrobacter radiotolerans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.102AlaAla: 14.102 ± 0.172	0.85AlaCys: 0.85 ± 0.032	4.975AlaAsp: 4.975 ± 0.076	8.291AlaGlu: 8.291 ± 0.108	3.929AlaPhe: 3.929 ± 0.074	11.584AlaGly: 11.584 ± 0.123	1.672AlaHis: 1.672 ± 0.047	3.979AlaIle: 3.979 ± 0.085	2.733AlaLys: 2.733 ± 0.064	13.174AlaLeu: 13.174 ± 0.155	2.259AlaMet: 2.259 ± 0.044	2.2AlaAsn: 2.2 ± 0.05	4.729AlaPro: 4.729 ± 0.069	2.402AlaGln: 2.402 ± 0.066	9.367AlaArg: 9.367 ± 0.116	6.413AlaSer: 6.413 ± 0.086	5.248AlaThr: 5.248 ± 0.081	9.863AlaVal: 9.863 ± 0.115	1.093AlaTrp: 1.093 ± 0.034	2.489AlaTyr: 2.489 ± 0.055	0.0AlaXaa: 0.0 ± 0.0
Cys	0.807CysAla: 0.807 ± 0.033	0.099CysCys: 0.099 ± 0.01	0.362CysAsp: 0.362 ± 0.02	0.534CysGlu: 0.534 ± 0.026	0.229CysPhe: 0.229 ± 0.015	0.951CysGly: 0.951 ± 0.032	0.148CysHis: 0.148 ± 0.011	0.235CysIle: 0.235 ± 0.016	0.182CysLys: 0.182 ± 0.015	0.624CysLeu: 0.624 ± 0.027	0.098CysMet: 0.098 ± 0.01	0.15CysAsn: 0.15 ± 0.011	0.424CysPro: 0.424 ± 0.025	0.13CysGln: 0.13 ± 0.012	0.544CysArg: 0.544 ± 0.025	0.409CysSer: 0.409 ± 0.02	0.312CysThr: 0.312 ± 0.018	0.632CysVal: 0.632 ± 0.025	0.068CysTrp: 0.068 ± 0.009	0.165CysTyr: 0.165 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	5.747AspAla: 5.747 ± 0.077	0.276AspCys: 0.276 ± 0.016	2.431AspAsp: 2.431 ± 0.06	4.35AspGlu: 4.35 ± 0.079	2.062AspPhe: 2.062 ± 0.041	5.191AspGly: 5.191 ± 0.081	0.921AspHis: 0.921 ± 0.032	1.766AspIle: 1.766 ± 0.048	0.929AspLys: 0.929 ± 0.033	6.723AspLeu: 6.723 ± 0.072	0.716AspMet: 0.716 ± 0.03	0.89AspAsn: 0.89 ± 0.03	3.488AspPro: 3.488 ± 0.066	0.878AspGln: 0.878 ± 0.037	4.348AspArg: 4.348 ± 0.072	2.287AspSer: 2.287 ± 0.049	2.15AspThr: 2.15 ± 0.052	4.321AspVal: 4.321 ± 0.071	0.65AspTrp: 0.65 ± 0.028	1.495AspTyr: 1.495 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	9.819GluAla: 9.819 ± 0.129	0.407GluCys: 0.407 ± 0.024	4.112GluAsp: 4.112 ± 0.065	7.598GluGlu: 7.598 ± 0.11	2.329GluPhe: 2.329 ± 0.049	7.2GluGly: 7.2 ± 0.092	1.457GluHis: 1.457 ± 0.042	3.469GluIle: 3.469 ± 0.068	2.819GluLys: 2.819 ± 0.066	6.983GluLeu: 6.983 ± 0.091	1.521GluMet: 1.521 ± 0.046	2.107GluAsn: 2.107 ± 0.051	3.974GluPro: 3.974 ± 0.07	1.963GluGln: 1.963 ± 0.054	9.004GluArg: 9.004 ± 0.122	4.368GluSer: 4.368 ± 0.073	3.943GluThr: 3.943 ± 0.075	7.485GluVal: 7.485 ± 0.098	0.86GluTrp: 0.86 ± 0.03	1.706GluTyr: 1.706 ± 0.044	0.0GluXaa: 0.0 ± 0.0
Phe	4.003PheAla: 4.003 ± 0.071	0.339PheCys: 0.339 ± 0.021	2.06PheAsp: 2.06 ± 0.053	2.655PheGlu: 2.655 ± 0.054	1.62PhePhe: 1.62 ± 0.048	4.195PheGly: 4.195 ± 0.077	0.587PheHis: 0.587 ± 0.024	1.294PheIle: 1.294 ± 0.04	0.713PheLys: 0.713 ± 0.027	3.603PheLeu: 3.603 ± 0.081	0.637PheMet: 0.637 ± 0.028	0.757PheAsn: 0.757 ± 0.032	1.626PhePro: 1.626 ± 0.04	0.716PheGln: 0.716 ± 0.03	2.453PheArg: 2.453 ± 0.062	2.313PheSer: 2.313 ± 0.052	1.85PheThr: 1.85 ± 0.052	3.312PheVal: 3.312 ± 0.071	0.511PheTrp: 0.511 ± 0.025	1.071PheTyr: 1.071 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	10.343GlyAla: 10.343 ± 0.107	0.789GlyCys: 0.789 ± 0.033	4.674GlyAsp: 4.674 ± 0.076	8.481GlyGlu: 8.481 ± 0.121	3.787GlyPhe: 3.787 ± 0.067	9.57GlyGly: 9.57 ± 0.14	1.575GlyHis: 1.575 ± 0.043	4.077GlyIle: 4.077 ± 0.071	2.767GlyLys: 2.767 ± 0.059	9.63GlyLeu: 9.63 ± 0.122	2.289GlyMet: 2.289 ± 0.046	1.952GlyAsn: 1.952 ± 0.053	4.077GlyPro: 4.077 ± 0.063	2.03GlyGln: 2.03 ± 0.064	7.991GlyArg: 7.991 ± 0.098	5.839GlySer: 5.839 ± 0.089	5.26GlyThr: 5.26 ± 0.076	8.187GlyVal: 8.187 ± 0.103	1.332GlyTrp: 1.332 ± 0.043	2.743GlyTyr: 2.743 ± 0.059	0.0GlyXaa: 0.0 ± 0.0
His	1.824HisAla: 1.824 ± 0.049	0.156HisCys: 0.156 ± 0.011	0.964HisAsp: 0.964 ± 0.034	1.291HisGlu: 1.291 ± 0.038	0.607HisPhe: 0.607 ± 0.022	1.49HisGly: 1.49 ± 0.038	0.448HisHis: 0.448 ± 0.023	0.614HisIle: 0.614 ± 0.025	0.348HisLys: 0.348 ± 0.021	1.849HisLeu: 1.849 ± 0.051	0.254HisMet: 0.254 ± 0.016	0.373HisAsn: 0.373 ± 0.019	1.217HisPro: 1.217 ± 0.036	0.319HisGln: 0.319 ± 0.019	1.355HisArg: 1.355 ± 0.038	0.827HisSer: 0.827 ± 0.031	0.77HisThr: 0.77 ± 0.026	1.323HisVal: 1.323 ± 0.037	0.181HisTrp: 0.181 ± 0.014	0.513HisTyr: 0.513 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	3.948IleAla: 3.948 ± 0.077	0.295IleCys: 0.295 ± 0.019	1.801IleAsp: 1.801 ± 0.048	3.191IleGlu: 3.191 ± 0.061	1.392IlePhe: 1.392 ± 0.041	3.631IleGly: 3.631 ± 0.079	0.674IleHis: 0.674 ± 0.025	1.388IleIle: 1.388 ± 0.048	1.059IleLys: 1.059 ± 0.033	3.772IleLeu: 3.772 ± 0.065	0.602IleMet: 0.602 ± 0.025	0.914IleAsn: 0.914 ± 0.036	2.11IlePro: 2.11 ± 0.049	0.827IleGln: 0.827 ± 0.031	2.585IleArg: 2.585 ± 0.05	2.817IleSer: 2.817 ± 0.056	2.084IleThr: 2.084 ± 0.056	3.558IleVal: 3.558 ± 0.076	0.287IleTrp: 0.287 ± 0.018	0.84IleTyr: 0.84 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	2.479LysAla: 2.479 ± 0.061	0.113LysCys: 0.113 ± 0.011	1.554LysAsp: 1.554 ± 0.038	2.013LysGlu: 2.013 ± 0.046	0.624LysPhe: 0.624 ± 0.024	2.144LysGly: 2.144 ± 0.05	0.461LysHis: 0.461 ± 0.024	1.044LysIle: 1.044 ± 0.038	1.165LysLys: 1.165 ± 0.042	2.704LysLeu: 2.704 ± 0.052	0.577LysMet: 0.577 ± 0.028	0.873LysAsn: 0.873 ± 0.032	1.351LysPro: 1.351 ± 0.041	0.619LysGln: 0.619 ± 0.032	2.323LysArg: 2.323 ± 0.055	1.567LysSer: 1.567 ± 0.04	1.67LysThr: 1.67 ± 0.047	2.502LysVal: 2.502 ± 0.053	0.214LysTrp: 0.214 ± 0.014	0.567LysTyr: 0.567 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	13.727LeuAla: 13.727 ± 0.156	0.819LeuCys: 0.819 ± 0.03	6.033LeuAsp: 6.033 ± 0.095	6.942LeuGlu: 6.942 ± 0.085	3.821LeuPhe: 3.821 ± 0.079	10.141LeuGly: 10.141 ± 0.131	1.568LeuHis: 1.568 ± 0.043	3.726LeuIle: 3.726 ± 0.074	2.824LeuLys: 2.824 ± 0.051	11.008LeuLeu: 11.008 ± 0.159	1.757LeuMet: 1.757 ± 0.041	1.919LeuAsn: 1.919 ± 0.041	5.635LeuPro: 5.635 ± 0.08	2.077LeuGln: 2.077 ± 0.046	8.148LeuArg: 8.148 ± 0.105	6.605LeuSer: 6.605 ± 0.081	4.874LeuThr: 4.874 ± 0.067	9.036LeuVal: 9.036 ± 0.111	1.132LeuTrp: 1.132 ± 0.035	2.622LeuTyr: 2.622 ± 0.05	0.0LeuXaa: 0.0 ± 0.0
Met	1.803MetAla: 1.803 ± 0.041	0.148MetCys: 0.148 ± 0.013	0.978MetAsp: 0.978 ± 0.035	1.36MetGlu: 1.36 ± 0.039	0.562MetPhe: 0.562 ± 0.025	1.524MetGly: 1.524 ± 0.045	0.347MetHis: 0.347 ± 0.02	0.898MetIle: 0.898 ± 0.029	0.784MetLys: 0.784 ± 0.03	1.932MetLeu: 1.932 ± 0.04	0.399MetMet: 0.399 ± 0.026	0.589MetAsn: 0.589 ± 0.025	0.986MetPro: 0.986 ± 0.033	0.591MetGln: 0.591 ± 0.028	1.637MetArg: 1.637 ± 0.046	1.504MetSer: 1.504 ± 0.043	1.168MetThr: 1.168 ± 0.039	1.393MetVal: 1.393 ± 0.039	0.139MetTrp: 0.139 ± 0.013	0.378MetTyr: 0.378 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	2.407AsnAla: 2.407 ± 0.05	0.191AsnCys: 0.191 ± 0.015	1.088AsnAsp: 1.088 ± 0.04	1.359AsnGlu: 1.359 ± 0.042	0.764AsnPhe: 0.764 ± 0.028	2.034AsnGly: 2.034 ± 0.05	0.387AsnHis: 0.387 ± 0.02	0.963AsnIle: 0.963 ± 0.028	0.429AsnLys: 0.429 ± 0.02	2.532AsnLeu: 2.532 ± 0.051	0.356AsnMet: 0.356 ± 0.02	0.546AsnAsn: 0.546 ± 0.028	1.656AsnPro: 1.656 ± 0.043	0.491AsnGln: 0.491 ± 0.022	1.62AsnArg: 1.62 ± 0.041	1.087AsnSer: 1.087 ± 0.036	1.175AsnThr: 1.175 ± 0.034	2.098AsnVal: 2.098 ± 0.046	0.289AsnTrp: 0.289 ± 0.018	0.586AsnTyr: 0.586 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	5.08ProAla: 5.08 ± 0.074	0.267ProCys: 0.267 ± 0.019	3.622ProAsp: 3.622 ± 0.074	6.572ProGlu: 6.572 ± 0.095	1.791ProPhe: 1.791 ± 0.04	5.24ProGly: 5.24 ± 0.08	0.865ProHis: 0.865 ± 0.032	1.462ProIle: 1.462 ± 0.045	1.462ProLys: 1.462 ± 0.042	4.721ProLeu: 4.721 ± 0.064	0.87ProMet: 0.87 ± 0.028	1.259ProAsn: 1.259 ± 0.037	2.634ProPro: 2.634 ± 0.062	1.273ProGln: 1.273 ± 0.036	2.903ProArg: 2.903 ± 0.053	2.475ProSer: 2.475 ± 0.053	2.39ProThr: 2.39 ± 0.048	4.67ProVal: 4.67 ± 0.075	0.565ProTrp: 0.565 ± 0.025	1.289ProTyr: 1.289 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	2.604GlnAla: 2.604 ± 0.085	0.099GlnCys: 0.099 ± 0.009	1.109GlnAsp: 1.109 ± 0.039	1.773GlnGlu: 1.773 ± 0.048	0.663GlnPhe: 0.663 ± 0.026	1.807GlnGly: 1.807 ± 0.041	0.321GlnHis: 0.321 ± 0.015	0.977GlnIle: 0.977 ± 0.036	0.803GlnLys: 0.803 ± 0.032	1.848GlnLeu: 1.848 ± 0.049	0.561GlnMet: 0.561 ± 0.026	0.71GlnAsn: 0.71 ± 0.032	0.931GlnPro: 0.931 ± 0.028	0.867GlnGln: 0.867 ± 0.055	1.849GlnArg: 1.849 ± 0.04	1.265GlnSer: 1.265 ± 0.035	1.29GlnThr: 1.29 ± 0.043	1.779GlnVal: 1.779 ± 0.048	0.225GlnTrp: 0.225 ± 0.016	0.495GlnTyr: 0.495 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	8.146ArgAla: 8.146 ± 0.118	0.464ArgCys: 0.464 ± 0.026	4.501ArgAsp: 4.501 ± 0.073	8.833ArgGlu: 8.833 ± 0.123	2.957ArgPhe: 2.957 ± 0.055	6.173ArgGly: 6.173 ± 0.101	1.363ArgHis: 1.363 ± 0.035	3.361ArgIle: 3.361 ± 0.063	2.422ArgLys: 2.422 ± 0.049	8.514ArgLeu: 8.514 ± 0.126	1.892ArgMet: 1.892 ± 0.044	1.762ArgAsn: 1.762 ± 0.042	3.885ArgPro: 3.885 ± 0.067	1.932ArgGln: 1.932 ± 0.045	7.985ArgArg: 7.985 ± 0.127	4.812ArgSer: 4.812 ± 0.077	3.988ArgThr: 3.988 ± 0.067	6.303ArgVal: 6.303 ± 0.078	1.023ArgTrp: 1.023 ± 0.038	2.28ArgTyr: 2.28 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	5.767SerAla: 5.767 ± 0.08	0.417SerCys: 0.417 ± 0.022	2.88SerAsp: 2.88 ± 0.057	4.706SerGlu: 4.706 ± 0.073	2.28SerPhe: 2.28 ± 0.048	7.631SerGly: 7.631 ± 0.106	0.897SerHis: 0.897 ± 0.034	1.936SerIle: 1.936 ± 0.047	1.214SerLys: 1.214 ± 0.038	5.764SerLeu: 5.764 ± 0.09	1.146SerMet: 1.146 ± 0.035	1.1SerAsn: 1.1 ± 0.031	3.38SerPro: 3.38 ± 0.062	1.246SerGln: 1.246 ± 0.04	4.594SerArg: 4.594 ± 0.072	3.095SerSer: 3.095 ± 0.078	2.415SerThr: 2.415 ± 0.057	5.093SerVal: 5.093 ± 0.083	0.684SerTrp: 0.684 ± 0.026	1.447SerTyr: 1.447 ± 0.037	0.0SerXaa: 0.0 ± 0.0
Thr	4.875ThrAla: 4.875 ± 0.077	0.3ThrCys: 0.3 ± 0.018	2.684ThrAsp: 2.684 ± 0.051	3.433ThrGlu: 3.433 ± 0.066	1.926ThrPhe: 1.926 ± 0.048	5.31ThrGly: 5.31 ± 0.076	0.909ThrHis: 0.909 ± 0.032	1.822ThrIle: 1.822 ± 0.051	1.16ThrLys: 1.16 ± 0.04	5.764ThrLeu: 5.764 ± 0.088	0.909ThrMet: 0.909 ± 0.028	1.156ThrAsn: 1.156 ± 0.035	2.802ThrPro: 2.802 ± 0.063	1.075ThrGln: 1.075 ± 0.029	2.973ThrArg: 2.973 ± 0.053	2.738ThrSer: 2.738 ± 0.052	2.604ThrThr: 2.604 ± 0.066	4.934ThrVal: 4.934 ± 0.079	0.468ThrTrp: 0.468 ± 0.023	1.259ThrTyr: 1.259 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	10.103ValAla: 10.103 ± 0.113	0.812ValCys: 0.812 ± 0.032	3.905ValAsp: 3.905 ± 0.059	7.26ValGlu: 7.26 ± 0.084	3.612ValPhe: 3.612 ± 0.073	8.109ValGly: 8.109 ± 0.11	1.474ValHis: 1.474 ± 0.045	3.382ValIle: 3.382 ± 0.066	1.941ValLys: 1.941 ± 0.049	9.236ValLeu: 9.236 ± 0.116	1.605ValMet: 1.605 ± 0.042	1.857ValAsn: 1.857 ± 0.05	4.685ValPro: 4.685 ± 0.071	1.734ValGln: 1.734 ± 0.043	7.333ValArg: 7.333 ± 0.091	5.135ValSer: 5.135 ± 0.071	3.903ValThr: 3.903 ± 0.068	8.132ValVal: 8.132 ± 0.124	0.967ValTrp: 0.967 ± 0.037	2.209ValTyr: 2.209 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	1.037TrpAla: 1.037 ± 0.034	0.083TrpCys: 0.083 ± 0.011	0.546TrpAsp: 0.546 ± 0.028	0.712TrpGlu: 0.712 ± 0.032	0.412TrpPhe: 0.412 ± 0.023	0.893TrpGly: 0.893 ± 0.029	0.211TrpHis: 0.211 ± 0.015	0.521TrpIle: 0.521 ± 0.022	0.306TrpLys: 0.306 ± 0.021	1.213TrpLeu: 1.213 ± 0.034	0.291TrpMet: 0.291 ± 0.017	0.325TrpAsn: 0.325 ± 0.019	0.528TrpPro: 0.528 ± 0.024	0.321TrpGln: 0.321 ± 0.02	1.104TrpArg: 1.104 ± 0.033	0.749TrpSer: 0.749 ± 0.033	0.678TrpThr: 0.678 ± 0.027	0.774TrpVal: 0.774 ± 0.032	0.203TrpTrp: 0.203 ± 0.015	0.292TrpTyr: 0.292 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.733TyrAla: 2.733 ± 0.048	0.178TyrCys: 0.178 ± 0.013	1.34TyrAsp: 1.34 ± 0.039	1.771TyrGlu: 1.771 ± 0.039	0.947TyrPhe: 0.947 ± 0.035	2.574TyrGly: 2.574 ± 0.053	0.439TyrHis: 0.439 ± 0.023	0.86TyrIle: 0.86 ± 0.034	0.504TyrLys: 0.504 ± 0.025	2.821TyrLeu: 2.821 ± 0.066	0.38TyrMet: 0.38 ± 0.02	0.62TyrAsn: 0.62 ± 0.033	1.289TyrPro: 1.289 ± 0.036	0.488TyrGln: 0.488 ± 0.022	2.48TyrArg: 2.48 ± 0.057	1.39TyrSer: 1.39 ± 0.036	1.329TyrThr: 1.329 ± 0.043	1.999TyrVal: 1.999 ± 0.039	0.305TyrTrp: 0.305 ± 0.018	0.624TyrTyr: 0.624 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3148 proteins (976227 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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