Amino acid dipepetide frequency for Escherichia phage PGN829.1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.32AlaAla: 8.32 ± 1.162
0.765AlaCys: 0.765 ± 0.255
4.857AlaAsp: 4.857 ± 0.523
4.812AlaGlu: 4.812 ± 0.488
2.114AlaPhe: 2.114 ± 0.283
6.341AlaGly: 6.341 ± 0.685
1.079AlaHis: 1.079 ± 0.187
4.587AlaIle: 4.587 ± 0.605
5.622AlaLys: 5.622 ± 0.56
7.196AlaLeu: 7.196 ± 0.984
2.878AlaMet: 2.878 ± 0.498
5.532AlaAsn: 5.532 ± 0.429
2.474AlaPro: 2.474 ± 0.446
4.138AlaGln: 4.138 ± 0.895
3.463AlaArg: 3.463 ± 0.354
4.677AlaSer: 4.677 ± 0.474
6.072AlaThr: 6.072 ± 0.686
5.622AlaVal: 5.622 ± 0.476
0.989AlaTrp: 0.989 ± 0.218
3.598AlaTyr: 3.598 ± 0.402
0.0AlaXaa: 0.0 ± 0.0
Cys
0.585CysAla: 0.585 ± 0.202
0.045CysCys: 0.045 ± 0.041
0.495CysAsp: 0.495 ± 0.194
0.63CysGlu: 0.63 ± 0.205
0.36CysPhe: 0.36 ± 0.136
0.54CysGly: 0.54 ± 0.227
0.225CysHis: 0.225 ± 0.114
0.675CysIle: 0.675 ± 0.193
0.81CysLys: 0.81 ± 0.232
0.765CysLeu: 0.765 ± 0.287
0.315CysMet: 0.315 ± 0.129
0.585CysAsn: 0.585 ± 0.171
0.315CysPro: 0.315 ± 0.137
0.135CysGln: 0.135 ± 0.077
0.405CysArg: 0.405 ± 0.161
0.63CysSer: 0.63 ± 0.175
0.495CysThr: 0.495 ± 0.196
0.675CysVal: 0.675 ± 0.204
0.09CysTrp: 0.09 ± 0.066
0.36CysTyr: 0.36 ± 0.14
0.0CysXaa: 0.0 ± 0.0
Asp
4.857AspAla: 4.857 ± 0.512
0.989AspCys: 0.989 ± 0.293
3.643AspAsp: 3.643 ± 0.386
3.733AspGlu: 3.733 ± 0.542
2.384AspPhe: 2.384 ± 0.296
3.508AspGly: 3.508 ± 0.397
0.675AspHis: 0.675 ± 0.199
4.407AspIle: 4.407 ± 0.567
3.148AspLys: 3.148 ± 0.362
4.947AspLeu: 4.947 ± 0.618
1.619AspMet: 1.619 ± 0.265
2.653AspAsn: 2.653 ± 0.291
2.743AspPro: 2.743 ± 0.346
1.889AspGln: 1.889 ± 0.35
2.698AspArg: 2.698 ± 0.267
4.048AspSer: 4.048 ± 0.355
4.138AspThr: 4.138 ± 0.378
3.598AspVal: 3.598 ± 0.361
0.899AspTrp: 0.899 ± 0.177
2.339AspTyr: 2.339 ± 0.435
0.0AspXaa: 0.0 ± 0.0
Glu
7.061GluAla: 7.061 ± 0.895
0.405GluCys: 0.405 ± 0.192
3.868GluAsp: 3.868 ± 0.547
5.937GluGlu: 5.937 ± 0.733
2.249GluPhe: 2.249 ± 0.305
4.273GluGly: 4.273 ± 0.531
0.944GluHis: 0.944 ± 0.204
3.103GluIle: 3.103 ± 0.377
3.778GluLys: 3.778 ± 0.486
5.487GluLeu: 5.487 ± 0.439
2.069GluMet: 2.069 ± 0.265
3.148GluAsn: 3.148 ± 0.355
3.328GluPro: 3.328 ± 0.481
3.013GluGln: 3.013 ± 0.432
1.844GluArg: 1.844 ± 0.288
3.148GluSer: 3.148 ± 0.311
3.238GluThr: 3.238 ± 0.381
4.677GluVal: 4.677 ± 0.6
1.034GluTrp: 1.034 ± 0.2
2.878GluTyr: 2.878 ± 0.381
0.0GluXaa: 0.0 ± 0.0
Phe
2.249PheAla: 2.249 ± 0.284
0.36PheCys: 0.36 ± 0.134
2.519PheAsp: 2.519 ± 0.372
2.024PheGlu: 2.024 ± 0.286
0.81PhePhe: 0.81 ± 0.239
2.474PheGly: 2.474 ± 0.322
0.81PheHis: 0.81 ± 0.205
2.384PheIle: 2.384 ± 0.359
1.799PheLys: 1.799 ± 0.303
2.564PheLeu: 2.564 ± 0.386
1.619PheMet: 1.619 ± 0.256
2.339PheAsn: 2.339 ± 0.375
0.899PhePro: 0.899 ± 0.176
1.439PheGln: 1.439 ± 0.214
2.024PheArg: 2.024 ± 0.287
2.429PheSer: 2.429 ± 0.363
2.519PheThr: 2.519 ± 0.353
1.799PheVal: 1.799 ± 0.313
0.27PheTrp: 0.27 ± 0.114
1.484PheTyr: 1.484 ± 0.231
0.0PheXaa: 0.0 ± 0.0
Gly
5.577GlyAla: 5.577 ± 0.58
0.899GlyCys: 0.899 ± 0.237
3.643GlyAsp: 3.643 ± 0.365
3.868GlyGlu: 3.868 ± 0.345
2.968GlyPhe: 2.968 ± 0.43
3.733GlyGly: 3.733 ± 0.534
1.034GlyHis: 1.034 ± 0.256
4.138GlyIle: 4.138 ± 0.602
6.072GlyLys: 6.072 ± 0.602
4.947GlyLeu: 4.947 ± 0.552
2.114GlyMet: 2.114 ± 0.254
4.812GlyAsn: 4.812 ± 0.458
0.855GlyPro: 0.855 ± 0.173
2.114GlyGln: 2.114 ± 0.294
2.474GlyArg: 2.474 ± 0.451
5.442GlySer: 5.442 ± 0.596
4.273GlyThr: 4.273 ± 0.391
4.318GlyVal: 4.318 ± 0.444
1.124GlyTrp: 1.124 ± 0.257
3.013GlyTyr: 3.013 ± 0.383
0.0GlyXaa: 0.0 ± 0.0
His
1.169HisAla: 1.169 ± 0.208
0.18HisCys: 0.18 ± 0.102
1.034HisAsp: 1.034 ± 0.326
1.214HisGlu: 1.214 ± 0.291
0.495HisPhe: 0.495 ± 0.177
0.855HisGly: 0.855 ± 0.245
0.405HisHis: 0.405 ± 0.195
0.944HisIle: 0.944 ± 0.209
1.304HisLys: 1.304 ± 0.287
2.069HisLeu: 2.069 ± 0.315
0.405HisMet: 0.405 ± 0.127
0.989HisAsn: 0.989 ± 0.169
0.72HisPro: 0.72 ± 0.216
0.675HisGln: 0.675 ± 0.161
0.675HisArg: 0.675 ± 0.151
1.214HisSer: 1.214 ± 0.215
0.765HisThr: 0.765 ± 0.196
0.72HisVal: 0.72 ± 0.175
0.495HisTrp: 0.495 ± 0.143
0.855HisTyr: 0.855 ± 0.16
0.0HisXaa: 0.0 ± 0.0
Ile
4.318IleAla: 4.318 ± 0.469
0.45IleCys: 0.45 ± 0.154
3.553IleAsp: 3.553 ± 0.566
3.508IleGlu: 3.508 ± 0.32
1.484IlePhe: 1.484 ± 0.293
3.238IleGly: 3.238 ± 0.352
1.214IleHis: 1.214 ± 0.262
2.923IleIle: 2.923 ± 0.473
3.868IleLys: 3.868 ± 0.517
4.183IleLeu: 4.183 ± 0.473
1.124IleMet: 1.124 ± 0.175
3.823IleAsn: 3.823 ± 0.526
3.103IlePro: 3.103 ± 0.46
2.609IleGln: 2.609 ± 0.35
3.148IleArg: 3.148 ± 0.439
2.878IleSer: 2.878 ± 0.356
4.677IleThr: 4.677 ± 0.363
3.553IleVal: 3.553 ± 0.423
0.45IleTrp: 0.45 ± 0.18
2.339IleTyr: 2.339 ± 0.415
0.0IleXaa: 0.0 ± 0.0
Lys
6.206LysAla: 6.206 ± 0.629
0.315LysCys: 0.315 ± 0.156
3.238LysAsp: 3.238 ± 0.362
4.722LysGlu: 4.722 ± 0.467
2.609LysPhe: 2.609 ± 0.358
4.093LysGly: 4.093 ± 0.502
1.259LysHis: 1.259 ± 0.286
2.968LysIle: 2.968 ± 0.375
3.373LysLys: 3.373 ± 0.498
6.566LysLeu: 6.566 ± 0.477
2.024LysMet: 2.024 ± 0.335
3.643LysAsn: 3.643 ± 0.418
3.238LysPro: 3.238 ± 0.516
3.058LysGln: 3.058 ± 0.331
2.609LysArg: 2.609 ± 0.353
4.048LysSer: 4.048 ± 0.529
4.362LysThr: 4.362 ± 0.479
4.407LysVal: 4.407 ± 0.491
0.72LysTrp: 0.72 ± 0.204
2.204LysTyr: 2.204 ± 0.444
0.0LysXaa: 0.0 ± 0.0
Leu
7.511LeuAla: 7.511 ± 0.734
0.765LeuCys: 0.765 ± 0.251
4.722LeuAsp: 4.722 ± 0.45
4.362LeuGlu: 4.362 ± 0.406
2.788LeuPhe: 2.788 ± 0.28
6.161LeuGly: 6.161 ± 0.527
1.709LeuHis: 1.709 ± 0.312
4.048LeuIle: 4.048 ± 0.434
4.947LeuLys: 4.947 ± 0.419
5.802LeuLeu: 5.802 ± 0.558
2.564LeuMet: 2.564 ± 0.386
5.082LeuAsn: 5.082 ± 0.505
4.138LeuPro: 4.138 ± 0.456
3.328LeuGln: 3.328 ± 0.481
3.868LeuArg: 3.868 ± 0.492
5.217LeuSer: 5.217 ± 0.416
5.262LeuThr: 5.262 ± 0.588
5.352LeuVal: 5.352 ± 0.479
0.899LeuTrp: 0.899 ± 0.221
2.698LeuTyr: 2.698 ± 0.375
0.0LeuXaa: 0.0 ± 0.0
Met
2.294MetAla: 2.294 ± 0.493
0.135MetCys: 0.135 ± 0.081
1.214MetAsp: 1.214 ± 0.234
2.069MetGlu: 2.069 ± 0.329
0.54MetPhe: 0.54 ± 0.169
1.799MetGly: 1.799 ± 0.248
0.405MetHis: 0.405 ± 0.12
1.709MetIle: 1.709 ± 0.287
2.609MetLys: 2.609 ± 0.375
2.249MetLeu: 2.249 ± 0.42
0.72MetMet: 0.72 ± 0.185
2.339MetAsn: 2.339 ± 0.263
1.214MetPro: 1.214 ± 0.264
1.484MetGln: 1.484 ± 0.282
1.259MetArg: 1.259 ± 0.181
2.429MetSer: 2.429 ± 0.329
1.664MetThr: 1.664 ± 0.329
1.844MetVal: 1.844 ± 0.312
0.225MetTrp: 0.225 ± 0.097
0.81MetTyr: 0.81 ± 0.149
0.0MetXaa: 0.0 ± 0.0
Asn
4.587AsnAla: 4.587 ± 0.595
0.36AsnCys: 0.36 ± 0.162
2.698AsnAsp: 2.698 ± 0.351
3.418AsnGlu: 3.418 ± 0.465
1.799AsnPhe: 1.799 ± 0.233
4.362AsnGly: 4.362 ± 0.44
1.484AsnHis: 1.484 ± 0.213
3.148AsnIle: 3.148 ± 0.39
3.733AsnLys: 3.733 ± 0.372
4.857AsnLeu: 4.857 ± 0.515
1.484AsnMet: 1.484 ± 0.219
3.148AsnAsn: 3.148 ± 0.387
3.373AsnPro: 3.373 ± 0.427
3.553AsnGln: 3.553 ± 0.438
3.598AsnArg: 3.598 ± 0.378
3.238AsnSer: 3.238 ± 0.448
3.328AsnThr: 3.328 ± 0.437
3.148AsnVal: 3.148 ± 0.321
0.72AsnTrp: 0.72 ± 0.204
2.069AsnTyr: 2.069 ± 0.434
0.0AsnXaa: 0.0 ± 0.0
Pro
3.373ProAla: 3.373 ± 0.553
0.27ProCys: 0.27 ± 0.136
2.788ProAsp: 2.788 ± 0.487
4.138ProGlu: 4.138 ± 0.45
1.754ProPhe: 1.754 ± 0.278
2.564ProGly: 2.564 ± 0.293
0.36ProHis: 0.36 ± 0.132
1.889ProIle: 1.889 ± 0.265
1.844ProLys: 1.844 ± 0.263
3.103ProLeu: 3.103 ± 0.329
1.214ProMet: 1.214 ± 0.199
1.889ProAsn: 1.889 ± 0.255
0.944ProPro: 0.944 ± 0.262
1.439ProGln: 1.439 ± 0.24
1.124ProArg: 1.124 ± 0.282
2.653ProSer: 2.653 ± 0.347
3.418ProThr: 3.418 ± 0.444
4.138ProVal: 4.138 ± 0.379
0.63ProTrp: 0.63 ± 0.193
1.529ProTyr: 1.529 ± 0.283
0.0ProXaa: 0.0 ± 0.0
Gln
5.172GlnAla: 5.172 ± 0.874
0.225GlnCys: 0.225 ± 0.114
2.159GlnAsp: 2.159 ± 0.396
3.238GlnGlu: 3.238 ± 0.319
1.619GlnPhe: 1.619 ± 0.218
3.013GlnGly: 3.013 ± 0.484
0.36GlnHis: 0.36 ± 0.142
2.249GlnIle: 2.249 ± 0.258
3.013GlnLys: 3.013 ± 0.588
3.283GlnLeu: 3.283 ± 0.449
1.259GlnMet: 1.259 ± 0.206
1.934GlnAsn: 1.934 ± 0.4
0.989GlnPro: 0.989 ± 0.225
1.754GlnGln: 1.754 ± 0.338
1.979GlnArg: 1.979 ± 0.333
2.294GlnSer: 2.294 ± 0.267
2.429GlnThr: 2.429 ± 0.389
3.193GlnVal: 3.193 ± 0.357
0.495GlnTrp: 0.495 ± 0.177
2.024GlnTyr: 2.024 ± 0.341
0.0GlnXaa: 0.0 ± 0.0
Arg
3.643ArgAla: 3.643 ± 0.524
0.585ArgCys: 0.585 ± 0.195
2.114ArgAsp: 2.114 ± 0.289
2.923ArgGlu: 2.923 ± 0.424
1.619ArgPhe: 1.619 ± 0.243
2.788ArgGly: 2.788 ± 0.359
0.675ArgHis: 0.675 ± 0.191
3.013ArgIle: 3.013 ± 0.523
3.418ArgLys: 3.418 ± 0.363
4.228ArgLeu: 4.228 ± 0.567
1.259ArgMet: 1.259 ± 0.218
3.013ArgAsn: 3.013 ± 0.41
1.574ArgPro: 1.574 ± 0.293
1.979ArgGln: 1.979 ± 0.287
1.574ArgArg: 1.574 ± 0.323
2.878ArgSer: 2.878 ± 0.409
2.429ArgThr: 2.429 ± 0.251
2.384ArgVal: 2.384 ± 0.291
0.63ArgTrp: 0.63 ± 0.202
1.349ArgTyr: 1.349 ± 0.207
0.0ArgXaa: 0.0 ± 0.0
Ser
4.273SerAla: 4.273 ± 0.437
0.63SerCys: 0.63 ± 0.197
3.553SerAsp: 3.553 ± 0.39
3.778SerGlu: 3.778 ± 0.438
2.024SerPhe: 2.024 ± 0.298
4.812SerGly: 4.812 ± 0.449
0.899SerHis: 0.899 ± 0.206
3.418SerIle: 3.418 ± 0.425
4.003SerLys: 4.003 ± 0.485
6.116SerLeu: 6.116 ± 0.546
1.484SerMet: 1.484 ± 0.299
2.878SerAsn: 2.878 ± 0.303
2.519SerPro: 2.519 ± 0.424
2.609SerGln: 2.609 ± 0.425
2.833SerArg: 2.833 ± 0.398
3.868SerSer: 3.868 ± 0.598
4.452SerThr: 4.452 ± 0.531
4.362SerVal: 4.362 ± 0.469
0.81SerTrp: 0.81 ± 0.228
2.204SerTyr: 2.204 ± 0.348
0.0SerXaa: 0.0 ± 0.0
Thr
4.497ThrAla: 4.497 ± 0.587
0.54ThrCys: 0.54 ± 0.176
4.318ThrAsp: 4.318 ± 0.305
3.688ThrGlu: 3.688 ± 0.379
2.923ThrPhe: 2.923 ± 0.256
4.992ThrGly: 4.992 ± 0.562
0.944ThrHis: 0.944 ± 0.26
3.823ThrIle: 3.823 ± 0.408
4.138ThrLys: 4.138 ± 0.376
5.307ThrLeu: 5.307 ± 0.457
1.214ThrMet: 1.214 ± 0.274
3.778ThrAsn: 3.778 ± 0.449
3.598ThrPro: 3.598 ± 0.377
2.384ThrGln: 2.384 ± 0.349
2.788ThrArg: 2.788 ± 0.299
3.553ThrSer: 3.553 ± 0.322
3.778ThrThr: 3.778 ± 0.623
4.587ThrVal: 4.587 ± 0.462
0.63ThrTrp: 0.63 ± 0.196
2.294ThrTyr: 2.294 ± 0.371
0.0ThrXaa: 0.0 ± 0.0
Val
5.757ValAla: 5.757 ± 0.509
0.585ValCys: 0.585 ± 0.184
4.767ValAsp: 4.767 ± 0.449
4.318ValGlu: 4.318 ± 0.417
2.384ValPhe: 2.384 ± 0.401
4.632ValGly: 4.632 ± 0.624
1.664ValHis: 1.664 ± 0.208
3.553ValIle: 3.553 ± 0.658
4.407ValLys: 4.407 ± 0.492
3.958ValLeu: 3.958 ± 0.301
2.339ValMet: 2.339 ± 0.365
4.048ValAsn: 4.048 ± 0.445
2.788ValPro: 2.788 ± 0.451
3.103ValGln: 3.103 ± 0.393
3.283ValArg: 3.283 ± 0.359
3.733ValSer: 3.733 ± 0.529
4.632ValThr: 4.632 ± 0.585
4.677ValVal: 4.677 ± 0.547
0.495ValTrp: 0.495 ± 0.165
2.698ValTyr: 2.698 ± 0.41
0.0ValXaa: 0.0 ± 0.0
Trp
0.899TrpAla: 0.899 ± 0.246
0.135TrpCys: 0.135 ± 0.09
1.259TrpAsp: 1.259 ± 0.323
0.63TrpGlu: 0.63 ± 0.195
0.315TrpPhe: 0.315 ± 0.122
0.81TrpGly: 0.81 ± 0.178
0.27TrpHis: 0.27 ± 0.122
0.45TrpIle: 0.45 ± 0.162
0.899TrpLys: 0.899 ± 0.197
1.124TrpLeu: 1.124 ± 0.238
0.27TrpMet: 0.27 ± 0.113
0.36TrpAsn: 0.36 ± 0.166
0.315TrpPro: 0.315 ± 0.1
0.45TrpGln: 0.45 ± 0.139
0.72TrpArg: 0.72 ± 0.192
0.63TrpSer: 0.63 ± 0.169
0.36TrpThr: 0.36 ± 0.107
1.619TrpVal: 1.619 ± 0.244
0.09TrpTrp: 0.09 ± 0.068
0.54TrpTyr: 0.54 ± 0.164
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.788TyrAla: 2.788 ± 0.376
0.495TyrCys: 0.495 ± 0.162
2.474TyrAsp: 2.474 ± 0.234
2.474TyrGlu: 2.474 ± 0.311
1.574TyrPhe: 1.574 ± 0.299
2.429TyrGly: 2.429 ± 0.319
0.765TyrHis: 0.765 ± 0.19
3.013TyrIle: 3.013 ± 0.501
2.923TyrLys: 2.923 ± 0.573
2.474TyrLeu: 2.474 ± 0.354
0.989TyrMet: 0.989 ± 0.187
2.114TyrAsn: 2.114 ± 0.273
1.934TyrPro: 1.934 ± 0.344
1.529TyrGln: 1.529 ± 0.23
1.709TyrArg: 1.709 ± 0.358
2.519TyrSer: 2.519 ± 0.486
1.484TyrThr: 1.484 ± 0.248
3.148TyrVal: 3.148 ± 0.513
0.45TyrTrp: 0.45 ± 0.118
1.124TyrTyr: 1.124 ± 0.266
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 84 proteins (22236 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski