Botrytis elliptica

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Sclerotiniaceae; Botrytis

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12555 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Z1JU93|A0A4Z1JU93_9HELO Uncharacterized protein OS=Botrytis elliptica OX=278938 GN=BELL_0452g00030 PE=4 SV=1
MM1 pKa = 7.74HH2 pKa = 7.34INILVLAASLCTVVLANYY20 pKa = 9.63PSDD23 pKa = 4.0DD24 pKa = 3.97NKK26 pKa = 11.26DD27 pKa = 3.36AVADD31 pKa = 3.88YY32 pKa = 10.36PFDD35 pKa = 4.81PNQAGVPDD43 pKa = 4.01VVLDD47 pKa = 4.12ASQAAVVSGIVSSLSSLSTALDD69 pKa = 3.49IPSSLASEE77 pKa = 4.94LSNIPTSASTLGYY90 pKa = 9.99SPTGVSQLDD99 pKa = 3.79EE100 pKa = 4.18QLSGTNKK107 pKa = 9.46PDD109 pKa = 2.98WYY111 pKa = 9.96TSLSPDD117 pKa = 2.95AKK119 pKa = 10.62SYY121 pKa = 11.09VDD123 pKa = 3.48NAVSILDD130 pKa = 3.54SVYY133 pKa = 10.83SLEE136 pKa = 5.24SILTTATAWPTTTVGVVGGTTATPLSTWEE165 pKa = 4.28STWASSTKK173 pKa = 10.02FDD175 pKa = 4.22DD176 pKa = 4.28WGSSWASSTGGASWDD191 pKa = 4.05SSWTTSTGADD201 pKa = 3.04SWDD204 pKa = 3.81SSWASSTGVNDD215 pKa = 3.86WEE217 pKa = 4.58STSPSGISSKK227 pKa = 10.77AGEE230 pKa = 4.47SVFGSSTGTKK240 pKa = 10.27DD241 pKa = 2.86STAAQTTGPIQVTTSNATPIMCNLRR266 pKa = 11.84LGFALAVAVGLICAMM281 pKa = 4.47

Molecular weight: 28.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Z1KGX3|A0A4Z1KGX3_9HELO Aminotran_5 domain-containing protein OS=Botrytis elliptica OX=278938 GN=BELL_0007g00130 PE=4 SV=1
MM1 pKa = 7.27SLSHH5 pKa = 5.73ATSWRR10 pKa = 11.84LRR12 pKa = 11.84PIEE15 pKa = 4.29SFNFHH20 pKa = 4.8TTKK23 pKa = 10.66RR24 pKa = 11.84PIPAPLKK31 pKa = 9.79PPPPKK36 pKa = 10.03PPPPKK41 pKa = 9.83PPPPKK46 pKa = 9.83PPPPKK51 pKa = 9.83PPPPKK56 pKa = 9.65PPTMTFSLTLTSLLHH71 pKa = 6.54LLPTLFGTIFILFGLNAMLRR91 pKa = 11.84PLHH94 pKa = 6.85ALTFYY99 pKa = 10.74PSLHH103 pKa = 6.58HH104 pKa = 6.64SLSPSLPQSPSNTILLEE121 pKa = 4.15SLLTIYY127 pKa = 10.32GARR130 pKa = 11.84DD131 pKa = 2.97IFMGPRR137 pKa = 11.84HH138 pKa = 5.81VRR140 pKa = 11.84RR141 pKa = 11.84ILPSQLQNTRR151 pKa = 11.84LDD153 pKa = 3.71RR154 pKa = 11.84GCGEE158 pKa = 4.2WCCVCGWVGVLEE170 pKa = 4.6GWRR173 pKa = 11.84GTGRR177 pKa = 11.84SLGVCARR184 pKa = 11.84TCGCGGFAGVGVLRR198 pKa = 11.84MII200 pKa = 5.03

Molecular weight: 21.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12555

0

12555

5947282

35

6915

473.7

52.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.666 ± 0.019

1.2 ± 0.009

5.586 ± 0.017

6.592 ± 0.025

3.731 ± 0.014

6.729 ± 0.019

2.284 ± 0.011

5.492 ± 0.015

5.472 ± 0.02

8.536 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.241 ± 0.009

4.262 ± 0.013

5.777 ± 0.022

3.881 ± 0.016

5.67 ± 0.019

8.785 ± 0.029

6.145 ± 0.019

5.73 ± 0.015

1.4 ± 0.009

2.821 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski