Salipiger bermudensis (strain DSM 26914 / JCM 13377 / KCTC 12554 / HTCC2601) (Pelagibaca bermudensis)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Salipiger; Salipiger bermudensis

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5420 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0FL30|Q0FL30_SALBH (R)-citramalate synthase OS=Salipiger bermudensis (strain DSM 26914 / JCM 13377 / KCTC 12554 / HTCC2601) OX=314265 GN=R2601_23775 PE=3 SV=1
MM1 pKa = 7.93DD2 pKa = 5.81NDD4 pKa = 3.61VDD6 pKa = 3.8WFATQLDD13 pKa = 3.57ADD15 pKa = 3.55QYY17 pKa = 10.66YY18 pKa = 10.8RR19 pKa = 11.84IDD21 pKa = 4.32LVSASNGDD29 pKa = 3.63VLGLGDD35 pKa = 4.64PYY37 pKa = 11.34FLGLRR42 pKa = 11.84NSQGQIVYY50 pKa = 8.43GTGDD54 pKa = 3.57DD55 pKa = 5.2DD56 pKa = 5.19GGEE59 pKa = 4.76GYY61 pKa = 10.65NSRR64 pKa = 11.84SIFSVAQSGTYY75 pKa = 10.12YY76 pKa = 10.8LAASAFGTSTGAYY89 pKa = 8.09EE90 pKa = 4.75LNLTAYY96 pKa = 10.5DD97 pKa = 3.84FVDD100 pKa = 4.99DD101 pKa = 5.66FGADD105 pKa = 3.62PEE107 pKa = 4.6TSGNFSVGEE116 pKa = 4.25TVSGEE121 pKa = 3.87IQAGDD126 pKa = 3.61VYY128 pKa = 10.83QIQDD132 pKa = 3.99DD133 pKa = 3.82DD134 pKa = 3.72WFAIEE139 pKa = 4.04LTAGEE144 pKa = 4.72SYY146 pKa = 10.84IFDD149 pKa = 3.33VSASGNGTSDD159 pKa = 2.81SGGFPSIEE167 pKa = 3.63GLYY170 pKa = 10.42DD171 pKa = 3.42SEE173 pKa = 4.64GEE175 pKa = 4.88YY176 pKa = 10.59VSEE179 pKa = 4.26WNDD182 pKa = 3.08QVAYY186 pKa = 10.35RR187 pKa = 11.84GNKK190 pKa = 8.99VLFTPDD196 pKa = 2.88EE197 pKa = 4.46SGVYY201 pKa = 9.5HH202 pKa = 6.61VSVTNWDD209 pKa = 3.57YY210 pKa = 11.21FLEE213 pKa = 4.83GEE215 pKa = 4.88GGYY218 pKa = 9.93EE219 pKa = 4.04LQASIYY225 pKa = 10.24QRR227 pKa = 11.84IDD229 pKa = 3.1DD230 pKa = 4.47FAATVDD236 pKa = 3.39TTGILRR242 pKa = 11.84VGEE245 pKa = 4.1AATGEE250 pKa = 4.17IEE252 pKa = 5.52DD253 pKa = 3.85NAFLPRR259 pKa = 11.84ADD261 pKa = 4.22RR262 pKa = 11.84DD263 pKa = 3.37WFAVEE268 pKa = 4.29LEE270 pKa = 4.26EE271 pKa = 5.3GGVYY275 pKa = 9.63QFVMSPTDD283 pKa = 3.35ATGSLSYY290 pKa = 10.52SAVIRR295 pKa = 11.84GIYY298 pKa = 9.96DD299 pKa = 3.23SEE301 pKa = 6.04GDD303 pKa = 4.63LIPVMVRR310 pKa = 11.84NSYY313 pKa = 10.36YY314 pKa = 10.73LSNSLFFAPEE324 pKa = 3.55ANGTYY329 pKa = 9.6FVSASAEE336 pKa = 3.98WVDD339 pKa = 3.53VYY341 pKa = 11.24GAYY344 pKa = 9.91EE345 pKa = 4.01ISAEE349 pKa = 4.19RR350 pKa = 11.84IYY352 pKa = 11.34SFDD355 pKa = 3.44GYY357 pKa = 11.48VPDD360 pKa = 4.65AVASIQIHH368 pKa = 6.48AYY370 pKa = 8.8SPNYY374 pKa = 9.81VDD376 pKa = 5.22DD377 pKa = 4.8ASSTLVFGSDD387 pKa = 3.1RR388 pKa = 11.84DD389 pKa = 3.7NTIRR393 pKa = 11.84GISGHH398 pKa = 6.66DD399 pKa = 3.31VLLGAEE405 pKa = 4.68GDD407 pKa = 4.06DD408 pKa = 5.14LLIGDD413 pKa = 4.85DD414 pKa = 3.83TADD417 pKa = 4.0GQWLPGIEE425 pKa = 3.85GAVFRR430 pKa = 11.84LFEE433 pKa = 4.13FLLDD437 pKa = 3.78RR438 pKa = 11.84EE439 pKa = 4.15PSEE442 pKa = 5.98RR443 pKa = 11.84EE444 pKa = 3.64LQMGLEE450 pKa = 3.9NAYY453 pKa = 10.61YY454 pKa = 10.4SFYY457 pKa = 11.03GLSDD461 pKa = 3.33AAAFIIGSPEE471 pKa = 3.49FDD473 pKa = 3.58QRR475 pKa = 11.84WGDD478 pKa = 3.37LHH480 pKa = 6.87GADD483 pKa = 5.0LISFFYY489 pKa = 10.64EE490 pKa = 3.66RR491 pKa = 11.84LRR493 pKa = 11.84GNVVDD498 pKa = 4.69DD499 pKa = 3.99DD500 pKa = 4.49PYY502 pKa = 11.37RR503 pKa = 11.84EE504 pKa = 3.73IWLDD508 pKa = 3.43QLEE511 pKa = 4.38RR512 pKa = 11.84AYY514 pKa = 10.99SWGLLPRR521 pKa = 11.84YY522 pKa = 10.0AEE524 pKa = 4.31GEE526 pKa = 3.96IASAFVLSEE535 pKa = 4.45EE536 pKa = 4.37YY537 pKa = 10.29QSQSSGYY544 pKa = 9.44DD545 pKa = 2.97LTGRR549 pKa = 11.84LVEE552 pKa = 6.29FEE554 pKa = 4.39NQWGDD559 pKa = 3.69EE560 pKa = 4.33VFSSYY565 pKa = 9.75QAVLGRR571 pKa = 11.84RR572 pKa = 11.84PDD574 pKa = 3.23ARR576 pKa = 11.84GFEE579 pKa = 3.99NWLDD583 pKa = 4.1RR584 pKa = 11.84IEE586 pKa = 4.73NGLDD590 pKa = 3.32LSGLTRR596 pKa = 11.84MLLTSQEE603 pKa = 4.46FEE605 pKa = 4.02QSASGLSDD613 pKa = 3.27RR614 pKa = 11.84DD615 pKa = 3.55FVEE618 pKa = 5.5LVLTNLDD625 pKa = 3.67DD626 pKa = 3.8TTSEE630 pKa = 4.38VEE632 pKa = 3.94VDD634 pKa = 4.58FAAAQLDD641 pKa = 3.82AGLSRR646 pKa = 11.84DD647 pKa = 3.48EE648 pKa = 4.37FVAVQVLLNDD658 pKa = 4.01DD659 pKa = 3.83QGFDD663 pKa = 2.49AWMRR667 pKa = 11.84ARR669 pKa = 11.84GEE671 pKa = 4.13DD672 pKa = 3.66DD673 pKa = 3.6VLAGGAGNDD682 pKa = 4.16LLFGGPGSDD691 pKa = 2.47RR692 pKa = 11.84FVFAPDD698 pKa = 3.37EE699 pKa = 4.71GNDD702 pKa = 3.57TVADD706 pKa = 4.09LQSFDD711 pKa = 5.14IIDD714 pKa = 3.72LMAFGFADD722 pKa = 4.21LDD724 pKa = 3.76AALEE728 pKa = 4.28AFHH731 pKa = 6.82QDD733 pKa = 2.49GDD735 pKa = 4.14HH736 pKa = 6.99LVFEE740 pKa = 5.12AEE742 pKa = 3.82NTRR745 pKa = 11.84IRR747 pKa = 11.84FQSEE751 pKa = 3.44QLAALTDD758 pKa = 3.81DD759 pKa = 4.01MLQVV763 pKa = 3.39

Molecular weight: 83.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0FM68|Q0FM68_SALBH Pseudouridine synthase OS=Salipiger bermudensis (strain DSM 26914 / JCM 13377 / KCTC 12554 / HTCC2601) OX=314265 GN=R2601_17007 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84QILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5420

0

5420

1601589

16

10077

295.5

31.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.619 ± 0.045

0.901 ± 0.014

5.932 ± 0.048

6.361 ± 0.037

3.566 ± 0.019

8.906 ± 0.042

2.036 ± 0.022

4.873 ± 0.024

2.748 ± 0.027

10.27 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.779 ± 0.023

2.326 ± 0.027

5.271 ± 0.041

3.082 ± 0.018

6.947 ± 0.051

5.267 ± 0.037

5.393 ± 0.036

7.206 ± 0.031

1.383 ± 0.014

2.137 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski