Amino acid dipepetide frequency for Gramella flava JLT2011

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.964AlaAla: 4.964 ± 0.082
0.529AlaCys: 0.529 ± 0.025
3.647AlaAsp: 3.647 ± 0.102
5.313AlaGlu: 5.313 ± 0.074
3.226AlaPhe: 3.226 ± 0.065
4.866AlaGly: 4.866 ± 0.085
1.12AlaHis: 1.12 ± 0.031
5.132AlaIle: 5.132 ± 0.078
4.11AlaLys: 4.11 ± 0.076
5.916AlaLeu: 5.916 ± 0.082
1.693AlaMet: 1.693 ± 0.038
3.191AlaAsn: 3.191 ± 0.061
1.985AlaPro: 1.985 ± 0.046
2.466AlaGln: 2.466 ± 0.041
2.517AlaArg: 2.517 ± 0.05
4.385AlaSer: 4.385 ± 0.066
3.449AlaThr: 3.449 ± 0.083
4.303AlaVal: 4.303 ± 0.08
0.675AlaTrp: 0.675 ± 0.027
2.557AlaTyr: 2.557 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
0.413CysAla: 0.413 ± 0.019
0.103CysCys: 0.103 ± 0.009
0.515CysAsp: 0.515 ± 0.102
0.507CysGlu: 0.507 ± 0.026
0.356CysPhe: 0.356 ± 0.018
0.621CysGly: 0.621 ± 0.031
0.159CysHis: 0.159 ± 0.013
0.474CysIle: 0.474 ± 0.021
0.373CysLys: 0.373 ± 0.019
0.593CysLeu: 0.593 ± 0.025
0.129CysMet: 0.129 ± 0.01
0.312CysAsn: 0.312 ± 0.017
0.313CysPro: 0.313 ± 0.026
0.231CysGln: 0.231 ± 0.017
0.222CysArg: 0.222 ± 0.014
0.558CysSer: 0.558 ± 0.028
0.347CysThr: 0.347 ± 0.021
0.382CysVal: 0.382 ± 0.018
0.057CysTrp: 0.057 ± 0.007
0.265CysTyr: 0.265 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
3.735AspAla: 3.735 ± 0.072
0.429AspCys: 0.429 ± 0.047
2.981AspAsp: 2.981 ± 0.063
4.072AspGlu: 4.072 ± 0.067
3.847AspPhe: 3.847 ± 0.057
4.093AspGly: 4.093 ± 0.285
1.212AspHis: 1.212 ± 0.033
4.004AspIle: 4.004 ± 0.067
3.389AspLys: 3.389 ± 0.064
5.573AspLeu: 5.573 ± 0.079
1.194AspMet: 1.194 ± 0.029
2.645AspAsn: 2.645 ± 0.078
2.279AspPro: 2.279 ± 0.129
2.154AspGln: 2.154 ± 0.048
2.394AspArg: 2.394 ± 0.043
3.539AspSer: 3.539 ± 0.097
2.597AspThr: 2.597 ± 0.055
3.31AspVal: 3.31 ± 0.06
0.801AspTrp: 0.801 ± 0.033
2.9AspTyr: 2.9 ± 0.054
0.0AspXaa: 0.0 ± 0.0
Glu
4.857GluAla: 4.857 ± 0.074
0.337GluCys: 0.337 ± 0.023
4.321GluAsp: 4.321 ± 0.071
6.358GluGlu: 6.358 ± 0.09
3.257GluPhe: 3.257 ± 0.055
4.233GluGly: 4.233 ± 0.076
1.296GluHis: 1.296 ± 0.034
6.1GluIle: 6.1 ± 0.075
6.631GluLys: 6.631 ± 0.098
6.73GluLeu: 6.73 ± 0.084
1.949GluMet: 1.949 ± 0.043
5.443GluAsn: 5.443 ± 0.07
1.91GluPro: 1.91 ± 0.048
2.624GluGln: 2.624 ± 0.053
2.82GluArg: 2.82 ± 0.059
3.364GluSer: 3.364 ± 0.051
3.848GluThr: 3.848 ± 0.055
4.845GluVal: 4.845 ± 0.077
0.711GluTrp: 0.711 ± 0.025
2.655GluTyr: 2.655 ± 0.048
0.0GluXaa: 0.0 ± 0.0
Phe
2.986PheAla: 2.986 ± 0.044
0.451PheCys: 0.451 ± 0.019
3.14PheAsp: 3.14 ± 0.061
3.622PheGlu: 3.622 ± 0.057
2.67PhePhe: 2.67 ± 0.058
3.74PheGly: 3.74 ± 0.069
0.965PheHis: 0.965 ± 0.027
3.472PheIle: 3.472 ± 0.059
3.085PheLys: 3.085 ± 0.054
5.094PheLeu: 5.094 ± 0.088
1.16PheMet: 1.16 ± 0.035
2.719PheAsn: 2.719 ± 0.054
1.882PhePro: 1.882 ± 0.05
2.228PheGln: 2.228 ± 0.046
2.272PheArg: 2.272 ± 0.047
4.224PheSer: 4.224 ± 0.074
2.851PheThr: 2.851 ± 0.061
2.731PheVal: 2.731 ± 0.05
0.648PheTrp: 0.648 ± 0.026
2.166PheTyr: 2.166 ± 0.045
0.0PheXaa: 0.0 ± 0.0
Gly
4.157GlyAla: 4.157 ± 0.072
0.548GlyCys: 0.548 ± 0.028
3.751GlyAsp: 3.751 ± 0.086
4.355GlyGlu: 4.355 ± 0.065
3.565GlyPhe: 3.565 ± 0.063
4.699GlyGly: 4.699 ± 0.08
1.213GlyHis: 1.213 ± 0.034
5.521GlyIle: 5.521 ± 0.092
5.174GlyLys: 5.174 ± 0.073
5.822GlyLeu: 5.822 ± 0.092
1.853GlyMet: 1.853 ± 0.044
3.802GlyAsn: 3.802 ± 0.082
1.5GlyPro: 1.5 ± 0.041
2.133GlyGln: 2.133 ± 0.066
2.387GlyArg: 2.387 ± 0.047
4.271GlySer: 4.271 ± 0.078
3.937GlyThr: 3.937 ± 0.122
4.216GlyVal: 4.216 ± 0.07
0.859GlyTrp: 0.859 ± 0.029
2.861GlyTyr: 2.861 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
0.979HisAla: 0.979 ± 0.03
0.167HisCys: 0.167 ± 0.011
0.869HisAsp: 0.869 ± 0.028
1.136HisGlu: 1.136 ± 0.034
1.238HisPhe: 1.238 ± 0.031
1.145HisGly: 1.145 ± 0.035
0.513HisHis: 0.513 ± 0.024
1.323HisIle: 1.323 ± 0.037
1.002HisLys: 1.002 ± 0.03
1.796HisLeu: 1.796 ± 0.042
0.368HisMet: 0.368 ± 0.019
0.863HisAsn: 0.863 ± 0.03
0.961HisPro: 0.961 ± 0.028
0.853HisGln: 0.853 ± 0.028
0.764HisArg: 0.764 ± 0.025
1.201HisSer: 1.201 ± 0.034
0.937HisThr: 0.937 ± 0.029
0.902HisVal: 0.902 ± 0.025
0.249HisTrp: 0.249 ± 0.015
0.832HisTyr: 0.832 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
5.26IleAla: 5.26 ± 0.081
0.599IleCys: 0.599 ± 0.033
4.434IleAsp: 4.434 ± 0.067
4.957IleGlu: 4.957 ± 0.073
3.83IlePhe: 3.83 ± 0.064
4.87IleGly: 4.87 ± 0.079
1.32IleHis: 1.32 ± 0.037
4.952IleIle: 4.952 ± 0.091
4.403IleLys: 4.403 ± 0.071
7.029IleLeu: 7.029 ± 0.102
1.346IleMet: 1.346 ± 0.033
3.824IleAsn: 3.824 ± 0.064
3.312IlePro: 3.312 ± 0.053
2.803IleGln: 2.803 ± 0.046
3.033IleArg: 3.033 ± 0.058
5.657IleSer: 5.657 ± 0.072
3.957IleThr: 3.957 ± 0.089
4.115IleVal: 4.115 ± 0.061
0.714IleTrp: 0.714 ± 0.024
2.689IleTyr: 2.689 ± 0.051
0.0IleXaa: 0.0 ± 0.0
Lys
4.609LysAla: 4.609 ± 0.075
0.284LysCys: 0.284 ± 0.016
4.184LysAsp: 4.184 ± 0.07
5.69LysGlu: 5.69 ± 0.091
2.875LysPhe: 2.875 ± 0.054
3.981LysGly: 3.981 ± 0.071
1.18LysHis: 1.18 ± 0.035
5.645LysIle: 5.645 ± 0.083
6.153LysLys: 6.153 ± 0.105
6.596LysLeu: 6.596 ± 0.099
2.062LysMet: 2.062 ± 0.044
4.535LysAsn: 4.535 ± 0.07
2.503LysPro: 2.503 ± 0.044
2.295LysGln: 2.295 ± 0.052
2.825LysArg: 2.825 ± 0.057
3.95LysSer: 3.95 ± 0.062
3.859LysThr: 3.859 ± 0.068
4.206LysVal: 4.206 ± 0.065
0.76LysTrp: 0.76 ± 0.027
2.615LysTyr: 2.615 ± 0.056
0.0LysXaa: 0.0 ± 0.0
Leu
6.166LeuAla: 6.166 ± 0.078
0.622LeuCys: 0.622 ± 0.029
5.156LeuAsp: 5.156 ± 0.085
6.73LeuGlu: 6.73 ± 0.074
4.679LeuPhe: 4.679 ± 0.077
5.872LeuGly: 5.872 ± 0.074
1.633LeuHis: 1.633 ± 0.041
6.461LeuIle: 6.461 ± 0.084
7.61LeuLys: 7.61 ± 0.095
8.762LeuLeu: 8.762 ± 0.116
2.157LeuMet: 2.157 ± 0.045
5.201LeuAsn: 5.201 ± 0.081
3.718LeuPro: 3.718 ± 0.071
4.066LeuGln: 4.066 ± 0.073
3.621LeuArg: 3.621 ± 0.061
6.524LeuSer: 6.524 ± 0.078
4.556LeuThr: 4.556 ± 0.073
5.466LeuVal: 5.466 ± 0.076
0.894LeuTrp: 0.894 ± 0.03
3.126LeuTyr: 3.126 ± 0.051
0.0LeuXaa: 0.0 ± 0.0
Met
1.853MetAla: 1.853 ± 0.041
0.108MetCys: 0.108 ± 0.011
1.29MetAsp: 1.29 ± 0.035
1.774MetGlu: 1.774 ± 0.044
0.813MetPhe: 0.813 ± 0.027
1.564MetGly: 1.564 ± 0.047
0.411MetHis: 0.411 ± 0.021
1.665MetIle: 1.665 ± 0.041
2.236MetLys: 2.236 ± 0.048
2.177MetLeu: 2.177 ± 0.044
0.67MetMet: 0.67 ± 0.03
1.382MetAsn: 1.382 ± 0.032
0.942MetPro: 0.942 ± 0.031
0.978MetGln: 0.978 ± 0.035
0.958MetArg: 0.958 ± 0.029
1.365MetSer: 1.365 ± 0.037
1.096MetThr: 1.096 ± 0.029
1.408MetVal: 1.408 ± 0.043
0.184MetTrp: 0.184 ± 0.013
0.703MetTyr: 0.703 ± 0.023
0.0MetXaa: 0.0 ± 0.0
Asn
3.616AsnAla: 3.616 ± 0.077
0.443AsnCys: 0.443 ± 0.04
3.156AsnAsp: 3.156 ± 0.094
3.493AsnGlu: 3.493 ± 0.066
3.229AsnPhe: 3.229 ± 0.062
3.944AsnGly: 3.944 ± 0.074
0.907AsnHis: 0.907 ± 0.031
3.961AsnIle: 3.961 ± 0.058
3.35AsnLys: 3.35 ± 0.063
5.328AsnLeu: 5.328 ± 0.067
1.347AsnMet: 1.347 ± 0.034
2.871AsnAsn: 2.871 ± 0.09
2.6AsnPro: 2.6 ± 0.064
2.101AsnGln: 2.101 ± 0.041
2.351AsnArg: 2.351 ± 0.048
3.825AsnSer: 3.825 ± 0.061
2.981AsnThr: 2.981 ± 0.069
2.939AsnVal: 2.939 ± 0.056
0.829AsnTrp: 0.829 ± 0.028
2.674AsnTyr: 2.674 ± 0.051
0.0AsnXaa: 0.0 ± 0.0
Pro
2.355ProAla: 2.355 ± 0.051
0.195ProCys: 0.195 ± 0.014
2.373ProAsp: 2.373 ± 0.069
3.809ProGlu: 3.809 ± 0.055
1.867ProPhe: 1.867 ± 0.048
2.496ProGly: 2.496 ± 0.055
0.635ProHis: 0.635 ± 0.023
2.321ProIle: 2.321 ± 0.038
2.41ProLys: 2.41 ± 0.048
2.969ProLeu: 2.969 ± 0.083
0.813ProMet: 0.813 ± 0.029
1.976ProAsn: 1.976 ± 0.082
0.86ProPro: 0.86 ± 0.031
1.351ProGln: 1.351 ± 0.035
1.028ProArg: 1.028 ± 0.027
2.118ProSer: 2.118 ± 0.049
1.644ProThr: 1.644 ± 0.044
2.933ProVal: 2.933 ± 0.053
0.379ProTrp: 0.379 ± 0.02
1.437ProTyr: 1.437 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
2.473GlnAla: 2.473 ± 0.047
0.135GlnCys: 0.135 ± 0.011
2.068GlnAsp: 2.068 ± 0.05
3.075GlnGlu: 3.075 ± 0.073
1.854GlnPhe: 1.854 ± 0.045
1.91GlnGly: 1.91 ± 0.036
0.68GlnHis: 0.68 ± 0.024
2.673GlnIle: 2.673 ± 0.045
3.153GlnLys: 3.153 ± 0.062
3.863GlnLeu: 3.863 ± 0.067
0.854GlnMet: 0.854 ± 0.03
2.335GlnAsn: 2.335 ± 0.045
1.349GlnPro: 1.349 ± 0.037
1.945GlnGln: 1.945 ± 0.048
1.462GlnArg: 1.462 ± 0.037
2.028GlnSer: 2.028 ± 0.044
1.911GlnThr: 1.911 ± 0.05
2.321GlnVal: 2.321 ± 0.049
0.378GlnTrp: 0.378 ± 0.021
1.413GlnTyr: 1.413 ± 0.032
0.0GlnXaa: 0.0 ± 0.0
Arg
2.282ArgAla: 2.282 ± 0.047
0.189ArgCys: 0.189 ± 0.013
2.172ArgAsp: 2.172 ± 0.042
3.037ArgGlu: 3.037 ± 0.058
2.233ArgPhe: 2.233 ± 0.046
2.163ArgGly: 2.163 ± 0.045
0.711ArgHis: 0.711 ± 0.028
3.115ArgIle: 3.115 ± 0.057
3.47ArgLys: 3.47 ± 0.067
3.686ArgLeu: 3.686 ± 0.06
0.993ArgMet: 0.993 ± 0.029
2.61ArgAsn: 2.61 ± 0.052
1.275ArgPro: 1.275 ± 0.034
1.451ArgGln: 1.451 ± 0.038
1.59ArgArg: 1.59 ± 0.042
2.384ArgSer: 2.384 ± 0.052
1.893ArgThr: 1.893 ± 0.043
2.178ArgVal: 2.178 ± 0.047
0.425ArgTrp: 0.425 ± 0.019
1.668ArgTyr: 1.668 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
4.171SerAla: 4.171 ± 0.061
0.609SerCys: 0.609 ± 0.026
3.448SerAsp: 3.448 ± 0.084
5.254SerGlu: 5.254 ± 0.065
3.736SerPhe: 3.736 ± 0.062
5.089SerGly: 5.089 ± 0.103
1.141SerHis: 1.141 ± 0.034
4.68SerIle: 4.68 ± 0.063
4.144SerLys: 4.144 ± 0.066
6.183SerLeu: 6.183 ± 0.081
1.441SerMet: 1.441 ± 0.035
3.27SerAsn: 3.27 ± 0.067
2.155SerPro: 2.155 ± 0.049
2.18SerGln: 2.18 ± 0.038
2.705SerArg: 2.705 ± 0.052
4.363SerSer: 4.363 ± 0.093
3.073SerThr: 3.073 ± 0.057
4.058SerVal: 4.058 ± 0.067
0.778SerTrp: 0.778 ± 0.025
2.717SerTyr: 2.717 ± 0.058
0.0SerXaa: 0.0 ± 0.0
Thr
3.9ThrAla: 3.9 ± 0.084
0.343ThrCys: 0.343 ± 0.028
3.124ThrAsp: 3.124 ± 0.111
3.635ThrGlu: 3.635 ± 0.073
2.512ThrPhe: 2.512 ± 0.051
4.373ThrGly: 4.373 ± 0.072
0.881ThrHis: 0.881 ± 0.031
3.868ThrIle: 3.868 ± 0.073
2.898ThrLys: 2.898 ± 0.052
4.323ThrLeu: 4.323 ± 0.063
0.939ThrMet: 0.939 ± 0.029
2.734ThrAsn: 2.734 ± 0.077
2.216ThrPro: 2.216 ± 0.044
1.577ThrGln: 1.577 ± 0.041
1.949ThrArg: 1.949 ± 0.044
3.617ThrSer: 3.617 ± 0.066
2.929ThrThr: 2.929 ± 0.092
3.413ThrVal: 3.413 ± 0.119
0.536ThrTrp: 0.536 ± 0.025
2.236ThrTyr: 2.236 ± 0.055
0.0ThrXaa: 0.0 ± 0.0
Val
4.215ValAla: 4.215 ± 0.076
0.473ValCys: 0.473 ± 0.02
3.35ValAsp: 3.35 ± 0.059
4.081ValGlu: 4.081 ± 0.068
3.194ValPhe: 3.194 ± 0.056
3.639ValGly: 3.639 ± 0.065
1.045ValHis: 1.045 ± 0.033
4.592ValIle: 4.592 ± 0.061
3.922ValLys: 3.922 ± 0.062
5.645ValLeu: 5.645 ± 0.077
1.504ValMet: 1.504 ± 0.039
3.28ValAsn: 3.28 ± 0.065
2.318ValPro: 2.318 ± 0.046
2.132ValGln: 2.132 ± 0.039
2.223ValArg: 2.223 ± 0.046
4.404ValSer: 4.404 ± 0.056
3.459ValThr: 3.459 ± 0.12
3.97ValVal: 3.97 ± 0.069
0.638ValTrp: 0.638 ± 0.026
2.324ValTyr: 2.324 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
0.642TrpAla: 0.642 ± 0.025
0.074TrpCys: 0.074 ± 0.007
0.673TrpAsp: 0.673 ± 0.026
0.729TrpGlu: 0.729 ± 0.026
0.574TrpPhe: 0.574 ± 0.023
0.651TrpGly: 0.651 ± 0.027
0.256TrpHis: 0.256 ± 0.015
0.774TrpIle: 0.774 ± 0.026
0.916TrpLys: 0.916 ± 0.032
1.002TrpLeu: 1.002 ± 0.032
0.35TrpMet: 0.35 ± 0.016
0.775TrpAsn: 0.775 ± 0.026
0.34TrpPro: 0.34 ± 0.019
0.461TrpGln: 0.461 ± 0.02
0.448TrpArg: 0.448 ± 0.02
0.652TrpSer: 0.652 ± 0.025
0.574TrpThr: 0.574 ± 0.027
0.627TrpVal: 0.627 ± 0.026
0.173TrpTrp: 0.173 ± 0.013
0.493TrpTyr: 0.493 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.412TyrAla: 2.412 ± 0.042
0.29TyrCys: 0.29 ± 0.015
2.494TyrAsp: 2.494 ± 0.051
2.665TyrGlu: 2.665 ± 0.05
2.552TyrPhe: 2.552 ± 0.056
2.676TyrGly: 2.676 ± 0.049
0.822TyrHis: 0.822 ± 0.03
2.369TyrIle: 2.369 ± 0.047
2.378TyrLys: 2.378 ± 0.045
3.93TyrLeu: 3.93 ± 0.062
0.754TyrMet: 0.754 ± 0.026
2.177TyrAsn: 2.177 ± 0.043
1.551TyrPro: 1.551 ± 0.035
1.852TyrGln: 1.852 ± 0.04
1.953TyrArg: 1.953 ± 0.04
2.764TyrSer: 2.764 ± 0.049
2.105TyrThr: 2.105 ± 0.053
2.091TyrVal: 2.091 ± 0.036
0.496TyrTrp: 0.496 ± 0.022
1.817TyrTyr: 1.817 ± 0.043
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3628 proteins (1223118 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski