Amino acid dipepetide frequency for Bacillus caeni

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.642AlaAla: 5.642 ± 0.087
0.554AlaCys: 0.554 ± 0.021
3.392AlaAsp: 3.392 ± 0.051
4.855AlaGlu: 4.855 ± 0.07
3.341AlaPhe: 3.341 ± 0.052
5.576AlaGly: 5.576 ± 0.076
1.171AlaHis: 1.171 ± 0.032
5.514AlaIle: 5.514 ± 0.078
4.52AlaLys: 4.52 ± 0.066
6.852AlaLeu: 6.852 ± 0.083
1.983AlaMet: 1.983 ± 0.04
2.692AlaAsn: 2.692 ± 0.047
2.025AlaPro: 2.025 ± 0.04
1.864AlaGln: 1.864 ± 0.04
2.745AlaArg: 2.745 ± 0.055
4.201AlaSer: 4.201 ± 0.065
3.268AlaThr: 3.268 ± 0.052
5.648AlaVal: 5.648 ± 0.063
0.599AlaTrp: 0.599 ± 0.024
2.305AlaTyr: 2.305 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
0.387CysAla: 0.387 ± 0.018
0.093CysCys: 0.093 ± 0.009
0.395CysAsp: 0.395 ± 0.018
0.434CysGlu: 0.434 ± 0.017
0.322CysPhe: 0.322 ± 0.014
0.598CysGly: 0.598 ± 0.021
0.175CysHis: 0.175 ± 0.013
0.43CysIle: 0.43 ± 0.017
0.38CysLys: 0.38 ± 0.017
0.645CysLeu: 0.645 ± 0.027
0.17CysMet: 0.17 ± 0.011
0.287CysAsn: 0.287 ± 0.013
0.349CysPro: 0.349 ± 0.019
0.194CysGln: 0.194 ± 0.012
0.292CysArg: 0.292 ± 0.016
0.476CysSer: 0.476 ± 0.019
0.392CysThr: 0.392 ± 0.016
0.374CysVal: 0.374 ± 0.018
0.075CysTrp: 0.075 ± 0.006
0.251CysTyr: 0.251 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
3.205AspAla: 3.205 ± 0.047
0.372AspCys: 0.372 ± 0.017
2.454AspAsp: 2.454 ± 0.056
4.415AspGlu: 4.415 ± 0.064
2.539AspPhe: 2.539 ± 0.048
3.529AspGly: 3.529 ± 0.063
1.169AspHis: 1.169 ± 0.029
3.917AspIle: 3.917 ± 0.055
3.412AspLys: 3.412 ± 0.056
5.193AspLeu: 5.193 ± 0.074
1.324AspMet: 1.324 ± 0.032
1.984AspAsn: 1.984 ± 0.04
2.161AspPro: 2.161 ± 0.037
1.999AspGln: 1.999 ± 0.042
2.494AspArg: 2.494 ± 0.044
2.819AspSer: 2.819 ± 0.053
2.387AspThr: 2.387 ± 0.042
3.7AspVal: 3.7 ± 0.051
0.713AspTrp: 0.713 ± 0.024
2.26AspTyr: 2.26 ± 0.043
0.0AspXaa: 0.0 ± 0.0
Glu
5.586GluAla: 5.586 ± 0.068
0.389GluCys: 0.389 ± 0.016
4.15GluAsp: 4.15 ± 0.066
8.081GluGlu: 8.081 ± 0.091
2.571GluPhe: 2.571 ± 0.048
4.881GluGly: 4.881 ± 0.066
1.474GluHis: 1.474 ± 0.032
5.655GluIle: 5.655 ± 0.072
7.296GluLys: 7.296 ± 0.09
7.228GluLeu: 7.228 ± 0.083
2.3GluMet: 2.3 ± 0.045
4.153GluAsn: 4.153 ± 0.045
2.08GluPro: 2.08 ± 0.047
3.328GluGln: 3.328 ± 0.058
3.689GluArg: 3.689 ± 0.06
3.759GluSer: 3.759 ± 0.056
4.156GluThr: 4.156 ± 0.062
5.359GluVal: 5.359 ± 0.071
0.907GluTrp: 0.907 ± 0.023
2.29GluTyr: 2.29 ± 0.037
0.0GluXaa: 0.0 ± 0.0
Phe
2.971PheAla: 2.971 ± 0.053
0.319PheCys: 0.319 ± 0.016
2.392PheAsp: 2.392 ± 0.045
2.943PheGlu: 2.943 ± 0.045
2.348PhePhe: 2.348 ± 0.055
3.401PheGly: 3.401 ± 0.059
1.012PheHis: 1.012 ± 0.027
3.718PheIle: 3.718 ± 0.065
2.542PheLys: 2.542 ± 0.046
4.706PheLeu: 4.706 ± 0.068
1.136PheMet: 1.136 ± 0.033
1.98PheAsn: 1.98 ± 0.041
1.778PhePro: 1.778 ± 0.034
1.507PheGln: 1.507 ± 0.035
1.608PheArg: 1.608 ± 0.032
3.295PheSer: 3.295 ± 0.045
2.468PheThr: 2.468 ± 0.045
3.09PheVal: 3.09 ± 0.051
0.503PheTrp: 0.503 ± 0.02
1.776PheTyr: 1.776 ± 0.043
0.0PheXaa: 0.0 ± 0.0
Gly
4.819GlyAla: 4.819 ± 0.086
0.595GlyCys: 0.595 ± 0.02
3.353GlyAsp: 3.353 ± 0.058
4.895GlyGlu: 4.895 ± 0.062
3.614GlyPhe: 3.614 ± 0.061
5.104GlyGly: 5.104 ± 0.081
1.419GlyHis: 1.419 ± 0.038
6.137GlyIle: 6.137 ± 0.071
5.492GlyLys: 5.492 ± 0.063
6.757GlyLeu: 6.757 ± 0.08
2.14GlyMet: 2.14 ± 0.04
3.092GlyAsn: 3.092 ± 0.055
1.928GlyPro: 1.928 ± 0.039
2.119GlyGln: 2.119 ± 0.045
2.667GlyArg: 2.667 ± 0.045
4.137GlySer: 4.137 ± 0.059
4.035GlyThr: 4.035 ± 0.057
5.068GlyVal: 5.068 ± 0.072
0.858GlyTrp: 0.858 ± 0.024
2.903GlyTyr: 2.903 ± 0.048
0.0GlyXaa: 0.0 ± 0.0
His
1.209HisAla: 1.209 ± 0.03
0.186HisCys: 0.186 ± 0.013
1.005HisAsp: 1.005 ± 0.03
1.305HisGlu: 1.305 ± 0.034
1.074HisPhe: 1.074 ± 0.028
1.383HisGly: 1.383 ± 0.033
0.628HisHis: 0.628 ± 0.027
1.309HisIle: 1.309 ± 0.03
1.038HisLys: 1.038 ± 0.029
2.037HisLeu: 2.037 ± 0.04
0.464HisMet: 0.464 ± 0.018
0.843HisAsn: 0.843 ± 0.024
1.13HisPro: 1.13 ± 0.028
0.777HisGln: 0.777 ± 0.023
0.874HisArg: 0.874 ± 0.027
1.242HisSer: 1.242 ± 0.032
0.997HisThr: 0.997 ± 0.028
1.336HisVal: 1.336 ± 0.031
0.225HisTrp: 0.225 ± 0.014
0.871HisTyr: 0.871 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
5.705IleAla: 5.705 ± 0.072
0.565IleCys: 0.565 ± 0.02
4.349IleAsp: 4.349 ± 0.06
5.789IleGlu: 5.789 ± 0.073
3.053IlePhe: 3.053 ± 0.056
6.012IleGly: 6.012 ± 0.078
1.537IleHis: 1.537 ± 0.041
5.218IleIle: 5.218 ± 0.076
4.815IleLys: 4.815 ± 0.072
6.894IleLeu: 6.894 ± 0.087
1.656IleMet: 1.656 ± 0.034
3.3IleAsn: 3.3 ± 0.05
3.336IlePro: 3.336 ± 0.05
2.563IleGln: 2.563 ± 0.048
2.896IleArg: 2.896 ± 0.047
4.849IleSer: 4.849 ± 0.064
4.114IleThr: 4.114 ± 0.055
5.419IleVal: 5.419 ± 0.064
0.65IleTrp: 0.65 ± 0.023
2.328IleTyr: 2.328 ± 0.044
0.0IleXaa: 0.0 ± 0.0
Lys
4.969LysAla: 4.969 ± 0.073
0.318LysCys: 0.318 ± 0.016
4.012LysAsp: 4.012 ± 0.061
7.694LysGlu: 7.694 ± 0.086
1.896LysPhe: 1.896 ± 0.038
4.748LysGly: 4.748 ± 0.06
1.383LysHis: 1.383 ± 0.031
4.577LysIle: 4.577 ± 0.06
6.411LysLys: 6.411 ± 0.086
5.888LysLeu: 5.888 ± 0.069
2.156LysMet: 2.156 ± 0.043
3.425LysAsn: 3.425 ± 0.056
2.372LysPro: 2.372 ± 0.045
3.241LysGln: 3.241 ± 0.056
3.438LysArg: 3.438 ± 0.056
3.536LysSer: 3.536 ± 0.06
3.686LysThr: 3.686 ± 0.051
4.961LysVal: 4.961 ± 0.064
0.835LysTrp: 0.835 ± 0.025
2.093LysTyr: 2.093 ± 0.039
0.0LysXaa: 0.0 ± 0.0
Leu
7.098LeuAla: 7.098 ± 0.079
0.56LeuCys: 0.56 ± 0.021
4.808LeuAsp: 4.808 ± 0.058
6.835LeuGlu: 6.835 ± 0.085
4.864LeuPhe: 4.864 ± 0.081
6.458LeuGly: 6.458 ± 0.074
1.794LeuHis: 1.794 ± 0.04
7.102LeuIle: 7.102 ± 0.093
6.967LeuLys: 6.967 ± 0.083
9.993LeuLeu: 9.993 ± 0.128
2.412LeuMet: 2.412 ± 0.043
4.305LeuAsn: 4.305 ± 0.061
3.973LeuPro: 3.973 ± 0.057
3.224LeuGln: 3.224 ± 0.046
3.53LeuArg: 3.53 ± 0.056
6.884LeuSer: 6.884 ± 0.069
5.32LeuThr: 5.32 ± 0.064
6.436LeuVal: 6.436 ± 0.078
0.891LeuTrp: 0.891 ± 0.025
3.061LeuTyr: 3.061 ± 0.054
0.0LeuXaa: 0.0 ± 0.0
Met
2.095MetAla: 2.095 ± 0.04
0.133MetCys: 0.133 ± 0.011
1.529MetAsp: 1.529 ± 0.035
2.274MetGlu: 2.274 ± 0.043
1.041MetPhe: 1.041 ± 0.028
1.861MetGly: 1.861 ± 0.035
0.457MetHis: 0.457 ± 0.02
2.087MetIle: 2.087 ± 0.036
2.351MetLys: 2.351 ± 0.046
2.535MetLeu: 2.535 ± 0.044
0.789MetMet: 0.789 ± 0.03
1.379MetAsn: 1.379 ± 0.029
1.07MetPro: 1.07 ± 0.035
0.813MetGln: 0.813 ± 0.025
1.012MetArg: 1.012 ± 0.027
1.446MetSer: 1.446 ± 0.033
1.423MetThr: 1.423 ± 0.034
1.858MetVal: 1.858 ± 0.042
0.198MetTrp: 0.198 ± 0.011
0.688MetTyr: 0.688 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
2.82AsnAla: 2.82 ± 0.053
0.301AsnCys: 0.301 ± 0.016
2.333AsnAsp: 2.333 ± 0.046
3.756AsnGlu: 3.756 ± 0.05
1.622AsnPhe: 1.622 ± 0.035
3.48AsnGly: 3.48 ± 0.058
1.004AsnHis: 1.004 ± 0.029
3.187AsnIle: 3.187 ± 0.047
3.14AsnLys: 3.14 ± 0.057
3.971AsnLeu: 3.971 ± 0.058
1.143AsnMet: 1.143 ± 0.027
2.238AsnAsn: 2.238 ± 0.05
2.286AsnPro: 2.286 ± 0.046
1.737AsnGln: 1.737 ± 0.036
2.243AsnArg: 2.243 ± 0.044
2.369AsnSer: 2.369 ± 0.045
2.172AsnThr: 2.172 ± 0.04
3.211AsnVal: 3.211 ± 0.055
0.566AsnTrp: 0.566 ± 0.023
1.59AsnTyr: 1.59 ± 0.041
0.0AsnXaa: 0.0 ± 0.0
Pro
2.456ProAla: 2.456 ± 0.047
0.206ProCys: 0.206 ± 0.013
2.37ProAsp: 2.37 ± 0.045
3.622ProGlu: 3.622 ± 0.057
2.086ProPhe: 2.086 ± 0.039
2.807ProGly: 2.807 ± 0.056
0.765ProHis: 0.765 ± 0.026
2.754ProIle: 2.754 ± 0.042
1.984ProLys: 1.984 ± 0.04
3.539ProLeu: 3.539 ± 0.055
0.837ProMet: 0.837 ± 0.025
1.512ProAsn: 1.512 ± 0.041
1.072ProPro: 1.072 ± 0.03
1.009ProGln: 1.009 ± 0.03
1.073ProArg: 1.073 ± 0.025
2.238ProSer: 2.238 ± 0.038
1.766ProThr: 1.766 ± 0.039
3.102ProVal: 3.102 ± 0.058
0.388ProTrp: 0.388 ± 0.016
1.526ProTyr: 1.526 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
2.372GlnAla: 2.372 ± 0.041
0.183GlnCys: 0.183 ± 0.011
1.549GlnAsp: 1.549 ± 0.035
2.887GlnGlu: 2.887 ± 0.049
1.428GlnPhe: 1.428 ± 0.033
2.051GlnGly: 2.051 ± 0.041
0.692GlnHis: 0.692 ± 0.024
2.389GlnIle: 2.389 ± 0.041
2.822GlnLys: 2.822 ± 0.051
3.556GlnLeu: 3.556 ± 0.057
1.061GlnMet: 1.061 ± 0.03
1.678GlnAsn: 1.678 ± 0.039
1.158GlnPro: 1.158 ± 0.03
1.665GlnGln: 1.665 ± 0.049
1.504GlnArg: 1.504 ± 0.038
1.983GlnSer: 1.983 ± 0.043
1.853GlnThr: 1.853 ± 0.037
2.195GlnVal: 2.195 ± 0.043
0.389GlnTrp: 0.389 ± 0.017
1.146GlnTyr: 1.146 ± 0.031
0.0GlnXaa: 0.0 ± 0.0
Arg
2.46ArgAla: 2.46 ± 0.049
0.287ArgCys: 0.287 ± 0.014
2.039ArgAsp: 2.039 ± 0.044
3.384ArgGlu: 3.384 ± 0.054
2.09ArgPhe: 2.09 ± 0.04
2.382ArgGly: 2.382 ± 0.037
0.68ArgHis: 0.68 ± 0.023
3.117ArgIle: 3.117 ± 0.049
3.389ArgLys: 3.389 ± 0.053
4.053ArgLeu: 4.053 ± 0.059
1.3ArgMet: 1.3 ± 0.034
2.01ArgAsn: 2.01 ± 0.037
1.361ArgPro: 1.361 ± 0.033
1.442ArgGln: 1.442 ± 0.036
1.853ArgArg: 1.853 ± 0.046
2.3ArgSer: 2.3 ± 0.044
2.083ArgThr: 2.083 ± 0.043
2.653ArgVal: 2.653 ± 0.045
0.45ArgTrp: 0.45 ± 0.019
1.584ArgTyr: 1.584 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
3.643SerAla: 3.643 ± 0.051
0.42SerCys: 0.42 ± 0.017
2.982SerAsp: 2.982 ± 0.054
4.15SerGlu: 4.15 ± 0.058
3.389SerPhe: 3.389 ± 0.049
4.504SerGly: 4.504 ± 0.063
1.213SerHis: 1.213 ± 0.032
4.878SerIle: 4.878 ± 0.063
3.879SerLys: 3.879 ± 0.061
6.162SerLeu: 6.162 ± 0.081
1.715SerMet: 1.715 ± 0.035
2.64SerAsn: 2.64 ± 0.047
2.287SerPro: 2.287 ± 0.04
1.78SerGln: 1.78 ± 0.035
2.422SerArg: 2.422 ± 0.047
3.95SerSer: 3.95 ± 0.056
2.923SerThr: 2.923 ± 0.043
4.271SerVal: 4.271 ± 0.058
0.701SerTrp: 0.701 ± 0.023
2.204SerTyr: 2.204 ± 0.04
0.0SerXaa: 0.0 ± 0.0
Thr
3.824ThrAla: 3.824 ± 0.06
0.336ThrCys: 0.336 ± 0.018
2.753ThrAsp: 2.753 ± 0.05
3.649ThrGlu: 3.649 ± 0.056
2.613ThrPhe: 2.613 ± 0.05
4.392ThrGly: 4.392 ± 0.062
0.985ThrHis: 0.985 ± 0.028
4.169ThrIle: 4.169 ± 0.057
3.12ThrLys: 3.12 ± 0.052
4.899ThrLeu: 4.899 ± 0.064
1.341ThrMet: 1.341 ± 0.033
2.311ThrAsn: 2.311 ± 0.045
2.191ThrPro: 2.191 ± 0.044
1.239ThrGln: 1.239 ± 0.034
1.89ThrArg: 1.89 ± 0.042
3.122ThrSer: 3.122 ± 0.049
2.688ThrThr: 2.688 ± 0.053
4.316ThrVal: 4.316 ± 0.062
0.494ThrTrp: 0.494 ± 0.019
1.873ThrTyr: 1.873 ± 0.038
0.0ThrXaa: 0.0 ± 0.0
Val
4.83ValAla: 4.83 ± 0.064
0.55ValCys: 0.55 ± 0.023
3.684ValAsp: 3.684 ± 0.058
5.028ValGlu: 5.028 ± 0.068
3.315ValPhe: 3.315 ± 0.052
4.676ValGly: 4.676 ± 0.063
1.332ValHis: 1.332 ± 0.033
5.689ValIle: 5.689 ± 0.07
5.018ValLys: 5.018 ± 0.067
6.995ValLeu: 6.995 ± 0.077
1.94ValMet: 1.94 ± 0.042
3.15ValAsn: 3.15 ± 0.052
2.843ValPro: 2.843 ± 0.048
2.26ValGln: 2.26 ± 0.042
2.61ValArg: 2.61 ± 0.046
4.739ValSer: 4.739 ± 0.054
4.215ValThr: 4.215 ± 0.055
5.04ValVal: 5.04 ± 0.074
0.643ValTrp: 0.643 ± 0.023
2.246ValTyr: 2.246 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
0.658TrpAla: 0.658 ± 0.022
0.067TrpCys: 0.067 ± 0.008
0.576TrpAsp: 0.576 ± 0.023
0.747TrpGlu: 0.747 ± 0.025
0.523TrpPhe: 0.523 ± 0.021
0.73TrpGly: 0.73 ± 0.024
0.213TrpHis: 0.213 ± 0.012
0.885TrpIle: 0.885 ± 0.024
0.848TrpLys: 0.848 ± 0.025
1.089TrpLeu: 1.089 ± 0.03
0.32TrpMet: 0.32 ± 0.018
0.57TrpAsn: 0.57 ± 0.022
0.279TrpPro: 0.279 ± 0.014
0.37TrpGln: 0.37 ± 0.017
0.408TrpArg: 0.408 ± 0.018
0.635TrpSer: 0.635 ± 0.022
0.538TrpThr: 0.538 ± 0.023
0.695TrpVal: 0.695 ± 0.022
0.15TrpTrp: 0.15 ± 0.011
0.366TrpTyr: 0.366 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.0TyrAla: 2.0 ± 0.038
0.286TyrCys: 0.286 ± 0.016
1.922TyrAsp: 1.922 ± 0.045
2.586TyrGlu: 2.586 ± 0.045
1.763TyrPhe: 1.763 ± 0.037
2.56TyrGly: 2.56 ± 0.049
0.828TyrHis: 0.828 ± 0.028
2.317TyrIle: 2.317 ± 0.04
2.219TyrLys: 2.219 ± 0.039
3.523TyrLeu: 3.523 ± 0.057
0.85TyrMet: 0.85 ± 0.024
1.622TyrAsn: 1.622 ± 0.036
1.434TyrPro: 1.434 ± 0.041
1.429TyrGln: 1.429 ± 0.032
1.668TyrArg: 1.668 ± 0.036
2.131TyrSer: 2.131 ± 0.044
1.722TyrThr: 1.722 ± 0.038
2.07TyrVal: 2.07 ± 0.039
0.431TyrTrp: 0.431 ± 0.018
1.448TyrTyr: 1.448 ± 0.036
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4545 proteins (1304964 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski