Gluconobacter wancherniae NBRC 103581

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Gluconobacter; Gluconobacter wancherniae

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2573 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A511B276|A0A511B276_9PROT AB hydrolase-1 domain-containing protein OS=Gluconobacter wancherniae NBRC 103581 OX=656744 GN=GWA01_23250 PE=4 SV=1
MM1 pKa = 7.51SIIGSVQTAVSGLNAQSHH19 pKa = 6.0AFSDD23 pKa = 4.2LSNNIANSQTVGYY36 pKa = 9.75KK37 pKa = 9.31ATTTSFADD45 pKa = 3.56YY46 pKa = 8.97VTNNSLVQDD55 pKa = 4.19GQSMSDD61 pKa = 3.12SVLATTRR68 pKa = 11.84QHH70 pKa = 6.64NDD72 pKa = 2.69NQGTVTASTNTLALAISGNGFFNVQQASGNSTGTAPVFNGQQYY115 pKa = 6.71YY116 pKa = 8.89TRR118 pKa = 11.84NGDD121 pKa = 3.64FSQDD125 pKa = 2.9ANGYY129 pKa = 9.44LKK131 pKa = 9.55NTSNYY136 pKa = 9.29YY137 pKa = 10.57LEE139 pKa = 6.16GYY141 pKa = 7.95QTDD144 pKa = 3.39SSGKK148 pKa = 9.22MSTTLAPIQIQNVAFRR164 pKa = 11.84PTQTTTMTVSSAIGSVGGTGQTSTTTATAYY194 pKa = 10.3DD195 pKa = 4.13SQGVAHH201 pKa = 7.06DD202 pKa = 4.81VSLTWTQTGTDD213 pKa = 3.12TWTVSNTADD222 pKa = 3.49PTNNTTVTFNDD233 pKa = 4.07DD234 pKa = 3.27GSLKK238 pKa = 10.45QVGNTTQATGATASFAFLGTPQNMTVSLGTIGSTSGVSLSSGTSSGATTSTMTTDD293 pKa = 2.8STTTGTYY300 pKa = 10.16SGIAIQSDD308 pKa = 4.17GSVMATFDD316 pKa = 3.54NGLSQLVAKK325 pKa = 10.47IPLTTFSNPNGLAAQDD341 pKa = 3.83GQAYY345 pKa = 6.79TATATSGSPSAPTAVNTNGAGTLDD369 pKa = 3.64VGSLEE374 pKa = 4.87SSTTDD379 pKa = 3.05LTSNLTQLIVDD390 pKa = 3.77QQAYY394 pKa = 8.88GANTKK399 pKa = 9.88VVTTANQMLQTTLAMIQQ416 pKa = 3.18

Molecular weight: 42.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A511B4H6|A0A511B4H6_9PROT ABC export transporter fused inner membrane and ATPases OS=Gluconobacter wancherniae NBRC 103581 OX=656744 GN=GWA01_23920 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.02RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.43GFRR19 pKa = 11.84TRR21 pKa = 11.84SATVGGRR28 pKa = 11.84RR29 pKa = 11.84VLANRR34 pKa = 11.84RR35 pKa = 11.84TKK37 pKa = 10.41GRR39 pKa = 11.84KK40 pKa = 8.93KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2573

0

2573

835224

40

2858

324.6

35.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.122 ± 0.053

0.96 ± 0.014

5.463 ± 0.035

5.372 ± 0.045

3.575 ± 0.031

8.367 ± 0.043

2.446 ± 0.02

5.315 ± 0.036

3.07 ± 0.029

10.385 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.562 ± 0.022

3.021 ± 0.038

5.274 ± 0.034

3.555 ± 0.03

6.804 ± 0.045

6.345 ± 0.037

5.718 ± 0.039

7.076 ± 0.04

1.333 ± 0.019

2.237 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski