Thauera linaloolentis 47Lol = DSM 12138

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae; Thauera; Thauera linaloolentis

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3836 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|N6Y8D0|N6Y8D0_9RHOO Glyoxalase/bleomycin resistance protein/dioxygen OS=Thauera linaloolentis 47Lol = DSM 12138 OX=1123367 GN=C666_10170 PE=3 SV=1
MM1 pKa = 6.94SAEE4 pKa = 4.09VATPDD9 pKa = 3.22ILVFTDD15 pKa = 3.65SAADD19 pKa = 3.49KK20 pKa = 10.57VRR22 pKa = 11.84EE23 pKa = 4.24LIEE26 pKa = 4.39EE27 pKa = 4.19EE28 pKa = 4.44GNPEE32 pKa = 3.6LKK34 pKa = 10.72LRR36 pKa = 11.84VFVSGGGCSGFQYY49 pKa = 10.93GFTFDD54 pKa = 4.28EE55 pKa = 4.93EE56 pKa = 4.59VNEE59 pKa = 5.57DD60 pKa = 3.3DD61 pKa = 3.6TTYY64 pKa = 11.03EE65 pKa = 4.12KK66 pKa = 11.07NGVMLLIDD74 pKa = 3.79SMSYY78 pKa = 10.23QYY80 pKa = 11.55LVGAEE85 pKa = 3.64IDD87 pKa = 3.75YY88 pKa = 10.77TEE90 pKa = 4.43GLEE93 pKa = 4.25GSQFVIRR100 pKa = 11.84NPNATSTCGCGSSFSAA116 pKa = 4.79

Molecular weight: 12.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|N6XSB6|N6XSB6_9RHOO Beta-lactamase OS=Thauera linaloolentis 47Lol = DSM 12138 OX=1123367 GN=C666_16955 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.6RR14 pKa = 11.84THH16 pKa = 5.75GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.91GRR39 pKa = 11.84HH40 pKa = 4.92RR41 pKa = 11.84LAVV44 pKa = 3.37

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3836

0

3836

1253860

23

5559

326.9

35.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.04 ± 0.06

0.928 ± 0.015

5.609 ± 0.034

5.696 ± 0.036

3.545 ± 0.026

8.692 ± 0.041

2.323 ± 0.021

4.589 ± 0.026

2.821 ± 0.032

10.952 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.302 ± 0.022

2.526 ± 0.028

5.057 ± 0.032

3.489 ± 0.024

7.632 ± 0.041

5.114 ± 0.035

4.779 ± 0.041

7.218 ± 0.04

1.4 ± 0.017

2.288 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski