Novosphingobium sp. 9U

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium; unclassified Novosphingobium

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4782 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A653J8T0|A0A653J8T0_9SPHN Quinohemoprotein alcohol dehydrogenase ADH IIB OS=Novosphingobium sp. 9U OX=2653158 GN=qbdA PE=3 SV=1
MM1 pKa = 7.33TRR3 pKa = 11.84TFTSILALAALALPQAAPAATLLTNSDD30 pKa = 3.09GGYY33 pKa = 10.44GQIALPKK40 pKa = 10.02NDD42 pKa = 4.45DD43 pKa = 3.96SSSGAYY49 pKa = 9.39IMPFSIDD56 pKa = 3.56FFGSVYY62 pKa = 11.03DD63 pKa = 3.96EE64 pKa = 4.89LFVNNNGNVTFSTGLGSFTPFDD86 pKa = 4.35FGSLGQAMIAPFWADD101 pKa = 2.29VDD103 pKa = 4.4TRR105 pKa = 11.84CVSCGNVYY113 pKa = 10.18IGSFAPGQINVTWDD127 pKa = 3.11NVGYY131 pKa = 10.41YY132 pKa = 9.5SQHH135 pKa = 5.78SDD137 pKa = 3.19KK138 pKa = 11.43LNSFQLNLYY147 pKa = 9.97QSGNAGDD154 pKa = 3.99FDD156 pKa = 4.39IEE158 pKa = 4.66FRR160 pKa = 11.84YY161 pKa = 10.43ADD163 pKa = 4.29LNWTTGDD170 pKa = 3.2ASQGVNGLGGVPAAAGYY187 pKa = 8.75TNGAGATQLQPGSFLSPGALSFTDD211 pKa = 3.45SSNVGEE217 pKa = 4.51DD218 pKa = 3.45GRR220 pKa = 11.84WVYY223 pKa = 10.87NIRR226 pKa = 11.84NDD228 pKa = 4.99APPTDD233 pKa = 4.19YY234 pKa = 10.95GASPEE239 pKa = 4.19NPVLPTEE246 pKa = 4.04QTPGGGYY253 pKa = 10.41EE254 pKa = 4.08FTFEE258 pKa = 4.32IDD260 pKa = 3.1PTRR263 pKa = 11.84PTFIDD268 pKa = 3.82PPVATGYY275 pKa = 11.12DD276 pKa = 4.13FGAEE280 pKa = 3.83GGALFTSAVFTSLLNDD296 pKa = 3.17SDD298 pKa = 5.54GYY300 pKa = 10.57QLYY303 pKa = 10.89DD304 pKa = 3.31GANLLGQVAIGQTFTFASPLAAFQLRR330 pKa = 11.84GIDD333 pKa = 4.48SINMLQPGDD342 pKa = 3.74PLAFVSGFTFDD353 pKa = 2.9RR354 pKa = 11.84TGTVTVTQNPYY365 pKa = 9.12VQDD368 pKa = 3.31VAAAVPEE375 pKa = 4.23TATWAMMILGIGMVGGGLRR394 pKa = 11.84RR395 pKa = 11.84RR396 pKa = 11.84PPRR399 pKa = 11.84ARR401 pKa = 11.84FAA403 pKa = 4.07

Molecular weight: 42.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A653JB82|A0A653JB82_9SPHN Outer membrane-specific lipoprotein transporter subunit ATP-binding component of ABC superfamily OS=Novosphingobium sp. 9U OX=2653158 GN=lolD PE=4 SV=1
MM1 pKa = 7.56PRR3 pKa = 11.84PRR5 pKa = 11.84EE6 pKa = 3.7PASPFRR12 pKa = 11.84YY13 pKa = 9.43FISSPEE19 pKa = 4.07VIRR22 pKa = 11.84LVVLMYY28 pKa = 10.91VRR30 pKa = 11.84FPLSLRR36 pKa = 11.84NVEE39 pKa = 4.48DD40 pKa = 3.88LPFEE44 pKa = 4.39RR45 pKa = 11.84GIDD48 pKa = 3.48ICHH51 pKa = 5.07EE52 pKa = 4.15TARR55 pKa = 11.84LRR57 pKa = 11.84WNRR60 pKa = 11.84FGPMFAGEE68 pKa = 4.19VQRR71 pKa = 11.84KK72 pKa = 7.8RR73 pKa = 11.84VCRR76 pKa = 11.84MRR78 pKa = 11.84GLRR81 pKa = 11.84HH82 pKa = 5.56WRR84 pKa = 11.84WHH86 pKa = 5.88LDD88 pKa = 3.16QRR90 pKa = 11.84YY91 pKa = 9.54AKK93 pKa = 10.62LNDD96 pKa = 3.04EE97 pKa = 4.57MVYY100 pKa = 10.17LWRR103 pKa = 11.84AVDD106 pKa = 3.72HH107 pKa = 6.42EE108 pKa = 4.84GEE110 pKa = 4.26VLEE113 pKa = 4.63SYY115 pKa = 10.31IIKK118 pKa = 9.2TRR120 pKa = 11.84DD121 pKa = 2.87KK122 pKa = 10.87AAALSFMKK130 pKa = 10.44KK131 pKa = 9.41ALKK134 pKa = 10.28RR135 pKa = 11.84HH136 pKa = 6.1GSPEE140 pKa = 4.21TITTDD145 pKa = 3.93GLRR148 pKa = 11.84SCCAAMNEE156 pKa = 4.59LGNCEE161 pKa = 3.94KK162 pKa = 10.96QEE164 pKa = 3.91VGRR167 pKa = 11.84RR168 pKa = 11.84ANNRR172 pKa = 11.84VEE174 pKa = 4.38NSHH177 pKa = 6.79LPFRR181 pKa = 11.84RR182 pKa = 11.84RR183 pKa = 11.84KK184 pKa = 9.39SAMLRR189 pKa = 11.84FRR191 pKa = 11.84RR192 pKa = 11.84VKK194 pKa = 9.6TLQKK198 pKa = 10.08FASVHH203 pKa = 5.88ANIHH207 pKa = 5.04NHH209 pKa = 5.43FQPGTSPSQPRR220 pKa = 11.84DD221 pKa = 3.48LQGTPLSCLGG231 pKa = 3.65

Molecular weight: 27.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4782

0

4782

1383869

21

2345

289.4

31.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.052 ± 0.058

0.92 ± 0.013

5.702 ± 0.025

5.531 ± 0.034

3.463 ± 0.022

8.793 ± 0.041

2.086 ± 0.022

4.661 ± 0.026

2.994 ± 0.028

9.953 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.396 ± 0.02

2.522 ± 0.023

5.345 ± 0.026

3.353 ± 0.02

7.369 ± 0.041

5.542 ± 0.027

5.324 ± 0.031

7.296 ± 0.03

1.45 ± 0.015

2.247 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski