Amino acid dipepetide frequency for Mycobacterium sp. EPa45

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.468AlaAla: 19.468 ± 0.17
1.007AlaCys: 1.007 ± 0.023
8.477AlaAsp: 8.477 ± 0.073
7.671AlaGlu: 7.671 ± 0.08
3.52AlaPhe: 3.52 ± 0.046
11.682AlaGly: 11.682 ± 0.093
2.486AlaHis: 2.486 ± 0.042
5.5AlaIle: 5.5 ± 0.064
3.128AlaLys: 3.128 ± 0.057
12.799AlaLeu: 12.799 ± 0.104
2.975AlaMet: 2.975 ± 0.05
2.791AlaAsn: 2.791 ± 0.041
6.223AlaPro: 6.223 ± 0.078
3.917AlaGln: 3.917 ± 0.045
8.049AlaArg: 8.049 ± 0.076
5.893AlaSer: 5.893 ± 0.057
7.155AlaThr: 7.155 ± 0.072
11.626AlaVal: 11.626 ± 0.108
1.616AlaTrp: 1.616 ± 0.032
2.382AlaTyr: 2.382 ± 0.035
0.0AlaXaa: 0.0 ± 0.0
Cys
1.061CysAla: 1.061 ± 0.027
0.077CysCys: 0.077 ± 0.008
0.54CysAsp: 0.54 ± 0.018
0.419CysGlu: 0.419 ± 0.016
0.232CysPhe: 0.232 ± 0.012
0.952CysGly: 0.952 ± 0.024
0.18CysHis: 0.18 ± 0.009
0.275CysIle: 0.275 ± 0.011
0.128CysLys: 0.128 ± 0.008
0.635CysLeu: 0.635 ± 0.016
0.122CysMet: 0.122 ± 0.009
0.183CysAsn: 0.183 ± 0.01
0.497CysPro: 0.497 ± 0.017
0.221CysGln: 0.221 ± 0.01
0.594CysArg: 0.594 ± 0.022
0.484CysSer: 0.484 ± 0.018
0.488CysThr: 0.488 ± 0.017
0.657CysVal: 0.657 ± 0.023
0.13CysTrp: 0.13 ± 0.008
0.213CysTyr: 0.213 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
7.597AspAla: 7.597 ± 0.081
0.463AspCys: 0.463 ± 0.016
4.387AspAsp: 4.387 ± 0.063
4.052AspGlu: 4.052 ± 0.047
1.864AspPhe: 1.864 ± 0.031
5.957AspGly: 5.957 ± 0.071
1.426AspHis: 1.426 ± 0.028
2.804AspIle: 2.804 ± 0.039
1.384AspLys: 1.384 ± 0.032
6.012AspLeu: 6.012 ± 0.061
1.029AspMet: 1.029 ± 0.027
1.371AspAsn: 1.371 ± 0.027
4.401AspPro: 4.401 ± 0.048
1.805AspGln: 1.805 ± 0.03
4.44AspArg: 4.44 ± 0.051
2.945AspSer: 2.945 ± 0.039
3.172AspThr: 3.172 ± 0.044
5.546AspVal: 5.546 ± 0.054
1.018AspTrp: 1.018 ± 0.025
1.421AspTyr: 1.421 ± 0.029
0.0AspXaa: 0.0 ± 0.0
Glu
5.963GluAla: 5.963 ± 0.072
0.379GluCys: 0.379 ± 0.015
2.478GluAsp: 2.478 ± 0.04
2.456GluGlu: 2.456 ± 0.045
1.87GluPhe: 1.87 ± 0.033
3.224GluGly: 3.224 ± 0.049
1.512GluHis: 1.512 ± 0.032
2.678GluIle: 2.678 ± 0.041
1.408GluLys: 1.408 ± 0.034
6.543GluLeu: 6.543 ± 0.074
1.111GluMet: 1.111 ± 0.026
1.127GluAsn: 1.127 ± 0.025
2.983GluPro: 2.983 ± 0.039
2.185GluGln: 2.185 ± 0.04
4.231GluArg: 4.231 ± 0.056
2.695GluSer: 2.695 ± 0.038
2.579GluThr: 2.579 ± 0.038
4.387GluVal: 4.387 ± 0.055
0.736GluTrp: 0.736 ± 0.021
1.128GluTyr: 1.128 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
4.044PheAla: 4.044 ± 0.052
0.329PheCys: 0.329 ± 0.014
2.474PheAsp: 2.474 ± 0.034
1.579PheGlu: 1.579 ± 0.029
1.042PhePhe: 1.042 ± 0.027
3.609PheGly: 3.609 ± 0.048
0.644PheHis: 0.644 ± 0.019
1.209PheIle: 1.209 ± 0.028
0.585PheLys: 0.585 ± 0.017
2.605PheLeu: 2.605 ± 0.046
0.525PheMet: 0.525 ± 0.018
0.842PheAsn: 0.842 ± 0.024
1.473PhePro: 1.473 ± 0.026
0.693PheGln: 0.693 ± 0.019
1.697PheArg: 1.697 ± 0.034
1.79PheSer: 1.79 ± 0.036
2.178PheThr: 2.178 ± 0.036
2.638PheVal: 2.638 ± 0.044
0.467PheTrp: 0.467 ± 0.019
0.753PheTyr: 0.753 ± 0.023
0.0PheXaa: 0.0 ± 0.0
Gly
10.041GlyAla: 10.041 ± 0.086
0.787GlyCys: 0.787 ± 0.024
5.017GlyAsp: 5.017 ± 0.06
4.318GlyGlu: 4.318 ± 0.056
3.14GlyPhe: 3.14 ± 0.043
7.897GlyGly: 7.897 ± 0.118
2.058GlyHis: 2.058 ± 0.035
4.273GlyIle: 4.273 ± 0.052
2.309GlyLys: 2.309 ± 0.037
8.679GlyLeu: 8.679 ± 0.071
2.131GlyMet: 2.131 ± 0.042
2.044GlyAsn: 2.044 ± 0.043
4.611GlyPro: 4.611 ± 0.054
2.945GlyGln: 2.945 ± 0.044
6.101GlyArg: 6.101 ± 0.064
5.273GlySer: 5.273 ± 0.059
5.302GlyThr: 5.302 ± 0.06
7.686GlyVal: 7.686 ± 0.083
1.668GlyTrp: 1.668 ± 0.035
2.44GlyTyr: 2.44 ± 0.035
0.0GlyXaa: 0.0 ± 0.0
His
2.415HisAla: 2.415 ± 0.038
0.219HisCys: 0.219 ± 0.013
1.339HisAsp: 1.339 ± 0.033
1.076HisGlu: 1.076 ± 0.027
0.713HisPhe: 0.713 ± 0.019
2.071HisGly: 2.071 ± 0.03
0.671HisHis: 0.671 ± 0.021
0.905HisIle: 0.905 ± 0.024
0.374HisLys: 0.374 ± 0.015
2.095HisLeu: 2.095 ± 0.036
0.373HisMet: 0.373 ± 0.013
0.504HisAsn: 0.504 ± 0.015
1.619HisPro: 1.619 ± 0.029
0.656HisGln: 0.656 ± 0.022
1.771HisArg: 1.771 ± 0.032
1.146HisSer: 1.146 ± 0.028
1.249HisThr: 1.249 ± 0.026
1.718HisVal: 1.718 ± 0.031
0.417HisTrp: 0.417 ± 0.014
0.579HisTyr: 0.579 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
6.608IleAla: 6.608 ± 0.055
0.402IleCys: 0.402 ± 0.016
3.466IleAsp: 3.466 ± 0.05
2.653IleGlu: 2.653 ± 0.039
1.142IlePhe: 1.142 ± 0.027
4.658IleGly: 4.658 ± 0.046
0.85IleHis: 0.85 ± 0.022
1.595IleIle: 1.595 ± 0.033
1.01IleLys: 1.01 ± 0.028
3.22IleLeu: 3.22 ± 0.047
0.633IleMet: 0.633 ± 0.017
1.243IleAsn: 1.243 ± 0.03
2.55IlePro: 2.55 ± 0.04
1.0IleGln: 1.0 ± 0.025
2.718IleArg: 2.718 ± 0.044
2.442IleSer: 2.442 ± 0.036
3.017IleThr: 3.017 ± 0.041
3.841IleVal: 3.841 ± 0.049
0.501IleTrp: 0.501 ± 0.016
0.84IleTyr: 0.84 ± 0.023
0.0IleXaa: 0.0 ± 0.0
Lys
2.841LysAla: 2.841 ± 0.047
0.104LysCys: 0.104 ± 0.008
1.194LysAsp: 1.194 ± 0.027
0.981LysGlu: 0.981 ± 0.027
0.684LysPhe: 0.684 ± 0.017
1.614LysGly: 1.614 ± 0.037
0.518LysHis: 0.518 ± 0.016
1.088LysIle: 1.088 ± 0.025
0.689LysLys: 0.689 ± 0.025
2.34LysLeu: 2.34 ± 0.04
0.516LysMet: 0.516 ± 0.015
0.556LysAsn: 0.556 ± 0.021
1.55LysPro: 1.55 ± 0.033
0.749LysGln: 0.749 ± 0.026
1.652LysArg: 1.652 ± 0.028
1.318LysSer: 1.318 ± 0.028
1.391LysThr: 1.391 ± 0.026
1.989LysVal: 1.989 ± 0.035
0.334LysTrp: 0.334 ± 0.013
0.532LysTyr: 0.532 ± 0.019
0.0LysXaa: 0.0 ± 0.0
Leu
13.966LeuAla: 13.966 ± 0.114
0.836LeuCys: 0.836 ± 0.021
6.415LeuAsp: 6.415 ± 0.072
4.361LeuGlu: 4.361 ± 0.054
2.735LeuPhe: 2.735 ± 0.043
8.877LeuGly: 8.877 ± 0.067
2.057LeuHis: 2.057 ± 0.036
4.313LeuIle: 4.313 ± 0.058
1.976LeuLys: 1.976 ± 0.034
9.581LeuLeu: 9.581 ± 0.098
1.803LeuMet: 1.803 ± 0.034
2.319LeuAsn: 2.319 ± 0.035
5.765LeuPro: 5.765 ± 0.062
2.604LeuGln: 2.604 ± 0.039
7.293LeuArg: 7.293 ± 0.068
5.786LeuSer: 5.786 ± 0.064
6.449LeuThr: 6.449 ± 0.06
8.377LeuVal: 8.377 ± 0.077
1.195LeuTrp: 1.195 ± 0.027
1.682LeuTyr: 1.682 ± 0.03
0.0LeuXaa: 0.0 ± 0.0
Met
2.689MetAla: 2.689 ± 0.043
0.202MetCys: 0.202 ± 0.011
0.925MetAsp: 0.925 ± 0.024
0.713MetGlu: 0.713 ± 0.016
0.663MetPhe: 0.663 ± 0.018
1.521MetGly: 1.521 ± 0.033
0.431MetHis: 0.431 ± 0.015
0.936MetIle: 0.936 ± 0.026
0.499MetLys: 0.499 ± 0.014
2.075MetLeu: 2.075 ± 0.036
0.454MetMet: 0.454 ± 0.016
0.53MetAsn: 0.53 ± 0.016
1.261MetPro: 1.261 ± 0.031
0.568MetGln: 0.568 ± 0.019
1.526MetArg: 1.526 ± 0.031
1.603MetSer: 1.603 ± 0.028
1.89MetThr: 1.89 ± 0.033
1.633MetVal: 1.633 ± 0.028
0.271MetTrp: 0.271 ± 0.012
0.357MetTyr: 0.357 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
2.836AsnAla: 2.836 ± 0.038
0.205AsnCys: 0.205 ± 0.012
1.324AsnAsp: 1.324 ± 0.031
1.033AsnGlu: 1.033 ± 0.022
0.737AsnPhe: 0.737 ± 0.023
2.234AsnGly: 2.234 ± 0.044
0.47AsnHis: 0.47 ± 0.017
0.987AsnIle: 0.987 ± 0.025
0.506AsnLys: 0.506 ± 0.018
2.249AsnLeu: 2.249 ± 0.034
0.441AsnMet: 0.441 ± 0.016
0.64AsnAsn: 0.64 ± 0.019
1.896AsnPro: 1.896 ± 0.038
0.706AsnGln: 0.706 ± 0.02
1.605AsnArg: 1.605 ± 0.026
1.287AsnSer: 1.287 ± 0.029
1.4AsnThr: 1.4 ± 0.026
1.856AsnVal: 1.856 ± 0.033
0.424AsnTrp: 0.424 ± 0.015
0.621AsnTyr: 0.621 ± 0.019
0.0AsnXaa: 0.0 ± 0.0
Pro
7.27ProAla: 7.27 ± 0.074
0.281ProCys: 0.281 ± 0.013
4.402ProAsp: 4.402 ± 0.053
3.599ProGlu: 3.599 ± 0.048
1.667ProPhe: 1.667 ± 0.028
5.626ProGly: 5.626 ± 0.06
1.229ProHis: 1.229 ± 0.027
2.289ProIle: 2.289 ± 0.034
1.352ProLys: 1.352 ± 0.028
4.943ProLeu: 4.943 ± 0.054
1.251ProMet: 1.251 ± 0.026
1.366ProAsn: 1.366 ± 0.03
3.695ProPro: 3.695 ± 0.087
1.877ProGln: 1.877 ± 0.035
3.228ProArg: 3.228 ± 0.041
3.112ProSer: 3.112 ± 0.043
3.493ProThr: 3.493 ± 0.054
5.09ProVal: 5.09 ± 0.069
0.851ProTrp: 0.851 ± 0.022
1.255ProTyr: 1.255 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
3.63GlnAla: 3.63 ± 0.056
0.236GlnCys: 0.236 ± 0.01
1.245GlnAsp: 1.245 ± 0.027
1.065GlnGlu: 1.065 ± 0.028
1.006GlnPhe: 1.006 ± 0.023
2.085GlnGly: 2.085 ± 0.037
0.751GlnHis: 0.751 ± 0.02
1.576GlnIle: 1.576 ± 0.03
0.681GlnLys: 0.681 ± 0.019
3.642GlnLeu: 3.642 ± 0.054
0.705GlnMet: 0.705 ± 0.02
0.667GlnAsn: 0.667 ± 0.019
1.92GlnPro: 1.92 ± 0.035
1.274GlnGln: 1.274 ± 0.028
2.749GlnArg: 2.749 ± 0.045
1.489GlnSer: 1.489 ± 0.031
1.644GlnThr: 1.644 ± 0.029
2.505GlnVal: 2.505 ± 0.043
0.598GlnTrp: 0.598 ± 0.019
0.684GlnTyr: 0.684 ± 0.021
0.0GlnXaa: 0.0 ± 0.0
Arg
7.766ArgAla: 7.766 ± 0.085
0.568ArgCys: 0.568 ± 0.019
4.206ArgAsp: 4.206 ± 0.051
3.7ArgGlu: 3.7 ± 0.057
2.402ArgPhe: 2.402 ± 0.038
5.001ArgGly: 5.001 ± 0.056
1.696ArgHis: 1.696 ± 0.03
3.379ArgIle: 3.379 ± 0.049
1.548ArgLys: 1.548 ± 0.03
7.262ArgLeu: 7.262 ± 0.074
1.671ArgMet: 1.671 ± 0.032
1.67ArgAsn: 1.67 ± 0.031
3.805ArgPro: 3.805 ± 0.054
2.33ArgGln: 2.33 ± 0.035
6.138ArgArg: 6.138 ± 0.081
3.837ArgSer: 3.837 ± 0.047
4.122ArgThr: 4.122 ± 0.046
5.433ArgVal: 5.433 ± 0.058
1.341ArgTrp: 1.341 ± 0.025
1.904ArgTyr: 1.904 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
7.026SerAla: 7.026 ± 0.07
0.43SerCys: 0.43 ± 0.015
3.359SerAsp: 3.359 ± 0.05
2.578SerGlu: 2.578 ± 0.037
1.75SerPhe: 1.75 ± 0.036
5.613SerGly: 5.613 ± 0.055
1.065SerHis: 1.065 ± 0.025
2.276SerIle: 2.276 ± 0.037
1.172SerLys: 1.172 ± 0.028
4.922SerLeu: 4.922 ± 0.051
1.437SerMet: 1.437 ± 0.027
1.2SerAsn: 1.2 ± 0.027
3.084SerPro: 3.084 ± 0.042
1.528SerGln: 1.528 ± 0.031
3.643SerArg: 3.643 ± 0.048
3.338SerSer: 3.338 ± 0.058
3.403SerThr: 3.403 ± 0.048
4.613SerVal: 4.613 ± 0.055
0.926SerTrp: 0.926 ± 0.025
1.268SerTyr: 1.268 ± 0.028
0.0SerXaa: 0.0 ± 0.0
Thr
7.83ThrAla: 7.83 ± 0.076
0.406ThrCys: 0.406 ± 0.017
3.8ThrAsp: 3.8 ± 0.051
3.148ThrGlu: 3.148 ± 0.04
1.929ThrPhe: 1.929 ± 0.035
5.923ThrGly: 5.923 ± 0.056
1.124ThrHis: 1.124 ± 0.026
2.623ThrIle: 2.623 ± 0.035
1.367ThrLys: 1.367 ± 0.03
5.687ThrLeu: 5.687 ± 0.06
1.241ThrMet: 1.241 ± 0.029
1.283ThrAsn: 1.283 ± 0.026
3.758ThrPro: 3.758 ± 0.049
1.523ThrGln: 1.523 ± 0.028
3.567ThrArg: 3.567 ± 0.046
3.285ThrSer: 3.285 ± 0.051
4.043ThrThr: 4.043 ± 0.071
5.956ThrVal: 5.956 ± 0.07
0.872ThrTrp: 0.872 ± 0.022
1.388ThrTyr: 1.388 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
11.382ValAla: 11.382 ± 0.111
0.741ValCys: 0.741 ± 0.019
5.801ValAsp: 5.801 ± 0.057
4.496ValGlu: 4.496 ± 0.055
2.724ValPhe: 2.724 ± 0.041
7.059ValGly: 7.059 ± 0.071
1.864ValHis: 1.864 ± 0.03
4.242ValIle: 4.242 ± 0.045
1.778ValLys: 1.778 ± 0.036
8.934ValLeu: 8.934 ± 0.078
1.685ValMet: 1.685 ± 0.03
2.182ValAsn: 2.182 ± 0.039
4.589ValPro: 4.589 ± 0.054
2.175ValGln: 2.175 ± 0.043
5.722ValArg: 5.722 ± 0.06
4.763ValSer: 4.763 ± 0.055
5.549ValThr: 5.549 ± 0.06
8.679ValVal: 8.679 ± 0.097
1.095ValTrp: 1.095 ± 0.027
1.586ValTyr: 1.586 ± 0.031
0.0ValXaa: 0.0 ± 0.0
Trp
1.554TrpAla: 1.554 ± 0.032
0.172TrpCys: 0.172 ± 0.01
0.802TrpAsp: 0.802 ± 0.022
0.672TrpGlu: 0.672 ± 0.023
0.522TrpPhe: 0.522 ± 0.018
1.048TrpGly: 1.048 ± 0.025
0.362TrpHis: 0.362 ± 0.016
0.675TrpIle: 0.675 ± 0.017
0.348TrpLys: 0.348 ± 0.013
1.784TrpLeu: 1.784 ± 0.035
0.341TrpMet: 0.341 ± 0.013
0.419TrpAsn: 0.419 ± 0.016
0.859TrpPro: 0.859 ± 0.02
0.667TrpGln: 0.667 ± 0.018
1.23TrpArg: 1.23 ± 0.03
0.941TrpSer: 0.941 ± 0.024
0.944TrpThr: 0.944 ± 0.021
1.145TrpVal: 1.145 ± 0.026
0.348TrpTrp: 0.348 ± 0.015
0.351TrpTyr: 0.351 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.377TyrAla: 2.377 ± 0.036
0.245TyrCys: 0.245 ± 0.011
1.443TyrAsp: 1.443 ± 0.026
1.103TyrGlu: 1.103 ± 0.023
0.833TyrPhe: 0.833 ± 0.023
2.073TyrGly: 2.073 ± 0.036
0.515TyrHis: 0.515 ± 0.016
0.764TyrIle: 0.764 ± 0.023
0.4TyrLys: 0.4 ± 0.017
2.381TyrLeu: 2.381 ± 0.038
0.302TyrMet: 0.302 ± 0.013
0.518TyrAsn: 0.518 ± 0.018
1.336TyrPro: 1.336 ± 0.031
0.729TyrGln: 0.729 ± 0.017
1.825TyrArg: 1.825 ± 0.037
1.204TyrSer: 1.204 ± 0.027
1.288TyrThr: 1.288 ± 0.027
1.672TyrVal: 1.672 ± 0.032
0.377TyrTrp: 0.377 ± 0.016
0.545TyrTyr: 0.545 ± 0.018
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5595 proteins (1821256 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski