Candidatus Methanomethylophilus sp. 1R26

Taxonomy: cellular organisms; Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Methanomassiliicoccales; Candidatus Methanomethylophilaceae; Candidatus Methanomethylophilus; unclassified Candidatus Methanomethylophilus

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1473 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0W7THA7|A0A0W7THA7_9ARCH Phosphoesterase OS=Candidatus Methanomethylophilus sp. 1R26 OX=1769296 GN=AUQ37_03250 PE=4 SV=1
MM1 pKa = 7.9ADD3 pKa = 3.07QAVTYY8 pKa = 10.72GEE10 pKa = 4.13NTKK13 pKa = 10.8LSANAFSKK21 pKa = 10.66DD22 pKa = 3.27GYY24 pKa = 11.49SFGGWMFKK32 pKa = 10.85DD33 pKa = 3.56VVFADD38 pKa = 3.54EE39 pKa = 5.48AYY41 pKa = 9.77ILNLTSEE48 pKa = 4.7ADD50 pKa = 3.39AEE52 pKa = 4.46LTFTAVWIVNSYY64 pKa = 9.34TISFVSDD71 pKa = 3.65DD72 pKa = 4.09PVSGTTEE79 pKa = 3.76MQRR82 pKa = 11.84FTVEE86 pKa = 3.83DD87 pKa = 3.32EE88 pKa = 4.96GYY90 pKa = 8.48ATAVLSLNGFQRR102 pKa = 11.84AGYY105 pKa = 10.05SFAGWSDD112 pKa = 3.35GNRR115 pKa = 11.84TYY117 pKa = 11.57SDD119 pKa = 3.39GSLISTIDD127 pKa = 3.27LSKK130 pKa = 10.89AVSEE134 pKa = 4.84DD135 pKa = 3.37GVLTIALTAVWTANSYY151 pKa = 9.86TIFFDD156 pKa = 3.89TNGGSAIDD164 pKa = 5.3SIVADD169 pKa = 4.63CGTAIAVPEE178 pKa = 4.14PTKK181 pKa = 10.59EE182 pKa = 4.24GYY184 pKa = 8.11TFAGWLKK191 pKa = 10.88DD192 pKa = 3.18GAAYY196 pKa = 9.93SVPEE200 pKa = 4.11TMPAGDD206 pKa = 4.27LTLKK210 pKa = 10.79ASWKK214 pKa = 10.08LYY216 pKa = 10.1IPAPVNGVISLNAEE230 pKa = 3.63TDD232 pKa = 3.64GNLTLTDD239 pKa = 3.86EE240 pKa = 5.17SIGTLKK246 pKa = 10.67SYY248 pKa = 11.03ASSDD252 pKa = 3.66SAFLRR257 pKa = 11.84VSMGDD262 pKa = 3.15STFTFDD268 pKa = 3.97SKK270 pKa = 11.49AVAALNAGTLTIRR283 pKa = 11.84AMEE286 pKa = 4.32NLDD289 pKa = 4.55DD290 pKa = 3.85EE291 pKa = 4.86TAALTKK297 pKa = 10.5DD298 pKa = 3.09AAVYY302 pKa = 10.37AVGFGSNTYY311 pKa = 10.81LNGGTVTVTVPYY323 pKa = 9.1TLKK326 pKa = 10.77DD327 pKa = 3.52GQSADD332 pKa = 3.51DD333 pKa = 3.62VSIYY337 pKa = 10.24RR338 pKa = 11.84ISDD341 pKa = 3.28GSVAEE346 pKa = 4.5TIEE349 pKa = 4.05AQYY352 pKa = 11.53SDD354 pKa = 3.65GTVTFATGNLSSLYY368 pKa = 10.48AVGHH372 pKa = 6.98IDD374 pKa = 3.82TGSDD378 pKa = 4.1DD379 pKa = 4.92EE380 pKa = 6.01DD381 pKa = 5.41DD382 pKa = 3.87STAIMAVALAAVAVCLVAGIVYY404 pKa = 9.98AYY406 pKa = 9.94SRR408 pKa = 11.84RR409 pKa = 11.84VV410 pKa = 2.96

Molecular weight: 43.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0W7TJX8|A0A0W7TJX8_9ARCH Protein translation factor SUI1 homolog OS=Candidatus Methanomethylophilus sp. 1R26 OX=1769296 GN=AUQ37_05690 PE=3 SV=1
MM1 pKa = 7.46KK2 pKa = 9.88PKK4 pKa = 10.03KK5 pKa = 10.17QYY7 pKa = 10.21GRR9 pKa = 11.84QVGCDD14 pKa = 2.55RR15 pKa = 11.84CGRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84GIIRR24 pKa = 11.84RR25 pKa = 11.84YY26 pKa = 10.09GMHH29 pKa = 7.66LCRR32 pKa = 11.84QCFRR36 pKa = 11.84DD37 pKa = 3.69MAPEE41 pKa = 4.41LGFKK45 pKa = 10.27KK46 pKa = 10.81YY47 pKa = 10.16SS48 pKa = 3.24

Molecular weight: 5.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1473

0

1473

376912

43

2263

255.9

28.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.548 ± 0.091

1.744 ± 0.034

6.619 ± 0.054

6.455 ± 0.073

3.661 ± 0.046

8.447 ± 0.065

1.535 ± 0.026

6.309 ± 0.059

5.049 ± 0.065

7.907 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.326 ± 0.046

2.996 ± 0.045

4.145 ± 0.044

2.093 ± 0.032

5.965 ± 0.085

7.106 ± 0.093

5.273 ± 0.084

7.318 ± 0.056

1.039 ± 0.025

3.465 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski