Amino acid dipepetide frequency for Clostridium botulinum C phage (Clostridium botulinum C bacteriophage)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.02AlaAla: 0.02 ± 0.019
0.424AlaCys: 0.424 ± 0.091
1.718AlaAsp: 1.718 ± 0.191
2.102AlaGlu: 2.102 ± 0.234
1.435AlaPhe: 1.435 ± 0.142
1.536AlaGly: 1.536 ± 0.157
0.707AlaHis: 0.707 ± 0.118
3.941AlaIle: 3.941 ± 0.266
4.264AlaLys: 4.264 ± 0.416
3.577AlaLeu: 3.577 ± 0.295
0.869AlaMet: 0.869 ± 0.139
2.465AlaAsn: 2.465 ± 0.26
0.748AlaPro: 0.748 ± 0.155
1.637AlaGln: 1.637 ± 0.269
1.314AlaArg: 1.314 ± 0.168
2.142AlaSer: 2.142 ± 0.214
2.223AlaThr: 2.223 ± 0.244
1.455AlaVal: 1.455 ± 0.179
0.384AlaTrp: 0.384 ± 0.11
1.738AlaTyr: 1.738 ± 0.166
0.0AlaXaa: 0.0 ± 0.0
Cys
0.243CysAla: 0.243 ± 0.074
0.323CysCys: 0.323 ± 0.083
0.869CysAsp: 0.869 ± 0.151
0.829CysGlu: 0.829 ± 0.139
0.626CysPhe: 0.626 ± 0.121
1.273CysGly: 1.273 ± 0.202
0.283CysHis: 0.283 ± 0.07
1.536CysIle: 1.536 ± 0.175
1.879CysLys: 1.879 ± 0.229
0.647CysLeu: 0.647 ± 0.113
0.364CysMet: 0.364 ± 0.094
0.869CysAsn: 0.869 ± 0.137
0.404CysPro: 0.404 ± 0.077
0.445CysGln: 0.445 ± 0.087
0.445CysArg: 0.445 ± 0.093
1.051CysSer: 1.051 ± 0.147
0.95CysThr: 0.95 ± 0.169
0.525CysVal: 0.525 ± 0.106
0.121CysTrp: 0.121 ± 0.047
0.707CysTyr: 0.707 ± 0.143
0.0CysXaa: 0.0 ± 0.0
Asp
1.859AspAla: 1.859 ± 0.22
0.647AspCys: 0.647 ± 0.123
3.496AspAsp: 3.496 ± 0.304
4.951AspGlu: 4.951 ± 0.413
2.546AspPhe: 2.546 ± 0.268
3.436AspGly: 3.436 ± 0.263
0.667AspHis: 0.667 ± 0.122
7.235AspIle: 7.235 ± 0.382
7.578AspLys: 7.578 ± 0.342
5.355AspLeu: 5.355 ± 0.363
1.617AspMet: 1.617 ± 0.192
5.194AspAsn: 5.194 ± 0.454
0.687AspPro: 0.687 ± 0.131
0.93AspGln: 0.93 ± 0.124
2.203AspArg: 2.203 ± 0.224
3.213AspSer: 3.213 ± 0.292
2.688AspThr: 2.688 ± 0.257
3.233AspVal: 3.233 ± 0.265
0.626AspTrp: 0.626 ± 0.123
3.456AspTyr: 3.456 ± 0.25
0.0AspXaa: 0.0 ± 0.0
Glu
2.385GluAla: 2.385 ± 0.211
0.808GluCys: 0.808 ± 0.134
4.729GluAsp: 4.729 ± 0.292
5.638GluGlu: 5.638 ± 0.416
3.193GluPhe: 3.193 ± 0.242
3.476GluGly: 3.476 ± 0.286
0.808GluHis: 0.808 ± 0.136
7.942GluIle: 7.942 ± 0.394
6.952GluLys: 6.952 ± 0.425
7.801GluLeu: 7.801 ± 0.569
1.718GluMet: 1.718 ± 0.205
6.002GluAsn: 6.002 ± 0.33
0.728GluPro: 0.728 ± 0.135
3.456GluGln: 3.456 ± 0.29
2.385GluArg: 2.385 ± 0.213
3.597GluSer: 3.597 ± 0.208
3.153GluThr: 3.153 ± 0.21
3.678GluVal: 3.678 ± 0.262
0.869GluTrp: 0.869 ± 0.132
4.769GluTyr: 4.769 ± 0.311
0.0GluXaa: 0.0 ± 0.0
Phe
1.354PheAla: 1.354 ± 0.147
0.748PheCys: 0.748 ± 0.145
2.587PheAsp: 2.587 ± 0.252
2.526PheGlu: 2.526 ± 0.252
1.314PhePhe: 1.314 ± 0.192
1.778PheGly: 1.778 ± 0.166
0.505PheHis: 0.505 ± 0.077
3.921PheIle: 3.921 ± 0.342
5.093PheLys: 5.093 ± 0.367
2.849PheLeu: 2.849 ± 0.241
0.869PheMet: 0.869 ± 0.14
4.062PheAsn: 4.062 ± 0.299
1.192PhePro: 1.192 ± 0.21
1.132PheGln: 1.132 ± 0.148
1.172PheArg: 1.172 ± 0.169
2.243PheSer: 2.243 ± 0.241
2.344PheThr: 2.344 ± 0.246
2.041PheVal: 2.041 ± 0.18
0.323PheTrp: 0.323 ± 0.081
1.98PheTyr: 1.98 ± 0.187
0.0PheXaa: 0.0 ± 0.0
Gly
1.415GlyAla: 1.415 ± 0.186
0.728GlyCys: 0.728 ± 0.107
2.829GlyAsp: 2.829 ± 0.258
3.375GlyGlu: 3.375 ± 0.275
2.122GlyPhe: 2.122 ± 0.206
2.627GlyGly: 2.627 ± 0.299
0.606GlyHis: 0.606 ± 0.095
4.83GlyIle: 4.83 ± 0.399
5.072GlyLys: 5.072 ± 0.352
4.426GlyLeu: 4.426 ± 0.359
1.273GlyMet: 1.273 ± 0.182
3.739GlyAsn: 3.739 ± 0.304
0.162GlyPro: 0.162 ± 0.094
1.435GlyGln: 1.435 ± 0.15
1.415GlyArg: 1.415 ± 0.189
2.849GlySer: 2.849 ± 0.267
2.263GlyThr: 2.263 ± 0.257
3.334GlyVal: 3.334 ± 0.302
0.505GlyTrp: 0.505 ± 0.091
2.506GlyTyr: 2.506 ± 0.234
0.0GlyXaa: 0.0 ± 0.0
His
0.586HisAla: 0.586 ± 0.113
0.202HisCys: 0.202 ± 0.069
0.768HisAsp: 0.768 ± 0.117
0.869HisGlu: 0.869 ± 0.128
0.748HisPhe: 0.748 ± 0.126
0.707HisGly: 0.707 ± 0.114
0.364HisHis: 0.364 ± 0.082
1.536HisIle: 1.536 ± 0.184
1.475HisLys: 1.475 ± 0.194
1.111HisLeu: 1.111 ± 0.157
0.283HisMet: 0.283 ± 0.092
1.475HisAsn: 1.475 ± 0.189
0.283HisPro: 0.283 ± 0.098
0.485HisGln: 0.485 ± 0.097
0.505HisArg: 0.505 ± 0.119
0.849HisSer: 0.849 ± 0.127
0.748HisThr: 0.748 ± 0.122
0.505HisVal: 0.505 ± 0.124
0.182HisTrp: 0.182 ± 0.061
0.93HisTyr: 0.93 ± 0.136
0.0HisXaa: 0.0 ± 0.0
Ile
3.658IleAla: 3.658 ± 0.286
1.778IleCys: 1.778 ± 0.22
7.356IleAsp: 7.356 ± 0.392
7.275IleGlu: 7.275 ± 0.439
3.496IlePhe: 3.496 ± 0.303
3.84IleGly: 3.84 ± 0.285
1.314IleHis: 1.314 ± 0.169
8.245IleIle: 8.245 ± 0.481
11.519IleLys: 11.519 ± 0.62
7.114IleLeu: 7.114 ± 0.399
1.96IleMet: 1.96 ± 0.191
9.195IleAsn: 9.195 ± 0.465
2.668IlePro: 2.668 ± 0.282
4.001IleGln: 4.001 ± 0.285
3.173IleArg: 3.173 ± 0.274
6.77IleSer: 6.77 ± 0.478
4.628IleThr: 4.628 ± 0.32
4.385IleVal: 4.385 ± 0.408
0.647IleTrp: 0.647 ± 0.118
4.951IleTyr: 4.951 ± 0.37
0.0IleXaa: 0.0 ± 0.0
Lys
4.486LysAla: 4.486 ± 0.367
1.799LysCys: 1.799 ± 0.258
7.639LysAsp: 7.639 ± 0.415
10.529LysGlu: 10.529 ± 0.582
4.385LysPhe: 4.385 ± 0.369
4.85LysGly: 4.85 ± 0.389
1.879LysHis: 1.879 ± 0.192
9.377LysIle: 9.377 ± 0.464
10.529LysLys: 10.529 ± 0.67
9.357LysLeu: 9.357 ± 0.542
2.445LysMet: 2.445 ± 0.229
8.771LysAsn: 8.771 ± 0.401
2.647LysPro: 2.647 ± 0.242
5.295LysGln: 5.295 ± 0.349
3.274LysArg: 3.274 ± 0.361
6.042LysSer: 6.042 ± 0.383
5.578LysThr: 5.578 ± 0.375
5.699LysVal: 5.699 ± 0.344
1.273LysTrp: 1.273 ± 0.177
6.75LysTyr: 6.75 ± 0.356
0.0LysXaa: 0.0 ± 0.0
Leu
3.112LeuAla: 3.112 ± 0.265
0.97LeuCys: 0.97 ± 0.146
5.376LeuAsp: 5.376 ± 0.316
6.265LeuGlu: 6.265 ± 0.372
3.052LeuPhe: 3.052 ± 0.26
3.779LeuGly: 3.779 ± 0.307
1.213LeuHis: 1.213 ± 0.19
7.295LeuIle: 7.295 ± 0.389
10.367LeuLys: 10.367 ± 0.524
6.568LeuLeu: 6.568 ± 0.439
1.799LeuMet: 1.799 ± 0.195
7.74LeuAsn: 7.74 ± 0.38
1.9LeuPro: 1.9 ± 0.199
3.355LeuGln: 3.355 ± 0.295
2.87LeuArg: 2.87 ± 0.228
5.8LeuSer: 5.8 ± 0.401
4.062LeuThr: 4.062 ± 0.298
3.638LeuVal: 3.638 ± 0.266
0.586LeuTrp: 0.586 ± 0.105
3.678LeuTyr: 3.678 ± 0.322
0.0LeuXaa: 0.0 ± 0.0
Met
1.172MetAla: 1.172 ± 0.172
0.384MetCys: 0.384 ± 0.086
1.394MetAsp: 1.394 ± 0.176
1.617MetGlu: 1.617 ± 0.188
0.728MetPhe: 0.728 ± 0.125
1.334MetGly: 1.334 ± 0.174
0.182MetHis: 0.182 ± 0.064
2.082MetIle: 2.082 ± 0.236
2.526MetLys: 2.526 ± 0.246
2.082MetLeu: 2.082 ± 0.204
0.687MetMet: 0.687 ± 0.113
2.061MetAsn: 2.061 ± 0.21
0.525MetPro: 0.525 ± 0.115
0.707MetGln: 0.707 ± 0.142
0.667MetArg: 0.667 ± 0.111
1.415MetSer: 1.415 ± 0.157
1.213MetThr: 1.213 ± 0.156
1.273MetVal: 1.273 ± 0.136
0.141MetTrp: 0.141 ± 0.056
0.95MetTyr: 0.95 ± 0.143
0.0MetXaa: 0.0 ± 0.0
Asn
2.748AsnAla: 2.748 ± 0.223
1.213AsnCys: 1.213 ± 0.157
4.507AsnAsp: 4.507 ± 0.374
6.406AsnGlu: 6.406 ± 0.436
2.93AsnPhe: 2.93 ± 0.267
4.385AsnGly: 4.385 ± 0.311
1.091AsnHis: 1.091 ± 0.165
8.912AsnIle: 8.912 ± 0.586
11.721AsnLys: 11.721 ± 0.466
6.426AsnLeu: 6.426 ± 0.43
2.284AsnMet: 2.284 ± 0.221
8.387AsnAsn: 8.387 ± 0.53
1.94AsnPro: 1.94 ± 0.203
2.769AsnGln: 2.769 ± 0.231
2.829AsnArg: 2.829 ± 0.265
4.527AsnSer: 4.527 ± 0.389
4.305AsnThr: 4.305 ± 0.401
4.183AsnVal: 4.183 ± 0.268
0.728AsnTrp: 0.728 ± 0.123
3.779AsnTyr: 3.779 ± 0.325
0.0AsnXaa: 0.0 ± 0.0
Pro
0.707ProAla: 0.707 ± 0.107
0.344ProCys: 0.344 ± 0.083
0.829ProAsp: 0.829 ± 0.144
1.213ProGlu: 1.213 ± 0.189
1.01ProPhe: 1.01 ± 0.14
0.182ProGly: 0.182 ± 0.083
0.323ProHis: 0.323 ± 0.086
2.445ProIle: 2.445 ± 0.222
2.506ProLys: 2.506 ± 0.272
1.597ProLeu: 1.597 ± 0.199
0.465ProMet: 0.465 ± 0.099
1.778ProAsn: 1.778 ± 0.241
0.465ProPro: 0.465 ± 0.107
0.525ProGln: 0.525 ± 0.103
0.728ProArg: 0.728 ± 0.133
1.536ProSer: 1.536 ± 0.179
1.435ProThr: 1.435 ± 0.164
1.051ProVal: 1.051 ± 0.165
0.121ProTrp: 0.121 ± 0.051
1.233ProTyr: 1.233 ± 0.169
0.0ProXaa: 0.0 ± 0.0
Gln
1.698GlnAla: 1.698 ± 0.201
0.445GlnCys: 0.445 ± 0.1
1.9GlnAsp: 1.9 ± 0.202
2.668GlnGlu: 2.668 ± 0.302
1.455GlnPhe: 1.455 ± 0.163
1.94GlnGly: 1.94 ± 0.176
0.647GlnHis: 0.647 ± 0.114
3.638GlnIle: 3.638 ± 0.262
3.395GlnLys: 3.395 ± 0.357
3.759GlnLeu: 3.759 ± 0.32
0.788GlnMet: 0.788 ± 0.139
2.506GlnAsn: 2.506 ± 0.274
0.687GlnPro: 0.687 ± 0.111
1.859GlnGln: 1.859 ± 0.278
1.374GlnArg: 1.374 ± 0.155
2.001GlnSer: 2.001 ± 0.236
1.293GlnThr: 1.293 ± 0.169
1.98GlnVal: 1.98 ± 0.205
0.424GlnTrp: 0.424 ± 0.086
2.082GlnTyr: 2.082 ± 0.187
0.0GlnXaa: 0.0 ± 0.0
Arg
0.93ArgAla: 0.93 ± 0.145
0.384ArgCys: 0.384 ± 0.122
2.061ArgAsp: 2.061 ± 0.228
2.91ArgGlu: 2.91 ± 0.248
1.718ArgPhe: 1.718 ± 0.181
1.657ArgGly: 1.657 ± 0.178
0.485ArgHis: 0.485 ± 0.105
3.092ArgIle: 3.092 ± 0.277
3.84ArgLys: 3.84 ± 0.311
2.991ArgLeu: 2.991 ± 0.262
0.768ArgMet: 0.768 ± 0.129
2.647ArgAsn: 2.647 ± 0.265
0.546ArgPro: 0.546 ± 0.095
1.273ArgGln: 1.273 ± 0.15
1.152ArgArg: 1.152 ± 0.197
1.374ArgSer: 1.374 ± 0.164
1.435ArgThr: 1.435 ± 0.163
2.122ArgVal: 2.122 ± 0.242
0.445ArgTrp: 0.445 ± 0.106
1.738ArgTyr: 1.738 ± 0.185
0.0ArgXaa: 0.0 ± 0.0
Ser
2.344SerAla: 2.344 ± 0.265
0.687SerCys: 0.687 ± 0.127
3.052SerAsp: 3.052 ± 0.249
4.224SerGlu: 4.224 ± 0.273
2.526SerPhe: 2.526 ± 0.233
2.89SerGly: 2.89 ± 0.269
0.93SerHis: 0.93 ± 0.167
6.002SerIle: 6.002 ± 0.431
7.033SerLys: 7.033 ± 0.355
5.133SerLeu: 5.133 ± 0.368
1.293SerMet: 1.293 ± 0.193
5.275SerAsn: 5.275 ± 0.407
1.213SerPro: 1.213 ± 0.184
1.94SerGln: 1.94 ± 0.245
2.162SerArg: 2.162 ± 0.216
3.597SerSer: 3.597 ± 0.276
2.951SerThr: 2.951 ± 0.265
2.587SerVal: 2.587 ± 0.26
0.465SerTrp: 0.465 ± 0.088
2.445SerTyr: 2.445 ± 0.281
0.0SerXaa: 0.0 ± 0.0
Thr
1.597ThrAla: 1.597 ± 0.18
0.465ThrCys: 0.465 ± 0.095
2.87ThrAsp: 2.87 ± 0.259
3.355ThrGlu: 3.355 ± 0.233
2.203ThrPhe: 2.203 ± 0.24
2.364ThrGly: 2.364 ± 0.255
1.051ThrHis: 1.051 ± 0.143
5.153ThrIle: 5.153 ± 0.304
4.81ThrLys: 4.81 ± 0.346
3.941ThrLeu: 3.941 ± 0.292
1.091ThrMet: 1.091 ± 0.183
3.9ThrAsn: 3.9 ± 0.318
1.415ThrPro: 1.415 ± 0.176
1.617ThrGln: 1.617 ± 0.186
2.082ThrArg: 2.082 ± 0.174
3.254ThrSer: 3.254 ± 0.364
2.89ThrThr: 2.89 ± 0.279
2.203ThrVal: 2.203 ± 0.225
0.505ThrTrp: 0.505 ± 0.107
2.93ThrTyr: 2.93 ± 0.253
0.0ThrXaa: 0.0 ± 0.0
Val
2.344ValAla: 2.344 ± 0.244
0.909ValCys: 0.909 ± 0.156
3.597ValAsp: 3.597 ± 0.293
3.092ValGlu: 3.092 ± 0.211
1.799ValPhe: 1.799 ± 0.212
2.89ValGly: 2.89 ± 0.26
0.788ValHis: 0.788 ± 0.126
4.183ValIle: 4.183 ± 0.303
5.517ValLys: 5.517 ± 0.347
4.001ValLeu: 4.001 ± 0.307
0.99ValMet: 0.99 ± 0.142
4.062ValAsn: 4.062 ± 0.273
1.091ValPro: 1.091 ± 0.146
1.718ValGln: 1.718 ± 0.158
1.637ValArg: 1.637 ± 0.198
3.213ValSer: 3.213 ± 0.271
2.385ValThr: 2.385 ± 0.278
3.112ValVal: 3.112 ± 0.206
0.465ValTrp: 0.465 ± 0.107
2.142ValTyr: 2.142 ± 0.22
0.0ValXaa: 0.0 ± 0.0
Trp
0.283TrpAla: 0.283 ± 0.072
0.243TrpCys: 0.243 ± 0.075
0.647TrpAsp: 0.647 ± 0.129
0.525TrpGlu: 0.525 ± 0.106
0.384TrpPhe: 0.384 ± 0.089
0.566TrpGly: 0.566 ± 0.124
0.061TrpHis: 0.061 ± 0.034
0.97TrpIle: 0.97 ± 0.138
0.667TrpLys: 0.667 ± 0.121
0.808TrpLeu: 0.808 ± 0.115
0.283TrpMet: 0.283 ± 0.079
1.172TrpAsn: 1.172 ± 0.14
0.0TrpPro: 0.0 ± 0.0
0.424TrpGln: 0.424 ± 0.108
0.243TrpArg: 0.243 ± 0.063
0.384TrpSer: 0.384 ± 0.094
0.505TrpThr: 0.505 ± 0.082
0.445TrpVal: 0.445 ± 0.11
0.081TrpTrp: 0.081 ± 0.039
0.525TrpTyr: 0.525 ± 0.097
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.758TyrAla: 1.758 ± 0.224
0.889TyrCys: 0.889 ± 0.136
3.395TyrAsp: 3.395 ± 0.275
3.718TyrGlu: 3.718 ± 0.293
2.364TyrPhe: 2.364 ± 0.275
2.001TyrGly: 2.001 ± 0.22
0.707TyrHis: 0.707 ± 0.12
5.638TyrIle: 5.638 ± 0.341
5.881TyrLys: 5.881 ± 0.326
3.88TyrLeu: 3.88 ± 0.281
1.273TyrMet: 1.273 ± 0.164
4.769TyrAsn: 4.769 ± 0.294
1.192TyrPro: 1.192 ± 0.188
1.536TyrGln: 1.536 ± 0.186
1.94TyrArg: 1.94 ± 0.227
2.89TyrSer: 2.89 ± 0.253
2.647TyrThr: 2.647 ± 0.245
2.486TyrVal: 2.486 ± 0.231
0.323TyrTrp: 0.323 ± 0.085
2.364TyrTyr: 2.364 ± 0.249
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 192 proteins (49484 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski