Amino acid dipepetide frequency for Gordonia phage Affeca

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.184AlaAla: 16.184 ± 1.125
0.846AlaCys: 0.846 ± 0.244
8.303AlaAsp: 8.303 ± 0.712
9.678AlaGlu: 9.678 ± 0.846
2.962AlaPhe: 2.962 ± 0.508
11.159AlaGly: 11.159 ± 0.931
1.745AlaHis: 1.745 ± 0.291
6.241AlaIle: 6.241 ± 0.881
3.385AlaLys: 3.385 ± 0.477
8.938AlaLeu: 8.938 ± 1.087
2.539AlaMet: 2.539 ± 0.441
3.067AlaAsn: 3.067 ± 0.472
6.558AlaPro: 6.558 ± 0.64
4.178AlaGln: 4.178 ± 0.393
8.621AlaArg: 8.621 ± 0.764
5.765AlaSer: 5.765 ± 0.661
7.669AlaThr: 7.669 ± 0.789
8.039AlaVal: 8.039 ± 0.694
2.539AlaTrp: 2.539 ± 0.315
2.116AlaTyr: 2.116 ± 0.341
0.0AlaXaa: 0.0 ± 0.0
Cys
1.322CysAla: 1.322 ± 0.332
0.053CysCys: 0.053 ± 0.051
0.635CysAsp: 0.635 ± 0.219
0.529CysGlu: 0.529 ± 0.179
0.159CysPhe: 0.159 ± 0.077
1.481CysGly: 1.481 ± 0.323
0.635CysHis: 0.635 ± 0.192
0.053CysIle: 0.053 ± 0.056
0.159CysLys: 0.159 ± 0.089
0.159CysLeu: 0.159 ± 0.097
0.159CysMet: 0.159 ± 0.091
0.159CysAsn: 0.159 ± 0.093
0.952CysPro: 0.952 ± 0.2
0.106CysGln: 0.106 ± 0.08
0.582CysArg: 0.582 ± 0.207
0.476CysSer: 0.476 ± 0.148
0.635CysThr: 0.635 ± 0.188
0.635CysVal: 0.635 ± 0.224
0.106CysTrp: 0.106 ± 0.074
0.106CysTyr: 0.106 ± 0.08
0.0CysXaa: 0.0 ± 0.0
Asp
8.145AspAla: 8.145 ± 0.649
0.37AspCys: 0.37 ± 0.157
7.51AspAsp: 7.51 ± 0.924
5.923AspGlu: 5.923 ± 0.684
1.216AspPhe: 1.216 ± 0.302
7.51AspGly: 7.51 ± 0.747
1.851AspHis: 1.851 ± 0.392
1.798AspIle: 1.798 ± 0.347
1.587AspLys: 1.587 ± 0.261
4.654AspLeu: 4.654 ± 0.485
1.692AspMet: 1.692 ± 0.268
2.116AspAsn: 2.116 ± 0.346
7.14AspPro: 7.14 ± 0.899
2.591AspGln: 2.591 ± 0.464
5.712AspArg: 5.712 ± 0.559
2.274AspSer: 2.274 ± 0.318
3.755AspThr: 3.755 ± 0.424
6.294AspVal: 6.294 ± 0.687
1.64AspTrp: 1.64 ± 0.332
1.534AspTyr: 1.534 ± 0.316
0.0AspXaa: 0.0 ± 0.0
Glu
5.395GluAla: 5.395 ± 0.565
0.74GluCys: 0.74 ± 0.235
3.385GluAsp: 3.385 ± 0.494
1.375GluGlu: 1.375 ± 0.284
2.01GluPhe: 2.01 ± 0.329
3.808GluGly: 3.808 ± 0.539
2.486GluHis: 2.486 ± 0.399
2.221GluIle: 2.221 ± 0.271
1.216GluLys: 1.216 ± 0.346
4.971GluLeu: 4.971 ± 0.773
1.058GluMet: 1.058 ± 0.242
1.534GluAsn: 1.534 ± 0.302
3.385GluPro: 3.385 ± 0.509
3.861GluGln: 3.861 ± 0.474
4.654GluArg: 4.654 ± 0.529
2.803GluSer: 2.803 ± 0.501
3.173GluThr: 3.173 ± 0.448
5.236GluVal: 5.236 ± 0.645
1.692GluTrp: 1.692 ± 0.315
1.798GluTyr: 1.798 ± 0.299
0.0GluXaa: 0.0 ± 0.0
Phe
3.067PheAla: 3.067 ± 0.395
0.264PheCys: 0.264 ± 0.129
2.644PheAsp: 2.644 ± 0.375
1.745PheGlu: 1.745 ± 0.391
0.635PhePhe: 0.635 ± 0.22
2.75PheGly: 2.75 ± 0.367
0.635PheHis: 0.635 ± 0.147
1.269PheIle: 1.269 ± 0.317
0.688PheLys: 0.688 ± 0.15
1.798PheLeu: 1.798 ± 0.354
0.476PheMet: 0.476 ± 0.129
0.846PheAsn: 0.846 ± 0.222
1.164PhePro: 1.164 ± 0.259
0.582PheGln: 0.582 ± 0.199
1.058PheArg: 1.058 ± 0.238
1.111PheSer: 1.111 ± 0.239
1.904PheThr: 1.904 ± 0.304
2.433PheVal: 2.433 ± 0.252
0.264PheTrp: 0.264 ± 0.12
0.212PheTyr: 0.212 ± 0.099
0.0PheXaa: 0.0 ± 0.0
Gly
8.779GlyAla: 8.779 ± 1.017
0.846GlyCys: 0.846 ± 0.225
6.505GlyAsp: 6.505 ± 0.764
5.024GlyGlu: 5.024 ± 0.54
1.904GlyPhe: 1.904 ± 0.369
7.457GlyGly: 7.457 ± 0.941
2.327GlyHis: 2.327 ± 0.382
4.39GlyIle: 4.39 ± 0.467
2.962GlyLys: 2.962 ± 0.438
5.871GlyLeu: 5.871 ± 0.697
1.692GlyMet: 1.692 ± 0.265
2.75GlyAsn: 2.75 ± 0.478
3.914GlyPro: 3.914 ± 0.484
3.279GlyGln: 3.279 ± 0.37
6.611GlyArg: 6.611 ± 0.542
4.971GlySer: 4.971 ± 0.509
5.395GlyThr: 5.395 ± 0.704
6.981GlyVal: 6.981 ± 0.587
1.375GlyTrp: 1.375 ± 0.273
2.01GlyTyr: 2.01 ± 0.357
0.0GlyXaa: 0.0 ± 0.0
His
2.38HisAla: 2.38 ± 0.451
0.212HisCys: 0.212 ± 0.106
2.433HisAsp: 2.433 ± 0.433
1.64HisGlu: 1.64 ± 0.287
0.529HisPhe: 0.529 ± 0.161
1.428HisGly: 1.428 ± 0.33
0.476HisHis: 0.476 ± 0.218
0.846HisIle: 0.846 ± 0.236
0.317HisLys: 0.317 ± 0.12
1.534HisLeu: 1.534 ± 0.275
0.582HisMet: 0.582 ± 0.179
0.476HisAsn: 0.476 ± 0.174
1.587HisPro: 1.587 ± 0.327
1.111HisGln: 1.111 ± 0.205
1.851HisArg: 1.851 ± 0.316
0.899HisSer: 0.899 ± 0.184
1.957HisThr: 1.957 ± 0.377
1.745HisVal: 1.745 ± 0.283
0.37HisTrp: 0.37 ± 0.124
0.529HisTyr: 0.529 ± 0.175
0.0HisXaa: 0.0 ± 0.0
Ile
6.241IleAla: 6.241 ± 0.537
0.212IleCys: 0.212 ± 0.117
4.019IleAsp: 4.019 ± 0.526
3.543IleGlu: 3.543 ± 0.412
0.423IlePhe: 0.423 ± 0.208
5.183IleGly: 5.183 ± 0.898
0.529IleHis: 0.529 ± 0.168
2.591IleIle: 2.591 ± 0.45
1.058IleLys: 1.058 ± 0.296
2.063IleLeu: 2.063 ± 0.312
0.74IleMet: 0.74 ± 0.232
0.846IleAsn: 0.846 ± 0.214
3.12IlePro: 3.12 ± 0.285
1.322IleGln: 1.322 ± 0.281
2.962IleArg: 2.962 ± 0.446
1.587IleSer: 1.587 ± 0.342
3.702IleThr: 3.702 ± 0.474
3.755IleVal: 3.755 ± 0.436
0.635IleTrp: 0.635 ± 0.173
0.952IleTyr: 0.952 ± 0.232
0.0IleXaa: 0.0 ± 0.0
Lys
2.856LysAla: 2.856 ± 0.38
0.159LysCys: 0.159 ± 0.088
0.899LysAsp: 0.899 ± 0.214
0.582LysGlu: 0.582 ± 0.14
0.952LysPhe: 0.952 ± 0.282
1.375LysGly: 1.375 ± 0.285
0.793LysHis: 0.793 ± 0.229
1.269LysIle: 1.269 ± 0.27
0.37LysLys: 0.37 ± 0.154
2.221LysLeu: 2.221 ± 0.406
0.582LysMet: 0.582 ± 0.167
0.846LysAsn: 0.846 ± 0.235
1.745LysPro: 1.745 ± 0.381
1.058LysGln: 1.058 ± 0.228
2.221LysArg: 2.221 ± 0.358
1.745LysSer: 1.745 ± 0.354
1.745LysThr: 1.745 ± 0.289
2.539LysVal: 2.539 ± 0.386
0.37LysTrp: 0.37 ± 0.133
0.846LysTyr: 0.846 ± 0.231
0.0LysXaa: 0.0 ± 0.0
Leu
8.938LeuAla: 8.938 ± 0.816
0.74LeuCys: 0.74 ± 0.236
5.606LeuAsp: 5.606 ± 0.54
2.274LeuGlu: 2.274 ± 0.287
2.168LeuPhe: 2.168 ± 0.35
6.717LeuGly: 6.717 ± 0.858
1.587LeuHis: 1.587 ± 0.311
3.067LeuIle: 3.067 ± 0.307
1.058LeuLys: 1.058 ± 0.221
5.712LeuLeu: 5.712 ± 0.549
1.269LeuMet: 1.269 ± 0.234
2.168LeuAsn: 2.168 ± 0.361
4.443LeuPro: 4.443 ± 0.445
3.12LeuGln: 3.12 ± 0.415
5.923LeuArg: 5.923 ± 0.468
3.596LeuSer: 3.596 ± 0.419
6.188LeuThr: 6.188 ± 0.554
5.024LeuVal: 5.024 ± 0.485
1.904LeuTrp: 1.904 ± 0.329
1.269LeuTyr: 1.269 ± 0.284
0.0LeuXaa: 0.0 ± 0.0
Met
2.221MetAla: 2.221 ± 0.264
0.159MetCys: 0.159 ± 0.092
0.899MetAsp: 0.899 ± 0.196
0.74MetGlu: 0.74 ± 0.222
0.74MetPhe: 0.74 ± 0.23
0.952MetGly: 0.952 ± 0.256
0.317MetHis: 0.317 ± 0.12
0.846MetIle: 0.846 ± 0.18
0.899MetLys: 0.899 ± 0.227
1.957MetLeu: 1.957 ± 0.331
0.212MetMet: 0.212 ± 0.102
0.529MetAsn: 0.529 ± 0.184
1.798MetPro: 1.798 ± 0.226
0.846MetGln: 0.846 ± 0.276
1.534MetArg: 1.534 ± 0.277
1.481MetSer: 1.481 ± 0.235
3.491MetThr: 3.491 ± 0.402
1.428MetVal: 1.428 ± 0.297
0.423MetTrp: 0.423 ± 0.154
0.106MetTyr: 0.106 ± 0.07
0.0MetXaa: 0.0 ± 0.0
Asn
3.543AsnAla: 3.543 ± 0.526
0.423AsnCys: 0.423 ± 0.152
1.904AsnAsp: 1.904 ± 0.303
1.587AsnGlu: 1.587 ± 0.312
0.688AsnPhe: 0.688 ± 0.169
2.75AsnGly: 2.75 ± 0.363
0.635AsnHis: 0.635 ± 0.139
0.74AsnIle: 0.74 ± 0.196
0.317AsnLys: 0.317 ± 0.149
2.01AsnLeu: 2.01 ± 0.397
0.688AsnMet: 0.688 ± 0.18
0.529AsnAsn: 0.529 ± 0.15
2.327AsnPro: 2.327 ± 0.406
0.74AsnGln: 0.74 ± 0.244
1.957AsnArg: 1.957 ± 0.396
1.058AsnSer: 1.058 ± 0.238
1.534AsnThr: 1.534 ± 0.284
1.587AsnVal: 1.587 ± 0.27
0.793AsnTrp: 0.793 ± 0.229
0.635AsnTyr: 0.635 ± 0.155
0.0AsnXaa: 0.0 ± 0.0
Pro
8.409ProAla: 8.409 ± 0.79
0.529ProCys: 0.529 ± 0.246
5.976ProAsp: 5.976 ± 0.756
3.543ProGlu: 3.543 ± 0.56
1.745ProPhe: 1.745 ± 0.335
4.813ProGly: 4.813 ± 0.464
1.058ProHis: 1.058 ± 0.23
3.438ProIle: 3.438 ± 0.377
1.745ProLys: 1.745 ± 0.374
4.019ProLeu: 4.019 ± 0.475
1.481ProMet: 1.481 ± 0.249
2.063ProAsn: 2.063 ± 0.396
4.443ProPro: 4.443 ± 0.576
2.168ProGln: 2.168 ± 0.363
3.332ProArg: 3.332 ± 0.376
3.332ProSer: 3.332 ± 0.425
4.019ProThr: 4.019 ± 0.486
4.866ProVal: 4.866 ± 0.57
1.111ProTrp: 1.111 ± 0.266
1.322ProTyr: 1.322 ± 0.269
0.0ProXaa: 0.0 ± 0.0
Gln
5.13GlnAla: 5.13 ± 0.451
0.423GlnCys: 0.423 ± 0.143
1.164GlnAsp: 1.164 ± 0.324
0.793GlnGlu: 0.793 ± 0.18
1.322GlnPhe: 1.322 ± 0.207
1.957GlnGly: 1.957 ± 0.325
0.952GlnHis: 0.952 ± 0.207
1.957GlnIle: 1.957 ± 0.358
0.899GlnLys: 0.899 ± 0.202
3.332GlnLeu: 3.332 ± 0.312
1.058GlnMet: 1.058 ± 0.244
0.635GlnAsn: 0.635 ± 0.177
2.803GlnPro: 2.803 ± 0.393
2.644GlnGln: 2.644 ± 0.367
3.543GlnArg: 3.543 ± 0.487
1.534GlnSer: 1.534 ± 0.294
2.168GlnThr: 2.168 ± 0.327
2.909GlnVal: 2.909 ± 0.371
0.899GlnTrp: 0.899 ± 0.198
0.952GlnTyr: 0.952 ± 0.176
0.0GlnXaa: 0.0 ± 0.0
Arg
9.255ArgAla: 9.255 ± 0.771
0.582ArgCys: 0.582 ± 0.168
4.919ArgAsp: 4.919 ± 0.445
3.649ArgGlu: 3.649 ± 0.506
1.904ArgPhe: 1.904 ± 0.359
4.495ArgGly: 4.495 ± 0.67
2.063ArgHis: 2.063 ± 0.397
3.914ArgIle: 3.914 ± 0.518
2.327ArgLys: 2.327 ± 0.414
5.5ArgLeu: 5.5 ± 0.506
2.168ArgMet: 2.168 ± 0.249
2.01ArgAsn: 2.01 ± 0.32
3.755ArgPro: 3.755 ± 0.446
2.433ArgGln: 2.433 ± 0.319
8.356ArgArg: 8.356 ± 1.057
3.015ArgSer: 3.015 ± 0.383
4.495ArgThr: 4.495 ± 0.473
5.976ArgVal: 5.976 ± 0.786
1.798ArgTrp: 1.798 ± 0.311
2.063ArgTyr: 2.063 ± 0.352
0.0ArgXaa: 0.0 ± 0.0
Ser
5.236SerAla: 5.236 ± 0.648
0.423SerCys: 0.423 ± 0.156
3.649SerAsp: 3.649 ± 0.485
2.591SerGlu: 2.591 ± 0.419
1.164SerPhe: 1.164 ± 0.26
5.236SerGly: 5.236 ± 0.737
0.952SerHis: 0.952 ± 0.178
2.063SerIle: 2.063 ± 0.403
1.322SerLys: 1.322 ± 0.257
3.015SerLeu: 3.015 ± 0.394
0.952SerMet: 0.952 ± 0.224
0.899SerAsn: 0.899 ± 0.23
3.12SerPro: 3.12 ± 0.43
1.164SerGln: 1.164 ± 0.273
3.332SerArg: 3.332 ± 0.482
2.856SerSer: 2.856 ± 0.493
4.072SerThr: 4.072 ± 0.537
4.072SerVal: 4.072 ± 0.386
1.005SerTrp: 1.005 ± 0.246
0.846SerTyr: 0.846 ± 0.2
0.0SerXaa: 0.0 ± 0.0
Thr
9.837ThrAla: 9.837 ± 0.802
1.005ThrCys: 1.005 ± 0.288
4.813ThrAsp: 4.813 ± 0.561
3.543ThrGlu: 3.543 ± 0.377
1.428ThrPhe: 1.428 ± 0.28
6.188ThrGly: 6.188 ± 0.729
1.481ThrHis: 1.481 ± 0.339
3.438ThrIle: 3.438 ± 0.458
1.904ThrLys: 1.904 ± 0.298
5.077ThrLeu: 5.077 ± 0.449
1.692ThrMet: 1.692 ± 0.277
1.692ThrAsn: 1.692 ± 0.286
5.183ThrPro: 5.183 ± 0.424
1.587ThrGln: 1.587 ± 0.246
4.019ThrArg: 4.019 ± 0.445
3.543ThrSer: 3.543 ± 0.439
4.919ThrThr: 4.919 ± 0.712
4.919ThrVal: 4.919 ± 0.526
1.322ThrTrp: 1.322 ± 0.253
1.428ThrTyr: 1.428 ± 0.317
0.0ThrXaa: 0.0 ± 0.0
Val
8.515ValAla: 8.515 ± 0.884
0.74ValCys: 0.74 ± 0.273
7.14ValAsp: 7.14 ± 0.609
5.289ValGlu: 5.289 ± 0.583
2.221ValPhe: 2.221 ± 0.308
6.558ValGly: 6.558 ± 0.547
1.745ValHis: 1.745 ± 0.316
3.702ValIle: 3.702 ± 0.402
1.692ValLys: 1.692 ± 0.243
6.188ValLeu: 6.188 ± 0.541
1.164ValMet: 1.164 ± 0.185
2.591ValAsn: 2.591 ± 0.39
3.755ValPro: 3.755 ± 0.403
2.697ValGln: 2.697 ± 0.288
4.707ValArg: 4.707 ± 0.571
3.702ValSer: 3.702 ± 0.457
5.712ValThr: 5.712 ± 0.51
6.452ValVal: 6.452 ± 0.628
1.851ValTrp: 1.851 ± 0.214
2.116ValTyr: 2.116 ± 0.358
0.0ValXaa: 0.0 ± 0.0
Trp
2.327TrpAla: 2.327 ± 0.371
0.212TrpCys: 0.212 ± 0.09
1.005TrpAsp: 1.005 ± 0.183
0.952TrpGlu: 0.952 ± 0.208
0.74TrpPhe: 0.74 ± 0.18
1.216TrpGly: 1.216 ± 0.237
0.264TrpHis: 0.264 ± 0.117
0.952TrpIle: 0.952 ± 0.283
0.793TrpLys: 0.793 ± 0.206
2.168TrpLeu: 2.168 ± 0.313
0.846TrpMet: 0.846 ± 0.221
0.423TrpAsn: 0.423 ± 0.169
1.164TrpPro: 1.164 ± 0.202
1.111TrpGln: 1.111 ± 0.209
1.745TrpArg: 1.745 ± 0.374
1.428TrpSer: 1.428 ± 0.248
1.111TrpThr: 1.111 ± 0.287
1.745TrpVal: 1.745 ± 0.275
0.37TrpTrp: 0.37 ± 0.159
0.423TrpTyr: 0.423 ± 0.127
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.75TyrAla: 2.75 ± 0.45
0.159TyrCys: 0.159 ± 0.088
1.904TyrAsp: 1.904 ± 0.332
1.798TyrGlu: 1.798 ± 0.293
0.635TyrPhe: 0.635 ± 0.142
2.01TyrGly: 2.01 ± 0.286
0.37TyrHis: 0.37 ± 0.127
0.688TyrIle: 0.688 ± 0.174
0.476TyrLys: 0.476 ± 0.157
1.587TyrLeu: 1.587 ± 0.28
0.476TyrMet: 0.476 ± 0.146
0.264TyrAsn: 0.264 ± 0.122
1.058TyrPro: 1.058 ± 0.29
0.529TyrGln: 0.529 ± 0.157
1.957TyrArg: 1.957 ± 0.309
0.793TyrSer: 0.793 ± 0.157
1.375TyrThr: 1.375 ± 0.297
1.904TyrVal: 1.904 ± 0.351
0.529TyrTrp: 0.529 ± 0.182
0.37TyrTyr: 0.37 ± 0.138
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 87 proteins (18909 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski