Alcanivorax sp. N3-2A

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Alcanivoracaceae; Alcanivorax; unclassified Alcanivorax

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3757 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A220SME0|A0A220SME0_9GAMM DctA-YdbH domain-containing protein OS=Alcanivorax sp. N3-2A OX=2014542 GN=CEK62_09465 PE=4 SV=1
MM1 pKa = 7.63LGLAPLAGCDD11 pKa = 4.22NDD13 pKa = 5.21TSDD16 pKa = 4.98HH17 pKa = 7.39DD18 pKa = 4.26NDD20 pKa = 3.8ASNGGGDD27 pKa = 3.36NGGNDD32 pKa = 3.45GGGNDD37 pKa = 5.08GGGNDD42 pKa = 5.54DD43 pKa = 4.98GGSDD47 pKa = 4.63DD48 pKa = 5.41GGDD51 pKa = 3.51NGGDD55 pKa = 3.44NDD57 pKa = 4.35NPPTPTLVITSDD69 pKa = 3.01NATAVLIQSRR79 pKa = 11.84MALDD83 pKa = 3.96LLPRR87 pKa = 11.84LLVGANVFYY96 pKa = 10.84SNSIAVQGYY105 pKa = 10.78DD106 pKa = 5.23DD107 pKa = 6.66FPPCDD112 pKa = 3.45NAEE115 pKa = 4.13GQVQLLGDD123 pKa = 3.84NPGQPTTLIASDD135 pKa = 4.95CDD137 pKa = 3.65DD138 pKa = 3.83LSYY141 pKa = 11.03PRR143 pKa = 11.84NFNGRR148 pKa = 11.84LDD150 pKa = 4.63LDD152 pKa = 4.26FSNADD157 pKa = 3.83DD158 pKa = 4.52PLSTFTGFAEE168 pKa = 5.48RR169 pKa = 11.84IQAYY173 pKa = 9.98DD174 pKa = 3.33PNLRR178 pKa = 11.84LSANGGLRR186 pKa = 11.84LHH188 pKa = 7.09PEE190 pKa = 4.19DD191 pKa = 4.86PDD193 pKa = 4.26DD194 pKa = 4.12YY195 pKa = 11.4FGIAVAHH202 pKa = 5.93QADD205 pKa = 3.37IDD207 pKa = 3.82ITLEE211 pKa = 4.0LLPGGSDD218 pKa = 3.53TPITSLGFDD227 pKa = 3.25FHH229 pKa = 7.75DD230 pKa = 3.71YY231 pKa = 9.16TSRR234 pKa = 11.84YY235 pKa = 8.97SSSIHH240 pKa = 6.34FDD242 pKa = 3.4DD243 pKa = 4.93VPFPLLSGAGDD254 pKa = 3.38VTFRR258 pKa = 11.84GLAEE262 pKa = 4.08GRR264 pKa = 11.84VTLTTPRR271 pKa = 11.84QLTDD275 pKa = 3.72PDD277 pKa = 3.77ASNPLCYY284 pKa = 10.07GDD286 pKa = 4.67GVIEE290 pKa = 4.12VEE292 pKa = 4.23GAEE295 pKa = 4.33GSSVRR300 pKa = 11.84LDD302 pKa = 3.54YY303 pKa = 11.59NGAQAILSVNGALLASGDD321 pKa = 4.27CTDD324 pKa = 5.41LTQQWTSQVAASLPPYY340 pKa = 10.13RR341 pKa = 5.66

Molecular weight: 35.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A220SR68|A0A220SR68_9GAMM CoA transferase OS=Alcanivorax sp. N3-2A OX=2014542 GN=CEK62_16935 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.16RR12 pKa = 11.84KK13 pKa = 9.08RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.2SGRR28 pKa = 11.84KK29 pKa = 8.76ILAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3757

0

3757

1225219

38

4056

326.1

35.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.293 ± 0.058

0.911 ± 0.01

5.932 ± 0.037

5.807 ± 0.035

3.535 ± 0.028

8.49 ± 0.044

2.343 ± 0.024

4.416 ± 0.034

2.936 ± 0.034

11.298 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.402 ± 0.022

2.843 ± 0.028

5.001 ± 0.033

4.052 ± 0.029

7.354 ± 0.049

5.231 ± 0.028

4.878 ± 0.033

7.343 ± 0.037

1.46 ± 0.017

2.475 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski