Pseudomonas phage YMC/01/01/P52_PAE_BP

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J7I4J0|J7I4J0_9CAUD Uncharacterized protein OS=Pseudomonas phage YMC/01/01/P52_PAE_BP OX=1191380 GN=BPPAE11_00150 PE=4 SV=1
MM1 pKa = 7.21STTANWSYY9 pKa = 11.34TNTATVRR16 pKa = 11.84PFLYY20 pKa = 10.35FDD22 pKa = 5.12LSTQEE27 pKa = 4.28AVYY30 pKa = 10.49GPEE33 pKa = 3.9YY34 pKa = 10.45EE35 pKa = 4.86IACTWVAKK43 pKa = 10.53GEE45 pKa = 4.09QVRR48 pKa = 11.84DD49 pKa = 3.47NNGAEE54 pKa = 4.23FVSRR58 pKa = 11.84HH59 pKa = 5.03QIYY62 pKa = 8.8TEE64 pKa = 3.78DD65 pKa = 3.81RR66 pKa = 11.84RR67 pKa = 11.84PKK69 pKa = 10.53YY70 pKa = 10.52LDD72 pKa = 5.18LIQFDD77 pKa = 4.8GSSGWEE83 pKa = 4.14EE84 pKa = 3.71IRR86 pKa = 11.84SVTNWDD92 pKa = 3.02MSFFGEE98 pKa = 5.13DD99 pKa = 3.49PDD101 pKa = 4.53FLLVTT106 pKa = 4.73

Molecular weight: 12.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J7HXJ0|J7HXJ0_9CAUD Uncharacterized protein OS=Pseudomonas phage YMC/01/01/P52_PAE_BP OX=1191380 GN=BPPAE11_00440 PE=4 SV=1
MM1 pKa = 7.26NRR3 pKa = 11.84RR4 pKa = 11.84IAKK7 pKa = 9.87AVGEE11 pKa = 4.25GVLLGLGLAVFMIGLVTTARR31 pKa = 11.84VIFQFVGALAGCQPP45 pKa = 3.97

Molecular weight: 4.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

14646

38

876

203.4

22.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.211 ± 0.618

1.065 ± 0.127

6.131 ± 0.213

6.391 ± 0.341

2.799 ± 0.239

7.975 ± 0.255

2.035 ± 0.204

5.087 ± 0.207

4.076 ± 0.202

8.61 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.567 ± 0.164

3.441 ± 0.26

4.698 ± 0.263

4.342 ± 0.316

7.074 ± 0.336

5.681 ± 0.326

5.592 ± 0.332

6.889 ± 0.323

1.591 ± 0.122

2.745 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski