Dendrothele bispora (strain CBS 962.96)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Agaricales incertae sedis; Dendrothele; Dendrothele bispora

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 33018 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S8LN97|A0A4S8LN97_DENBC HAD-like protein OS=Dendrothele bispora (strain CBS 962.96) OX=1314807 GN=K435DRAFT_759899 PE=4 SV=1
MM1 pKa = 7.66SSEE4 pKa = 4.16SSNLSSLTRR13 pKa = 11.84SKK15 pKa = 10.6LHH17 pKa = 6.54HH18 pKa = 6.44SVGSKK23 pKa = 10.22DD24 pKa = 3.13KK25 pKa = 10.95CSLHH29 pKa = 6.45RR30 pKa = 11.84WVLLKK35 pKa = 11.01NSFVRR40 pKa = 11.84ALPLTTNNTSPSNEE54 pKa = 3.79AMDD57 pKa = 4.59ANYY60 pKa = 10.63SEE62 pKa = 4.64SPRR65 pKa = 11.84GAEE68 pKa = 3.93EE69 pKa = 4.0TASDD73 pKa = 4.19DD74 pKa = 3.63ADD76 pKa = 3.39SFMFPDD82 pKa = 4.05VSNMAEE88 pKa = 3.94SSGVDD93 pKa = 3.32GGASEE98 pKa = 4.91AQWLDD103 pKa = 3.35SLLEE107 pKa = 4.05TLGDD111 pKa = 4.5DD112 pKa = 5.05DD113 pKa = 7.09DD114 pKa = 7.47DD115 pKa = 5.48DD116 pKa = 5.51FSVDD120 pKa = 3.48NVSVLQPDD128 pKa = 4.25DD129 pKa = 5.95DD130 pKa = 5.39DD131 pKa = 5.48DD132 pKa = 6.27HH133 pKa = 6.44MLSPIVSPMSSSDD146 pKa = 3.71DD147 pKa = 3.61LSSVDD152 pKa = 4.97FSSQADD158 pKa = 3.89FSSPIAVSYY167 pKa = 8.15PYY169 pKa = 9.6PVPYY173 pKa = 10.25PPFHH177 pKa = 7.19PPLISYY183 pKa = 8.34HH184 pKa = 5.7QLDD187 pKa = 3.93SSFDD191 pKa = 3.85PLDD194 pKa = 3.31ITGDD198 pKa = 3.7PLPYY202 pKa = 10.07YY203 pKa = 10.45DD204 pKa = 5.86LEE206 pKa = 4.56DD207 pKa = 4.43VEE209 pKa = 5.43DD210 pKa = 4.86LPVPDD215 pKa = 5.8AIEE218 pKa = 4.42DD219 pKa = 3.94TSDD222 pKa = 4.15DD223 pKa = 3.93EE224 pKa = 6.02SDD226 pKa = 3.56TLLTPSVGHH235 pKa = 6.29SSSSLLSLDD244 pKa = 3.91SSPAPLSADD253 pKa = 3.44RR254 pKa = 11.84SRR256 pKa = 11.84SHH258 pKa = 6.68HH259 pKa = 6.6SSPHH263 pKa = 6.61VYY265 pKa = 9.53IDD267 pKa = 3.72SDD269 pKa = 3.72DD270 pKa = 3.93SSFYY274 pKa = 10.46PYY276 pKa = 9.63EE277 pKa = 4.29TDD279 pKa = 3.67PDD281 pKa = 4.13PLPFSDD287 pKa = 5.13HH288 pKa = 6.43LRR290 pKa = 11.84SPYY293 pKa = 10.9NFYY296 pKa = 10.65QEE298 pKa = 4.38CC299 pKa = 3.57

Molecular weight: 32.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S8L737|A0A4S8L737_DENBC Uncharacterized protein (Fragment) OS=Dendrothele bispora (strain CBS 962.96) OX=1314807 GN=K435DRAFT_593729 PE=4 SV=1
MM1 pKa = 8.2RR2 pKa = 11.84IRR4 pKa = 11.84NQSMTSWPRR13 pKa = 11.84WSPRR17 pKa = 11.84RR18 pKa = 11.84PQPPSWSPRR27 pKa = 11.84RR28 pKa = 11.84TPPSPFSKK36 pKa = 10.03PQPQSLTSSSLLPLTSPSTPSSQSPRR62 pKa = 11.84TTPPSQTPSAWPTWPARR79 pKa = 11.84SPSAWQTPLKK89 pKa = 10.01PLSTRR94 pKa = 11.84SLTHH98 pKa = 5.88PTSSS102 pKa = 3.17

Molecular weight: 11.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

33014

4

33018

11777400

49

5921

356.7

39.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.135 ± 0.013

1.356 ± 0.006

5.656 ± 0.016

6.036 ± 0.017

3.993 ± 0.009

6.401 ± 0.016

2.54 ± 0.008

5.147 ± 0.01

4.87 ± 0.013

9.123 ± 0.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.107 ± 0.005

4.011 ± 0.009

6.06 ± 0.016

3.841 ± 0.009

5.876 ± 0.011

9.134 ± 0.022

6.188 ± 0.011

6.252 ± 0.026

1.511 ± 0.005

2.763 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski