Tulasnella calospora MUT 4182

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Cantharellales; Tulasnellaceae; Tulasnella; Tulasnella calospora

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19357 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C3QP43|A0A0C3QP43_9AGAM Uncharacterized protein OS=Tulasnella calospora MUT 4182 OX=1051891 GN=M407DRAFT_21916 PE=4 SV=1
MM1 pKa = 8.34RR2 pKa = 11.84YY3 pKa = 6.27TTILVAALAIAATHH17 pKa = 6.08VAAQDD22 pKa = 3.71EE23 pKa = 4.73VDD25 pKa = 5.37DD26 pKa = 4.27PTATDD31 pKa = 3.41TADD34 pKa = 4.29GPDD37 pKa = 3.72STDD40 pKa = 4.04GPDD43 pKa = 3.5STDD46 pKa = 4.01GPDD49 pKa = 3.5STDD52 pKa = 4.01GPDD55 pKa = 3.5STDD58 pKa = 4.01GPDD61 pKa = 3.5STDD64 pKa = 3.56GPDD67 pKa = 3.06TGAIPACASTCAEE80 pKa = 4.11TSAATSGCTDD90 pKa = 3.18ATDD93 pKa = 3.89VNCLCSSTPFINAVAHH109 pKa = 7.05ADD111 pKa = 3.81DD112 pKa = 3.83QAAAAAFASEE122 pKa = 4.73FCADD126 pKa = 3.98AEE128 pKa = 4.58PTTSAPVSSTPAVTSRR144 pKa = 11.84PVTSAAVATSRR155 pKa = 11.84PAVTSAVRR163 pKa = 11.84SSASVVSRR171 pKa = 11.84ASSASGSASAAANAGTALKK190 pKa = 9.07MPSVSYY196 pKa = 10.49LALGATALGFVVGPLFLFAA215 pKa = 6.08

Molecular weight: 20.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C3QLD8|A0A0C3QLD8_9AGAM Small nuclear ribonucleoprotein E OS=Tulasnella calospora MUT 4182 OX=1051891 GN=M407DRAFT_243049 PE=3 SV=1
MM1 pKa = 7.4TPLSPMFGGPVRR13 pKa = 11.84SGGSNSNPPILLGNPRR29 pKa = 11.84VPIRR33 pKa = 11.84PRR35 pKa = 11.84GAATTPRR42 pKa = 11.84RR43 pKa = 11.84SQAGSFWGFF52 pKa = 2.99

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19349

8

19357

6612335

49

3687

341.6

37.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.615 ± 0.017

1.288 ± 0.007

5.48 ± 0.01

5.991 ± 0.019

3.613 ± 0.011

6.744 ± 0.018

2.392 ± 0.009

4.818 ± 0.013

4.583 ± 0.016

9.275 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.0 ± 0.006

3.462 ± 0.009

6.616 ± 0.025

3.844 ± 0.012

6.244 ± 0.014

8.632 ± 0.025

6.046 ± 0.017

6.131 ± 0.012

1.581 ± 0.007

2.645 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski