Amino acid dipepetide frequency for Microbacterium phage LeeroyJenkins

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.064AlaAla: 10.064 ± 1.417
0.692AlaCys: 0.692 ± 0.198
6.816AlaAsp: 6.816 ± 0.609
6.23AlaGlu: 6.23 ± 0.666
2.609AlaPhe: 2.609 ± 0.321
7.827AlaGly: 7.827 ± 1.061
1.704AlaHis: 1.704 ± 0.282
5.218AlaIle: 5.218 ± 0.516
4.1AlaLys: 4.1 ± 0.474
7.827AlaLeu: 7.827 ± 0.796
3.035AlaMet: 3.035 ± 0.413
4.26AlaAsn: 4.26 ± 0.69
3.514AlaPro: 3.514 ± 0.479
3.834AlaGln: 3.834 ± 0.547
4.952AlaArg: 4.952 ± 0.578
5.911AlaSer: 5.911 ± 0.995
6.55AlaThr: 6.55 ± 0.65
6.124AlaVal: 6.124 ± 0.688
1.97AlaTrp: 1.97 ± 0.352
2.982AlaTyr: 2.982 ± 0.383
0.0AlaXaa: 0.0 ± 0.0
Cys
0.639CysAla: 0.639 ± 0.209
0.213CysCys: 0.213 ± 0.095
0.586CysAsp: 0.586 ± 0.194
0.745CysGlu: 0.745 ± 0.291
0.266CysPhe: 0.266 ± 0.123
1.065CysGly: 1.065 ± 0.301
0.213CysHis: 0.213 ± 0.128
0.16CysIle: 0.16 ± 0.101
0.479CysLys: 0.479 ± 0.197
0.373CysLeu: 0.373 ± 0.148
0.16CysMet: 0.16 ± 0.101
0.479CysAsn: 0.479 ± 0.189
0.799CysPro: 0.799 ± 0.245
0.213CysGln: 0.213 ± 0.098
0.692CysArg: 0.692 ± 0.234
0.426CysSer: 0.426 ± 0.165
0.532CysThr: 0.532 ± 0.223
0.745CysVal: 0.745 ± 0.21
0.213CysTrp: 0.213 ± 0.108
0.319CysTyr: 0.319 ± 0.128
0.0CysXaa: 0.0 ± 0.0
Asp
6.603AspAla: 6.603 ± 0.755
0.958AspCys: 0.958 ± 0.315
4.313AspAsp: 4.313 ± 0.467
5.378AspGlu: 5.378 ± 0.611
2.503AspPhe: 2.503 ± 0.387
5.644AspGly: 5.644 ± 0.511
1.171AspHis: 1.171 ± 0.289
2.716AspIle: 2.716 ± 0.365
2.183AspLys: 2.183 ± 0.411
6.656AspLeu: 6.656 ± 0.477
2.023AspMet: 2.023 ± 0.373
3.142AspAsn: 3.142 ± 0.434
2.929AspPro: 2.929 ± 0.424
1.171AspGln: 1.171 ± 0.268
4.207AspArg: 4.207 ± 0.524
4.26AspSer: 4.26 ± 0.537
3.94AspThr: 3.94 ± 0.468
3.994AspVal: 3.994 ± 0.424
1.544AspTrp: 1.544 ± 0.271
3.088AspTyr: 3.088 ± 0.42
0.0AspXaa: 0.0 ± 0.0
Glu
7.668GluAla: 7.668 ± 0.772
0.905GluCys: 0.905 ± 0.27
4.792GluAsp: 4.792 ± 0.59
5.857GluGlu: 5.857 ± 0.735
3.301GluPhe: 3.301 ± 0.471
5.538GluGly: 5.538 ± 0.594
0.852GluHis: 0.852 ± 0.283
5.112GluIle: 5.112 ± 0.565
3.834GluLys: 3.834 ± 0.526
5.165GluLeu: 5.165 ± 0.501
2.236GluMet: 2.236 ± 0.31
2.716GluAsn: 2.716 ± 0.404
2.609GluPro: 2.609 ± 0.392
1.864GluGln: 1.864 ± 0.294
3.887GluArg: 3.887 ± 0.451
4.42GluSer: 4.42 ± 0.542
3.195GluThr: 3.195 ± 0.44
4.473GluVal: 4.473 ± 0.476
1.864GluTrp: 1.864 ± 0.371
2.503GluTyr: 2.503 ± 0.37
0.0GluXaa: 0.0 ± 0.0
Phe
3.514PheAla: 3.514 ± 0.555
0.319PheCys: 0.319 ± 0.122
2.982PheAsp: 2.982 ± 0.42
3.461PheGlu: 3.461 ± 0.451
1.278PhePhe: 1.278 ± 0.244
2.769PheGly: 2.769 ± 0.408
0.586PheHis: 0.586 ± 0.178
1.704PheIle: 1.704 ± 0.288
0.799PheLys: 0.799 ± 0.229
2.077PheLeu: 2.077 ± 0.409
0.479PheMet: 0.479 ± 0.151
1.651PheAsn: 1.651 ± 0.317
1.651PhePro: 1.651 ± 0.252
0.799PheGln: 0.799 ± 0.224
2.396PheArg: 2.396 ± 0.331
1.97PheSer: 1.97 ± 0.333
2.343PheThr: 2.343 ± 0.339
2.077PheVal: 2.077 ± 0.283
0.905PheTrp: 0.905 ± 0.226
1.331PheTyr: 1.331 ± 0.268
0.0PheXaa: 0.0 ± 0.0
Gly
6.656GlyAla: 6.656 ± 0.701
0.852GlyCys: 0.852 ± 0.229
5.751GlyAsp: 5.751 ± 0.672
5.591GlyGlu: 5.591 ± 0.554
3.94GlyPhe: 3.94 ± 0.351
6.816GlyGly: 6.816 ± 0.657
1.012GlyHis: 1.012 ± 0.244
4.526GlyIle: 4.526 ± 0.5
3.035GlyLys: 3.035 ± 0.447
6.23GlyLeu: 6.23 ± 0.581
1.651GlyMet: 1.651 ± 0.302
3.408GlyAsn: 3.408 ± 0.454
2.822GlyPro: 2.822 ± 0.68
3.142GlyGln: 3.142 ± 0.472
4.792GlyArg: 4.792 ± 0.442
4.952GlySer: 4.952 ± 0.652
6.496GlyThr: 6.496 ± 0.746
7.242GlyVal: 7.242 ± 0.579
1.491GlyTrp: 1.491 ± 0.276
3.674GlyTyr: 3.674 ± 0.432
0.0GlyXaa: 0.0 ± 0.0
His
0.905HisAla: 0.905 ± 0.196
0.479HisCys: 0.479 ± 0.183
1.438HisAsp: 1.438 ± 0.281
0.958HisGlu: 0.958 ± 0.255
0.426HisPhe: 0.426 ± 0.138
1.384HisGly: 1.384 ± 0.278
0.373HisHis: 0.373 ± 0.131
0.852HisIle: 0.852 ± 0.243
0.586HisLys: 0.586 ± 0.166
1.384HisLeu: 1.384 ± 0.288
0.266HisMet: 0.266 ± 0.12
0.319HisAsn: 0.319 ± 0.164
0.905HisPro: 0.905 ± 0.218
0.479HisGln: 0.479 ± 0.185
1.278HisArg: 1.278 ± 0.275
1.012HisSer: 1.012 ± 0.227
0.905HisThr: 0.905 ± 0.224
0.852HisVal: 0.852 ± 0.251
0.426HisTrp: 0.426 ± 0.155
0.479HisTyr: 0.479 ± 0.145
0.0HisXaa: 0.0 ± 0.0
Ile
5.485IleAla: 5.485 ± 0.73
0.373IleCys: 0.373 ± 0.163
3.674IleAsp: 3.674 ± 0.425
5.378IleGlu: 5.378 ± 0.538
1.331IlePhe: 1.331 ± 0.248
3.195IleGly: 3.195 ± 0.355
0.905IleHis: 0.905 ± 0.248
1.278IleIle: 1.278 ± 0.238
1.651IleLys: 1.651 ± 0.304
3.195IleLeu: 3.195 ± 0.382
0.958IleMet: 0.958 ± 0.235
1.864IleAsn: 1.864 ± 0.301
2.609IlePro: 2.609 ± 0.338
1.757IleGln: 1.757 ± 0.291
3.887IleArg: 3.887 ± 0.491
3.035IleSer: 3.035 ± 0.464
2.982IleThr: 2.982 ± 0.462
3.035IleVal: 3.035 ± 0.413
0.799IleTrp: 0.799 ± 0.249
1.438IleTyr: 1.438 ± 0.307
0.0IleXaa: 0.0 ± 0.0
Lys
3.887LysAla: 3.887 ± 0.454
0.373LysCys: 0.373 ± 0.153
2.556LysAsp: 2.556 ± 0.338
2.556LysGlu: 2.556 ± 0.379
1.118LysPhe: 1.118 ± 0.254
2.929LysGly: 2.929 ± 0.393
0.958LysHis: 0.958 ± 0.263
2.503LysIle: 2.503 ± 0.417
2.183LysLys: 2.183 ± 0.366
2.396LysLeu: 2.396 ± 0.314
1.278LysMet: 1.278 ± 0.285
1.171LysAsn: 1.171 ± 0.289
1.97LysPro: 1.97 ± 0.444
1.438LysGln: 1.438 ± 0.253
3.781LysArg: 3.781 ± 0.562
2.716LysSer: 2.716 ± 0.455
1.917LysThr: 1.917 ± 0.242
3.248LysVal: 3.248 ± 0.41
0.692LysTrp: 0.692 ± 0.155
1.384LysTyr: 1.384 ± 0.237
0.0LysXaa: 0.0 ± 0.0
Leu
7.721LeuAla: 7.721 ± 0.858
0.266LeuCys: 0.266 ± 0.11
5.005LeuAsp: 5.005 ± 0.422
4.899LeuGlu: 4.899 ± 0.583
1.757LeuPhe: 1.757 ± 0.271
5.857LeuGly: 5.857 ± 0.564
1.225LeuHis: 1.225 ± 0.248
3.834LeuIle: 3.834 ± 0.337
3.408LeuLys: 3.408 ± 0.384
5.165LeuLeu: 5.165 ± 0.526
1.704LeuMet: 1.704 ± 0.265
2.875LeuAsn: 2.875 ± 0.452
3.301LeuPro: 3.301 ± 0.403
2.13LeuGln: 2.13 ± 0.4
5.378LeuArg: 5.378 ± 0.602
6.23LeuSer: 6.23 ± 0.558
5.431LeuThr: 5.431 ± 0.562
4.579LeuVal: 4.579 ± 0.501
1.491LeuTrp: 1.491 ± 0.282
1.704LeuTyr: 1.704 ± 0.308
0.0LeuXaa: 0.0 ± 0.0
Met
3.248MetAla: 3.248 ± 0.395
0.106MetCys: 0.106 ± 0.082
1.065MetAsp: 1.065 ± 0.254
1.118MetGlu: 1.118 ± 0.236
0.852MetPhe: 0.852 ± 0.215
1.278MetGly: 1.278 ± 0.226
0.373MetHis: 0.373 ± 0.125
1.012MetIle: 1.012 ± 0.289
1.118MetLys: 1.118 ± 0.238
1.384MetLeu: 1.384 ± 0.272
0.586MetMet: 0.586 ± 0.197
0.852MetAsn: 0.852 ± 0.212
0.958MetPro: 0.958 ± 0.176
0.692MetGln: 0.692 ± 0.227
1.864MetArg: 1.864 ± 0.309
1.864MetSer: 1.864 ± 0.315
2.449MetThr: 2.449 ± 0.338
1.384MetVal: 1.384 ± 0.284
0.532MetTrp: 0.532 ± 0.154
0.373MetTyr: 0.373 ± 0.133
0.0MetXaa: 0.0 ± 0.0
Asn
3.727AsnAla: 3.727 ± 0.428
0.319AsnCys: 0.319 ± 0.136
2.343AsnAsp: 2.343 ± 0.325
2.023AsnGlu: 2.023 ± 0.354
1.278AsnPhe: 1.278 ± 0.203
3.621AsnGly: 3.621 ± 0.468
0.852AsnHis: 0.852 ± 0.252
1.651AsnIle: 1.651 ± 0.263
1.704AsnLys: 1.704 ± 0.338
2.982AsnLeu: 2.982 ± 0.381
1.118AsnMet: 1.118 ± 0.305
1.065AsnAsn: 1.065 ± 0.266
2.875AsnPro: 2.875 ± 0.317
1.118AsnGln: 1.118 ± 0.222
2.822AsnArg: 2.822 ± 0.449
2.769AsnSer: 2.769 ± 0.507
1.97AsnThr: 1.97 ± 0.402
2.503AsnVal: 2.503 ± 0.331
0.905AsnTrp: 0.905 ± 0.213
1.065AsnTyr: 1.065 ± 0.211
0.0AsnXaa: 0.0 ± 0.0
Pro
4.313ProAla: 4.313 ± 0.425
0.319ProCys: 0.319 ± 0.141
3.994ProAsp: 3.994 ± 0.671
3.621ProGlu: 3.621 ± 0.452
1.704ProPhe: 1.704 ± 0.309
4.26ProGly: 4.26 ± 0.56
0.639ProHis: 0.639 ± 0.182
1.97ProIle: 1.97 ± 0.337
1.917ProLys: 1.917 ± 0.283
2.183ProLeu: 2.183 ± 0.344
0.745ProMet: 0.745 ± 0.159
1.118ProAsn: 1.118 ± 0.221
1.065ProPro: 1.065 ± 0.262
1.544ProGln: 1.544 ± 0.369
1.864ProArg: 1.864 ± 0.309
2.077ProSer: 2.077 ± 0.326
3.408ProThr: 3.408 ± 0.455
3.568ProVal: 3.568 ± 0.475
0.958ProTrp: 0.958 ± 0.256
1.438ProTyr: 1.438 ± 0.216
0.0ProXaa: 0.0 ± 0.0
Gln
3.834GlnAla: 3.834 ± 0.677
0.426GlnCys: 0.426 ± 0.146
2.183GlnAsp: 2.183 ± 0.34
2.236GlnGlu: 2.236 ± 0.363
1.225GlnPhe: 1.225 ± 0.281
3.088GlnGly: 3.088 ± 0.734
0.479GlnHis: 0.479 ± 0.157
1.651GlnIle: 1.651 ± 0.312
1.118GlnLys: 1.118 ± 0.318
1.864GlnLeu: 1.864 ± 0.375
0.373GlnMet: 0.373 ± 0.17
1.012GlnAsn: 1.012 ± 0.2
1.012GlnPro: 1.012 ± 0.221
1.012GlnGln: 1.012 ± 0.437
2.023GlnArg: 2.023 ± 0.416
1.864GlnSer: 1.864 ± 0.38
1.597GlnThr: 1.597 ± 0.329
2.343GlnVal: 2.343 ± 0.37
0.745GlnTrp: 0.745 ± 0.191
1.384GlnTyr: 1.384 ± 0.337
0.0GlnXaa: 0.0 ± 0.0
Arg
5.485ArgAla: 5.485 ± 0.591
0.799ArgCys: 0.799 ± 0.251
4.153ArgAsp: 4.153 ± 0.505
5.005ArgGlu: 5.005 ± 0.71
2.183ArgPhe: 2.183 ± 0.324
5.112ArgGly: 5.112 ± 0.506
0.958ArgHis: 0.958 ± 0.257
3.568ArgIle: 3.568 ± 0.442
3.355ArgLys: 3.355 ± 0.419
4.526ArgLeu: 4.526 ± 0.395
2.449ArgMet: 2.449 ± 0.34
2.396ArgAsn: 2.396 ± 0.327
2.236ArgPro: 2.236 ± 0.326
2.023ArgGln: 2.023 ± 0.314
3.621ArgArg: 3.621 ± 0.474
2.822ArgSer: 2.822 ± 0.357
3.994ArgThr: 3.994 ± 0.446
4.686ArgVal: 4.686 ± 0.508
1.544ArgTrp: 1.544 ± 0.288
2.503ArgTyr: 2.503 ± 0.413
0.0ArgXaa: 0.0 ± 0.0
Ser
5.378SerAla: 5.378 ± 0.709
0.532SerCys: 0.532 ± 0.184
4.313SerAsp: 4.313 ± 0.367
4.1SerGlu: 4.1 ± 0.471
2.29SerPhe: 2.29 ± 0.317
6.709SerGly: 6.709 ± 0.74
0.745SerHis: 0.745 ± 0.185
2.822SerIle: 2.822 ± 0.383
2.183SerLys: 2.183 ± 0.36
5.751SerLeu: 5.751 ± 0.475
1.171SerMet: 1.171 ± 0.188
2.023SerAsn: 2.023 ± 0.317
2.556SerPro: 2.556 ± 0.378
1.704SerGln: 1.704 ± 0.375
4.153SerArg: 4.153 ± 0.462
4.1SerSer: 4.1 ± 0.754
4.047SerThr: 4.047 ± 0.515
4.42SerVal: 4.42 ± 0.518
1.171SerTrp: 1.171 ± 0.295
2.077SerTyr: 2.077 ± 0.246
0.0SerXaa: 0.0 ± 0.0
Thr
6.496ThrAla: 6.496 ± 0.691
0.426ThrCys: 0.426 ± 0.19
3.834ThrAsp: 3.834 ± 0.452
3.887ThrGlu: 3.887 ± 0.467
2.396ThrPhe: 2.396 ± 0.377
6.922ThrGly: 6.922 ± 0.601
1.118ThrHis: 1.118 ± 0.259
2.822ThrIle: 2.822 ± 0.422
2.023ThrLys: 2.023 ± 0.282
5.218ThrLeu: 5.218 ± 0.598
0.958ThrMet: 0.958 ± 0.186
3.301ThrAsn: 3.301 ± 0.363
3.514ThrPro: 3.514 ± 0.484
2.29ThrGln: 2.29 ± 0.389
3.035ThrArg: 3.035 ± 0.376
3.408ThrSer: 3.408 ± 0.449
4.686ThrThr: 4.686 ± 0.696
5.538ThrVal: 5.538 ± 0.57
1.544ThrTrp: 1.544 ± 0.342
2.662ThrTyr: 2.662 ± 0.385
0.0ThrXaa: 0.0 ± 0.0
Val
5.911ValAla: 5.911 ± 0.654
0.532ValCys: 0.532 ± 0.188
4.952ValAsp: 4.952 ± 0.718
5.751ValGlu: 5.751 ± 0.589
2.556ValPhe: 2.556 ± 0.357
5.218ValGly: 5.218 ± 0.611
0.745ValHis: 0.745 ± 0.185
2.716ValIle: 2.716 ± 0.404
2.875ValLys: 2.875 ± 0.443
5.804ValLeu: 5.804 ± 0.608
1.065ValMet: 1.065 ± 0.225
2.609ValAsn: 2.609 ± 0.491
2.929ValPro: 2.929 ± 0.44
2.236ValGln: 2.236 ± 0.33
4.473ValArg: 4.473 ± 0.48
4.633ValSer: 4.633 ± 0.444
5.378ValThr: 5.378 ± 0.581
4.899ValVal: 4.899 ± 0.538
1.491ValTrp: 1.491 ± 0.302
3.035ValTyr: 3.035 ± 0.392
0.0ValXaa: 0.0 ± 0.0
Trp
1.757TrpAla: 1.757 ± 0.273
0.106TrpCys: 0.106 ± 0.079
1.491TrpAsp: 1.491 ± 0.306
1.384TrpGlu: 1.384 ± 0.249
0.799TrpPhe: 0.799 ± 0.245
1.544TrpGly: 1.544 ± 0.29
0.319TrpHis: 0.319 ± 0.133
0.958TrpIle: 0.958 ± 0.229
0.852TrpLys: 0.852 ± 0.226
1.597TrpLeu: 1.597 ± 0.27
0.426TrpMet: 0.426 ± 0.152
1.384TrpAsn: 1.384 ± 0.278
0.799TrpPro: 0.799 ± 0.192
0.745TrpGln: 0.745 ± 0.179
1.278TrpArg: 1.278 ± 0.278
1.917TrpSer: 1.917 ± 0.315
1.331TrpThr: 1.331 ± 0.263
1.331TrpVal: 1.331 ± 0.3
0.479TrpTrp: 0.479 ± 0.216
1.118TrpTyr: 1.118 ± 0.265
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.929TyrAla: 2.929 ± 0.368
0.319TyrCys: 0.319 ± 0.144
2.183TyrAsp: 2.183 ± 0.318
2.609TyrGlu: 2.609 ± 0.371
1.278TyrPhe: 1.278 ± 0.253
3.301TyrGly: 3.301 ± 0.552
0.479TyrHis: 0.479 ± 0.165
1.757TyrIle: 1.757 ± 0.374
1.651TyrLys: 1.651 ± 0.312
2.183TyrLeu: 2.183 ± 0.341
0.373TyrMet: 0.373 ± 0.15
1.065TyrAsn: 1.065 ± 0.218
1.651TyrPro: 1.651 ± 0.317
1.278TyrGln: 1.278 ± 0.277
3.035TyrArg: 3.035 ± 0.383
1.917TyrSer: 1.917 ± 0.329
2.929TyrThr: 2.929 ± 0.434
2.769TyrVal: 2.769 ± 0.354
0.852TyrTrp: 0.852 ± 0.185
0.799TyrTyr: 0.799 ± 0.201
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 125 proteins (18781 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski