Amino acid dipepetide frequency for Streptococcus phage 7A5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.318AlaAla: 2.318 ± 0.664
0.093AlaCys: 0.093 ± 0.089
4.358AlaAsp: 4.358 ± 0.608
4.636AlaGlu: 4.636 ± 0.803
2.411AlaPhe: 2.411 ± 0.53
3.894AlaGly: 3.894 ± 0.76
0.742AlaHis: 0.742 ± 0.336
3.801AlaIle: 3.801 ± 0.716
6.675AlaLys: 6.675 ± 1.023
6.768AlaLeu: 6.768 ± 0.81
1.483AlaMet: 1.483 ± 0.36
4.821AlaAsn: 4.821 ± 0.805
1.298AlaPro: 1.298 ± 0.384
2.781AlaGln: 2.781 ± 0.45
2.874AlaArg: 2.874 ± 0.481
4.914AlaSer: 4.914 ± 0.94
3.894AlaThr: 3.894 ± 0.653
4.543AlaVal: 4.543 ± 0.678
1.02AlaTrp: 1.02 ± 0.267
2.225AlaTyr: 2.225 ± 0.432
0.0AlaXaa: 0.0 ± 0.0
Cys
0.185CysAla: 0.185 ± 0.134
0.0CysCys: 0.0 ± 0.0
0.742CysAsp: 0.742 ± 0.287
0.556CysGlu: 0.556 ± 0.287
0.371CysPhe: 0.371 ± 0.169
0.093CysGly: 0.093 ± 0.089
0.093CysHis: 0.093 ± 0.107
0.0CysIle: 0.0 ± 0.0
0.371CysLys: 0.371 ± 0.187
0.464CysLeu: 0.464 ± 0.232
0.0CysMet: 0.0 ± 0.0
0.185CysAsn: 0.185 ± 0.116
0.278CysPro: 0.278 ± 0.201
0.093CysGln: 0.093 ± 0.098
0.278CysArg: 0.278 ± 0.211
0.556CysSer: 0.556 ± 0.22
0.278CysThr: 0.278 ± 0.172
0.464CysVal: 0.464 ± 0.181
0.185CysTrp: 0.185 ± 0.121
0.278CysTyr: 0.278 ± 0.143
0.0CysXaa: 0.0 ± 0.0
Asp
4.079AspAla: 4.079 ± 0.564
0.556AspCys: 0.556 ± 0.257
3.616AspAsp: 3.616 ± 0.544
4.45AspGlu: 4.45 ± 0.8
3.894AspPhe: 3.894 ± 0.656
5.006AspGly: 5.006 ± 0.916
1.113AspHis: 1.113 ± 0.323
4.821AspIle: 4.821 ± 0.599
5.377AspLys: 5.377 ± 0.609
4.636AspLeu: 4.636 ± 0.891
2.411AspMet: 2.411 ± 0.449
4.543AspAsn: 4.543 ± 0.633
2.132AspPro: 2.132 ± 0.425
1.113AspGln: 1.113 ± 0.257
2.503AspArg: 2.503 ± 0.525
3.338AspSer: 3.338 ± 0.635
3.801AspThr: 3.801 ± 0.656
3.245AspVal: 3.245 ± 0.442
0.834AspTrp: 0.834 ± 0.276
2.781AspTyr: 2.781 ± 0.551
0.0AspXaa: 0.0 ± 0.0
Glu
4.358GluAla: 4.358 ± 0.629
0.371GluCys: 0.371 ± 0.165
3.43GluAsp: 3.43 ± 0.486
5.099GluGlu: 5.099 ± 0.909
2.225GluPhe: 2.225 ± 0.489
2.411GluGly: 2.411 ± 0.396
1.205GluHis: 1.205 ± 0.439
6.861GluIle: 6.861 ± 1.052
4.728GluLys: 4.728 ± 0.95
7.046GluLeu: 7.046 ± 0.85
1.391GluMet: 1.391 ± 0.382
3.709GluAsn: 3.709 ± 0.722
1.947GluPro: 1.947 ± 0.513
3.709GluGln: 3.709 ± 0.656
3.06GluArg: 3.06 ± 0.674
3.709GluSer: 3.709 ± 0.413
4.265GluThr: 4.265 ± 0.661
5.285GluVal: 5.285 ± 0.652
1.02GluTrp: 1.02 ± 0.265
3.523GluTyr: 3.523 ± 0.709
0.0GluXaa: 0.0 ± 0.0
Phe
3.245PheAla: 3.245 ± 0.526
0.093PheCys: 0.093 ± 0.096
4.45PheAsp: 4.45 ± 0.6
2.781PheGlu: 2.781 ± 0.462
1.947PhePhe: 1.947 ± 0.379
3.338PheGly: 3.338 ± 0.818
0.649PheHis: 0.649 ± 0.229
3.152PheIle: 3.152 ± 0.69
4.172PheLys: 4.172 ± 0.681
2.689PheLeu: 2.689 ± 0.496
0.556PheMet: 0.556 ± 0.169
3.338PheAsn: 3.338 ± 0.659
0.464PhePro: 0.464 ± 0.213
1.391PheGln: 1.391 ± 0.305
1.483PheArg: 1.483 ± 0.331
2.967PheSer: 2.967 ± 0.443
2.411PheThr: 2.411 ± 0.61
2.503PheVal: 2.503 ± 0.405
0.834PheTrp: 0.834 ± 0.279
1.762PheTyr: 1.762 ± 0.507
0.0PheXaa: 0.0 ± 0.0
Gly
3.338GlyAla: 3.338 ± 0.943
0.464GlyCys: 0.464 ± 0.211
3.801GlyAsp: 3.801 ± 0.727
3.801GlyGlu: 3.801 ± 0.618
3.43GlyPhe: 3.43 ± 0.569
4.636GlyGly: 4.636 ± 0.876
0.927GlyHis: 0.927 ± 0.295
5.006GlyIle: 5.006 ± 0.535
5.377GlyLys: 5.377 ± 0.731
5.748GlyLeu: 5.748 ± 0.757
1.576GlyMet: 1.576 ± 0.454
3.06GlyAsn: 3.06 ± 0.731
1.113GlyPro: 1.113 ± 0.265
2.596GlyGln: 2.596 ± 0.657
2.411GlyArg: 2.411 ± 0.479
4.172GlySer: 4.172 ± 0.689
4.265GlyThr: 4.265 ± 0.742
2.967GlyVal: 2.967 ± 0.595
0.927GlyTrp: 0.927 ± 0.293
3.06GlyTyr: 3.06 ± 0.514
0.0GlyXaa: 0.0 ± 0.0
His
0.464HisAla: 0.464 ± 0.21
0.0HisCys: 0.0 ± 0.0
0.742HisAsp: 0.742 ± 0.286
0.464HisGlu: 0.464 ± 0.197
0.556HisPhe: 0.556 ± 0.26
0.834HisGly: 0.834 ± 0.273
0.371HisHis: 0.371 ± 0.18
0.556HisIle: 0.556 ± 0.243
1.205HisLys: 1.205 ± 0.342
1.576HisLeu: 1.576 ± 0.365
0.464HisMet: 0.464 ± 0.266
0.927HisAsn: 0.927 ± 0.292
0.556HisPro: 0.556 ± 0.236
0.649HisGln: 0.649 ± 0.264
0.834HisArg: 0.834 ± 0.246
0.742HisSer: 0.742 ± 0.237
0.927HisThr: 0.927 ± 0.284
1.113HisVal: 1.113 ± 0.204
0.093HisTrp: 0.093 ± 0.089
0.834HisTyr: 0.834 ± 0.283
0.0HisXaa: 0.0 ± 0.0
Ile
4.358IleAla: 4.358 ± 0.752
0.556IleCys: 0.556 ± 0.196
5.192IleAsp: 5.192 ± 0.686
4.914IleGlu: 4.914 ± 1.041
2.318IlePhe: 2.318 ± 0.47
4.45IleGly: 4.45 ± 0.456
0.834IleHis: 0.834 ± 0.279
2.689IleIle: 2.689 ± 0.46
6.304IleLys: 6.304 ± 0.84
3.801IleLeu: 3.801 ± 0.639
1.762IleMet: 1.762 ± 0.468
3.245IleAsn: 3.245 ± 0.44
2.967IlePro: 2.967 ± 0.552
3.06IleGln: 3.06 ± 0.563
2.411IleArg: 2.411 ± 0.51
4.728IleSer: 4.728 ± 0.599
3.987IleThr: 3.987 ± 0.575
3.43IleVal: 3.43 ± 0.611
0.834IleTrp: 0.834 ± 0.227
2.225IleTyr: 2.225 ± 0.476
0.0IleXaa: 0.0 ± 0.0
Lys
5.563LysAla: 5.563 ± 0.848
0.464LysCys: 0.464 ± 0.256
5.563LysAsp: 5.563 ± 0.886
6.768LysGlu: 6.768 ± 1.192
3.43LysPhe: 3.43 ± 0.692
3.987LysGly: 3.987 ± 0.556
1.205LysHis: 1.205 ± 0.462
5.748LysIle: 5.748 ± 0.756
7.417LysLys: 7.417 ± 1.245
7.417LysLeu: 7.417 ± 0.896
2.411LysMet: 2.411 ± 0.608
5.377LysAsn: 5.377 ± 0.778
3.523LysPro: 3.523 ± 0.555
3.616LysGln: 3.616 ± 0.522
3.709LysArg: 3.709 ± 0.516
4.914LysSer: 4.914 ± 0.85
5.285LysThr: 5.285 ± 0.783
4.636LysVal: 4.636 ± 0.814
1.02LysTrp: 1.02 ± 0.272
3.709LysTyr: 3.709 ± 0.715
0.0LysXaa: 0.0 ± 0.0
Leu
7.046LeuAla: 7.046 ± 0.958
0.371LeuCys: 0.371 ± 0.184
5.841LeuAsp: 5.841 ± 0.635
6.397LeuGlu: 6.397 ± 0.949
2.689LeuPhe: 2.689 ± 0.363
5.192LeuGly: 5.192 ± 0.897
0.834LeuHis: 0.834 ± 0.323
5.006LeuIle: 5.006 ± 0.834
7.324LeuLys: 7.324 ± 1.03
4.821LeuLeu: 4.821 ± 0.758
2.225LeuMet: 2.225 ± 0.389
5.47LeuAsn: 5.47 ± 0.851
2.503LeuPro: 2.503 ± 0.54
2.503LeuGln: 2.503 ± 0.547
3.523LeuArg: 3.523 ± 0.816
5.006LeuSer: 5.006 ± 0.81
5.748LeuThr: 5.748 ± 1.123
3.987LeuVal: 3.987 ± 0.467
0.464LeuTrp: 0.464 ± 0.167
2.781LeuTyr: 2.781 ± 0.501
0.0LeuXaa: 0.0 ± 0.0
Met
2.132MetAla: 2.132 ± 0.386
0.0MetCys: 0.0 ± 0.0
1.02MetAsp: 1.02 ± 0.243
1.483MetGlu: 1.483 ± 0.489
1.576MetPhe: 1.576 ± 0.378
1.113MetGly: 1.113 ± 0.365
0.185MetHis: 0.185 ± 0.131
1.298MetIle: 1.298 ± 0.34
2.596MetLys: 2.596 ± 0.455
1.576MetLeu: 1.576 ± 0.267
0.742MetMet: 0.742 ± 0.259
1.205MetAsn: 1.205 ± 0.304
0.742MetPro: 0.742 ± 0.207
0.649MetGln: 0.649 ± 0.277
1.113MetArg: 1.113 ± 0.261
2.04MetSer: 2.04 ± 0.461
1.762MetThr: 1.762 ± 0.447
1.483MetVal: 1.483 ± 0.335
0.371MetTrp: 0.371 ± 0.157
0.834MetTyr: 0.834 ± 0.286
0.0MetXaa: 0.0 ± 0.0
Asn
4.543AsnAla: 4.543 ± 0.781
0.278AsnCys: 0.278 ± 0.147
3.245AsnAsp: 3.245 ± 0.425
4.265AsnGlu: 4.265 ± 0.843
3.245AsnPhe: 3.245 ± 0.592
6.861AsnGly: 6.861 ± 1.321
1.02AsnHis: 1.02 ± 0.243
3.245AsnIle: 3.245 ± 0.464
4.172AsnLys: 4.172 ± 0.616
5.099AsnLeu: 5.099 ± 0.545
1.113AsnMet: 1.113 ± 0.341
4.45AsnAsn: 4.45 ± 0.935
2.689AsnPro: 2.689 ± 0.515
2.874AsnGln: 2.874 ± 0.387
2.132AsnArg: 2.132 ± 0.496
4.265AsnSer: 4.265 ± 0.655
3.338AsnThr: 3.338 ± 0.515
3.338AsnVal: 3.338 ± 0.491
1.298AsnTrp: 1.298 ± 0.347
2.225AsnTyr: 2.225 ± 0.471
0.0AsnXaa: 0.0 ± 0.0
Pro
1.669ProAla: 1.669 ± 0.389
0.185ProCys: 0.185 ± 0.145
1.483ProAsp: 1.483 ± 0.364
2.04ProGlu: 2.04 ± 0.445
0.742ProPhe: 0.742 ± 0.294
1.113ProGly: 1.113 ± 0.38
0.464ProHis: 0.464 ± 0.19
1.298ProIle: 1.298 ± 0.283
3.245ProLys: 3.245 ± 0.577
2.503ProLeu: 2.503 ± 0.419
0.371ProMet: 0.371 ± 0.199
2.503ProAsn: 2.503 ± 0.404
0.556ProPro: 0.556 ± 0.244
1.669ProGln: 1.669 ± 0.374
1.205ProArg: 1.205 ± 0.385
2.967ProSer: 2.967 ± 0.741
2.132ProThr: 2.132 ± 0.365
1.762ProVal: 1.762 ± 0.447
0.371ProTrp: 0.371 ± 0.14
1.205ProTyr: 1.205 ± 0.428
0.0ProXaa: 0.0 ± 0.0
Gln
3.709GlnAla: 3.709 ± 0.521
0.371GlnCys: 0.371 ± 0.225
1.669GlnAsp: 1.669 ± 0.418
3.245GlnGlu: 3.245 ± 0.653
0.927GlnPhe: 0.927 ± 0.226
2.781GlnGly: 2.781 ± 0.722
0.464GlnHis: 0.464 ± 0.215
2.689GlnIle: 2.689 ± 0.523
3.152GlnLys: 3.152 ± 0.476
3.616GlnLeu: 3.616 ± 0.502
1.483GlnMet: 1.483 ± 0.355
2.781GlnAsn: 2.781 ± 0.528
0.556GlnPro: 0.556 ± 0.199
2.967GlnGln: 2.967 ± 0.502
1.391GlnArg: 1.391 ± 0.295
2.503GlnSer: 2.503 ± 0.379
2.967GlnThr: 2.967 ± 0.484
2.411GlnVal: 2.411 ± 0.647
0.834GlnTrp: 0.834 ± 0.318
1.576GlnTyr: 1.576 ± 0.371
0.0GlnXaa: 0.0 ± 0.0
Arg
2.318ArgAla: 2.318 ± 0.444
0.093ArgCys: 0.093 ± 0.098
2.596ArgAsp: 2.596 ± 0.526
2.874ArgGlu: 2.874 ± 0.584
2.596ArgPhe: 2.596 ± 0.452
2.225ArgGly: 2.225 ± 0.578
0.556ArgHis: 0.556 ± 0.234
3.152ArgIle: 3.152 ± 0.591
3.801ArgLys: 3.801 ± 0.641
3.152ArgLeu: 3.152 ± 0.501
0.834ArgMet: 0.834 ± 0.261
2.318ArgAsn: 2.318 ± 0.4
1.02ArgPro: 1.02 ± 0.246
2.225ArgGln: 2.225 ± 0.498
0.834ArgArg: 0.834 ± 0.238
2.04ArgSer: 2.04 ± 0.47
2.04ArgThr: 2.04 ± 0.631
2.503ArgVal: 2.503 ± 0.484
0.834ArgTrp: 0.834 ± 0.242
2.225ArgTyr: 2.225 ± 0.53
0.0ArgXaa: 0.0 ± 0.0
Ser
4.728SerAla: 4.728 ± 0.661
0.371SerCys: 0.371 ± 0.226
3.709SerAsp: 3.709 ± 0.504
3.338SerGlu: 3.338 ± 0.429
2.689SerPhe: 2.689 ± 0.522
4.45SerGly: 4.45 ± 0.581
0.278SerHis: 0.278 ± 0.14
4.636SerIle: 4.636 ± 0.718
6.119SerLys: 6.119 ± 1.18
5.099SerLeu: 5.099 ± 0.591
1.854SerMet: 1.854 ± 0.358
4.636SerAsn: 4.636 ± 0.695
2.04SerPro: 2.04 ± 0.346
3.245SerGln: 3.245 ± 0.61
3.245SerArg: 3.245 ± 0.623
5.006SerSer: 5.006 ± 1.144
4.358SerThr: 4.358 ± 0.896
4.45SerVal: 4.45 ± 0.718
0.834SerTrp: 0.834 ± 0.367
1.947SerTyr: 1.947 ± 0.397
0.0SerXaa: 0.0 ± 0.0
Thr
4.543ThrAla: 4.543 ± 0.759
0.278ThrCys: 0.278 ± 0.144
4.358ThrAsp: 4.358 ± 0.806
3.801ThrGlu: 3.801 ± 0.6
3.709ThrPhe: 3.709 ± 0.578
3.709ThrGly: 3.709 ± 0.53
1.113ThrHis: 1.113 ± 0.422
3.616ThrIle: 3.616 ± 0.619
6.119ThrLys: 6.119 ± 0.815
5.748ThrLeu: 5.748 ± 0.825
1.02ThrMet: 1.02 ± 0.357
3.152ThrAsn: 3.152 ± 0.544
1.391ThrPro: 1.391 ± 0.342
2.689ThrGln: 2.689 ± 0.522
2.04ThrArg: 2.04 ± 0.414
3.801ThrSer: 3.801 ± 0.741
3.616ThrThr: 3.616 ± 0.737
3.987ThrVal: 3.987 ± 0.547
1.113ThrTrp: 1.113 ± 0.308
3.152ThrTyr: 3.152 ± 0.527
0.0ThrXaa: 0.0 ± 0.0
Val
4.079ValAla: 4.079 ± 0.553
0.371ValCys: 0.371 ± 0.195
4.358ValAsp: 4.358 ± 0.613
4.543ValGlu: 4.543 ± 0.727
2.225ValPhe: 2.225 ± 0.591
3.894ValGly: 3.894 ± 0.584
0.556ValHis: 0.556 ± 0.191
3.523ValIle: 3.523 ± 0.335
4.079ValLys: 4.079 ± 0.557
3.709ValLeu: 3.709 ± 0.653
1.113ValMet: 1.113 ± 0.327
4.636ValAsn: 4.636 ± 0.77
2.132ValPro: 2.132 ± 0.403
1.762ValGln: 1.762 ± 0.375
2.503ValArg: 2.503 ± 0.671
4.265ValSer: 4.265 ± 0.632
5.006ValThr: 5.006 ± 0.912
3.801ValVal: 3.801 ± 0.533
1.02ValTrp: 1.02 ± 0.279
2.503ValTyr: 2.503 ± 0.47
0.0ValXaa: 0.0 ± 0.0
Trp
0.556TrpAla: 0.556 ± 0.224
0.0TrpCys: 0.0 ± 0.0
1.298TrpAsp: 1.298 ± 0.446
0.556TrpGlu: 0.556 ± 0.247
0.649TrpPhe: 0.649 ± 0.257
0.649TrpGly: 0.649 ± 0.26
0.556TrpHis: 0.556 ± 0.227
0.834TrpIle: 0.834 ± 0.277
0.742TrpLys: 0.742 ± 0.292
1.298TrpLeu: 1.298 ± 0.389
0.093TrpMet: 0.093 ± 0.089
0.927TrpAsn: 0.927 ± 0.369
0.093TrpPro: 0.093 ± 0.1
0.371TrpGln: 0.371 ± 0.22
0.742TrpArg: 0.742 ± 0.234
1.947TrpSer: 1.947 ± 0.677
0.742TrpThr: 0.742 ± 0.191
1.576TrpVal: 1.576 ± 0.383
0.371TrpTrp: 0.371 ± 0.218
0.371TrpTyr: 0.371 ± 0.249
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.318TyrAla: 2.318 ± 0.384
0.556TyrCys: 0.556 ± 0.313
2.781TyrAsp: 2.781 ± 0.553
3.43TyrGlu: 3.43 ± 0.579
2.596TyrPhe: 2.596 ± 0.57
2.04TyrGly: 2.04 ± 0.477
0.742TyrHis: 0.742 ± 0.254
2.132TyrIle: 2.132 ± 0.46
2.781TyrLys: 2.781 ± 0.44
2.967TyrLeu: 2.967 ± 0.523
0.927TyrMet: 0.927 ± 0.298
2.411TyrAsn: 2.411 ± 0.516
1.483TyrPro: 1.483 ± 0.423
2.132TyrGln: 2.132 ± 0.353
1.947TyrArg: 1.947 ± 0.392
3.152TyrSer: 3.152 ± 0.721
2.132TyrThr: 2.132 ± 0.484
2.596TyrVal: 2.596 ± 0.433
0.093TyrTrp: 0.093 ± 0.092
1.947TyrTyr: 1.947 ± 0.603
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 44 proteins (10787 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski